KloxA.01187.a

Glutamyl-tRNA synthetase (EC 6.1.1.17) (GluRS) (GltX)

CENTER ID: KloxA.01187.a
ORGANISM: Klebsiella oxytoca CAV1374
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KloxA.01187.a.B1.PW39252 Full length(KloxA.01187.a) 1 472

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1028307.3.peg.54
UniProt: A0A0H3FQ37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIKTRFAPS PTGYLHVGGA RTALYSWLFA RNHGGEFVLR IEDTDLERST PEAIEAIMDG MNWLNLEWDE GPYFQTKRFD RYNAVIDEML EAGTAYKCYC SKERLDALRE EQMANGEKPR YDGRCRHDHQ HHAADEPCVV RFANPQEGSV IFDDQIRGPI EFSNQELDDL IIRRTDGSPT YNFCVVVDDW DMAITHVIRG EDHINNTPRQ INILKALNAP VPVYAHVSMI NGDDGKKLSK RHGAVSVMQY RDDGYLPEAL LNYLVRLGWS HGDQEIFTRE EMIEFFSLGA VSKSASAFNT DKLLWLNHHY INTLPAEYVA THLQWHIEQE NIDTRNGPQL SELVKLLGER CKTLKEIAQS CRYFYEEFAE FDADAAKKHL RPVARQPLEV VRDKLAAISD WTAENVHHAI QATADELEVG MGKVGMPLRV AVTGAGQSPA LDVTVHAIGK SRSIERINKA LAFIAERESQ QS
NT Sequence
ATGAAAATCA AAACTCGCTT CGCGCCAAGC CCAACAGGCT ATCTGCACGT TGGCGGTGCG CGTACTGCTC TCTATTCCTG GCTTTTTGCA CGTAACCACG GCGGTGAGTT TGTGCTGCGT ATTGAAGACA CCGATCTCGA ACGCTCCACC CCGGAAGCAA TTGAAGCCAT CATGGATGGG ATGAACTGGC TGAATCTGGA GTGGGATGAA GGTCCGTACT TCCAGACTAA ACGTTTCGAT CGCTATAACG CGGTTATTGA TGAAATGCTG GAAGCAGGTA CCGCTTATAA ATGCTACTGC TCGAAAGAAC GTCTGGACGC GCTGCGCGAA GAACAGATGG CGAACGGTGA AAAGCCGCGC TACGACGGCC GCTGCCGTCA TGATCATCAG CATCACGCCG CCGATGAGCC TTGCGTCGTG CGTTTTGCCA ACCCGCAGGA AGGTTCGGTT ATCTTTGACG ACCAGATCCG CGGGCCGATC GAATTCAGCA ACCAGGAGCT GGACGATCTG ATCATTCGCC GCACCGACGG CTCCCCGACC TATAACTTCT GCGTCGTAGT TGATGACTGG GATATGGCGA TCACCCACGT GATCCGCGGT GAAGACCACA TCAACAATAC CCCGCGCCAG ATCAATATCC TGAAAGCGCT GAATGCGCCG GTGCCGGTTT ATGCGCACGT ATCGATGATC AACGGCGATG ACGGTAAAAA ACTTTCCAAG CGCCACGGTG CGGTGAGTGT GATGCAGTAC CGTGACGACG GCTACCTGCC GGAAGCGTTG CTGAACTATC TGGTGCGTCT GGGCTGGTCC CATGGCGATC AGGAGATCTT CACCCGCGAA GAGATGATCG AGTTCTTCTC GCTGGGCGCG GTCAGCAAGT CCGCCAGTGC GTTCAACACC GATAAACTGC TGTGGTTAAA CCATCACTAC ATCAACACGC TGCCGGCAGA ATATGTCGCG ACGCATCTGC AGTGGCACAT TGAGCAGGAA AATATCGATA CCCGTAACGG CCCGCAGTTG TCCGAACTGG TTAAACTGCT GGGCGAGCGT TGCAAAACGC TGAAAGAGAT TGCCCAGAGC TGCCGCTACT TCTATGAAGA GTTCGCCGAG TTCGACGCCG ATGCGGCGAA GAAACACCTA CGTCCGGTGG CGCGTCAGCC GCTGGAAGTG GTTCGCGATA AGCTGGCGGC CATTAGCGAC TGGACAGCGG AAAATGTCCA TCATGCGATT CAGGCGACCG CTGATGAGCT GGAAGTGGGC ATGGGGAAAG TGGGCATGCC GCTGCGTGTG GCCGTGACCG GCGCGGGTCA ATCTCCGGCG CTGGACGTCA CCGTTCATGC GATCGGTAAA TCCCGTAGCA TTGAACGTAT CAACAAAGCG CTGGCGTTTA TCGCCGAACG TGAAAGCCAG CAGTCCTGA
Details for KloxA.01187.a.B1.PW39252
PURIFICATION DATe: 1/8/2024
CONCENTRATION: 15.6mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 34
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK IKTRFAPSPT GYLHVGGART ALYSWLFARN HGGEFVLRIE DTDLERSTPE AIEAIMDGMN WLNLEWDEGP YFQTKRFDRY NAVIDEMLEA GTAYKCYCSK ERLDALREEQ MANGEKPRYD GRCRHDHQHH AADEPCVVRF ANPQEGSVIF DDQIRGPIEF SNQELDDLII RRTDGSPTYN FCVVVDDWDM AITHVIRGED HINNTPRQIN ILKALNAPVP VYAHVSMING DDGKKLSKRH GAVSVMQYRD DGYLPEALLN YLVRLGWSHG DQEIFTREEM IEFFSLGAVS KSASAFNTDK LLWLNHHYIN TLPAEYVATH LQWHIEQENI DTRNGPQLSE LVKLLGERCK TLKEIAQSCR YFYEEFAEFD ADAAKKHLRP VARQPLEVVR DKLAAISDWT AENVHHAIQA TADELEVGMG KVGMPLRVAV TGAGQSPALD VTVHAIGKSR SIERINKALA FIAERESQQS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA TCAAAACTCG CTTCGCGCCA AGCCCAACAG GCTATCTGCA CGTTGGCGGT GCGCGTACTG CTCTCTATTC CTGGCTTTTT GCACGTAACC ACGGCGGTGA GTTTGTGCTG CGTATTGAAG ACACCGATCT CGAACGCTCC ACCCCGGAAG CAATTGAAGC CATCATGGAT GGGATGAACT GGCTGAATCT GGAGTGGGAT GAAGGTCCGT ACTTCCAGAC TAAACGTTTC GATCGCTATA ACGCGGTTAT TGATGAAATG CTGGAAGCAG GTACCGCTTA TAAATGCTAC TGCTCGAAAG AACGTCTGGA CGCGCTGCGC GAAGAACAGA TGGCGAACGG TGAAAAGCCG CGCTACGACG GCCGCTGCCG TCATGATCAT CAGCATCACG CCGCCGATGA GCCTTGCGTC GTGCGTTTTG CCAACCCGCA GGAAGGTTCG GTTATCTTTG ACGACCAGAT CCGCGGGCCG ATCGAATTCA GCAACCAGGA GCTGGACGAT CTGATCATTC GCCGCACCGA CGGCTCCCCG ACCTATAACT TCTGCGTCGT AGTTGATGAC TGGGATATGG CGATCACCCA CGTGATCCGC GGTGAAGACC ACATCAACAA TACCCCGCGC CAGATCAATA TCCTGAAAGC GCTGAATGCG CCGGTGCCGG TTTATGCGCA CGTATCGATG ATCAACGGCG ATGACGGTAA AAAACTTTCC AAGCGCCACG GTGCGGTGAG TGTGATGCAG TACCGTGACG ACGGCTACCT GCCGGAAGCG TTGCTGAACT ATCTGGTGCG TCTGGGCTGG TCCCATGGCG ATCAGGAGAT CTTCACCCGC GAAGAGATGA TCGAGTTCTT CTCGCTGGGC GCGGTCAGCA AGTCCGCCAG TGCGTTCAAC ACCGATAAAC TGCTGTGGTT AAACCATCAC TACATCAACA CGCTGCCGGC AGAATATGTC GCGACGCATC TGCAGTGGCA CATTGAGCAG GAAAATATCG ATACCCGTAA CGGCCCGCAG TTGTCCGAAC TGGTTAAACT GCTGGGCGAG CGTTGCAAAA CGCTGAAAGA GATTGCCCAG AGCTGCCGCT ACTTCTATGA AGAGTTCGCC GAGTTCGACG CCGATGCGGC GAAGAAACAC CTACGTCCGG TGGCGCGTCA GCCGCTGGAA GTGGTTCGCG ATAAGCTGGC GGCCATTAGC GACTGGACAG CGGAAAATGT CCATCATGCG ATTCAGGCGA CCGCTGATGA GCTGGAAGTG GGCATGGGGA AAGTGGGCAT GCCGCTGCGT GTGGCCGTGA CCGGCGCGGG TCAATCTCCG GCGCTGGACG TCACCGTTCA TGCGATCGGT AAATCCCGTA GCATTGAACG TATCAACAAA GCGCTGGCGT TTATCGCCGA ACGTGAAAGC CAGCAGTCCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT