KlpnC.00020.d

AldA, aldehyde dehydrogenase A

CENTER ID: KlpnC.00020.d
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.00020.d.B1.GE44191 Full length(KlpnC.00020.d) 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.2338
UniProt: A0A0H3GP48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFKRALGVT TGILPWNFPF FLIARKLAPA LITGNTIVIK PSEFTPNNAI AFAEIVHQVG LPKGVFNLVL GRGETVGQEL AGNPKVAMVS MTGSVAAGEK IMAAAAKNIT KVCLELGGKA PAIVMDDADL ELAVKAVVDS RVINTGQVCN CVERVYVQQG IYDRFVNRLG EAMKAVQFGD PATRDDIAMG PLINAAARDQ VAGKVAKAVA QGARVALGGQ PLEGKGYFYP PTLLLDVRQE MDIIHEETFG PVLPVVAFST LDEALAMAND SDYGLTSSIY TRDLNVAMKA IKGLKFGETY INRENFEAMQ GFHAGWRKSG IGGADGRHGL NEYLQTQVVY LQA
NT Sequence
TTGTCTTTTA AACGCGCGCT GGGGGTGACC ACCGGGATCC TGCCGTGGAA CTTCCCGTTC TTTCTTATCG CCCGCAAGCT GGCGCCGGCT CTCATCACTG GAAATACCAT TGTCATTAAG CCCAGCGAAT TTACGCCCAA TAATGCCATC GCCTTTGCCG AGATTGTCCA TCAGGTTGGG TTGCCGAAAG GGGTCTTTAA CCTTGTGCTT GGCCGCGGAG AAACCGTTGG CCAGGAGCTG GCCGGCAATC CGAAGGTGGC GATGGTCAGC ATGACCGGCA GCGTGGCGGC GGGAGAAAAA ATTATGGCCG CTGCGGCGAA AAATATCACC AAAGTGTGCC TCGAGCTCGG CGGCAAAGCG CCTGCCATTG TGATGGACGA TGCGGATCTG GAGCTGGCGG TGAAAGCGGT GGTGGACTCG CGGGTGATTA ACACCGGGCA GGTGTGTAAC TGCGTCGAGC GGGTCTATGT TCAGCAGGGC ATTTACGACC GCTTCGTCAA CCGCCTCGGC GAGGCGATGA AGGCCGTGCA GTTTGGCGAC CCGGCGACGC GGGATGACAT CGCGATGGGG CCGCTGATCA ACGCGGCGGC GCGGGACCAG GTGGCGGGCA AAGTGGCGAA GGCGGTGGCG CAGGGGGCGC GGGTGGCGCT GGGCGGTCAG CCGCTGGAGG GCAAAGGCTA TTTTTATCCG CCGACCCTGC TGCTGGATGT ACGTCAGGAG ATGGACATTA TCCATGAGGA AACCTTCGGT CCGGTGCTGC CGGTGGTGGC CTTTTCGACC CTCGATGAGG CGCTGGCGAT GGCCAATGAC AGCGATTATG GCCTGACCTC CTCAATCTAT ACCCGCGATC TGAACGTGGC GATGAAAGCG ATTAAGGGAC TGAAGTTTGG CGAAACCTAC ATCAACCGTG AGAACTTTGA GGCGATGCAG GGGTTCCACG CCGGCTGGCG TAAGTCGGGG ATCGGCGGGG CAGATGGCCG CCACGGCCTG AATGAGTACC TGCAGACCCA GGTGGTCTAT CTGCAGGCC
Details for KlpnC.00020.d.B1.GE44191
HARVESTED ON: 4/1/2021
SEQUENCED ON: 4/13/2021
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML SFKRALGVTT GILPWNFPFF LIARKLAPAL ITGNTIVIKP SEFTPNNAIA FAEIVHQVGL PKGVFNLVLG RGETVGQELA GNPKVAMVSM TGSVAAGEKI MAAAAKNITK VCLELGGKAP AIVMDDADLE LAVKAVVDSR VINTGQVCNC VERVYVQQGI YDRFVNRLGE AMKAVQFGDP ATRDDIAMGP LINAAARDQV AGKVAKAVAQ GARVALGGQP LEGKGYFYPP TLLLDVRQGM DIIHEETFGP VLPVVAFSTL DEALAMANDS DYGLTSSIYT RDLNVAMKAI KGLKFGETYI NRENFEAMQG FHAGWRKSGI GGADGRHGLN EYL
Validated NT Sequence
ctgcaggtac tcattcaggc cgtggcggcc atctgcgccg ccgatccccg acttacgcca gccggcgtgg aacccctgca tcgcctcaaa gttctcacgg ttgatatagg tttcgccaaa cttcagtccc ttaatcgctt tcatcgccac gttcagatcg cgggtataga ttgaggaggt caggccataa tcgctgtcat tggccatcgc cagcgcctca tcgagggtcg aaaaggccac caccggcagc accggaccga aggtttcctc atggataatg tccatcccct gacgtacatc cagcagaagg gtcggcggat aaaaatagcc tttgccctcc agcggctgac cgcccagcgc cacccgcgcc ccctgcgcca ccgccttcgc cactttgccc gccacctggt cccgcgccgc cgcgttgatc agcggcccca tcgcgatgtc atcccgcgtc gccgggtcgc caaactggac ggccttcatc gcctcaccga ggcggttgac gaagcggtcg tatattccct gctgaacata gacccgctcg acgcagttac acacctgccc ggtgttaatc acccgcgagt ccactaccgc tttcaccgcc agctccagat ccgcatcgtc cattacaatg gcaggcgctt tgccgccgag ctcgaggcac actttggtga tatttttcgc cgcagcggcc ataatttttt ctcccgccgc cacgctgccg gtcatgctga ccatcgccac cttcggattg ccggccagct cctggccaac ggtttctccg cggccaagca caaggttaaa gacccctttc ggcaacccaa cctgatggac aatctcggca aaggcgatgg cattattggg cgtaaattcg ctgggcttaa tgacgatggt attcccggtg atcagggccg gcgccagctt gcgggcgata agaaagaacg ggaagttcca cggcaggatc ccggtggtca cccccagcgc gcgtttaaaa gacaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMS FKRALGVTTG ILPWNFPFFL IARKLAPALI TGNTIVIKPS EFTPNNAIAF AEIVHQVGLP KGVFNLVLGR GETVGQELAG NPKVAMVSMT GSVAAGEKIM AAAAKNITKV CLELGGKAPA IVMDDADLEL AVKAVVDSRV INTGQVCNCV ERVYVQQGIY DRFVNRLGEA MKAVQFGDPA TRDDIAMGPL INAAARDQVA GKVAKAVAQG ARVALGGQPL EGKGYFYPPT LLLDVRQEMD IIHEETFGPV LPVVAFSTLD EALAMANDSD YGLTSSIYTR DLNVAMKAIK GLKFGETYIN RENFEAMQGF HAGWRKSGIG GADGRHGLNE YLQTQVVYLQ A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtctt ttaaacgcgc gctgggggtg accaccggga tcctgccgtg gaacttcccg ttctttctta tcgcccgcaa gctggcgccg gctctcatca ctggaaatac cattgtcatt aagcccagcg aatttacgcc caataatgcc atcgcctttg ccgagattgt ccatcaggtt gggttgccga aaggggtctt taaccttgtg cttggccgcg gagaaaccgt tggccaggag ctggccggca atccgaaggt ggcgatggtc agcatgaccg gcagcgtggc ggcgggagaa aaaattatgg ccgctgcggc gaaaaatatc accaaagtgt gcctcgagct cggcggcaaa gcgcctgcca ttgtgatgga cgatgcggat ctggagctgg cggtgaaagc ggtggtggac tcgcgggtga ttaacaccgg gcaggtgtgt aactgcgtcg agcgggtcta tgttcagcag ggcatttacg accgcttcgt caaccgcctc ggcgaggcga tgaaggccgt gcagtttggc gacccggcga cgcgggatga catcgcgatg gggccgctga tcaacgcggc ggcgcgggac caggtggcgg gcaaagtggc gaaggcggtg gcgcaggggg cgcgggtggc gctgggcggt cagccgctgg agggcaaagg ctatttttat ccgccgaccc tgctgctgga tgtacgtcag gagatggaca ttatccatga ggaaaccttc ggtccggtgc tgccggtggt ggccttttcg accctcgatg aggcgctggc gatggccaat gacagcgatt atggcctgac ctcctcaatc tatacccgcg atctgaacgt ggcgatgaaa gcgattaagg gactgaagtt tggcgaaacc tacatcaacc gtgagaactt tgaggcgatg caggggttcc acgccggctg gcgtaagtcg gggatcggcg gggcagatgg ccgccacggc ctgaatgagt acctgcagac ccaggtggtc tatctgcagg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat