SSGCID: Target Page

KloxA.20485.a

isochorismatase

CENTER ID:	KloxA.20485.a
ORGANISM:	Klebsiella oxytoca CAV1374
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KloxA.20485.a.B1.PW39257	Full length(KloxA.20485.a)		1	283

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|571.323.peg.1321
UniProt:	A0A318FX57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIPKLQAYA LPEASDIPAN KVNWAFEPSR AALLIHDMQE YFLNFWGENS AMMEKVVANI AALRDFCKQN GIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQQVIA ALAPDEQDTV LVKWRYSAFH RSPLEEMLKE TGRDQLIITG VYAHIGCMTT ATDAFMRDIK PFFVADALAD FSREEHLMAL KYVAGRSGRV VMTEELLPLP ASKAALRALI LPLLDESDEP LDDENLIDYG LDSVRMMALA ARWRKVHGDI DFVMLAKNPT IDAWWALLSR EVK

NT Sequence

ATGGCAATCC CTAAATTACA GGCTTACGCG CTGCCGGAAG CCAGCGATAT TCCGGCCAAC AAAGTTAACT GGGCCTTTGA GCCGTCGCGC GCCGCGCTGC TGATCCACGA TATGCAGGAG TATTTCCTCA ACTTCTGGGG CGAAAACAGC GCGATGATGG AAAAAGTGGT GGCCAATATC GCCGCCCTGC GCGACTTCTG CAAACAAAAC GGCATTCCGG TGTACTACAC CGCCCAGCCG AAAGAGCAGA GCGATGAAGA CCGCGCCCTG CTGAATGATA TGTGGGGACC GGGGTTGACC CGATCGCCGG AGCAGCAGCA GGTGATTGCC GCGCTGGCGC CGGATGAACA GGATACGGTG CTGGTGAAAT GGCGCTACAG CGCGTTTCAT CGCTCACCGC TTGAAGAGAT GCTGAAAGAG ACCGGCCGCG ACCAGCTGAT CATCACCGGC GTTTACGCCC ATATCGGCTG CATGACCACC GCCACCGACG CTTTTATGCG CGATATCAAA CCGTTCTTTG TCGCCGACGC GCTGGCAGAT TTCAGCCGCG AAGAGCATTT GATGGCGCTT AAATACGTCG CCGGCCGTTC TGGACGCGTG GTGATGACCG AAGAATTGTT GCCGCTGCCG GCCTCCAAAG CGGCGCTGCG CGCGCTAATT CTGCCGCTGC TCGACGAATC CGACGAACCG CTGGATGATG AAAACCTGAT CGACTACGGT CTGGATTCGG TGCGTATGAT GGCGCTGGCC GCCCGTTGGC GCAAAGTACA CGGCGATATC GACTTCGTGA TGCTGGCGAA AAACCCGACC ATCGACGCCT GGTGGGCGCT GCTCTCCCGC GAGGTGAAAT AA

Details for KloxA.20485.a.B1.PW39257

PURIFICATION DATe:	1/30/2024
CONCENTRATION:	15.8mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	42
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMA IPKLQAYALP EASDIPVNKV NWAFEPSRAA LLIHDMQEYF LNFWGENSAM MEKVVANIAS LRDFCKQNNI PVFYTAQPKE QSDEDRALLN DMWGPGLTRS PEQQQVIAAL APDENDTVLV KWRYSAFHRS PLEDLLKGAG RDQLIITGVY AHIGCMTTAT DAFMRDIKPF FVADALADFS REEHLMALKY VAGRSGRVVM TEELLPLPAS KAALRALVLP LLDESDEPMD DENLIDYGLD SVRMMALAAR WRKVHGDIDF VVLAKNPTID AWWTLLSREV K

Validated NT Sequence

atggctcacc accaccacca ccatatggca attcctaaat tacaggctta cgcgctgccg gaagccagcg atatcccagt gaacaaagtg aactgggcat ttgagccgtc ccgtgctgcg ctgttaatcc acgatatgca ggaatatttc ctcaatttct ggggcgaaaa cagcgcaatg atggagaaag tggtggccaa catcgcttcc ctgcgcgatt tctgcaaaca gaataatatc ccggtctttt acaccgctca gccgaaagag cagagcgacg aagatcgcgc gctgttgaac gatatgtggg ggccgggcct gacccgttcg ccggaacagc agcaggtgat cgccgctctt gcaccggacg aaaacgacac tgtgctggtg aagtggcgct acagcgcgtt tcatcgctcg ccgctggaag atctgctgaa aggagccggt cgtgatcagc tgatcatcac cggtgtttac gcccatatcg gctgtatgac gacggcgacc gacgcgttta tgcgcgatat taagccgttc ttcgttgccg atgcgctggc tgatttcagc cgtgaagagc acctgatggc gctgaaatac gtcgcgggtc gctccggtcg ggtggtgatg accgaagagc tgctgcctct accggcgtcg aaagccgcgc tgcgcgcgct ggtgctgccg ctgctggacg aatccgatga gccaatggat gacgaaaacc tgatcgacta cggcctggat tcggtgcgca tgatggcgct cgccgcccgc tggcgtaagg ttcatggcga tatcgacttt gtggtgttgg cgaaaaatcc gaccattgat gcctggtgga cgctgctttc ccgcgaggtg aaataa

Expressed Protein Sequence

MAHHHHHHMA IPKLQAYALP EASDIPANKV NWAFEPSRAA LLIHDMQEYF LNFWGENSAM MEKVVANIAA LRDFCKQNGI PVYYTAQPKE QSDEDRALLN DMWGPGLTRS PEQQQVIAAL APDEQDTVLV KWRYSAFHRS PLEEMLKETG RDQLIITGVY AHIGCMTTAT DAFMRDIKPF FVADALADFS REEHLMALKY VAGRSGRVVM TEELLPLPAS KAALRALILP LLDESDEPLD DENLIDYGLD SVRMMALAAR WRKVHGDIDF VMLAKNPTID AWWALLSREV K

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA TCCCTAAATT ACAGGCTTAC GCGCTGCCGG AAGCCAGCGA TATTCCGGCC AACAAAGTTA ACTGGGCCTT TGAGCCGTCG CGCGCCGCGC TGCTGATCCA CGATATGCAG GAGTATTTCC TCAACTTCTG GGGCGAAAAC AGCGCGATGA TGGAAAAAGT GGTGGCCAAT ATCGCCGCCC TGCGCGACTT CTGCAAACAA AACGGCATTC CGGTGTACTA CACCGCCCAG CCGAAAGAGC AGAGCGATGA AGACCGCGCC CTGCTGAATG ATATGTGGGG ACCGGGGTTG ACCCGATCGC CGGAGCAGCA GCAGGTGATT GCCGCGCTGG CGCCGGATGA ACAGGATACG GTGCTGGTGA AATGGCGCTA CAGCGCGTTT CATCGCTCAC CGCTTGAAGA GATGCTGAAA GAGACCGGCC GCGACCAGCT GATCATCACC GGCGTTTACG CCCATATCGG CTGCATGACC ACCGCCACCG ACGCTTTTAT GCGCGATATC AAACCGTTCT TTGTCGCCGA CGCGCTGGCA GATTTCAGCC GCGAAGAGCA TTTGATGGCG CTTAAATACG TCGCCGGCCG TTCTGGACGC GTGGTGATGA CCGAAGAATT GTTGCCGCTG CCGGCCTCCA AAGCGGCGCT GCGCGCGCTA ATTCTGCCGC TGCTCGACGA ATCCGACGAA CCGCTGGATG ATGAAAACCT GATCGACTAC GGTCTGGATT CGGTGCGTAT GATGGCGCTG GCCGCCCGTT GGCGCAAAGT ACACGGCGAT ATCGACTTCG TGATGCTGGC GAAAAACCCG ACCATCGACG CCTGGTGGGC GCTGCTCTCC CGCGAGGTGA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT