KlaeA.18849.a

Valine--tRNA ligase (EC 6.1.1.9) (Valyl-tRNA synthetase) (ValRS)

CENTER ID: KlaeA.18849.a
ORGANISM: Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlaeA.18849.a.UX1.PW39269 full length 1 951

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1028307.3.peg.1940
UniProt: A0A0H3FN79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKTYNPQDI EQPLYEHWEK QGYFKPNGDE SQESFCIMIP PPNVTGSLHM GHAFQQTIMD TMIRYQRMQG KNTLWQAGTD HAGIATQMVV ERKIAAEEGK TRHDYGRDAF IDKIWQWKAE SGGTITRQMR RLGNSVDWER ERFTMDEGLS NAVKEVFVRL YKEDLIYRGK RLVNWDPKLR TAISDLEVEN RESKGSMWHI RYPLADGAKT ADGKDYLVVA TTRPETLLGD TGVAVNPEDP RYKDLIGKFV VLPLVNRRIP IVGDEHADME KGTGCVKITP AHDFNDYEVG RRHQLPMINI LTFDGDIRES AEVYDTKGNE SDVYSNEIPA EFQKMERFAA RKAMVAAVDA LGLLEEIKPH DLTVPYGDRG GVVIEPMLTD QWYVRADVLA KPAVEAVENG DIQFVPKQYE NMYFSWMRDI QDWCISRQLW WGHRIPAWYD DQGNVYVGRT EDEVRQENNL GADVALRQDE DVLDTWFSSA LWTFSTLGWP ENTDALRQFH PTSVMVSGFD IIFFWIARMI MMTMHFIKDE NGKPQVPFKT VYMTGLIRDD EGQKMSKSKG NVIDPLDMVD GITLPELLEK RTGNMMQPQL AEKIRKRTEK QFPNGIEPHG TDALRFTLAA LASTGRDINW DMKRLEGYRN FCNKLWNASR FVLMNTEDQD CGFNGGEMVL SLADRWILAE FNQTVKAYRE ALDNFRFDIA AGILYEFTWN QFCDWYLELT KPVMNGGSEA ELRGTRHTLV TVLEGLLRLA HPIIPFITET IWQRVKVICG ITADTIMLQP FPQYEASQVD EAALADTEWL KQAIVAVRNI RAEMNIAPGK PLELLLRGCS KDAERRVNDN RSFLLNLARL ESITVLPADD KGPVSVTKII DGAELLIPMA GLIDKEAELA RLAKEVAKVE AEIGRIESKL SNEGFVARAP EAVIAKEREK LEGYAEAKAK LIEQQAVIAA L
NT Sequence
atggaaaaga catacaaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctatt tcaaaccgaa tggtgatgaa agccaggaga gcttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcatgcct tccagcaaac catcatggat accatgattc gctaccagcg catgcagggt aaaaacaccc tgtggcaggc cggtaccgac cacgcgggta tcgctaccca aatggtggtt gagcgtaaga ttgccgctga agaaggtaaa acccgccacg actacggccg cgatgccttc atcgataaaa tctggcagtg gaaagcggaa tccggcggta ccattacccg ccagatgcgc cgcctcggca actccgtcga ctgggagcgc gagcgcttca cgatggatga aggcctgtcc aacgcggtaa aagaagtctt cgttcgcctg tacaaagaag acctgattta tcgcggcaag cgcctggtca actgggaccc gaaactgcgc accgccatct ctgacctgga agtagagaac cgcgaatcga aaggttccat gtggcacatc cgctatccgc tggccgatgg cgcgaaaacc gctgacggta aagattacct ggtggtcgcc accacccgtc cggaaaccct gttgggcgat accggcgtgg ccgttaaccc ggaagatccg cgttacaaag acctgatcgg taaattcgtg gtgctgccgc tggttaaccg ccgcattcca attgttggcg acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa aatcaccccg gcgcacgact ttaacgacta cgaagttggc cgccgtcatc agctgccgat gattaatatt ttgacctttg atggcgacat ccgcgaaagc gcggaagtct acgataccaa aggcaacgaa tccgacgtct attccaacga gatcccggct gaattccaga agatggaacg ttttgccgcg cgtaaagcga tggttgccgc cgtcgacgcg ttgggcctgc tggaagagat caaaccgcac gacctgaccg tgccttacgg cgaccgcggc ggcgtggtta tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga cgtgctggcc aagccggcag tcgaagccgt tgagaacggc gacattcagt tcgtgccgaa gcagtacgaa aacatgtact tctcctggat gcgcgatatt caggactggt gtatttcccg tcagctgtgg tggggtcacc gcatcccggc atggtacgac gatcagggca acgtctacgt tggccgcacc gaagacgaag tacgccagga aaataatctc ggcgccgacg tcgcgctgcg tcaggacgaa gacgtccttg atacctggtt ctcttccgcg ctgtggacct tctccaccct cggctggccg gaaaacaccg acgcgctgcg tcagttccac ccgaccagcg tgatggtgtc cggcttcgac attatcttct tctggattgc ccgcatgatc atgatgacca tgcacttcat taaagatgaa aacggcaagc cgcaggttcc gtttaagacc gtctacatga ccggccttat ccgcgacgac gaaggccaga agatgtctaa atccaagggt aacgtcattg acccgctgga tatggttgac ggcatcaccc tgccggagct gctggagaaa cgtaccggta acatgatgca gccgcagctg gcggaaaaaa tccgtaagcg caccgagaaa cagttcccga acggcatcga gccgcacggc accgacgccc tgcgtttcac cctggcggcg ctggcctcta ccggccgcga catcaactgg gatatgaagc gtctggaagg ttaccgtaac ttctgcaaca agctgtggaa cgccagccgc ttcgtactga tgaataccga agatcaggat tgtggtttta atggcggcga gatggtgctg tccctggccg accgctggat cctggctgaa ttcaaccaga ccgtgaaagc gtaccgcgaa gcgctggata acttccgctt cgacatcgcc gcgggcatcc tgtatgaatt tacctggaac cagttctgcg actggtacct cgaactgacc aagccggtca tgaacggtgg cagcgaagcg gagctgcgcg ggactcgcca tacgctggtg accgtgctgg aaggtctgct gcgcctggcg catccgatca tcccgttcat caccgagacc atctggcagc gcgtgaaagt aatctgcggt atcaccgccg ataccatcat gctgcaaccg ttcccgcagt acgaggcttc tcaggttgat gaagcggcgc tagccgacac cgaatggctg aagcaggcga tcgtcgccgt acgtaacatc cgtgcggaga tgaacatcgc tccgggtaaa ccgctggaac tgctgctgcg cggctgtagc aaagatgccg agcgtcgtgt taacgacaac cgcagcttcc tgctgaacct ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgat aaaggtccgg tttccgtgac caagattatc gacggcgccg agctgctgat cccgatggcg ggtcttatcg acaaagaagc cgagctggcg cgtctggcaa aagaagtggc gaaagtcgaa gcggaaatcg gccgtatcga aagcaagctg tccaacgaag gctttgtggc ccgcgcgccg gaagcggtta tcgccaaaga gcgtgagaag ctggaaggct acgcggaagc gaaagcgaaa ctgattgaac agcaggcagt gatcgccgcg ctgtaa
Details for KlaeA.18849.a.UX1.PW39269
PURIFICATION DATe: 3/12/2024
CONCENTRATION: 11.8mg/ml
OBSERVED MW: 111kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKTYNPQD IEQPLYEHWE KQGYFKPNGD ESQESFCIMI PPPNVTGSLH MGHAFQQTIM DTMIRYQRMQ GKNTLWQAGT DHAGIATQMV VERKIAAEEG KTRHDYGRDA FIDKIWQWKA ESGGTITRQM RRLGNSVDWE RERFTMDEGL SNAVKEVFVR LYKEDLIYRG KRLVNWDPKL RTAISDLEVE NRESKGSMWH IRYPLADGAK TADGKDYLVV ATTRPETLLG DTGVAVNPED PRYKDLIGKF VVLPLVNRRI PIVGDEHADM EKGTGCVKIT PAHDFNDYEV GRRHQLPMIN ILTFDGDIRE SAEVYDTKGN ESDVYSNEIP AEFQKMERFA ARKAMVAAVD ALGLLEEIKP HDLTVPYGDR GGVVIEPMLT DQWYVRADVL AKPAVEAVEN GDIQFVPKQY ENMYFSWMRD IQDWCISRQL WWGHRIPAWY DDQGNVYVGR TEDEVRQENN LGADVALRQD EDVLDTWFSS ALWTFSTLGW PENTDALRQF HPTSVMVSGF DIIFFWIARM IMMTMHFIKD ENGKPQVPFK TVYMTGLIRD DEGQKMSKSK GNVIDPLDMV DGITLPELLE KRTGNMMQPQ LAEKIRKRTE KQFPNGIEPH GTDALRFTLA ALASTGRDIN WDMKRLEGYR NFCNKLWNAS RFVLMNTEDQ DCGFNGGEMV LSLADRWILA EFNQTVKAYR EALDNFRFDI AAGILYEFTW NQFCDWYLEL TKPVMNGGSE AELRGTRHTL VTVLEGLLRL AHPIIPFITE TIWQRVKVIC GITADTIMLQ PFPQYEASQV DEAALADTEW LKQAIVAVRN IRAEMNIAPG KPLELLLRGC SKDAERRVND NRSFLLNLAR LESITVLPAD DKGPVSVTKI IDGAELLIPM AGLIDKEAEL ARLAKEVAKV EAEIGRIESK LSNEGFVARA PEAVIAKERE KLEGYAEAKA KLIEQQAVIA AL
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA ATTAATTCTT AGAAAAACTC ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGTTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TAGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCTCTAG AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACCATGG CTCATCACCA TCACCATCAT ATGGGTACCC TGGAAGCTCA GACCCAGGGT CCTGGTTCGA TGGAGAAAAC CTATAATCCG CAGGACATTG AGCAACCTCT GTATGAACAT TGGGAGAAAC AAGGTTACTT TAAGCCTAAC GGCGACGAGT CCCAAGAATC TTTCTGTATT ATGATTCCAC CCCCAAATGT AACTGGTTCC CTTCACATGG GACACGCATT TCAACAGACG ATTATGGACA CAATGATCAG ATATCAACGT ATGCAAGGAA AGAATACGTT ATGGCAAGCT GGGACTGATC ATGCCGGCAT TGCCACGCAG ATGGTCGTGG AACGCAAAAT CGCTGCCGAA GAGGGAAAGA CTAGACATGA TTATGGTCGT GACGCTTTTA TTGACAAGAT ATGGCAATGG AAGGCTGAGT CAGGTGGAAC GATCACTCGA CAAATGCGAC GTCTTGGTAA TAGTGTTGAT TGGGAACGTG AACGTTTTAC CATGGACGAG GGACTTTCTA ATGCCGTTAA GGAAGTTTTC GTGCGTCTTT ATAAGGAAGA TCTCATCTAC CGGGGTAAAC GTTTGGTAAA TTGGGATCCT AAGCTTAGAA CAGCGATTAG CGATTTAGAG GTTGAAAATC GAGAGTCAAA GGGCTCAATG TGGCATATTC GTTACCCATT GGCTGACGGT GCCAAGACTG CCGATGGCAA GGACTATTTG GTAGTGGCAA CTACACGCCC CGAGACACTT CTGGGCGACA CAGGTGTTGC AGTAAATCCA GAGGACCCCC GGTATAAGGA TTTAATAGGA AAGTTTGTCG TTTTGCCTTT GGTCAATCGT CGGATACCGA TCGTAGGTGA TGAGCATGCT GATATGGAGA AGGGTACGGG ATGTGTAAAG ATAACACCAG CCCATGATTT CAATGATTAT GAGGTCGGAC GGCGCCACCA ATTACCCATG ATAAACATAT TAACGTTCGA CGGAGATATT CGTGAGTCTG CAGAGGTATA TGACACTAAG GGAAATGAGA GCGATGTTTA CTCGAATGAA ATTCCAGCGG AGTTTCAAAA GATGGAGCGC TTCGCAGCCC GGAAGGCTAT GGTAGCTGCG GTTGATGCCC TGGGATTACT TGAGGAAATT AAGCCCCATG ATTTGACTGT CCCGTATGGA GATAGAGGTG GAGTTGTGAT TGAGCCTATG CTTACAGATC AATGGTATGT TAGAGCTGAT GTTTTGGCAA AACCAGCGGT CGAGGCTGTA GAAAATGGTG ATATACAATT TGTTCCTAAA CAATACGAGA ACATGTACTT TTCGTGGATG AGAGACATAC AAGATTGGTG CATCTCAAGA CAACTTTGGT GGGGCCATCG GATACCTGCG TGGTATGATG ACCAAGGTAA TGTTTATGTA GGTCGGACTG AGGATGAGGT GAGACAAGAG AACAACTTGG GAGCGGATGT GGCTTTAAGA CAAGATGAGG ATGTTCTCGA CACATGGTTT AGTAGCGCCT TGTGGACTTT TAGTACTCTG GGATGGCCAG AGAATACGGA TGCTCTTCGC CAATTTCATC CAACTTCCGT TATGGTTTCG GGGTTTGACA TAATTTTCTT TTGGATAGCG CGTATGATCA TGATGACGAT GCATTTTATA AAGGACGAGA ATGGAAAACC ACAAGTGCCT TTCAAAACAG TGTATATGAC TGGACTGATA CGTGATGATG AGGGTCAAAA GATGAGCAAG TCGAAAGGGA ATGTTATCGA TCCATTGGAC ATGGTAGATG GTATAACGCT TCCCGAACTT TTAGAAAAGC GGACTGGCAA TATGATGCAA CCACAATTAG CAGAGAAGAT ACGCAAACGT ACAGAAAAGC AATTTCCAAA TGGAATAGAA CCTCATGGTA CTGATGCTCT TCGGTTTACG TTAGCTGCCT TAGCTAGCAC AGGTCGTGAT ATTAATTGGG ACATGAAACG CTTAGAGGGC TATCGAAATT TCTGTAATAA ACTTTGGAAT GCGTCCAGAT TTGTTCTCAT GAACACAGAG GACCAAGACT GCGGCTTCAA CGGTGGTGAA ATGGTATTAT CTCTTGCAGA TCGATGGATA TTAGCAGAGT TTAATCAAAC AGTAAAGGCC TATCGAGAGG CTTTGGACAA TTTTCGTTTT GATATTGCGG CTGGGATTCT TTACGAGTTC ACTTGGAATC AATTTTGTGA TTGGTATCTT GAGCTCACGA AACCCGTAAT GAATGGTGGT TCAGAGGCCG AACTTAGAGG CACACGGCAC ACCTTAGTAA CGGTTCTCGA GGGGCTCTTA AGATTGGCAC ACCCTATAAT TCCATTTATT ACTGAAACGA TTTGGCAACG TGTCAAGGTT ATTTGTGGAA TTACGGCTGA CACGATTATG CTTCAGCCTT TTCCTCAATA TGAAGCGAGT CAAGTCGACG AGGCTGCCTT GGCTGATACG GAGTGGCTCA AACAAGCCAT AGTGGCAGTG CGGAATATTC GGGCCGAAAT GAATATTGCC CCTGGAAAGC CACTTGAGTT GTTACTTCGG GGTTGCTCGA AGGACGCTGA AAGAAGAGTC AATGATAATC GAAGTTTTCT TCTTAATCTT GCACGATTAG AGTCCATAAC AGTCCTGCCA GCAGACGACA AGGGACCCGT CAGCGTTACT AAAATAATTG ATGGTGCAGA ATTATTAATT CCAATGGCAG GCTTAATTGA TAAAGAGGCT GAATTAGCCA GATTAGCCAA AGAGGTAGCT AAGGTTGAGG CCGAGATTGG AAGAATAGAG TCTAAACTCA GCAATGAGGG GTTCGTAGCA CGGGCACCAG AGGCCGTGAT AGCGAAGGAA CGCGAAAAGC TTGAGGGGTA TGCAGAGGCA AAGGCAAAGC TTATAGAGCA ACAAGCTGTC ATTGCGGCCC TCTGACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT