SSGCID: Target Page

KloxA.17981.a

Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase) (ProRS)

CENTER ID:	KloxA.17981.a
ORGANISM:	Klebsiella oxytoca CAV1374
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KloxA.17981.a.A1.PW39321	full length		1	572
KloxA.17981.a.A1.PW39264	full length		1	572

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A2X4YQ58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGVRVL KKVENIVREE MNNAGAIEVL MPVVQPSELW QESGRWEQYG PELLRIADRG ERPFVLGPTH EEVITDLIRN ELSSYKQLPL NFYQIQTKFR DEVRPRFGVM RSREFLMKDA YSFHTSQESL QETYDAMYAA YSKIFSRMGL DFRAVQADTG SIGGSASHEF QVLAQSGEDD VIFSDSSDYA ANIEFAEAVA PKEPRAAATQ EMTLVDTPDA KTIAELVEQF NLPIEKTVKT LLVKAVEDNE YPLIALLVRG DHELNEVKAE KLPQVASPLT FATEEEIRAL VKAGPGSLGP VNMPVPVIID RTVAAMSDFA AGANIDGKHY FGINWDRDVA TPEVADIRNV VAGDPSPDGK GTLLIKRGIE VGHIFQLGTK YSEAMKASVQ GEDGRNQILT MGCYGIGVTR VVAAAIEQNF DERGILWPEA IAPFQVAILP MNMHKSYRVQ ELAEKLYAEL TALGIEVLMD DRKERPGVMF ADMELIGIPH TIVLGDRNLD NDDIEYKYRR SGEKQLIKTG DIVNYLVQQI KG

NT Sequence

atgcgtacta gccaatacct gctctccact ctgaaggaga cacctgccga cgccgaggtt atcagccatc agctgatgct gcgcgccggg atgatccgca agctggcctc tgggttatat acctggctgc cgaccggcgt gcgcgtcctg aaaaaagtcg aaaacatcgt gcgtgaagag atgaacaacg ctggtgcaat cgaggtgtta atgccggtag ttcagccctc tgagctgtgg caagagagtg gtcgttggga acagtacggc ccggaactgc tgcgtattgc tgaccgtggc gaacgtccgt tcgtactcgg cccgactcat gaagaagtca tcaccgacct gattcgtaat gagctgagct cttacaaaca gctgccgctg aacttctatc agatccagac caaattccgt gacgaagttc gcccgcgttt cggcgtaatg cgttcccgtg aattcctgat gaaagatgct tactctttcc atacctctca ggaatcgctg caggagacct acgacgcgat gtacgccgcg tacagcaaaa tcttcagccg tatggggctg gatttccgcg cggtacaggc tgacaccggt tctatcggcg gcagcgcatc tcacgaattc caggtactgg cgcagagcgg tgaagatgac gtcattttct ctgactcttc tgactacgcg gccaatattg agttcgccga agccgttgcg ccgaaagagc cgcgcgcggc cgccacgcag gaaatgaccc tggtcgatac gcctgacgct aaaaccatcg ccgagctggt cgagcagttc aatctgccga ttgaaaaaac cgtgaaaacc ctgctggtaa aagcagtaga agataatgag tatccgctga tagccctgct ggttcgcggc gatcatgaac tgaacgaagt caaagcggaa aaactgccgc aggtcgccag cccgctgacc ttcgcaaccg aagaagagat tcgcgcgctg gtgaaagcgg gtcccggttc tcttggcccg gttaatatgc ctgttccggt gattattgac cgtaccgttg ccgccatgag cgatttcgcc gcgggcgcga acattgacgg taaacactac ttcggcatca actgggatcg cgacgtcgcc actcctgaag tggcagatat ccgtaacgta gtggccggtg acccgagccc ggacggtaaa ggcacgctgt tgattaaacg cggtatcgaa gtgggccaca tcttccagct gggtaccaag tactctgaag cgatgaaagc ctccgtacag ggcgaagatg gccgtaatca gatcctgact atgggctgct acggtatcgg ggtgactcgc gtggtggcgg cagccattga gcagaacttt gacgagcgcg ggatcttgtg gccggaagct atcgccccgt tccaggttgc gattctgccg atgaacatgc acaaatccta tcgcgtgcag gagctggctg agaagctgta tgctgagcta accgcgctgg gcatcgaagt gctgatggat gaccgtaaag agcgtccggg cgtgatgttc gccgatatgg agcttatcgg aattccgcat actatcgtgc tgggcgaccg taacctcgac aacgacgata ttgaatataa atatcgccgc agcggcgaga aacagctgat taaaaccggc gatatcgtga attacctggt acaacaaatt aaaggttaa

Details for KloxA.17981.a.A1.PW39321

PURIFICATION DATe:	10/3/2024
CONCENTRATION:	12.6mg/ml
OBSERVED MW:	66kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 30 mM Imidazole, 0.025% Azide, 1 mM TCEP *3C cleavage was performed on original protein stock to remove His-tag; only reverse IMAC was performed, no SEC since it was performed in the original purification.
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRTSQYLLS TLKETPADAE VISHQLMLRA GMIRKLASGL YTWLPTGVRV LKKVENIVRE EMNNAGAIEV LMPVVQPSEL WQESGRWEQY GPELLRIADR GERPFVLGPT HEEVITDLIR NELSSYKQLP LNFYQIQTKF RDEVRPRFGV MRSREFLMKD AYSFHTSQES LQETYDAMYA AYSKIFSRMG LDFRAVQADT GSIGGSASHE FQVLAQSGED DVIFSDSSDY AANIEFAEAV APKEPRAAAT QEMTLVDTPD AKTIAELVEQ FNLPIEKTVK TLLVKAVEDN EYPLIALLVR GDHELNEVKA EKLPQVASPL TFATEEEIRA LVKAGPGSLG PVNMPVPVII DRTVAAMSDF AAGANIDGKH YFGINWDRDV ATPEVADIRN VVAGDPSPDG KGTLLIKRGI EVGHIFQLGT KYSEAMKASV QGEDGRNQIL TMGCYGIGVT RVVAAAIEQN FDERGILWPE AIAPFQVAIL PMNMHKSYRV QELAEKLYAE LTALGIEVLM DDRKERPGVM FADMELIGIP HTIVLGDRNL DNDDIEYKYR RSGEKQLIKT GDIVNYLVQQ IKG

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTACTAGC CAATACCTGC TCTCCACTCT GAAGGAGACA CCTGCCGACG CCGAGGTTAT CAGCCATCAG CTGATGCTGC GCGCCGGGAT GATCCGCAAG CTGGCCTCTG GGTTATATAC CTGGCTGCCG ACCGGCGTGC GCGTCCTGAA AAAAGTCGAA AACATCGTGC GTGAAGAGAT GAACAACGCT GGTGCAATCG AGGTGTTAAT GCCGGTAGTT CAGCCCTCTG AGCTGTGGCA AGAGAGTGGT CGTTGGGAAC AGTACGGCCC GGAACTGCTG CGTATTGCTG ACCGTGGCGA ACGTCCGTTC GTACTCGGCC CGACTCATGA AGAAGTCATC ACCGACCTGA TTCGTAATGA GCTGAGCTCT TACAAACAGC TGCCGCTGAA CTTCTATCAG ATCCAGACCA AATTCCGTGA CGAAGTTCGC CCGCGTTTCG GCGTAATGCG TTCCCGTGAA TTCCTGATGA AAGATGCTTA CTCTTTCCAT ACCTCTCAGG AATCGCTGCA GGAGACCTAC GACGCGATGT ACGCCGCGTA CAGCAAAATC TTCAGCCGTA TGGGGCTGGA TTTCCGCGCG GTACAGGCTG ACACCGGTTC TATCGGCGGC AGCGCATCTC ACGAATTCCA GGTACTGGCG CAGAGCGGTG AAGATGACGT CATTTTCTCT GACTCTTCTG ACTACGCGGC CAATATTGAG TTCGCCGAAG CCGTTGCGCC GAAAGAGCCG CGCGCGGCCG CCACGCAGGA AATGACCCTG GTCGATACGC CTGACGCTAA AACCATCGCC GAGCTGGTCG AGCAGTTCAA TCTGCCGATT GAAAAAACCG TGAAAACCCT GCTGGTAAAA GCAGTAGAAG ATAATGAGTA TCCGCTGATA GCCCTGCTGG TTCGCGGCGA TCATGAACTG AACGAAGTCA AAGCGGAAAA ACTGCCGCAG GTCGCCAGCC CGCTGACCTT CGCAACCGAA GAAGAGATTC GCGCGCTGGT GAAAGCGGGT CCCGGTTCTC TTGGCCCGGT TAATATGCCT GTTCCGGTGA TTATTGACCG TACCGTTGCC GCCATGAGCG ATTTCGCCGC GGGCGCGAAC ATTGACGGTA AACACTACTT CGGCATCAAC TGGGATCGCG ACGTCGCCAC TCCTGAAGTG GCAGATATCC GTAACGTAGT GGCCGGTGAC CCGAGCCCGG ACGGTAAAGG CACGCTGTTG ATTAAACGCG GTATCGAAGT GGGCCACATC TTCCAGCTGG GTACCAAGTA CTCTGAAGCG ATGAAAGCCT CCGTACAGGG CGAAGATGGC CGTAATCAGA TCCTGACTAT GGGCTGCTAC GGTATCGGGG TGACTCGCGT GGTGGCGGCA GCCATTGAGC AGAACTTTGA CGAGCGCGGG ATCTTGTGGC CGGAAGCTAT CGCCCCGTTC CAGGTTGCGA TTCTGCCGAT GAACATGCAC AAATCCTATC GCGTGCAGGA GCTGGCTGAG AAGCTGTATG CTGAGCTAAC CGCGCTGGGC ATCGAAGTGC TGATGGATGA CCGTAAAGAG CGTCCGGGCG TGATGTTCGC CGATATGGAG CTTATCGGAA TTCCGCATAC TATCGTGCTG GGCGACCGTA ACCTCGACAA CGACGATATT GAATATAAAT ATCGCCGCAG CGGCGAGAAA CAGCTGATTA AAACCGGCGA TATCGTGAAT TACCTGGTAC AACAAATTAA AGGTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA

Details for KloxA.17981.a.A1.PW39264

PURIFICATION DATe:	2/21/2024
CONCENTRATION:	16.8mg/ml
OBSERVED MW:	66kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	31
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)