KlaeA.17333.b
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase
| CENTER ID: | KlaeA.17333.b |
| ORGANISM: | Klebsiella aerogenes KCTC 2190 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlaeA.17333.b.B1.PW39179 | Full length(KlaeA.17333.b) | 1 | 379 |
Structures
8SNG
DEPOSITED: 4/27/2023
DETERMINATION: XRay
CLONE: KlaeA.17333.b.B1.GE44863
PROTEIN: KlaeA.17333.b.B1.PW39179
8SNJ
DEPOSITED: 4/27/2023
DETERMINATION: XRay
CLONE: KlaeA.17333.b.B1.GE44863
PROTEIN: KlaeA.17333.b.B1.PW39179
8SU6
DEPOSITED: 5/11/2023
DETERMINATION: XRay
CLONE: KlaeA.17333.b.B1.GE44863
PROTEIN: KlaeA.17333.b.B1.PW39179
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
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AA Sequence
MSDFLPFSRP SMGDAELAAL REVLQSGWIT TGPKNQALEE AFCTLTGNRH AIAVSSATGG MHVTLMGLGI GAGDEVITPS QTWVSTLNMI CLLGATPVMI DVDPDNLMIT PEAVEAAITP RTKAIIPVHY AGAPADIDAI RAIGERHAIP VIEDAAHAAG TYYKGRHVGW RGTAIFSFHA IKNMTCAEGG LVVTDDDELA ARIRSLKFHG LGVDAYDRQT LGRAPQAEVI SPGFKYNLAD INAALALVQL DKLAHANQRR AAIAQRYLRE LADTPFRPLV APSWEHQHAW HLFIIRVDEA ACGISRDALM ERLKAMGIGT GLHFRAAHTQ KYYRERFPEV SLPHTEWNSA RICSIPLFPD MTDDDVTRVI SALHQLSGR
NT Sequence
ATGAGCGATT TTTTGCCGTT TTCGCGCCCG TCGATGGGCG ACGCAGAGCT GGCTGCGCTG CGTGAAGTTT TGCAATCGGG CTGGATCACC ACTGGACCGA AAAATCAGGC GCTGGAAGAG GCGTTCTGCA CGTTAACCGG CAACCGTCAC GCCATCGCCG TCAGTTCGGC GACCGGCGGC ATGCACGTCA CGCTGATGGG GCTGGGTATC GGTGCGGGCG ATGAAGTGAT CACCCCTTCC CAGACCTGGG TCTCCACGCT GAACATGATC TGCCTGCTCG GCGCGACCCC GGTGATGATC GATGTCGATC CCGATAACCT GATGATTACC CCCGAAGCGG TTGAAGCGGC AATCACCCCG CGAACCAAAG CCATTATCCC TGTGCACTAT GCTGGCGCAC CGGCGGATAT TGACGCCATT CGCGCGATTG GCGAGCGTCA CGCTATTCCG GTGATTGAAG ATGCCGCCCA CGCGGCGGGG ACGTACTACA AAGGCCGCCA CGTCGGGTGG CGCGGTACCG CCATTTTCTC ATTCCATGCG ATCAAGAATA TGACCTGCGC CGAAGGCGGG CTGGTGGTGA CCGACGATGA CGAACTGGCC GCGCGCATTC GCAGCCTGAA GTTCCACGGC CTCGGCGTTG ACGCTTATGA CCGCCAGACC CTGGGCCGCG CCCCGCAGGC GGAGGTGATT TCGCCGGGCT TTAAATACAA TCTCGCGGAT ATCAACGCCG CGCTGGCGCT GGTGCAGCTG GACAAGCTCG CCCACGCCAA TCAGCGCCGC GCCGCCATTG CGCAACGCTA TCTGCGAGAG CTGGCGGATA CGCCCTTCAG GCCGCTGGTG GCGCCGTCGT GGGAGCATCA GCACGCCTGG CACCTGTTTA TTATCCGCGT TGACGAAGCC GCCTGCGGCA TCAGCCGCGA TGCGCTGATG GAAAGACTGA AGGCCATGGG CATTGGCACC GGCCTGCACT TCCGCGCGGC CCATACGCAA AAGTATTACC GCGAGCGTTT CCCTGAAGTT TCATTACCGC ATACGGAATG GAACAGCGCG CGCATTTGTT CCATTCCGTT GTTCCCGGAT ATGACCGATG ACGATGTCAC TCGGGTCATT TCCGCACTGC ACCAGCTGTC AGGACGTTGA
Details for KlaeA.17333.b.B1.PW39179
| PURIFICATION DATe: | 1/9/2023 |
| CONCENTRATION: | 27.29mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 53 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS DFLPFSRPSM GDAELAALRE VLQSGWITTG PKNQALEEAF CQLTGNRHAI AVSSATGGMH VTLMAMGIGP GDEVITPSQT WVSTLNMICL LGATPVMIDV DHDNLMITPE AVEAAITSRT KAIIPVHYAG APADIDAIRA VGERHGIPVI EDAAHAAGTH YKGRHVGWRG TAIFSFHAIK NMTCAEGGLI VTDDDELASR IRSLKFHGLG VDAYDRQTHG RAPQAEVITP GFKYNLADIN AALALVQLDK LAQANQRRAE IAQRYLRELA DTPFKPLTIP AWDHQHAWHL FIIRVDEAAC GISRDVLMEK LKAMGIGTGL HFRAAHTQKY YRERFPEVSL PNTEWNSARI CSIPLFPDMT DDDVTRVITA LHQLSGR
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gattttttgc cgttttcgcg cccgtcgatg ggcgacgcag agctggctgc gctgcgtgaa gttttgcaat caggatggat cactaccggt cctaaaaacc aggcgctgga agaggcgttt tgccaactga ccggcaaccg ccacgccatc gccgtcagct ccgctaccgg cggtatgcac gttacgctaa tggcgatggg cattggccct ggcgacgaag tgatcacccc ttcgcaaacc tgggtgtcga cgctgaacat gatctgtctg ctgggcgcta cgccggtgat gatcgatgtc gaccacgaca atctgatgat tacccctgag gcggtggaag cggcgatcac ttcgcggacg aaagcgatta tacctgtcca ctacgcgggc gcgcctgcgg atatcgatgc gatccgcgcc gttggcgagc gccacggtat accggtaatc gaagatgccg cgcatgccgc gggaacgcac tataaaggcc gccacgtcgg ctggcgcggc accgcgattt tctcgttcca cgcgatcaaa aatatgacct gcgccgaagg ggggcttatc gtcaccgatg atgacgagct ggcttcccgc atccgcagcc tcaaattcca cggcctgggc gttgacgcct acgaccgcca gacccacggc cgcgcgccgc aggcggaagt gattaccccc ggcttcaaat acaatctggc cgatatcaac gccgcgctgg cgctggtgca gctggataag ctggcgcagg cgaatcaacg tcgcgctgaa atcgcccaac gctacctgcg cgagctggcg gataccccct ttaagccgct gaccattccg gcatgggatc accaacacgc ctggcacctg tttattatcc gcgttgatga agcggcctgc ggcatcagcc gcgatgtcct gatggaaaaa ctcaaagcca tgggcatagg caccggtctg cacttccgcg cggcccatac gcaaaagtat taccgcgagc gttttcctga agtttcgtta ccgaataccg aatggaacag cgcgcgcatt tgttctatcc cgctgttccc ggatatgacc gacgacgatg tcacccgcgt cattaccgcg ctgcaccagc tgtcaggacg ttaa
Expressed Protein Sequence
MAHHHHHHMS DFLPFSRPSM GDAELAALRE VLQSGWITTG PKNQALEEAF CTLTGNRHAI AVSSATGGMH VTLMGLGIGA GDEVITPSQT WVSTLNMICL LGATPVMIDV DPDNLMITPE AVEAAITPRT KAIIPVHYAG APADIDAIRA IGERHAIPVI EDAAHAAGTY YKGRHVGWRG TAIFSFHAIK NMTCAEGGLV VTDDDELAAR IRSLKFHGLG VDAYDRQTLG RAPQAEVISP GFKYNLADIN AALALVQLDK LAHANQRRAA IAQRYLRELA DTPFRPLVAP SWEHQHAWHL FIIRVDEAAC GISRDALMER LKAMGIGTGL HFRAAHTQKY YRERFPEVSL PHTEWNSARI CSIPLFPDMT DDDVTRVISA LHQLSGR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ATTTTTTGCC GTTTTCGCGC CCGTCGATGG GCGACGCAGA GCTGGCTGCG CTGCGTGAAG TTTTGCAATC GGGCTGGATC ACCACTGGAC CGAAAAATCA GGCGCTGGAA GAGGCGTTCT GCACGTTAAC CGGCAACCGT CACGCCATCG CCGTCAGTTC GGCGACCGGC GGCATGCACG TCACGCTGAT GGGGCTGGGT ATCGGTGCGG GCGATGAAGT GATCACCCCT TCCCAGACCT GGGTCTCCAC GCTGAACATG ATCTGCCTGC TCGGCGCGAC CCCGGTGATG ATCGATGTCG ATCCCGATAA CCTGATGATT ACCCCCGAAG CGGTTGAAGC GGCAATCACC CCGCGAACCA AAGCCATTAT CCCTGTGCAC TATGCTGGCG CACCGGCGGA TATTGACGCC ATTCGCGCGA TTGGCGAGCG TCACGCTATT CCGGTGATTG AAGATGCCGC CCACGCGGCG GGGACGTACT ACAAAGGCCG CCACGTCGGG TGGCGCGGTA CCGCCATTTT CTCATTCCAT GCGATCAAGA ATATGACCTG CGCCGAAGGC GGGCTGGTGG TGACCGACGA TGACGAACTG GCCGCGCGCA TTCGCAGCCT GAAGTTCCAC GGCCTCGGCG TTGACGCTTA TGACCGCCAG ACCCTGGGCC GCGCCCCGCA GGCGGAGGTG ATTTCGCCGG GCTTTAAATA CAATCTCGCG GATATCAACG CCGCGCTGGC GCTGGTGCAG CTGGACAAGC TCGCCCACGC CAATCAGCGC CGCGCCGCCA TTGCGCAACG CTATCTGCGA GAGCTGGCGG ATACGCCCTT CAGGCCGCTG GTGGCGCCGT CGTGGGAGCA TCAGCACGCC TGGCACCTGT TTATTATCCG CGTTGACGAA GCCGCCTGCG GCATCAGCCG CGATGCGCTG ATGGAAAGAC TGAAGGCCAT GGGCATTGGC ACCGGCCTGC ACTTCCGCGC GGCCCATACG CAAAAGTATT ACCGCGAGCG TTTCCCTGAA GTTTCATTAC CGCATACGGA ATGGAACAGC GCGCGCATTT GTTCCATTCC GTTGTTCCCG GATATGACCG ATGACGATGT CACTCGGGTC ATTTCCGCAC TGCACCAGCT GTCAGGACGT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT