SSGCID: Target Page

KlaeA.17231.a

Extracellular deoxyribonuclease Dns (EC 3.1.21.-)

CENTER ID:	KlaeA.17231.a
ORGANISM:	Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlaeA.17231.a.B1.GE44532	Full length(KlaeA.17231.a)		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1028307.3.peg.134
UniProt:	A0A0H3FKZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHNQAPIQRR KSKRIYVGNV PIGDGAPIAV QSMTNTRTTD VAATVNQIKA LERVGADIVR VSVPTMDAAE AFKLIKQQVN VPLVADIHFD YRIALKVAEY GVDCLRINPG NIGNEERIRM VVDCARDKNI PIRIGVNAGS LEKDLQEKYG EPTPQALLES AMRHVDHLDR LNFDQFKVSV KASDVFLAVE SYRLLAKQID QPLHLGITEA GGARSGAVKS AIGLGLLLSE GIGDTLRVSL AADPVEEIKV GFDILKSLRI RSRGINFIAC PTCSRQEFDV IGTVNALEQR LEDIITPMDV SIIGCVVNGP GEALVSTLGV TGGNKKSGLY EDGVRKDRLD NNDMIDQLEA RIRAKATMLD EARRIEVQQV EAK

NT Sequence

ATGCATAACC AGGCTCCAAT TCAACGTAGA AAGTCCAAAC GCATTTACGT TGGTAATGTG CCGATTGGCG ATGGGGCTCC CATCGCCGTA CAGTCGATGA CCAATACTCG CACCACCGAT GTGGCCGCGA CGGTAAATCA AATCAAAGCG CTGGAGCGCG TAGGCGCGGA CATCGTCCGC GTTTCCGTCC CGACGATGGA CGCAGCGGAA GCTTTCAAGC TCATCAAACA GCAGGTCAAC GTCCCGCTGG TCGCCGATAT CCACTTTGAC TACCGTATCG CGCTGAAAGT CGCGGAATAT GGCGTTGATT GCCTGCGCAT CAACCCTGGC AACATTGGTA ACGAAGAGCG TATCCGCATG GTTGTGGACT GCGCGCGCGA CAAAAACATT CCTATCCGCA TCGGCGTCAA CGCCGGTTCG CTGGAAAAAG ATCTGCAGGA AAAATACGGC GAGCCGACGC CGCAGGCGCT GTTAGAGTCC GCCATGCGTC ACGTCGATCA TCTCGATCGT TTGAACTTCG ATCAGTTCAA AGTTAGCGTA AAAGCCTCAG ATGTCTTCCT CGCTGTTGAA TCCTATCGTC TGTTGGCGAA GCAGATCGAT CAACCGCTGC ATCTCGGGAT CACTGAAGCC GGCGGCGCGC GTAGCGGCGC GGTAAAATCC GCGATCGGTT TAGGTCTGCT GCTGTCTGAA GGGATCGGCG ATACCCTGCG CGTGTCGCTG GCCGCCGACC CGGTGGAAGA GATCAAAGTT GGCTTCGATA TCCTGAAGTC GCTGCGCATC CGTTCTCGCG GTATTAACTT TATCGCTTGC CCGACCTGCT CGCGTCAGGA GTTTGACGTC ATCGGTACGG TGAACGCGCT GGAGCAGCGT CTGGAAGATA TCATCACTCC GATGGATGTT TCGATCATCG GCTGTGTGGT TAACGGCCCT GGCGAAGCGC TGGTTTCCAC TCTCGGCGTC ACTGGCGGCA ACAAGAAAAG CGGCCTGTAC GAGGACGGCG TTCGCAAAGA CCGACTGGAC AACAACGACA TGATCGACCA GCTGGAAGCG CGTATCCGTG CGAAAGCCAC CATGCTGGAT GAAGCGCGTC GTATTGAAGT TCAGCAGGTT GAAGCAAAAT AA

Details for KlaeA.17231.a.B1.GE44532

HARVESTED ON:	11/2/2021
SEQUENCED ON:	11/10/2021
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMH NQAPIQRRKS KRIYVGNVPI GDGAPIAVQS MTNTRTTDVA ATVNQIKALE RVGADIVRVS VPTMDAAEAF KLIKQQVNVP LVADIHFDYR IALKVAEYGV DCLRINPGNI GNEERIRMVV DCARDKNIPI RIGVNAGSLE KDLQEKYGEP TPQALLESAM RHVDHLDRLN FDQFKVSVKA SDVFLAVESY RLLAKQIDQP LHLGITEAGG ARSGAVKSAI GLGLLLSEGI GDTLRVSLAA DPVEEIKVGF DILKSLRIRS RGINFIACPT CSRQEFDVIG TVNALEQRLE DIITPMDVSI IGCVVNGPGE ALVSTLGVTG GNKKSGLYED GVRKDRLDNN DMIDQLEARI RAKATMLDEA RRI

Validated NT Sequence

tcaatacgac gcgcttcatc cagcatggtg gctttcgcac ggatacgcgc ttccagctgg tcgatcatgt cgttgttgtc cagtcggtct ttgcgaacgc cgtcctcgta caggccgctt ttcttgttgc cgccagtgac gccgagagtg gaaaccagcg cttcgccagg gccgttaacc acacagccga tgatcgaaac atccatcgga gtgatgatat cttccagacg ctgctccagc gcgttcaccg taccgatgac gtcaaactcc tgacgcgagc aggtcgggca agcgataaag ttaataccgc gagaacggat gcgcagcgac ttcaggatat cgaagccaac tttgatctct tccaccgggt cggcggccag cgacacgcgc agggtatcgc cgatcccttc agacagcagc agacctaaac cgatcgcgga ttttaccgcg ccgctacgcg cgccgccggc ttcagtgatc ccgagatgca gcggttgatc gatctgcttc gccaacagac gataggattc aacagcgagg aagacatctg aggcttttac gctaactttg aactgatcga agttcaaacg atcgagatga tcgacgtgac gcatggcgga ctctaacagc gcctgcggcg tcggctcgcc gtatttttcc tgcagatctt tttccagcga accggcgttg acgccgatgc ggataggaat gtttttgtcg cgcgcgcagt ccacaaccat gcggatacgc tcttcgttac caatgttgcc agggttgatg cgcaggcaat caacgccata ttccgcgact ttcagcgcga tacggtagtc aaagtggata tcggcgacca gcgggacgtt gacctgctgt ttgatgagct tgaaagcttc cgctgcgtcc atcgtcggga cggaaacgcg gacgatgtcc gcgcctacgc gctccagcgc tttgatttga tttaccgtcg cggccacatc ggtggtgcga gtattggtca tcgactgtac ggcgatggga gccccatcgc caatcggcac attaccaacg taaatgcgtt tggactttct acgttgaatt ggagcctggt tatgcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata accaggctcc aattcaacgt agaaagtcca aacgcattta cgttggtaat gtgccgattg gcgatggggc tcccatcgcc gtacagtcga tgaccaatac tcgcaccacc gatgtggccg cgacggtaaa tcaaatcaaa gcgctggagc gcgtaggcgc ggacatcgtc cgcgtttccg tcccgacgat ggacgcagcg gaagctttca agctcatcaa acagcaggtc aacgtcccgc tggtcgccga tatccacttt gactaccgta tcgcgctgaa agtcgcggaa tatggcgttg attgcctgcg catcaaccct ggcaacattg gtaacgaaga gcgtatccgc atggttgtgg actgcgcgcg cgacaaaaac attcctatcc gcatcggcgt caacgccggt tcgctggaaa aagatctgca ggaaaaatac ggcgagccga cgccgcaggc gctgttagag tccgccatgc gtcacgtcga tcatctcgat cgtttgaact tcgatcagtt caaagttagc gtaaaagcct cagatgtctt cctcgctgtt gaatcctatc gtctgttggc gaagcagatc gatcaaccgc tgcatctcgg gatcactgaa gccggcggcg cgcgtagcgg cgcggtaaaa tccgcgatcg gtttaggtct gctgctgtct gaagggatcg gcgataccct gcgcgtgtcg ctggccgccg acccggtgga agagatcaaa gttggcttcg atatcctgaa gtcgctgcgc atccgttctc gcggtattaa ctttatcgct tgcccgacct gctcgcgtca ggagtttgac gtcatcggta cggtgaacgc gctggagcag cgtctggaag atatcatcac tccgatggat gtttcgatca tcggctgtgt ggttaacggc cctggcgaag cgctggtttc cactctcggc gtcactggcg gcaacaagaa aagcggcctg tacgaggacg gcgttcgcaa agaccgactg gacaacaacg acatgatcga ccagctggaa gcgcgtatcc gtgcgaaagc caccatgctg gatgaagcgc gtcgtattga agttcagcag gttgaagcaa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat