KlaeA.17683.b

3-dehydroquinate synthase (AroB)

CENTER ID: KlaeA.17683.b
ORGANISM: Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlaeA.17683.b.B1.PW39185 Full length(KlaeA.17683.b) 1 364

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKLTVTLGE RSYPITIAAG LFNDPASFLP LKSGDQVMLV TNETLAPLYL DTVRSVLEQA GVNVDSVILP DGEQYKSLAV MDTVFTALLQ KPHGRDTTLV ALGGGVVGDL TGFAAASYQR GVRFIQVPTT LLSQVDSSVG GKTAVNHPLG KNMIGAFWQP VSVVVDLNCL KTLPKRELSS GLAEVIKYGI ILDGEFFCWL EDNIDALMAL DESAMAYCIR RCCELKAEVV AADERETGLR ALLNLGHTFG HAIEAEMGYG NWLHGEAVAA GMVMAAHTSE RLGQFSAQDT QRMIALLQRA GLPVRGPQEM SAEAYLPHMM RDKKVLAGEM RLVLPLAIGK SEVRGGVSHD VVLGAIADTQ QAQQ
NT Sequence
ATGGAGAAGC TGACTGTAAC TCTCGGGGAA CGTAGTTACC CGATTACCAT CGCGGCTGGT TTGTTTAACG ATCCAGCTTC TTTTCTGCCA CTGAAATCAG GCGACCAGGT TATGCTGGTC ACCAATGAAA CGCTGGCGCC GCTTTATCTT GATACCGTTC GCTCCGTACT GGAGCAAGCG GGTGTCAATG TCGACAGCGT GATCCTGCCG GATGGCGAGC AGTATAAAAG CCTGGCGGTG ATGGATACGG TATTTACCGC GCTGTTGCAA AAACCACATG GCCGCGACAC CACACTCGTG GCGCTCGGCG GCGGCGTGGT TGGCGATTTA ACCGGTTTTG CCGCGGCGAG CTATCAGCGC GGCGTACGTT TTATTCAAGT TCCCACCACG CTGCTTTCGC AGGTGGACTC TTCGGTTGGC GGCAAAACGG CCGTAAACCA TCCCCTCGGT AAAAATATGA TTGGCGCCTT CTGGCAGCCG GTTTCCGTGG TCGTCGACCT TAATTGTCTC AAAACATTGC CGAAGCGTGA GCTCTCCTCC GGCCTGGCTG AGGTTATTAA GTACGGCATT ATCCTCGACG GCGAGTTCTT CTGCTGGCTT GAAGACAACA TCGATGCGCT GATGGCGCTG GATGAATCAG CGATGGCGTA CTGTATTCGC CGTTGTTGTG AGCTAAAAGC CGAAGTTGTC GCTGCCGACG AGCGCGAAAC CGGGTTACGT GCTTTACTGA ATCTGGGACA TACATTTGGC CATGCTATCG AAGCTGAAAT GGGCTACGGT AACTGGCTAC ATGGCGAAGC TGTGGCGGCA GGAATGGTCA TGGCGGCGCA TACGTCCGAG CGTTTAGGGC AGTTTAGCGC GCAGGATACG CAGCGTATGA TTGCGCTGTT GCAACGTGCA GGGTTGCCGG TACGCGGGCC GCAGGAAATG TCTGCAGAAG CGTATTTGCC TCACATGATG CGTGATAAAA AGGTACTGGC AGGCGAAATG CGCCTGGTAT TACCGCTCGC TATAGGAAAG AGCGAGGTCC GCGGCGGAGT GTCGCACGAT GTAGTTCTTG GCGCGATTGC TGATACTCAG CAGGCGCAAC AATAA
Details for KlaeA.17683.b.B1.PW39185
PURIFICATION DATe: 1/11/2023
CONCENTRATION: 20.3mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 33
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME KLTVTLGERS YPITIAAGLF NDPASFLPLK SGDQVMLVTN ETLAPLYLDT VRSVLEQAGV NVDSVILPDG EQYKSLAVMD TVFTALLQKP HGRDTTLVAL GGGVVGDLTG FAAASYQRGV RFIQVPTTLL SQVDSSVGGK TAVNHPLGKN MIGAFWQPVS VVVDLNCLKT LPKRELSSGL AEVIKYGIIL DGEFFCWLED NIDALMALDE SAMAYCIRRC CELKAEVVAA DERETGLRAL LNLGHTFGHA IEAEMGYGNW LHGEAVAAGM VMAAHTSERL GQFSAQDTQR MIALLQRAGL PVRGPQEMSA EAYLPHMMRD KKVLAGEMRL VLPLAIGKSE VRGGVSHDVV LGAIADTQQA QQ
Validated NT Sequence
atggctcacc accaccacca ccatatggag aagctgactg taactctcgg ggaacgtagt tacccgatta ccatcgcggc tggtttgttt aacgatccag cttcttttct gccactgaaa tcaggcgacc aggtcatgct ggtcaccaat gaaacgctgg cgccgcttta tcttgatacc gttcgctccg tactggagca agcgggtgtc aacgttgaca gcgtgatcct gccggatggc gagcagtata aaagcctggc ggtgatggat acggtattta ccgcgctgtt gcaaaaacca catggccgcg acaccacact cgtggcgctc ggcggcggcg tggttggcga tttaaccggt tttgccgctg cgagctatca gcgcggcgta cgttttattc aagttcccac cacgctgctt tcgcaggtgg actcttcggt tggcggcaaa acggccgtaa accatcccct cggtaaaaat atgattggcg ccttctggca gccggtttcc gtggtcgtcg accttaattg tctcaaaacc ttgccgaagc gtgagctctc ctccggcctg gctgaggtta ttaagtacgg cattatcctc gacggcgagt tcttctgctg gcttgaagac aacatcgatg cgctgatggc gctggatgaa tcagcgatgg cgtactgtat tcgccgttgt tgtgagctaa aagccgaagt tgtcgctgct gacgagcgcg aaaccgggtt acgtgcttta ctgaatctgg gacatacatt tggccatgct atcgaagctg aaatgggcta cggtaactgg ctacatggcg aagctgtggc ggcaggaatg gtcatggcgg cgcatacgtc cgagcgttta gggcagttta gcgcgcagga tacgcagcgt atgattgcgc tgttgcaacg tgcagggttg ccggtacgcg ggccgcagga aatgtctgcc gaagcgtatt tgcctcacat gatgcgtgat aaaaaggtac tggcaggcga aatgcgcctg gtattaccgc tcgctatagg aaagagcgag gtccgcggcg gagtgtcgca cgatgtagtt cttggcgcga ttgctgatac tcagcaggcg caacaataa
Expressed Protein Sequence
MAHHHHHHME KLTVTLGERS YPITIAAGLF NDPASFLPLK SGDQVMLVTN ETLAPLYLDT VRSVLEQAGV NVDSVILPDG EQYKSLAVMD TVFTALLQKP HGRDTTLVAL GGGVVGDLTG FAAASYQRGV RFIQVPTTLL SQVDSSVGGK TAVNHPLGKN MIGAFWQPVS VVVDLNCLKT LPKRELSSGL AEVIKYGIIL DGEFFCWLED NIDALMALDE SAMAYCIRRC CELKAEVVAA DERETGLRAL LNLGHTFGHA IEAEMGYGNW LHGEAVAAGM VMAAHTSERL GQFSAQDTQR MIALLQRAGL PVRGPQEMSA EAYLPHMMRD KKVLAGEMRL VLPLAIGKSE VRGGVSHDVV LGAIADTQQA QQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAGA AGCTGACTGT AACTCTCGGG GAACGTAGTT ACCCGATTAC CATCGCGGCT GGTTTGTTTA ACGATCCAGC TTCTTTTCTG CCACTGAAAT CAGGCGACCA GGTTATGCTG GTCACCAATG AAACGCTGGC GCCGCTTTAT CTTGATACCG TTCGCTCCGT ACTGGAGCAA GCGGGTGTCA ATGTCGACAG CGTGATCCTG CCGGATGGCG AGCAGTATAA AAGCCTGGCG GTGATGGATA CGGTATTTAC CGCGCTGTTG CAAAAACCAC ATGGCCGCGA CACCACACTC GTGGCGCTCG GCGGCGGCGT GGTTGGCGAT TTAACCGGTT TTGCCGCGGC GAGCTATCAG CGCGGCGTAC GTTTTATTCA AGTTCCCACC ACGCTGCTTT CGCAGGTGGA CTCTTCGGTT GGCGGCAAAA CGGCCGTAAA CCATCCCCTC GGTAAAAATA TGATTGGCGC CTTCTGGCAG CCGGTTTCCG TGGTCGTCGA CCTTAATTGT CTCAAAACAT TGCCGAAGCG TGAGCTCTCC TCCGGCCTGG CTGAGGTTAT TAAGTACGGC ATTATCCTCG ACGGCGAGTT CTTCTGCTGG CTTGAAGACA ACATCGATGC GCTGATGGCG CTGGATGAAT CAGCGATGGC GTACTGTATT CGCCGTTGTT GTGAGCTAAA AGCCGAAGTT GTCGCTGCCG ACGAGCGCGA AACCGGGTTA CGTGCTTTAC TGAATCTGGG ACATACATTT GGCCATGCTA TCGAAGCTGA AATGGGCTAC GGTAACTGGC TACATGGCGA AGCTGTGGCG GCAGGAATGG TCATGGCGGC GCATACGTCC GAGCGTTTAG GGCAGTTTAG CGCGCAGGAT ACGCAGCGTA TGATTGCGCT GTTGCAACGT GCAGGGTTGC CGGTACGCGG GCCGCAGGAA ATGTCTGCAG AAGCGTATTT GCCTCACATG ATGCGTGATA AAAAGGTACT GGCAGGCGAA ATGCGCCTGG TATTACCGCT CGCTATAGGA AAGAGCGAGG TCCGCGGCGG AGTGTCGCAC GATGTAGTTC TTGGCGCGAT TGCTGATACT CAGCAGGCGC AACAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT