KlaeA.18181.a

Histidinol dehydrogenase (HisD)

CENTER ID: KlaeA.18181.a
ORGANISM: Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlaeA.18181.a.B1.PW39182 Full length(KlaeA.18181.a) 1 434

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFNTIIDWN GCSAEQQQQL LTRPAISASE SISKTVSEIL NNVKDNGDAA LREYSAKFDK TDVKALKVSD EEIAAAGARL SDELKQAMAV AVKNIETFHN AQRLQAVDVE TLPGVRCQQV TRPIASVGLY IPGGSAPLFS TVLMLATPAR IAGCQQVVLC SPPPIADEIL YAAQLCGVRN IFNVGGAQAI AALAFGTESV AKVDKIFGPG NAFVTEAKRQ VSQRLDGAAI DMPAGPSEVL VIADSGATPD FVASDLLSQA EHGPDSQVIL LTPDSEMATR VAQAVERQLA ALSRAETARG ALSASRIIVA RDIAQCVEIS NQYGPEHLII QTRNARELVD GITSAGSVFL GDWSPESAGD YASGTNHVLP TYGYTATCSS LGLADFQKRM TVQELSRDGF AALASTIEIL AAAERLDAHK NAVTLRVAAL KEQA
NT Sequence
ATGAGCTTCA ACACAATTAT CGACTGGAAC GGCTGTAGCG CTGAACAACA GCAACAACTC TTAACGCGCC CGGCCATTTC CGCCTCCGAG AGCATCAGCA AGACGGTGTC GGAGATCCTC AATAACGTCA AAGACAATGG CGATGCGGCT CTGCGTGAAT ATAGCGCGAA GTTCGATAAA ACCGACGTCA AGGCGCTGAA GGTCAGCGAT GAAGAGATTG CCGCCGCCGG CGCGCGCCTG AGCGACGAGC TCAAGCAGGC GATGGCCGTG GCGGTCAAAA ATATCGAAAC CTTCCACAAC GCGCAGCGGC TGCAGGCCGT CGATGTGGAA ACCCTGCCGG GCGTACGCTG CCAGCAGGTG ACCCGCCCTA TCGCCTCCGT CGGCTTATAT ATTCCCGGCG GCTCGGCGCC GCTGTTCTCC ACGGTGCTGA TGCTCGCCAC GCCGGCGCGC ATCGCGGGCT GCCAGCAGGT GGTGCTATGC TCGCCGCCGC CGATTGCCGA TGAAATCCTC TATGCCGCCC AGCTGTGCGG CGTGCGCAAT ATCTTTAACG TCGGCGGCGC GCAGGCGATT GCCGCCCTGG CGTTCGGCAC CGAATCGGTG GCGAAGGTCG ATAAAATCTT TGGCCCGGGC AACGCCTTCG TGACCGAAGC CAAGCGCCAG GTCAGCCAGC GTCTTGACGG CGCGGCTATC GATATGCCCG CCGGGCCGTC GGAAGTGCTG GTGATCGCCG ACAGCGGCGC CACCCCGGAT TTCGTCGCCT CTGACCTGCT TTCCCAGGCG GAGCACGGCC CGGATTCGCA GGTGATCCTG CTGACGCCGG ATAGCGAGAT GGCCACCCGC GTCGCGCAAG CCGTTGAGCG CCAGCTGGCC GCGCTGTCCC GTGCGGAAAC GGCCCGCGGG GCGCTCTCCG CCAGCCGCAT CATCGTGGCG CGCGATATCG CCCAGTGCGT GGAGATTTCC AACCAGTACG GTCCGGAGCA CCTGATTATT CAGACCCGCA ACGCCCGCGA ACTGGTCGAC GGCATTACCA GCGCCGGTTC GGTATTCCTC GGCGACTGGT CGCCGGAGTC CGCCGGCGAT TACGCCTCCG GCACCAACCA CGTGCTGCCG ACCTACGGCT ACACCGCCAC CTGCTCCAGC CTCGGCCTGG CGGATTTTCA GAAACGCATG ACCGTGCAGG AGCTGTCGCG CGACGGGTTT GCCGCCCTCG CCTCAACGAT TGAGATTCTG GCCGCCGCCG AGCGTCTGGA CGCCCACAAA AACGCCGTTA CGCTGCGCGT CGCAGCCCTT AAGGAGCAAG CATGA
Details for KlaeA.18181.a.B1.PW39182
PURIFICATION DATe: 1/10/2023
CONCENTRATION: 28.7mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 56
VIAL VOLUME: 110µl
PERCENT IDENTITY: 93
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS FNTIIDWNSC SAAQQQQLLM RPAISASESI SKTVAGILGN VKENGDAALR EYSAKFDKTT VTALKVSDEE IAAAGARLSD ELKQAMAVAV NNIETFHNAQ QLQVVDVETQ PGVRCQQLTR PIASVGLYIP GGSAPLFSTV LMLATPARIA GCQEVVLCSP PPIADEILYA AQLCGVRNIF NVGGAQAIAA MAFGTESVAK VAKIFGPGNA FVTEAKRQVS QRLDGAAIDM PAGPSEVLVI ADGGATPDFV ASDLLSQAEH GPDSQVILLT PDADMGSRVA AAVERQLAAL PRAETAREAL SASRIIVARD LAQCVEISNQ YGPEHLIIQT RNARELVDGI TSAGSVFLGD WSPESAGDYA SGTNHVLPTY GYTATCSSLG LADFQKRMTV QELTREGFAA LAPTIEILAA AELLTAHKNA VTLRVAALKE QA
Validated NT Sequence
atggctcacc accaccacca ccatatgagc ttcaacacaa ttatcgactg gaatagctgc agcgctgcac aacagcaaca actattaatg cgcccggcga tttcagcttc cgagagcatc agcaaaacgg tagccgggat cctcggcaac gttaaagaga atggcgatgc cgctttacgc gaatatagcg ccaaattcga taaaaccacc gtcacggcgc tcaaagtatc tgacgaagaa attgccgctg ctggcgcgcg cctcagcgac gaactcaaac aagcgatggc ggtagcggtc aacaatatcg agacctttca caacgcgcag cagttgcagg ttgtcgatgt tgaaacccag cccggcgtac gctgccagca gcttacccgc cctatcgcct ccgtcggctt atatattccg ggcggctcgg cgccgctgtt ttcaacggtg ctgatgctgg caacgccggc acgcattgcc ggctgtcagg aagtggtgct gtgttcgccg ccgccgattg ccgatgaaat tctttatgcg gcgcagctgt gcggcgtgcg taatatcttt aacgtcggcg gcgcgcaggc tatcgcggca atggcctttg gcaccgaatc ggtggcgaaa gtcgctaaaa tctttggccc tggcaacgcc ttcgtcaccg aggccaaacg ccaggtcagc cagcgcctcg acggcgcggc tatcgatatg cctgccggcc cgtcagaggt tctggtgatt gccgacggcg gcgcgacgcc ggatttcgtc gcctccgatt tactctccca ggcggaacat ggcccggatt cccaggttat cctgctgacc cccgacgccg acatgggctc ccgggtcgcc gcagcggttg aacgtcagtt ggctgccctg ccgcgtgccg aaaccgcacg tgaagcgctt tccgccagcc gtattattgt cgctcgcgat ctcgcgcaat gtgtagagat ctcaaatcag tacggaccgg agcacctgat tatccagacc cgcaacgccc gtgaactggt tgacggcatt accagcgccg gttcggtatt cctcggcgac tggtcgccag aatcagcagg tgattacgcc tccggcacca accacgtgct gccgacctac ggctacaccg cgacctgttc cagcctcggt ctggcagatt ttcaaaagcg catgaccgta caggaactga cgcgtgaagg cttcgccgcc ctcgccccga ctatcgagat cctggccgca gccgaactgc tgaccgccca caaaaacgcc gtgacgctac gcgtagcagc ccttaaggag caagcataa
Expressed Protein Sequence
MAHHHHHHMS FNTIIDWNGC SAEQQQQLLT RPAISASESI SKTVSEILNN VKDNGDAALR EYSAKFDKTD VKALKVSDEE IAAAGARLSD ELKQAMAVAV KNIETFHNAQ RLQAVDVETL PGVRCQQVTR PIASVGLYIP GGSAPLFSTV LMLATPARIA GCQQVVLCSP PPIADEILYA AQLCGVRNIF NVGGAQAIAA LAFGTESVAK VDKIFGPGNA FVTEAKRQVS QRLDGAAIDM PAGPSEVLVI ADSGATPDFV ASDLLSQAEH GPDSQVILLT PDSEMATRVA QAVERQLAAL SRAETARGAL SASRIIVARD IAQCVEISNQ YGPEHLIIQT RNARELVDGI TSAGSVFLGD WSPESAGDYA SGTNHVLPTY GYTATCSSLG LADFQKRMTV QELSRDGFAA LASTIEILAA AERLDAHKNA VTLRVAALKE QA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCT TCAACACAAT TATCGACTGG AACGGCTGTA GCGCTGAACA ACAGCAACAA CTCTTAACGC GCCCGGCCAT TTCCGCCTCC GAGAGCATCA GCAAGACGGT GTCGGAGATC CTCAATAACG TCAAAGACAA TGGCGATGCG GCTCTGCGTG AATATAGCGC GAAGTTCGAT AAAACCGACG TCAAGGCGCT GAAGGTCAGC GATGAAGAGA TTGCCGCCGC CGGCGCGCGC CTGAGCGACG AGCTCAAGCA GGCGATGGCC GTGGCGGTCA AAAATATCGA AACCTTCCAC AACGCGCAGC GGCTGCAGGC CGTCGATGTG GAAACCCTGC CGGGCGTACG CTGCCAGCAG GTGACCCGCC CTATCGCCTC CGTCGGCTTA TATATTCCCG GCGGCTCGGC GCCGCTGTTC TCCACGGTGC TGATGCTCGC CACGCCGGCG CGCATCGCGG GCTGCCAGCA GGTGGTGCTA TGCTCGCCGC CGCCGATTGC CGATGAAATC CTCTATGCCG CCCAGCTGTG CGGCGTGCGC AATATCTTTA ACGTCGGCGG CGCGCAGGCG ATTGCCGCCC TGGCGTTCGG CACCGAATCG GTGGCGAAGG TCGATAAAAT CTTTGGCCCG GGCAACGCCT TCGTGACCGA AGCCAAGCGC CAGGTCAGCC AGCGTCTTGA CGGCGCGGCT ATCGATATGC CCGCCGGGCC GTCGGAAGTG CTGGTGATCG CCGACAGCGG CGCCACCCCG GATTTCGTCG CCTCTGACCT GCTTTCCCAG GCGGAGCACG GCCCGGATTC GCAGGTGATC CTGCTGACGC CGGATAGCGA GATGGCCACC CGCGTCGCGC AAGCCGTTGA GCGCCAGCTG GCCGCGCTGT CCCGTGCGGA AACGGCCCGC GGGGCGCTCT CCGCCAGCCG CATCATCGTG GCGCGCGATA TCGCCCAGTG CGTGGAGATT TCCAACCAGT ACGGTCCGGA GCACCTGATT ATTCAGACCC GCAACGCCCG CGAACTGGTC GACGGCATTA CCAGCGCCGG TTCGGTATTC CTCGGCGACT GGTCGCCGGA GTCCGCCGGC GATTACGCCT CCGGCACCAA CCACGTGCTG CCGACCTACG GCTACACCGC CACCTGCTCC AGCCTCGGCC TGGCGGATTT TCAGAAACGC ATGACCGTGC AGGAGCTGTC GCGCGACGGG TTTGCCGCCC TCGCCTCAAC GATTGAGATT CTGGCCGCCG CCGAGCGTCT GGACGCCCAC AAAAACGCCG TTACGCTGCG CGTCGCAGCC CTTAAGGAGC AAGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT