KlpnA.00166.a

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (LpxC)

CENTER ID: KlpnA.00166.a
ORGANISM: Klebsiella pneumoniae
ASSOCIATED DISEASE: Pneumonia
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnA.00166.a.AE1.GE44058 full length 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272620.9.peg.103
RefSeq: WP_002888632.1
UniProt: R4Y5T7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT MLCTCLVNEH DVRISTVEHL NAALAGLGID NIIVEVDAPE IPIMDGSAAP FVYLLLDAGI DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDAST QRYTLNFSAD AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFEDD AKLPMAFRAP SMVLA
NT Sequence
atgatcaaac aaaggactct taaacgtatc gttcaggcga ctggcgtcgg tttgcatacc ggcaagaaag tcactctgac gttacgccct gcgccggcaa ataccggggt catctatcgt cgcaccgact tgaatccacc ggtagatttt ccggctgatg ccaaatctgt gcgtgatacc atgctctgta cttgcctggt taatgagcat gacgtgcgga tttctacggt tgaacacctg aacgccgcat tggctggcct gggtatcgac aacattattg ttgaagtcga tgcgccggaa atcccgatta tggacggcag tgcggctccg ttcgtttatc tgttgctgga cgccggcatt gatgaactga actgcgccaa gaaatttgta cgtatcaaag agacggttcg cgtcgaagat ggcgacaaat gggctgaatt caaaccgtac aatggttttt cgctggactt caccatcgat tttaaccatc cagcgattga tgccagcacc cagcgctata cgctgaactt ctcggccgat gcgttcatgc gtcagattag ccgtgcgcgt accttcggtt ttatgcgcga tatcgaatat ctgcagtccc gcggcctgtg cctgggcggc agcttcgatt gtgccatcgt tgttgacgat tatcgcgtac tgaacgaaga cggtctgcgc tttgaagacg aatttgttcg ccacaaaatg ctggatgcga tcggtgacct gtttatgtgt ggtcacaaca ttatcggcgc attcacggcg tacaaatcgg gtcacgcgtt gaacaacaaa ctgctgcagg cggttctggc aaaacaggaa gcctgggagt atgtgacctt cgaagacgac gcaaaactgc caatggcgtt ccgcgcaccg tcgatggttc tggcgtaa
Details for KlpnA.00166.a.AE1.GE44058
HARVESTED ON: 1/7/2021
SEQUENCED ON: 1/8/2021
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT MLCTCLVNEH DVRISTVEHL NAALAGLGID NIIVEVDAPE IPIMDGSAAP FVYLLLDAGI DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDAST QRYTLNFSAD AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFEDD AKLPMAFRAP SMVLAAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ccgccagaac catcgacggt gcgcggaacg ccattggcag ttttgcgtcg tcttcgaagg tcacatactc ccaggcttcc tgttttgcca gaaccgcctg cagcagtttg ttgttcaacg cgtgacccga tttgtacgcc gtgaatgcgc cgataatgtt gtgaccacac ataaacaggt caccgatcgc atccagcatt ttgtggcgaa caaattcgtc ttcaaagcgc agaccgtctt cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca ggccgcggga ctgcagatat tcgatatcgc gcataaaacc gaaggtacgc gcacggctaa tctgacgcat gaacgcatcg gccgagaagt tcagggtata gcgctgggtg ctggcatcaa tcgctggatg gttaaaatcg atggtgaagt ccagcgaaaa accattgtac ggtttgaatt cagcccattt gtcgccatct tcgacgcgaa ccgtctcttt gatacgtaca aatttcttgg cgcagttcag ttcatcaatg ccggcgtcca gcaacagata aacgaacgga gccgcactgc cgtccataat cgggatttcc ggcgcatcga cttcaacaat aatgttgtcg atacccaggc cagccaatgc ggcgttcagg tgttcaaccg tagaaatccg cacgtcatgc tcattaacca ggcaagtaca gagcatggta tcacgcacag atttggcatc agccggaaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc cggtatttgc cggcgcaggg cgtaacgtca gtgtgacttt cttgccggta tgcaaaccga cgccagtcgc ctgaacgata cgtttaagag tcctttgttt gatcatggga gaga
Expected Protein Sequence
MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT MLCTCLVNEH DVRISTVEHL NAALAGLGID NIIVEVDAPE IPIMDGSAAP FVYLLLDAGI DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDAST QRYTLNFSAD AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFEDD AKLPMAFRAP SMVLAGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gatcaaacaa aggactctta aacgtatcgt tcaggcgact ggcgtcggtt tgcataccgg caagaaagtc actctgacgt tacgccctgc gccggcaaat accggggtca tctatcgtcg caccgacttg aatccaccgg tagattttcc ggctgatgcc aaatctgtgc gtgataccat gctctgtact tgcctggtta atgagcatga cgtgcggatt tctacggttg aacacctgaa cgccgcattg gctggcctgg gtatcgacaa cattattgtt gaagtcgatg cgccggaaat cccgattatg gacggcagtg cggctccgtt cgtttatctg ttgctggacg ccggcattga tgaactgaac tgcgccaaga aatttgtacg tatcaaagag acggttcgcg tcgaagatgg cgacaaatgg gctgaattca aaccgtacaa tggtttttcg ctggacttca ccatcgattt taaccatcca gcgattgatg ccagcaccca gcgctatacg ctgaacttct cggccgatgc gttcatgcgt cagattagcc gtgcgcgtac cttcggtttt atgcgcgata tcgaatatct gcagtcccgc ggcctgtgcc tgggcggcag cttcgattgt gccatcgttg ttgacgatta tcgcgtactg aacgaagacg gtctgcgctt tgaagacgaa tttgttcgcc acaaaatgct ggatgcgatc ggtgacctgt ttatgtgtgg tcacaacatt atcggcgcat tcacggcgta caaatcgggt cacgcgttga acaacaaact gctgcaggcg gttctggcaa aacaggaagc ctgggagtat gtgaccttcg aagacgacgc aaaactgcca atggcgttcc gcgcaccgtc gatggttctg gcggggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t