SSGCID: Target Page

KlpnC.00021.a

Erythronate-4-phosphate dehydrogenase

CENTER ID:	KlpnC.00021.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00021.a.B1.GE44192	Full length(KlpnC.00021.a)		1	378

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.00021.a.B1.PW38977	Full length(KlpnC.00021.a)		1	378

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.3683
UniProt:	A0A0H3GVT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKILVDENMP YARELFSRLG DVQAIPGRPV PADALTDADA LMVRSVTRVN EALLAGKAIK FVGTATAGTD HVDQAWLQQA GIGFSAAPGC NAIAVVEYVF SSLLMLAERD GFALRDRTVG IVGVGNVGGR LQKRLEALGI KTLLCDPPRA DRGDEGDFRS LEALVQEADV ITFHTPLYKE GQYKTLHLAD EALISRLKPG TILINACRGP VVDNAALLKR LEAGQPLSVV LDVWEPEPDL NVELLKRVDI GTAHIAGYTL EGKARGTTQV FEAYSAFIGH PQQVALDTLL PAPEFGRITL HGPLDQPTLK RLVHLVYDVR RDDAPLRKVA GVAGEFDKLR KNYQERREWS SLYVQCSDEQ AATLLRQLGF NAVHHPVR

NT Sequence

GTGAAAATCC TCGTTGATGA AAATATGCCC TATGCCCGTG AGCTTTTTAG CCGTTTAGGC GACGTGCAGG CGATCCCAGG CCGTCCGGTC CCGGCCGATG CCCTGACCGA TGCGGATGCC CTAATGGTGC GCTCGGTCAC CCGGGTCAAT GAAGCATTGC TCGCCGGCAA GGCCATTAAG TTCGTCGGCA CCGCTACCGC CGGCACCGAT CACGTCGATC AGGCGTGGCT GCAGCAGGCG GGGATTGGTT TCTCTGCGGC GCCAGGCTGT AACGCCATTG CGGTGGTAGA GTACGTCTTT TCATCGCTGC TGATGCTGGC TGAACGCGAC GGCTTTGCGC TGCGCGATCG CACCGTCGGG ATCGTCGGCG TCGGTAACGT TGGCGGGCGA CTGCAAAAGC GCCTGGAAGC GTTGGGGATC AAAACTCTGC TCTGCGACCC ACCGCGCGCC GATCGCGGCG ACGAAGGTGA CTTCCGCTCC CTCGAGGCGC TGGTCCAGGA AGCCGATGTC ATCACCTTCC ATACGCCGCT GTATAAAGAA GGGCAATATA AAACCCTGCA TCTGGCGGAT GAGGCGCTGA TAAGCCGCCT GAAGCCTGGA ACGATTTTGA TCAACGCCTG CCGCGGGCCG GTGGTCGATA ACGCCGCACT GCTAAAGCGG CTGGAAGCTG GTCAGCCGCT GAGTGTGGTA TTGGACGTCT GGGAGCCGGA GCCGGATCTA AACGTTGAGC TTCTCAAGCG AGTGGATATC GGTACCGCGC ATATCGCCGG CTACACCCTC GAAGGGAAAG CGCGCGGGAC GACGCAGGTT TTTGAAGCCT ACAGCGCCTT TATCGGCCAT CCGCAGCAGG TAGCGCTGGA CACTCTGCTG CCGGCGCCGG AATTTGGCCG CATTACCCTG CATGGACCGC TCGATCAGCC GACGCTGAAA AGACTGGTCC ACTTGGTGTA TGATGTGCGC CGCGATGATG CGCCGCTGCG GAAGGTGGCC GGCGTCGCCG GTGAATTCGA CAAGCTGCGT AAAAATTATC AGGAACGTCG CGAATGGTCT TCGCTGTATG TGCAGTGCAG CGATGAGCAG GCGGCGACGC TGTTGCGCCA GCTCGGTTTT AACGCCGTGC ATCATCCTGT TCGT

Details for KlpnC.00021.a.B1.GE44192

HARVESTED ON:	4/1/2021
SEQUENCED ON:	4/13/2021
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMV KILVDENMPY ARELFSRLGD VQAIPGRPVP ADALTDADAL MVRSVTRVNE ALLAGKAIKF VGTATAGTDH VDQAWLQQAG IGFSAAPGCN AIAVVEYVFS SLLMLAERDG FALRDRTVGI VGVGNVGGRL QKRLEALGIK TLLCDPPRAD RGDEGDFRSL EALVQEADVI TFHTPLYKEG QYKTLHLADE ALISRLKPGT ILINACRGPV VDNAALLKRL EAGQPLSVVL DVWEPEPDLN VELLKRVDIG TAHIAGYTLE GKARGTTQVF EAYSAFIGHP QQVALDTLLP APEFGRITLH GPLDQPTLKR LVHLVYDVRR DDAPLRKVAG VAGEFDKLRK NYQERREWSS LYVQCSDEQA ATLLRQLG

Validated NT Sequence

aaaccgagct ggcgcaacag cgtcgccgcc tgctcatcgc tgcactgcac atacagcgaa gaccattcgc gacgttcctg ataattttta cgcagcttgt cgaattcacc ggcgacgccg gccaccttcc gcagcggcgc atcatcgcgg cgcacatcat acaccaagtg gaccagtctt ttcagcgtcg gctgatcgag cggtccatgc agggtaatgc ggccaaattc cggcgccggc agcagagtgt ccagcgctac ctgctgcgga tggccgataa aggcgctgta ggcttcaaaa acctgcgtcg tcccgcgcgc tttcccttcg agggtgtagc cggcgatatg cgcggtaccg atatccactc gcttgagaag ctcaacgttt agatccggct ccggctccca gacgtccaat accacactca gcggctgacc agcttccagc cgctttagca gtgcggcgtt atcgaccacc ggcccgcggc aggcgttgat caaaatcgtt ccaggcttca ggcggcttat cagcgcctca tccgccagat gcagggtttt atattgccct tctttataca gcggcgtatg gaaggtgatg acatcggctt cctggaccag cgcctcgagg gagcggaagt caccttcgtc gccgcgatcg gcgcgcggtg ggtcgcagag cagagttttg atccccaacg cttccaggcg cttttgcagt cgcccgccaa cgttaccgac gccgacgatc ccgacggtgc gatcgcgcag cgcaaagccg tcgcgttcag ccagcatcag cagcgatgaa aagacgtact ctaccaccgc aatggcgtta cagccaggcg ccgcagagaa accaatcccc gcctgctgca gccacgcctg atcgacgtga tcggtgccgg cggtagcggt gccgacgaac ttaatggcct tgccggcgag caatgcttca ttgacccggg tgaccgagcg caccattagg gcatccgcat cggtcagggc atcggccggg accggacggc ctgggatcgc ctgcacgtcg cctaaacggc taaaaagctc acgggcatag ggcatatttt catcaacgag gattttcacc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMK ILVDENMPYA RELFSRLGDV QAIPGRPVPA DALTDADALM VRSVTRVNEA LLAGKAIKFV GTATAGTDHV DQAWLQQAGI GFSAAPGCNA IAVVEYVFSS LLMLAERDGF ALRDRTVGIV GVGNVGGRLQ KRLEALGIKT LLCDPPRADR GDEGDFRSLE ALVQEADVIT FHTPLYKEGQ YKTLHLADEA LISRLKPGTI LINACRGPVV DNAALLKRLE AGQPLSVVLD VWEPEPDLNV ELLKRVDIGT AHIAGYTLEG KARGTTQVFE AYSAFIGHPQ QVALDTLLPA PEFGRITLHG PLDQPTLKRL VHLVYDVRRD DAPLRKVAGV AGEFDKLRKN YQERREWSSL YVQCSDEQAA TLLRQLGFNA VHHPVR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaaa tcctcgttga tgaaaatatg ccctatgccc gtgagctttt tagccgttta ggcgacgtgc aggcgatccc aggccgtccg gtcccggccg atgccctgac cgatgcggat gccctaatgg tgcgctcggt cacccgggtc aatgaagcat tgctcgccgg caaggccatt aagttcgtcg gcaccgctac cgccggcacc gatcacgtcg atcaggcgtg gctgcagcag gcggggattg gtttctctgc ggcgccaggc tgtaacgcca ttgcggtggt agagtacgtc ttttcatcgc tgctgatgct ggctgaacgc gacggctttg cgctgcgcga tcgcaccgtc gggatcgtcg gcgtcggtaa cgttggcggg cgactgcaaa agcgcctgga agcgttgggg atcaaaactc tgctctgcga cccaccgcgc gccgatcgcg gcgacgaagg tgacttccgc tccctcgagg cgctggtcca ggaagccgat gtcatcacct tccatacgcc gctgtataaa gaagggcaat ataaaaccct gcatctggcg gatgaggcgc tgataagccg cctgaagcct ggaacgattt tgatcaacgc ctgccgcggg ccggtggtcg ataacgccgc actgctaaag cggctggaag ctggtcagcc gctgagtgtg gtattggacg tctgggagcc ggagccggat ctaaacgttg agcttctcaa gcgagtggat atcggtaccg cgcatatcgc cggctacacc ctcgaaggga aagcgcgcgg gacgacgcag gtttttgaag cctacagcgc ctttatcggc catccgcagc aggtagcgct ggacactctg ctgccggcgc cggaatttgg ccgcattacc ctgcatggac cgctcgatca gccgacgctg aaaagactgg tccacttggt gtatgatgtg cgccgcgatg atgcgccgct gcggaaggtg gccggcgtcg ccggtgaatt cgacaagctg cgtaaaaatt atcaggaacg tcgcgaatgg tcttcgctgt atgtgcagtg cagcgatgag caggcggcga cgctgttgcg ccagctcggt tttaacgccg tgcatcatcc tgttcgttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for KlpnC.00021.a.B1.PW38977

PURIFICATION DATe:	4/19/2021
CONCENTRATION:	41mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAAAA TCCTCGTTGA TGAAAATATG CCCTATGCCC GTGAGCTTTT TAGCCGTTTA GGCGACGTGC AGGCGATCCC AGGCCGTCCG GTCCCGGCCG ATGCCCTGAC CGATGCGGAT GCCCTAATGG TGCGCTCGGT CACCCGGGTC AATGAAGCAT TGCTCGCCGG CAAGGCCATT AAGTTCGTCG GCACCGCTAC CGCCGGCACC GATCACGTCG ATCAGGCGTG GCTGCAGCAG GCGGGGATTG GTTTCTCTGC GGCGCCAGGC TGTAACGCCA TTGCGGTGGT AGAGTACGTC TTTTCATCGC TGCTGATGCT GGCTGAACGC GACGGCTTTG CGCTGCGCGA TCGCACCGTC GGGATCGTCG GCGTCGGTAA CGTTGGCGGG CGACTGCAAA AGCGCCTGGA AGCGTTGGGG ATCAAAACTC TGCTCTGCGA CCCACCGCGC GCCGATCGCG GCGACGAAGG TGACTTCCGC TCCCTCGAGG CGCTGGTCCA GGAAGCCGAT GTCATCACCT TCCATACGCC GCTGTATAAA GAAGGGCAAT ATAAAACCCT GCATCTGGCG GATGAGGCGC TGATAAGCCG CCTGAAGCCT GGAACGATTT TGATCAACGC CTGCCGCGGG CCGGTGGTCG ATAACGCCGC ACTGCTAAAG CGGCTGGAAG CTGGTCAGCC GCTGAGTGTG GTATTGGACG TCTGGGAGCC GGAGCCGGAT CTAAACGTTG AGCTTCTCAA GCGAGTGGAT ATCGGTACCG CGCATATCGC CGGCTACACC CTCGAAGGGA AAGCGCGCGG GACGACGCAG GTTTTTGAAG CCTACAGCGC CTTTATCGGC CATCCGCAGC AGGTAGCGCT GGACACTCTG CTGCCGGCGC CGGAATTTGG CCGCATTACC CTGCATGGAC CGCTCGATCA GCCGACGCTG AAAAGACTGG TCCACTTGGT GTATGATGTG CGCCGCGATG ATGCGCCGCT GCGGAAGGTG GCCGGCGTCG CCGGTGAATT CGACAAGCTG CGTAAAAATT ATCAGGAACG TCGCGAATGG TCTTCGCTGT ATGTGCAGTG CAGCGATGAG CAGGCGGCGA CGCTGTTGCG CCAGCTCGGT TTTAACGCCG TGCATCATCC TGTTCGTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT