SSGCID: Target Page

KlpnC.00056.a

Quinolinate phosphoribosyltransferase [decarboxylating]

CENTER ID:	KlpnC.00056.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00056.a.B1.GE44198	Full length(KlpnC.00056.a)		1	297

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.804
UniProt:	A0A0H3GJC5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSPRRYNPDR RRDVLLERIN LDITDAVAHS LREDLGGEVD ANNDISAQLL PQDARSHAVV ITREDGVFCG KRWVEEVFIQ LAGDDVNITW HVADGDAVKA DQPLFELEGP SRILLTGERT ALNFVQTLSG VASVVRRYVD LLAGTKTQLL DTRKTLPGLR TALKYAVLCG GGANHRLGLF DAFLIKENHI IASGSIRQAV EKAFWLHPDV PVEVEVETLD ELEQALKAGA DIIMLDNFTT DLMREAVKIT AGQAALEVSG NVTFDTIREF ADTGVDYISV GALTKHVQAL DLSMRFR

NT Sequence

ATGTCGCCTC GTCGTTACAA CCCCGACCGC CGCCGTGACG TGCTGCTGGA ACGTATTAAT CTCGATATTA CCGATGCTGT CGCACACTCC CTGCGGGAAG ATCTGGGCGG CGAAGTCGAC GCCAACAACG ATATTAGCGC GCAATTATTG CCGCAGGATG CACGCTCCCA TGCGGTGGTC ATTACTCGTG AAGATGGCGT CTTCTGTGGC AAACGCTGGG TGGAAGAGGT CTTTATTCAG CTCGCCGGTG ACGATGTGAA TATCACCTGG CACGTGGCCG ACGGCGATGC GGTCAAAGCC GACCAGCCGC TGTTCGAATT GGAAGGCCCG TCGCGTATTC TGCTCACCGG CGAACGCACG GCGCTGAACT TTGTGCAAAC CCTCTCCGGC GTCGCCAGCG TCGTCCGTCG CTATGTCGAC CTGCTCGCCG GCACCAAAAC CCAACTGCTG GATACGCGCA AAACGCTGCC AGGGCTGCGT ACCGCGCTGA AATACGCGGT ACTCTGCGGC GGCGGCGCCA ACCATCGACT GGGCCTGTTC GACGCGTTCC TGATTAAAGA AAACCACATT ATCGCCTCCG GATCGATCCG TCAGGCAGTG GAAAAGGCGT TCTGGCTGCA TCCTGATGTG CCGGTGGAAG TGGAAGTGGA AACCCTGGAT GAACTGGAGC AGGCGTTAAA AGCCGGGGCC GATATCATTA TGCTCGATAA CTTCACCACC GACCTGATGC GCGAGGCGGT GAAAATTACC GCAGGCCAGG CGGCGCTGGA GGTCTCCGGC AACGTTACCT TTGACACCAT CCGCGAATTC GCCGATACCG GCGTCGACTA TATTTCCGTC GGCGCCCTGA CCAAACATGT GCAGGCGCTC GACCTGTCGA TGCGCTTCCG C

Details for KlpnC.00056.a.B1.GE44198

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS PRRYNPDRRR DVLLERINLD ITDAVAHSLR EDLGGEVDAN NDISAQLLPQ DARSHAVVIT REDGVFCGKR WVEEVFIQLA GDDVNITWHV ADGDAVKADQ PLFELEGPSR ILLTGERTAL NFVQTLSGVA SVVRRYVDLL AGTKTQLLDT RKTLPGLRTA LKYAVLCGGG ANHRLGLFDA FLIKENHIIA SGSIRQAVEK AFWLHPDVPV EVEVETLDEL EQALKAGADI IMLDNFTTDL MREAVKITAG QAALEVSGNV TFDTIREFAD TGVDYISVGA LTKHVQAXDL SMRFR

Validated NT Sequence

atcttactca cttagcggaa gcgcatcgac aggtcgancg cctgcacatg tttggtcagg gcgccgacgg aaatatagtc gacgccggta tcggcgaatt cgcggatggt gtcaaaggta acgttgccgg agacctccag cgccgcctgg cctgcggtaa ttttcaccgc ctcgcgcatc aggtcggtgg tgaagttatc gagcataatg atatcggccc cggcttttaa cgcctgctcc agttcatcca gggtttccac ttccacttcc accggcacat caggatgcag ccagaacgcc ttttccactg cctgacggat cgatccggag gcgataatgt ggttttcttt aatcaggaac gcgtcgaaca ggcccagtcg atggttggcg ccgccgccgc agagtaccgc gtatttcagc gcggtacgca gccctggcag cgttttgcgc gtatccagca gttgggtttt ggtgccggcg agcaggtcga catagcgacg gacgacgctg gcgacgccgg agagggtttg cacaaagttc agcgccgtgc gttcgccggt gagcagaata cgcgacgggc cttccaattc gaacagcggc tggtcggctt tgaccgcatc gccgtcggcc acgtgccagg tgatattcac atcgtcaccg gcgagctgaa taaagacctc ttccacccag cgtttgccac agaagacgcc atcttcacga gtaatgacca ccgcatggga gcgtgcatcc tgcggcaata attgcgcgct aatatcgttg ttggcgtcga cttcgccgcc cagatcttcc cgcagggagt gtgcgacagc atcggtaata tcgagattaa tacgttccag cagcacgtca cggcggcggt cggggttgta acgacgaggc gacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc ctcgtcgtta caaccccgac cgccgccgtg acgtgctgct ggaacgtatt aatctcgata ttaccgatgc tgtcgcacac tccctgcggg aagatctggg cggcgaagtc gacgccaaca acgatattag cgcgcaatta ttgccgcagg atgcacgctc ccatgcggtg gtcattactc gtgaagatgg cgtcttctgt ggcaaacgct gggtggaaga ggtctttatt cagctcgccg gtgacgatgt gaatatcacc tggcacgtgg ccgacggcga tgcggtcaaa gccgaccagc cgctgttcga attggaaggc ccgtcgcgta ttctgctcac cggcgaacgc acggcgctga actttgtgca aaccctctcc ggcgtcgcca gcgtcgtccg tcgctatgtc gacctgctcg ccggcaccaa aacccaactg ctggatacgc gcaaaacgct gccagggctg cgtaccgcgc tgaaatacgc ggtactctgc ggcggcggcg ccaaccatcg actgggcctg ttcgacgcgt tcctgattaa agaaaaccac attatcgcct ccggatcgat ccgtcaggca gtggaaaagg cgttctggct gcatcctgat gtgccggtgg aagtggaagt ggaaaccctg gatgaactgg agcaggcgtt aaaagccggg gccgatatca ttatgctcga taacttcacc accgacctga tgcgcgaggc ggtgaaaatt accgcaggcc aggcggcgct ggaggtctcc ggcaacgtta cctttgacac catccgcgaa ttcgccgata ccggcgtcga ctatatttcc gtcggcgccc tgaccaaaca tgtgcaggcg ctcgacctgt cgatgcgctt ccgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat