KlpnC.00100.a

Autoinducer 2-binding protein LsrB

CENTER ID: KlpnC.00100.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00100.a.B2.GE44208 Mature protein(KlpnC.00100.a) 19 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4528
UniProt: A0A0H3GTJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLKLIVLAL AMSVVTAQAA DRIAFIPKLV GVGFFTSGGN GAKEAGKALG VDVTYDGPTE PSVSGQVQLI NNFVNQGYNA IIVSAVSPDG LCPALKRAMQ RGVKVLTWDS DTRPECRSIY INQGTPQQLG GLLVEMAEKQ VTKPTAKVAF FYSSPTVTDQ NQWVKEAKAK IEKEHPQWQI VTTQFGYNDA TKSLQTAEGI LKAYPDLDAI IAPDANALPA AAQAAENLKR QGVAIVGFST PNVMRPYVER GTVKAFGLWD VVQQGKIAVN VADRLLKKGD LNVGDSVEVK DIGSLKVEPN SVQGYQYEAK GNGIVLLPER VVFSKENINN YDF
NT Sequence
ATGAAATTAA AACTGATCGT GCTGGCGCTG GCGATGAGCG TGGTTACCGC GCAGGCGGCG GACCGCATCG CCTTTATTCC TAAGCTGGTC GGCGTCGGCT TCTTTACCAG CGGCGGCAAC GGCGCGAAAG AGGCGGGGAA AGCGCTGGGC GTGGATGTCA CCTATGACGG CCCCACCGAG CCCAGCGTCT CCGGCCAGGT GCAGCTTATC AACAACTTCG TCAACCAGGG CTACAACGCG ATTATCGTTT CCGCCGTCTC GCCGGACGGC CTGTGTCCGG CGCTGAAGCG GGCGATGCAG CGCGGGGTGA AAGTCCTGAC CTGGGATTCC GATACCAGGC CGGAGTGCCG CAGCATCTAT ATCAATCAGG GCACGCCGCA GCAGCTTGGC GGCCTGCTGG TCGAGATGGC GGAAAAGCAG GTGACGAAGC CTACTGCCAA GGTAGCGTTT TTCTACTCCA GCCCGACGGT GACCGACCAG AACCAGTGGG TGAAGGAAGC CAAAGCCAAA ATCGAAAAAG AGCATCCGCA GTGGCAAATC GTCACCACTC AGTTCGGCTA TAACGATGCC ACCAAATCGC TGCAAACCGC CGAGGGGATC TTAAAAGCCT ATCCGGATCT CGACGCCATT ATCGCGCCGG ATGCCAATGC GCTGCCGGCG GCGGCGCAGG CGGCGGAGAA CCTCAAGCGT CAGGGCGTGG CGATCGTCGG CTTTAGTACG CCGAACGTGA TGCGCCCGTA CGTTGAGCGC GGCACGGTGA AAGCGTTCGG CCTGTGGGAT GTGGTTCAGC AGGGCAAAAT TGCGGTTAAC GTCGCCGATC GATTGCTGAA AAAGGGCGAT CTTAACGTAG GCGACAGCGT TGAGGTGAAA GATATCGGCT CGCTGAAGGT CGAACCGAAT AGCGTGCAGG GCTACCAGTA TGAGGCGAAG GGCAACGGTA TCGTGCTGCT GCCGGAGCGC GTGGTGTTCA GCAAAGAGAA CATCAACAAC TACGACTTC
Details for KlpnC.00100.a.B2.GE44208
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ADRIAFIPKL VGVGFFTSGG NGAKEAGKAL GVDVTYDGPT EPSVSGQVQL INNFVNQGYN AIIVSAVSPD GLCPALKRAM QRGVKVLTWD SDTKPECRSI YINQGTPQQL GGLLVEMAEK QVSKPAAKVA FFYSSPTVTD QNQWVKEAKA KIEKEHPQWQ IVTTQFGYND ATKSLQTAEG ILKAYPDLDA IIAPDANALP AAAQAAENLK RQGVAIVGFS TPNVMRPYVE RGTVKAFGLW DVVKQGKIAV NVADRLLKKG DLNVGDSVEV KDIGSLKVEP NSVQGYQYEA KGNGIVLLPE RVVFSKXXXN NYDF
Validated NT Sequence
cacttagaag tcgtagttgt tgannntnnn tttgctgaac accacgcgct ccggcagcag cacgataccg ttgcccttcg cttcatactg gtagccctgc acgctattcg gttcaacctt cagcgagccg atatctttca cctcaacgct atcgcctacg ttaagatcgc cctttttcag caatcgatcg gcgacgttaa ccgcaatttt gccctgctta accacatccc acaggccgaa cgctttcacc gtgccgcgct caacgtacgg gcgcatcacg ttcggagtac taaagccgac gatcgccacg ccctgacgct tgaggttctc cgccgcctgc gccgccgccg gcagcgcatt ggcatccggc gcgataatgg cgtcgagatc cggatacgct tttaagatcc cctcggcggt ttgcagcgat ttggtggcat cgttatagcc aaactgggtg gtgacgattt gccactgcgg atgctctttt tcgattttgg ctttggcttc cttcacccac tggttctggt cggtcaccgt cgggctggag taaaaaaacg ccactttggc ggcaggtttg ctgacctgct tttccgccat ctccaccagc aggccaccga gctgctgcgg cgtgccctgg ttgatataga tgctgcggca ctccggtttg gtatcggaat cccaggtcag gactttcacc ccgcgctgca tcgcccgctt cagcgccggg cacaggccgt ccggcgagac ggcggagacg ataatcgcgt tgtagccctg gttgacgaag ttgttgataa gctgcacctg cccggagacg ctgggctcgg tcgggccgtc ataggtgaca tcaacgccca gcgctttccc cgcctctttc gcgccgttgc cgccgctggt aaagaagccg acgccaacca gcttcggaat aaaggcgatg cggtccgccg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHAA DRIAFIPKLV GVGFFTSGGN GAKEAGKALG VDVTYDGPTE PSVSGQVQLI NNFVNQGYNA IIVSAVSPDG LCPALKRAMQ RGVKVLTWDS DTRPECRSIY INQGTPQQLG GLLVEMAEKQ VTKPTAKVAF FYSSPTVTDQ NQWVKEAKAK IEKEHPQWQI VTTQFGYNDA TKSLQTAEGI LKAYPDLDAI IAPDANALPA AAQAAENLKR QGVAIVGFST PNVMRPYVER GTVKAFGLWD VVQQGKIAVN VADRLLKKGD LNVGDSVEVK DIGSLKVEPN SVQGYQYEAK GNGIVLLPER VVFSKENINN YDF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcggcgg accgcatcgc ctttattcct aagctggtcg gcgtcggctt ctttaccagc ggcggcaacg gcgcgaaaga ggcggggaaa gcgctgggcg tggatgtcac ctatgacggc cccaccgagc ccagcgtctc cggccaggtg cagcttatca acaacttcgt caaccagggc tacaacgcga ttatcgtttc cgccgtctcg ccggacggcc tgtgtccggc gctgaagcgg gcgatgcagc gcggggtgaa agtcctgacc tgggattccg ataccaggcc ggagtgccgc agcatctata tcaatcaggg cacgccgcag cagcttggcg gcctgctggt cgagatggcg gaaaagcagg tgacgaagcc tactgccaag gtagcgtttt tctactccag cccgacggtg accgaccaga accagtgggt gaaggaagcc aaagccaaaa tcgaaaaaga gcatccgcag tggcaaatcg tcaccactca gttcggctat aacgatgcca ccaaatcgct gcaaaccgcc gaggggatct taaaagccta tccggatctc gacgccatta tcgcgccgga tgccaatgcg ctgccggcgg cggcgcaggc ggcggagaac ctcaagcgtc agggcgtggc gatcgtcggc tttagtacgc cgaacgtgat gcgcccgtac gttgagcgcg gcacggtgaa agcgttcggc ctgtgggatg tggttcagca gggcaaaatt gcggttaacg tcgccgatcg attgctgaaa aagggcgatc ttaacgtagg cgacagcgtt gaggtgaaag atatcggctc gctgaaggtc gaaccgaata gcgtgcaggg ctaccagtat gaggcgaagg gcaacggtat cgtgctgctg ccggagcgcg tggtgttcag caaagagaac atcaacaact acgacttctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat