SSGCID: Target Page

KlpnC.00047.a

Fumarate hydratase class II

CENTER ID:	KlpnC.00047.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00047.a.B1.GE44196	Full length(KlpnC.00047.a)		1	466

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00047.a.B1.PS38669	Full length(KlpnC.00047.a)		1	466

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.2354
UniProt:	A0A0H3GWS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTHRSEKDS MGAIEVPADK LWGAQTQRSL EHFRISTEKM PGELIYALAL TKRAAAKVNQ DLGLLTAEKA GAIVAAADEV LAGKHAQEFP LAIWQTGSGT QSNMNMNEVL ANRASELLGG ERGMARKIHP NDDVNKSQSS NDVFPTAMHV AALIALREKV IPSLQALRAT LNEKAVAFRD IVKIGRTHLQ DATPLTLGQE ISGWVAMLDH SLKHIEASQP HLAELALGGT AVGTGLNTHP EYAVRVAAEL ASLSGQPFVT APNKFEALAT VDALVHAHGA LKGLAASLMK IANDVRWLAS GPRCGIGEIA IPENEPGSSI MPGKVNPTQC EALTMVCCQV MGNDVAVNIG GASGNFELNV YRPMVIHNFL QSVRLLADGM ASFNEHCAVG IEPNRERISQ LLNESLMLVT ALNTHIGYDK AAEIAKKAHH EGLTLKASAL ALGYLTEAEF DSWVRPEEMV GSLAIR

NT Sequence

ATGACAACGC ATCGCAGTGA AAAAGACTCG ATGGGCGCCA TCGAGGTCCC GGCCGATAAA CTCTGGGGCG CCCAGACCCA GCGCTCGCTG GAGCATTTCC GCATCTCGAC CGAAAAAATG CCCGGGGAGC TGATATACGC CCTGGCGCTG ACCAAGCGGG CGGCGGCGAA GGTTAACCAG GATCTCGGAC TGTTGACGGC GGAGAAAGCC GGGGCGATCG TCGCCGCCGC TGACGAAGTG CTGGCCGGAA AGCACGCGCA GGAGTTCCCC CTGGCTATCT GGCAGACCGG CTCCGGCACC CAAAGCAATA TGAATATGAA CGAGGTGCTG GCCAACCGCG CCAGTGAACT GCTCGGCGGA GAACGGGGGA TGGCGCGAAA AATTCATCCC AATGACGATG TGAATAAGAG CCAGAGCTCA AATGATGTAT TCCCCACCGC CATGCACGTC GCGGCGCTGA TTGCCCTGCG GGAAAAAGTG ATCCCGTCGC TGCAGGCGCT GCGGGCGACG CTCAATGAAA AAGCGGTGGC CTTCAGGGAT ATCGTCAAGA TCGGCCGCAC CCATCTGCAG GACGCCACGC CGCTCACCTT AGGCCAGGAG ATCTCCGGCT GGGTGGCGAT GCTGGACCAT AGCCTGAAGC ACATTGAAGC CAGTCAGCCG CATCTTGCCG AGCTGGCGCT GGGCGGGACG GCGGTAGGAA CCGGGCTGAA TACCCATCCG GAATATGCCG TACGGGTGGC GGCCGAGCTG GCGTCGCTCA GCGGACAGCC ATTTGTCACC GCGCCGAACA AATTCGAAGC GCTGGCCACT GTGGATGCCC TGGTCCATGC CCACGGCGCG CTGAAAGGCC TGGCGGCCTC GCTGATGAAA ATCGCTAACG ATGTCCGCTG GCTGGCCTCC GGCCCCCGCT GCGGCATTGG TGAAATCGCC ATCCCGGAAA ACGAGCCAGG GTCATCGATT ATGCCGGGCA AGGTTAACCC AACCCAGTGC GAAGCTCTGA CCATGGTCTG CTGCCAGGTG ATGGGCAACG ACGTGGCGGT GAATATCGGC GGTGCTTCCG GCAACTTTGA GCTTAACGTC TATCGCCCGA TGGTCATCCA CAACTTCCTG CAGTCGGTGC GTCTGCTGGC GGACGGTATG GCGAGCTTTA ACGAGCACTG TGCGGTGGGT ATTGAACCGA ATCGCGAGCG GATCAGTCAG TTGCTCAATG AGTCGCTAAT GCTGGTTACT GCGCTGAATA CCCATATCGG CTACGACAAG GCGGCGGAAA TCGCTAAAAA AGCGCACCAT GAGGGGCTGA CTCTCAAAGC CTCGGCGCTG GCGCTGGGCT ACCTGACGGA AGCCGAATTC GACAGCTGGG TGCGCCCGGA AGAGATGGTC GGCAGCCTGG CTATTCGC

Details for KlpnC.00047.a.B1.GE44196

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT THRSEKDSMG AIEVPADKLW GAQTQRSLEH FRISTEKMPG ELIYALALTK RAAAKVNQDL GLLTAEKAGA IXXAADEVLA GKHAQEFPLA IWQTGSGTQS NMNMNEVLAN RAXELLGGER GMARKIXPND DVNXXXSSND VFPTAMHVXA LIXLREXXIP SLQALRATLN EKXVXFXDIX KIGRTHLQDA TPLXXXQEIS GWVAMLDHXL KHIEASQPHL AELALGGTAV GTGLNTHPEY AVRVAAELAS LSGQPFVTAP NKFEALATVD ALVHAHGALK GLAASLMKIA NDVRWLASGP RCGIGEIAIP ENEPGSSIMP GKVNPTQCEA LTMVCCQVMG NDVAVNIGGA SGNFELNVYR PMVIHNFLQS VRLLADGMAS FNEHCAVGIE PNRERISQLL NESLMLVTAL NTHIGYDKAA EIAKKAHHEG LTLKASALAL GYLTEAEFDS WVRPEEX

Validated NT Sequence

annntctctt ccgggcgcac ccagctgtcg aattcggctt ccgtcaggta gcccagcgcc agcgccgagg ctttgagagt cagcccctca tggtgcgctt ttttagcgat ttccgccgcc ttgtcgtagc cgatatgggt attcagcgca gtaaccagca tcagcgactc attgagcaac tgactgatcc gctcgcgatt cggttcaata cccaccgcac agtgctcgtt aaagctcgcc ataccgtccg ccagcagacg caccgactgc aggaagttgt ggatgaccat cgggcgatag acgttaagct caaagttgcc ggaagcaccg ccgatattca ccgccacgtc gttgcccatc acctggcagc agaccatggt cagagcttcg cactgggttg ggttaacctt gcccggcata atcgatgacc ctggctcgtt ttccgggatg gcgatttcac caatgccgca gcgggggccg gaggccagcc agcggacatc gttagcgatt ttcatcagcg aggccgccag gcctttcagc gcgccgtggg catggaccag ggcatccaca gtggccagcg cttcgaattt gttcggcgcg gtgacaaatg gctgtccgct gagcgacgcc agctcggccg ccacccgtac ggcatattcc ggatgggtat tcagcccggt tcctaccgcc gtcccgccca gcgccagctc ggcaagatgc ggctgactgg cttcaatgtg cttcagnnta tggtccagca tcgccaccca gccggagatc tcctgnncna agnngagcgg cgtggcgtcc tgcagatggg tgcggccgat cttgangata tccntgaagn ccaccgnttt ttcattgagc gtcgcccgca gcgcctgcag cgacgggatc nnnttttccc gcaggncaat cagcgccgng acgtgcatgg cggtggggaa tacatcattn gagctntgnn tnttattcac atcgtcattg ggannaattt ttcgcgccat cccccgttct ccgccgagca gttcnnnggc gcggttggcc agcacctcgt tcatattcat attgctttgg gtgccggagc cggtctgcca gatagccagg gggaactcct gcgcgtgctt tccggccagc acttcgtcag cggcggngnc gatcgccccg gctttctccg ccgtcaacag tccgagatcc tggttaacct tcgccgccgc ccgcttggtc agcgccaggg cgtatatcag ctccccgggc attttttcgg tcgagatgcg gaaatgctcc agcgagcgct gggtctgggc gccccagagt ttatcggccg ggacctcgat ggcgcccatc gagtcttttt cactgcgatg cgttgtcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMT THRSEKDSMG AIEVPADKLW GAQTQRSLEH FRISTEKMPG ELIYALALTK RAAAKVNQDL GLLTAEKAGA IVAAADEVLA GKHAQEFPLA IWQTGSGTQS NMNMNEVLAN RASELLGGER GMARKIHPND DVNKSQSSND VFPTAMHVAA LIALREKVIP SLQALRATLN EKAVAFRDIV KIGRTHLQDA TPLTLGQEIS GWVAMLDHSL KHIEASQPHL AELALGGTAV GTGLNTHPEY AVRVAAELAS LSGQPFVTAP NKFEALATVD ALVHAHGALK GLAASLMKIA NDVRWLASGP RCGIGEIAIP ENEPGSSIMP GKVNPTQCEA LTMVCCQVMG NDVAVNIGGA SGNFELNVYR PMVIHNFLQS VRLLADGMAS FNEHCAVGIE PNRERISQLL NESLMLVTAL NTHIGYDKAA EIAKKAHHEG LTLKASALAL GYLTEAEFDS WVRPEEMVGS LAIR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa cgcatcgcag tgaaaaagac tcgatgggcg ccatcgaggt cccggccgat aaactctggg gcgcccagac ccagcgctcg ctggagcatt tccgcatctc gaccgaaaaa atgcccgggg agctgatata cgccctggcg ctgaccaagc gggcggcggc gaaggttaac caggatctcg gactgttgac ggcggagaaa gccggggcga tcgtcgccgc cgctgacgaa gtgctggccg gaaagcacgc gcaggagttc cccctggcta tctggcagac cggctccggc acccaaagca atatgaatat gaacgaggtg ctggccaacc gcgccagtga actgctcggc ggagaacggg ggatggcgcg aaaaattcat cccaatgacg atgtgaataa gagccagagc tcaaatgatg tattccccac cgccatgcac gtcgcggcgc tgattgccct gcgggaaaaa gtgatcccgt cgctgcaggc gctgcgggcg acgctcaatg aaaaagcggt ggccttcagg gatatcgtca agatcggccg cacccatctg caggacgcca cgccgctcac cttaggccag gagatctccg gctgggtggc gatgctggac catagcctga agcacattga agccagtcag ccgcatcttg ccgagctggc gctgggcggg acggcggtag gaaccgggct gaatacccat ccggaatatg ccgtacgggt ggcggccgag ctggcgtcgc tcagcggaca gccatttgtc accgcgccga acaaattcga agcgctggcc actgtggatg ccctggtcca tgcccacggc gcgctgaaag gcctggcggc ctcgctgatg aaaatcgcta acgatgtccg ctggctggcc tccggccccc gctgcggcat tggtgaaatc gccatcccgg aaaacgagcc agggtcatcg attatgccgg gcaaggttaa cccaacccag tgcgaagctc tgaccatggt ctgctgccag gtgatgggca acgacgtggc ggtgaatatc ggcggtgctt ccggcaactt tgagcttaac gtctatcgcc cgatggtcat ccacaacttc ctgcagtcgg tgcgtctgct ggcggacggt atggcgagct ttaacgagca ctgtgcggtg ggtattgaac cgaatcgcga gcggatcagt cagttgctca atgagtcgct aatgctggtt actgcgctga atacccatat cggctacgac aaggcggcgg aaatcgctaa aaaagcgcac catgaggggc tgactctcaa agcctcggcg ctggcgctgg gctacctgac ggaagccgaa ttcgacagct gggtgcgccc ggaagagatg gtcggcagcc tggctattcg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for KlpnC.00047.a.B1.PS38669

PURIFICATION DATe:	4/26/2021
CONCENTRATION:	30.744mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA CGCATCGCAG TGAAAAAGAC TCGATGGGCG CCATCGAGGT CCCGGCCGAT AAACTCTGGG GCGCCCAGAC CCAGCGCTCG CTGGAGCATT TCCGCATCTC GACCGAAAAA ATGCCCGGGG AGCTGATATA CGCCCTGGCG CTGACCAAGC GGGCGGCGGC GAAGGTTAAC CAGGATCTCG GACTGTTGAC GGCGGAGAAA GCCGGGGCGA TCGTCGCCGC CGCTGACGAA GTGCTGGCCG GAAAGCACGC GCAGGAGTTC CCCCTGGCTA TCTGGCAGAC CGGCTCCGGC ACCCAAAGCA ATATGAATAT GAACGAGGTG CTGGCCAACC GCGCCAGTGA ACTGCTCGGC GGAGAACGGG GGATGGCGCG AAAAATTCAT CCCAATGACG ATGTGAATAA GAGCCAGAGC TCAAATGATG TATTCCCCAC CGCCATGCAC GTCGCGGCGC TGATTGCCCT GCGGGAAAAA GTGATCCCGT CGCTGCAGGC GCTGCGGGCG ACGCTCAATG AAAAAGCGGT GGCCTTCAGG GATATCGTCA AGATCGGCCG CACCCATCTG CAGGACGCCA CGCCGCTCAC CTTAGGCCAG GAGATCTCCG GCTGGGTGGC GATGCTGGAC CATAGCCTGA AGCACATTGA AGCCAGTCAG CCGCATCTTG CCGAGCTGGC GCTGGGCGGG ACGGCGGTAG GAACCGGGCT GAATACCCAT CCGGAATATG CCGTACGGGT GGCGGCCGAG CTGGCGTCGC TCAGCGGACA GCCATTTGTC ACCGCGCCGA ACAAATTCGA AGCGCTGGCC ACTGTGGATG CCCTGGTCCA TGCCCACGGC GCGCTGAAAG GCCTGGCGGC CTCGCTGATG AAAATCGCTA ACGATGTCCG CTGGCTGGCC TCCGGCCCCC GCTGCGGCAT TGGTGAAATC GCCATCCCGG AAAACGAGCC AGGGTCATCG ATTATGCCGG GCAAGGTTAA CCCAACCCAG TGCGAAGCTC TGACCATGGT CTGCTGCCAG GTGATGGGCA ACGACGTGGC GGTGAATATC GGCGGTGCTT CCGGCAACTT TGAGCTTAAC GTCTATCGCC CGATGGTCAT CCACAACTTC CTGCAGTCGG TGCGTCTGCT GGCGGACGGT ATGGCGAGCT TTAACGAGCA CTGTGCGGTG GGTATTGAAC CGAATCGCGA GCGGATCAGT CAGTTGCTCA ATGAGTCGCT AATGCTGGTT ACTGCGCTGA ATACCCATAT CGGCTACGAC AAGGCGGCGG AAATCGCTAA AAAAGCGCAC CATGAGGGGC TGACTCTCAA AGCCTCGGCG CTGGCGCTGG GCTACCTGAC GGAAGCCGAA TTCGACAGCT GGGTGCGCCC GGAAGAGATG GTCGGCAGCC TGGCTATTCG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT