SSGCID: Target Page

KlpnC.00082.a

Porphobilinogen deaminase

CENTER ID:	KlpnC.00082.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00082.a.B1.GE44203	Full length(KlpnC.00082.a)		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00082.a.B1.PS38684	Full length(KlpnC.00082.a)		1	313

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.147
UniProt:	A0A0H3GKD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDKVLKIAT RQSPLALWQA QYVKARLEQA HPGLKVELVP MVTRGDVILD TPLAKVGGKG LFVKELELAM LEGRADIAVH SMKDVPVEFP EGLGLVTICE RDDPRDAFVS NRYASIDELP AGSVVGTSSL RRQCQLAATR PDLAIRSLRG NVGTRLSKLD NGEYDAIILA AAGLKRLQLE ARIRQPLSPE QSLPAVGQGA VGIECRLDDA WTRGLLAPLN HTETAVRVRA ERAMNTRLEG GCQVPIGSYA ELKDGELWLR ALVGAPDGSQ LVRGERRGPA EQAEALGISL AEELLDNGAR EILAAVYDGE APR

NT Sequence

ATGTTAGACA AAGTTTTGAA AATTGCCACA CGGCAAAGCC CGCTTGCGCT ATGGCAGGCG CAGTATGTTA AAGCGCGCCT GGAACAAGCG CATCCCGGAC TGAAGGTCGA GCTGGTGCCG ATGGTCACGC GCGGGGATGT CATCCTTGAT ACTCCGCTGG CAAAGGTGGG GGGCAAAGGC TTATTTGTGA AAGAGTTAGA GCTCGCCATG CTCGAAGGGC GCGCCGATAT CGCCGTCCAT TCGATGAAAG ATGTGCCGGT GGAATTCCCG GAGGGCCTGG GGCTGGTGAC TATCTGCGAG CGCGACGATC CGCGCGACGC GTTTGTCTCC AATCGCTATG CCTCGATTGA CGAACTGCCG GCCGGCAGCG TCGTTGGCAC GTCAAGTTTA CGCCGTCAGT GCCAGCTGGC CGCCACCCGT CCGGACCTGG CGATCCGCTC TCTGCGCGGT AACGTCGGTA CCCGGCTGAG TAAGCTGGAT AACGGCGAGT ACGACGCCAT CATCCTCGCC GCCGCCGGCC TGAAGCGCCT GCAGCTGGAA GCGCGTATTC GCCAGCCGCT GTCGCCTGAG CAATCTCTGC CGGCGGTCGG CCAGGGCGCG GTCGGCATCG AATGCCGTCT TGATGATGCA TGGACCCGGG GGCTGTTAGC GCCTCTCAAC CATACGGAAA CGGCGGTGCG CGTGCGCGCC GAGCGGGCGA TGAACACCCG CCTCGAAGGC GGCTGCCAGG TGCCGATCGG CAGCTATGCG GAACTGAAGG ACGGGGAACT GTGGCTGCGG GCGCTGGTTG GCGCTCCGGA TGGTTCACAG CTGGTGCGCG GCGAACGACG CGGGCCAGCG GAACAGGCGG AAGCCCTCGG CATCTCGCTG GCGGAAGAGC TTCTGGATAA TGGCGCCCGC GAGATTCTCG CGGCGGTTTA TGATGGAGAA GCCCCGCGA

Details for KlpnC.00082.a.B1.GE44203

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML DKVLKIATRQ SPLALWQAQY VKARLEQAHP GLKVELVPMV TRGDVILDTP LAKVGGKGLF VKELELAMLE GRADIAVHSM KDVPVEFPEG LGLVTICERD DPRDAFVSNR YASIDELPAG SVVGTSSLRR QCQLAATRPD LAIRSLRGNV GTRLSKLDNG EYDAIILAAA GLKRLQLEAR IRQPLSPEQS LPAVGQGAVG IECRLDDAWT RGLLAPLNHT ETAVRVRAER AMNTRLEGGC QVPIGSYAEL KDGELWLRAL VGAPDGSQLV RGERRGPAEQ AEALGISLAE ELLDNGAREI LAAVYDGEAP R

Validated NT Sequence

tactcactta tcgcggggct tctccatcat aaaccgccgc gagaatctcg cgggcgccat tatccagaag ctcttccgcc agcgagatgc cgagggcttc cgcttgttcc gctggcccgc gtcgttcgcc gcgcaccagc tgtgaaccat ccggagcgcc aaccagcgcc cgcagccaca gttccccgtc cttcagttcc gcatagctgc cgatcggcac ctggcagccg ccttcgaggc gggtgttcat cgcccgctcg gcgcgcacgc gcaccgccgt ttccgtatgg ttgagaggcg ctaacagccc ccgggtccat gcatcatcaa gacggcattc gatgccgacc gcgccctggc cgaccgccgg cagagattgc tcaggcgaca gcggctggcg aatacgcgct tccagctgca ggcgcttcag gccggcggcg gcgaggatga tggcgtcgta ctcgccgtta tccagcttac tcagccgggt accgacgtta ccgcgcagag agcggatcgc caggtccgga cgggtggcgg ccagctggca ctgacggcgt aaacttgacg tgccaacgac gctgccggcc ggcagttcgt caatcgaggc atagcgattg gagacaaacg cgtcgcgcgg atcgtcgcgc tcgcagatag tcaccagccc caggccctcc gggaattcca ccggcacatc tttcatcgaa tggacggcga tatcggcgcg cccttcgagc atggcgagct ctaactcttt cacaaataag ccttttcccc ccacctttgc cagcggagta tcaaggatga catccccgcg cgtgaccatc ggcaccagct cgaccttcag tccgggatgc gcttgttcca ggcgcgcttt aacatactgc gcctgccata gcgcaagcgg gctttgccgt gtggcaattt tcaaaacttt gtctaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag acaaagtttt gaaaattgcc acacggcaaa gcccgcttgc gctatggcag gcgcagtatg ttaaagcgcg cctggaacaa gcgcatcccg gactgaaggt cgagctggtg ccgatggtca cgcgcgggga tgtcatcctt gatactccgc tggcaaaggt ggggggcaaa ggcttatttg tgaaagagtt agagctcgcc atgctcgaag ggcgcgccga tatcgccgtc cattcgatga aagatgtgcc ggtggaattc ccggagggcc tggggctggt gactatctgc gagcgcgacg atccgcgcga cgcgtttgtc tccaatcgct atgcctcgat tgacgaactg ccggccggca gcgtcgttgg cacgtcaagt ttacgccgtc agtgccagct ggccgccacc cgtccggacc tggcgatccg ctctctgcgc ggtaacgtcg gtacccggct gagtaagctg gataacggcg agtacgacgc catcatcctc gccgccgccg gcctgaagcg cctgcagctg gaagcgcgta ttcgccagcc gctgtcgcct gagcaatctc tgccggcggt cggccagggc gcggtcggca tcgaatgccg tcttgatgat gcatggaccc gggggctgtt agcgcctctc aaccatacgg aaacggcggt gcgcgtgcgc gccgagcggg cgatgaacac ccgcctcgaa ggcggctgcc aggtgccgat cggcagctat gcggaactga aggacgggga actgtggctg cgggcgctgg ttggcgctcc ggatggttca cagctggtgc gcggcgaacg acgcgggcca gcggaacagg cggaagccct cggcatctcg ctggcggaag agcttctgga taatggcgcc cgcgagattc tcgcggcggt ttatgatgga gaagccccgc gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for KlpnC.00082.a.B1.PS38684

PURIFICATION DATe:	8/11/2021
CONCENTRATION:	12.614mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAG ACAAAGTTTT GAAAATTGCC ACACGGCAAA GCCCGCTTGC GCTATGGCAG GCGCAGTATG TTAAAGCGCG CCTGGAACAA GCGCATCCCG GACTGAAGGT CGAGCTGGTG CCGATGGTCA CGCGCGGGGA TGTCATCCTT GATACTCCGC TGGCAAAGGT GGGGGGCAAA GGCTTATTTG TGAAAGAGTT AGAGCTCGCC ATGCTCGAAG GGCGCGCCGA TATCGCCGTC CATTCGATGA AAGATGTGCC GGTGGAATTC CCGGAGGGCC TGGGGCTGGT GACTATCTGC GAGCGCGACG ATCCGCGCGA CGCGTTTGTC TCCAATCGCT ATGCCTCGAT TGACGAACTG CCGGCCGGCA GCGTCGTTGG CACGTCAAGT TTACGCCGTC AGTGCCAGCT GGCCGCCACC CGTCCGGACC TGGCGATCCG CTCTCTGCGC GGTAACGTCG GTACCCGGCT GAGTAAGCTG GATAACGGCG AGTACGACGC CATCATCCTC GCCGCCGCCG GCCTGAAGCG CCTGCAGCTG GAAGCGCGTA TTCGCCAGCC GCTGTCGCCT GAGCAATCTC TGCCGGCGGT CGGCCAGGGC GCGGTCGGCA TCGAATGCCG TCTTGATGAT GCATGGACCC GGGGGCTGTT AGCGCCTCTC AACCATACGG AAACGGCGGT GCGCGTGCGC GCCGAGCGGG CGATGAACAC CCGCCTCGAA GGCGGCTGCC AGGTGCCGAT CGGCAGCTAT GCGGAACTGA AGGACGGGGA ACTGTGGCTG CGGGCGCTGG TTGGCGCTCC GGATGGTTCA CAGCTGGTGC GCGGCGAACG ACGCGGGCCA GCGGAACAGG CGGAAGCCCT CGGCATCTCG CTGGCGGAAG AGCTTCTGGA TAATGGCGCC CGCGAGATTC TCGCGGCGGT TTATGATGGA GAAGCCCCGC GATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT