KlpnC.00101.a
Ferredoxin (flavodoxin):NADP(+) oxidoreductase
| CENTER ID: | KlpnC.00101.a |
| ORGANISM: | Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.00101.a.B1.GE44209 | Full length(KlpnC.00101.a) | 1 | 248 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlpnC.00101.a.B1.PS38693 | Full length(KlpnC.00101.a) | 1 | 248 | ||
| KlpnC.00101.a.B1.PS38694 | Full length(KlpnC.00101.a) | 1 | 248 |
Structures
8DOS
DEPOSITED: 7/14/2022
DETERMINATION: XRay
CLONE: KlpnC.00101.a.B1.GE44209
PROTEIN: KlpnC.00101.a.B1.PS38694
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1125630.4.peg.5053 |
| UniProt: | A0A0H3H078 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADWVSGKVT KVEYWTDALF SLYVRAPVHP FTAGQFTKLG LEIDGERVQR AYSYVNAPGN PDLEFYLVTV PEGKLSPRLA SLKPGDEVLV VSEAAGFFVL EEVPDCDTLW MLATGTALGP YLSILQEGKD LERFNNLVLV HAVRYAADLS YLPLMRELEQ RYAGKLRIQT VVSRETVEGS LTGRVPFLIE TGALEEAVGL PMTTDTSHVM LCGNPQMVRD TQQLLKETRQ MTKHLRRRPG HMTAEHYW
NT Sequence
ATGGCGGACT GGGTTAGCGG CAAAGTGACA AAAGTGGAGT ACTGGACGGA CGCCTTGTTC AGTTTATACG TGCGGGCGCC TGTTCATCCG TTTACCGCCG GGCAATTTAC CAAGCTCGGG CTGGAAATCG ACGGCGAACG CGTGCAGCGC GCCTACTCCT ACGTCAACGC CCCCGGCAAT CCCGACCTCG AGTTCTATTT GGTCACCGTC CCGGAAGGCA AGCTGAGCCC GCGTCTGGCG TCGCTGAAGC CGGGCGATGA AGTCCTGGTG GTGAGTGAAG CGGCAGGTTT CTTCGTCCTC GAGGAGGTAC CGGATTGCGA TACCCTGTGG ATGCTGGCGA CCGGCACTGC GCTGGGGCCG TACCTGTCAA TTTTGCAGGA AGGCAAGGAC CTTGAGCGCT TTAATAACCT GGTGCTGGTC CACGCCGTCC GCTACGCCGC GGACCTGAGC TATCTGCCGC TAATGCGCGA ACTGGAGCAG CGCTATGCCG GCAAACTCCG TATTCAGACG GTGGTCAGCC GCGAAACCGT GGAAGGCTCG CTGACCGGCC GGGTGCCGTT TCTGATTGAA ACCGGCGCGC TGGAAGAGGC CGTCGGCCTG CCGATGACCA CCGACACCAG CCATGTGATG CTGTGCGGCA ACCCGCAAAT GGTGCGCGAT ACCCAACAGC TGTTAAAAGA GACCCGGCAG ATGACCAAAC ATCTGCGTCG CCGGCCGGGC CATATGACGG CGGAGCACTA CTGG
Details for KlpnC.00101.a.B1.GE44209
| HARVESTED ON: | 4/1/2021 |
| SEQUENCED ON: | 4/13/2021 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMA DWVSGKVTKV EYWTDALFSL YVRAPVHPFT AGQFTKLGLE IDGERVQRAY SYVNAPGNPD LEFYLVTVPE GKLSPRLAAL KPGDEVLVVS EAAGFFVLEE VPDCDTLWML ATGTALGPYL SILQEGKDLE RFNNLVLVHA VRYAADLSYL PLMRELEQRY AGKLRIQTVV SRETVEGSLT GRVPFLIETG ALEEAVGLPM TTDTSHVMLC GNPQMVRDTQ QLLKETRQMT KHLRRRPX
Validated NT Sequence
ggnctggccg gcgacgcaga tgtttggtca tctgccgggt ctcttttaac agctgttggg tatcgcgcac catttgcggg ttgccgcaca gcatcacatg gctggtgtcg gtggtcatcg gcaggccgac ggcctcttcc agcgcgccgg tttcaatcag aaacggcacc cggccggtca gcgagccttc cacggtttcg cggctgacca ccgtctggat gcgtaattta ccggcgtagc gctgctccag ttcgcgcatt agcggcagat agctcaggtc cgcggcgtag cggacggcgt ggaccagcac caggttatta aagcgctcaa ggtccttgcc ttcctgcaaa attgacaggt acggccccag cgcagtgccg gtcgccagca tccacagggt atcgcaatcc ggtacttcct cgaggacgaa gaaacctgcc gcttcactca ccaccaggac ttcatcgccc ggcttcagcg ccgccagacg cgggctcagc ttgccttccg ggacggtgac caaatagaac tcgaggtcgg gattgccggg ggcgttgacg taggagtagg cgcgctgcac gcgttcgccg tcgatttcca gcccgagctt ggtaaattgc ccggcggtaa acggatgaac aggcgcccgc acgtataaac tgaacaaggc gtccgtccag tactccactt ttgtcacttt gccgctaacc cagtccgcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMA DWVSGKVTKV EYWTDALFSL YVRAPVHPFT AGQFTKLGLE IDGERVQRAY SYVNAPGNPD LEFYLVTVPE GKLSPRLASL KPGDEVLVVS EAAGFFVLEE VPDCDTLWML ATGTALGPYL SILQEGKDLE RFNNLVLVHA VRYAADLSYL PLMRELEQRY AGKLRIQTVV SRETVEGSLT GRVPFLIETG ALEEAVGLPM TTDTSHVMLC GNPQMVRDTQ QLLKETRQMT KHLRRRPGHM TAEHYW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg actgggttag cggcaaagtg acaaaagtgg agtactggac ggacgccttg ttcagtttat acgtgcgggc gcctgttcat ccgtttaccg ccgggcaatt taccaagctc gggctggaaa tcgacggcga acgcgtgcag cgcgcctact cctacgtcaa cgcccccggc aatcccgacc tcgagttcta tttggtcacc gtcccggaag gcaagctgag cccgcgtctg gcgtcgctga agccgggcga tgaagtcctg gtggtgagtg aagcggcagg tttcttcgtc ctcgaggagg taccggattg cgataccctg tggatgctgg cgaccggcac tgcgctgggg ccgtacctgt caattttgca ggaaggcaag gaccttgagc gctttaataa cctggtgctg gtccacgccg tccgctacgc cgcggacctg agctatctgc cgctaatgcg cgaactggag cagcgctatg ccggcaaact ccgtattcag acggtggtca gccgcgaaac cgtggaaggc tcgctgaccg gccgggtgcc gtttctgatt gaaaccggcg cgctggaaga ggccgtcggc ctgccgatga ccaccgacac cagccatgtg atgctgtgcg gcaacccgca aatggtgcgc gatacccaac agctgttaaa agagacccgg cagatgacca aacatctgcg tcgccggccg ggccatatga cggcggagca ctactggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for KlpnC.00101.a.B1.PS38693
| PURIFICATION DATe: | 9/15/2021 |
| CONCENTRATION: | 22.86mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DWVSGKVTKV EYWTDALFSL YVRAPVHPFT AGQFTKLGLE IDGERVQRAY SYVNAPGNPD LEFYLVTVPE GKLSPRLAAL KPGDEVLVVS EAAGFFVLEE VPDCDTLWML ATGTALGPYL SILQEGKDLE RFNNLVLVHA VRYAADLSYL PLMRELEQRY AGKLRIQTVV SRETVEGSLT GRVPFLIETG ALEEAVGLPM TTDTSHVMLC GNPQMVRDTQ QLLKETRQMT KHLRRRPX
Validated NT Sequence
ggnctggccg gcgacgcaga tgtttggtca tctgccgggt ctcttttaac agctgttggg tatcgcgcac catttgcggg ttgccgcaca gcatcacatg gctggtgtcg gtggtcatcg gcaggccgac ggcctcttcc agcgcgccgg tttcaatcag aaacggcacc cggccggtca gcgagccttc cacggtttcg cggctgacca ccgtctggat gcgtaattta ccggcgtagc gctgctccag ttcgcgcatt agcggcagat agctcaggtc cgcggcgtag cggacggcgt ggaccagcac caggttatta aagcgctcaa ggtccttgcc ttcctgcaaa attgacaggt acggccccag cgcagtgccg gtcgccagca tccacagggt atcgcaatcc ggtacttcct cgaggacgaa gaaacctgcc gcttcactca ccaccaggac ttcatcgccc ggcttcagcg ccgccagacg cgggctcagc ttgccttccg ggacggtgac caaatagaac tcgaggtcgg gattgccggg ggcgttgacg taggagtagg cgcgctgcac gcgttcgccg tcgatttcca gcccgagctt ggtaaattgc ccggcggtaa acggatgaac aggcgcccgc acgtataaac tgaacaaggc gtccgtccag tactccactt ttgtcacttt gccgctaacc cagtccgcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMA DWVSGKVTKV EYWTDALFSL YVRAPVHPFT AGQFTKLGLE IDGERVQRAY SYVNAPGNPD LEFYLVTVPE GKLSPRLASL KPGDEVLVVS EAAGFFVLEE VPDCDTLWML ATGTALGPYL SILQEGKDLE RFNNLVLVHA VRYAADLSYL PLMRELEQRY AGKLRIQTVV SRETVEGSLT GRVPFLIETG ALEEAVGLPM TTDTSHVMLC GNPQMVRDTQ QLLKETRQMT KHLRRRPGHM TAEHYW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG ACTGGGTTAG CGGCAAAGTG ACAAAAGTGG AGTACTGGAC GGACGCCTTG TTCAGTTTAT ACGTGCGGGC GCCTGTTCAT CCGTTTACCG CCGGGCAATT TACCAAGCTC GGGCTGGAAA TCGACGGCGA ACGCGTGCAG CGCGCCTACT CCTACGTCAA CGCCCCCGGC AATCCCGACC TCGAGTTCTA TTTGGTCACC GTCCCGGAAG GCAAGCTGAG CCCGCGTCTG GCGTCGCTGA AGCCGGGCGA TGAAGTCCTG GTGGTGAGTG AAGCGGCAGG TTTCTTCGTC CTCGAGGAGG TACCGGATTG CGATACCCTG TGGATGCTGG CGACCGGCAC TGCGCTGGGG CCGTACCTGT CAATTTTGCA GGAAGGCAAG GACCTTGAGC GCTTTAATAA CCTGGTGCTG GTCCACGCCG TCCGCTACGC CGCGGACCTG AGCTATCTGC CGCTAATGCG CGAACTGGAG CAGCGCTATG CCGGCAAACT CCGTATTCAG ACGGTGGTCA GCCGCGAAAC CGTGGAAGGC TCGCTGACCG GCCGGGTGCC GTTTCTGATT GAAACCGGCG CGCTGGAAGA GGCCGTCGGC CTGCCGATGA CCACCGACAC CAGCCATGTG ATGCTGTGCG GCAACCCGCA AATGGTGCGC GATACCCAAC AGCTGTTAAA AGAGACCCGG CAGATGACCA AACATCTGCG TCGCCGGCCG GGCCATATGA CGGCGGAGCA CTACTGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for KlpnC.00101.a.B1.PS38694
| PURIFICATION DATe: | 9/15/2021 |
| CONCENTRATION: | 21.66mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DWVSGKVTKV EYWTDALFSL YVRAPVHPFT AGQFTKLGLE IDGERVQRAY SYVNAPGNPD LEFYLVTVPE GKLSPRLAAL KPGDEVLVVS EAAGFFVLEE VPDCDTLWML ATGTALGPYL SILQEGKDLE RFNNLVLVHA VRYAADLSYL PLMRELEQRY AGKLRIQTVV SRETVEGSLT GRVPFLIETG ALEEAVGLPM TTDTSHVMLC GNPQMVRDTQ QLLKETRQMT KHLRRRPX
Validated NT Sequence
ggnctggccg gcgacgcaga tgtttggtca tctgccgggt ctcttttaac agctgttggg tatcgcgcac catttgcggg ttgccgcaca gcatcacatg gctggtgtcg gtggtcatcg gcaggccgac ggcctcttcc agcgcgccgg tttcaatcag aaacggcacc cggccggtca gcgagccttc cacggtttcg cggctgacca ccgtctggat gcgtaattta ccggcgtagc gctgctccag ttcgcgcatt agcggcagat agctcaggtc cgcggcgtag cggacggcgt ggaccagcac caggttatta aagcgctcaa ggtccttgcc ttcctgcaaa attgacaggt acggccccag cgcagtgccg gtcgccagca tccacagggt atcgcaatcc ggtacttcct cgaggacgaa gaaacctgcc gcttcactca ccaccaggac ttcatcgccc ggcttcagcg ccgccagacg cgggctcagc ttgccttccg ggacggtgac caaatagaac tcgaggtcgg gattgccggg ggcgttgacg taggagtagg cgcgctgcac gcgttcgccg tcgatttcca gcccgagctt ggtaaattgc ccggcggtaa acggatgaac aggcgcccgc acgtataaac tgaacaaggc gtccgtccag tactccactt ttgtcacttt gccgctaacc cagtccgcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMA DWVSGKVTKV EYWTDALFSL YVRAPVHPFT AGQFTKLGLE IDGERVQRAY SYVNAPGNPD LEFYLVTVPE GKLSPRLASL KPGDEVLVVS EAAGFFVLEE VPDCDTLWML ATGTALGPYL SILQEGKDLE RFNNLVLVHA VRYAADLSYL PLMRELEQRY AGKLRIQTVV SRETVEGSLT GRVPFLIETG ALEEAVGLPM TTDTSHVMLC GNPQMVRDTQ QLLKETRQMT KHLRRRPGHM TAEHYW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG ACTGGGTTAG CGGCAAAGTG ACAAAAGTGG AGTACTGGAC GGACGCCTTG TTCAGTTTAT ACGTGCGGGC GCCTGTTCAT CCGTTTACCG CCGGGCAATT TACCAAGCTC GGGCTGGAAA TCGACGGCGA ACGCGTGCAG CGCGCCTACT CCTACGTCAA CGCCCCCGGC AATCCCGACC TCGAGTTCTA TTTGGTCACC GTCCCGGAAG GCAAGCTGAG CCCGCGTCTG GCGTCGCTGA AGCCGGGCGA TGAAGTCCTG GTGGTGAGTG AAGCGGCAGG TTTCTTCGTC CTCGAGGAGG TACCGGATTG CGATACCCTG TGGATGCTGG CGACCGGCAC TGCGCTGGGG CCGTACCTGT CAATTTTGCA GGAAGGCAAG GACCTTGAGC GCTTTAATAA CCTGGTGCTG GTCCACGCCG TCCGCTACGC CGCGGACCTG AGCTATCTGC CGCTAATGCG CGAACTGGAG CAGCGCTATG CCGGCAAACT CCGTATTCAG ACGGTGGTCA GCCGCGAAAC CGTGGAAGGC TCGCTGACCG GCCGGGTGCC GTTTCTGATT GAAACCGGCG CGCTGGAAGA GGCCGTCGGC CTGCCGATGA CCACCGACAC CAGCCATGTG ATGCTGTGCG GCAACCCGCA AATGGTGCGC GATACCCAAC AGCTGTTAAA AGAGACCCGG CAGATGACCA AACATCTGCG TCGCCGGCCG GGCCATATGA CGGCGGAGCA CTACTGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT