KlpnC.00062.b

Inosine-5'-monophosphate dehydrogenase

CENTER ID: KlpnC.00062.b
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00062.b.B1.GE44202 Full length(KlpnC.00062.b) 1 358
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3813
UniProt: A0A0H3GW25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRIAKEALT FDDVLLVPAH STVLPNTADL STQLTKTIRL NIPMLSAAMD TVTEARLAIA LAQEGGIGFI HKNMSIERQA EEVRRVKKHE SGVVTDPQTV LPTTTLREVK ELTERNGFAG YPVVTEENEL VGIITGRDVR FVTDLNQPVS VYMTPKERLV TVREGESREV VFAKMHEKRV EKALVVDESF HLRGMITVKD FQKAERKPNA CKDEQGRLRV GAAVGAGAGN EERVDALVAA GVDVLLIDSS HGHSEGVLQR IRETRAKYPD LQIIGGNVAT GAGARALAEA GCSAVKVGIG PGLHLYYPVS SPAWVCRRSL LFPTRWKRWK GPVFRLSLTA VSVSPAISPK RSPPVRRR
NT Sequence
ATGCTACGTA TCGCTAAAGA AGCCCTGACG TTTGACGACG TCCTCCTCGT TCCCGCTCAT TCTACCGTTC TGCCGAATAC TGCCGATCTC AGCACTCAGC TGACGAAAAC CATTCGTCTG AATATTCCTA TGCTCTCCGC AGCCATGGAT ACCGTAACGG AAGCGCGTCT GGCTATTGCC CTGGCACAGG AAGGCGGCAT CGGCTTTATT CACAAAAACA TGTCCATCGA GCGCCAGGCG GAAGAAGTTC GTCGCGTGAA GAAACATGAG TCCGGCGTGG TCACCGACCC GCAGACCGTT CTGCCAACCA CCACCCTGCG TGAAGTGAAA GAGCTGACCG AGCGCAACGG TTTCGCCGGC TACCCGGTGG TTACCGAAGA AAACGAGCTG GTTGGCATCA TCACCGGCCG CGACGTGCGT TTCGTCACCG ATCTGAACCA GCCGGTCAGC GTCTACATGA CGCCGAAAGA GCGTCTGGTC ACCGTTCGTG AAGGCGAAAG CCGTGAAGTG GTCTTCGCTA AGATGCACGA AAAACGCGTT GAGAAAGCGC TGGTCGTGGA TGAGAGCTTC CACCTGCGCG GCATGATCAC CGTCAAAGAC TTCCAGAAAG CAGAACGTAA ACCGAACGCC TGTAAAGATG AGCAGGGCCG TCTGCGCGTA GGCGCGGCGG TCGGCGCTGG TGCCGGCAAC GAAGAGCGCG TAGACGCGTT GGTAGCGGCA GGCGTTGACG TTCTGCTGAT CGACTCCTCG CACGGTCACT CAGAAGGCGT TCTGCAGCGT ATCCGTGAAA CGCGCGCGAA ATATCCTGAC CTGCAGATCA TCGGCGGCAA CGTTGCCACT GGCGCAGGCG CACGCGCGCT GGCGGAAGCC GGCTGCAGCG CGGTGAAAGT GGGCATCGGC CCGGGGCTCC ATCTGTACTA CCCCGTATCG TCACCGGCGT GGGTGTGCCG CAGATCACTG CTGTTTCCGA CGCGGTGGAA GCGCTGGAAG GGACCGGTAT TCCGGTTATC GCTGACGGCG GTATCCGTTT CTCCGGCGAT ATCGCCAAAG CGATCGCCGC CGGTGCGGCG GCGG
Details for KlpnC.00062.b.B1.GE44202
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML RIAKEALTFD DVLLVPAHST VLPNTADLST QLTKTIRLNI PMLSAAMDTV TEARLAIALA QEGGIGFIHK NMSIERQAEE VRRVKKHESG VVTDPQTVLP TTTLREVKEL TERNGFAGYP VVTEENELVG IITGRDVRFV TDLNQPVSVY MTPKERLVTV REGESREVVF AKMHEKRVEK ALVVDESFHL RGMITVKDFQ KAERKPNACK DEQGRLRVGA AVGAGAGNEE RVDALVAAGV DVLLIDSSHG HSEGVLQRIR ETRAKYPDLQ IIGGNVATGA GARALAEAGC SAVKVGIGPG SICTTRIVTG VGVPQITAVS DAVEALEGTG IPVIADGGIR FSGDIAK
Validated NT Sequence
gctttggcga tatcgccgga gaaacggata ccgccgtcag cgataaccgg aataccggtc ccttccagcg cttccaccgc gtcggaaaca gcggtgatct gcggcacacc cacgccggtg acgatacggg tagtacagat ggagcccggg ccgatgccca ctttcaccgc gctgcagccg gcttccgcca gcgcgcgtgc gcctgcgcca gtggcgacgt tgccgccgat gatctgcagg tcaggatatt tcgcgcgcgt ttcacggata cgctgcagaa cgccttctga gtgaccgtgc gaggagtcga tcagcagaac gtcaacgcct gccgctacca acgcgtctac gcgctcttcg ttgccggcac cggcgccgac cgccgcgcct acgcgcagac ggccctgctc atctttacag gcgttcggtt tacgttctgc tttctggaag tctttgacgg tgatcatgcc gcgcaggtgg aagctctcat ccacgaccag cgctttctca acgcgttttt cgtgcatctt agcgaagacc acttcacggc tttcgccttc acgaacggtg accagacgct ctttcggcgt catgtagacg ctgaccggct ggttcagatc ggtgacgaaa cgcacgtcgc ggccggtgat gatgccgacc agctcgtttt cttcggtaac caccgggtag ccggcgaaac cgttgcgctc ggtcagctct ttcacttcac gcagagtggt cgtcggcaga acagtctgcg ggtcggtgac cacgccggac tcatgtttct tcacgcgacg aacttcttcc gcctggcgct cgatggacat gtttttgtga ataaagccga tgccgccttc ctgtgccagg gcaatagcca gacgcgcttc cgttacggta tccatggctg cggagagcat aggaatattc agacgaatgg ttttcgtcag ctgagtgctg agatcggcag tattcggcag aacggtagaa tgagcgggaa cgaggaggac gtcgtcaaac gtcagggctt ctttagcgat acgtagcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHML RIAKEALTFD DVLLVPAHST VLPNTADLST QLTKTIRLNI PMLSAAMDTV TEARLAIALA QEGGIGFIHK NMSIERQAEE VRRVKKHESG VVTDPQTVLP TTTLREVKEL TERNGFAGYP VVTEENELVG IITGRDVRFV TDLNQPVSVY MTPKERLVTV REGESREVVF AKMHEKRVEK ALVVDESFHL RGMITVKDFQ KAERKPNACK DEQGRLRVGA AVGAGAGNEE RVDALVAAGV DVLLIDSSHG HSEGVLQRIR ETRAKYPDLQ IIGGNVATGA GARALAEAGC SAVKVGIGPG LHLYYPVSSP AWVCRRSLLF PTRWKRWKGP VFRLSLTAVS VSPAISPKRS PPVRRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctac gtatcgctaa agaagccctg acgtttgacg acgtcctcct cgttcccgct cattctaccg ttctgccgaa tactgccgat ctcagcactc agctgacgaa aaccattcgt ctgaatattc ctatgctctc cgcagccatg gataccgtaa cggaagcgcg tctggctatt gccctggcac aggaaggcgg catcggcttt attcacaaaa acatgtccat cgagcgccag gcggaagaag ttcgtcgcgt gaagaaacat gagtccggcg tggtcaccga cccgcagacc gttctgccaa ccaccaccct gcgtgaagtg aaagagctga ccgagcgcaa cggtttcgcc ggctacccgg tggttaccga agaaaacgag ctggttggca tcatcaccgg ccgcgacgtg cgtttcgtca ccgatctgaa ccagccggtc agcgtctaca tgacgccgaa agagcgtctg gtcaccgttc gtgaaggcga aagccgtgaa gtggtcttcg ctaagatgca cgaaaaacgc gttgagaaag cgctggtcgt ggatgagagc ttccacctgc gcggcatgat caccgtcaaa gacttccaga aagcagaacg taaaccgaac gcctgtaaag atgagcaggg ccgtctgcgc gtaggcgcgg cggtcggcgc tggtgccggc aacgaagagc gcgtagacgc gttggtagcg gcaggcgttg acgttctgct gatcgactcc tcgcacggtc actcagaagg cgttctgcag cgtatccgtg aaacgcgcgc gaaatatcct gacctgcaga tcatcggcgg caacgttgcc actggcgcag gcgcacgcgc gctggcggaa gccggctgca gcgcggtgaa agtgggcatc ggcccggggc tccatctgta ctaccccgta tcgtcaccgg cgtgggtgtg ccgcagatca ctgctgtttc cgacgcggtg gaagcgctgg aagggaccgg tattccggtt atcgctgacg gcggtatccg tttctccggc gatatcgcca aagcgatcgc cgccggtgcg gcggcggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at