KlaeA.17085.a

Coproporphyrinogen-III oxidase

CENTER ID:	KlaeA.17085.a
ORGANISM:	Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlaeA.17085.a.B1.PW39170	Full length(KlaeA.17085.a)		1	299

Structures

8T7W

DEPOSITED: 6/21/2023

DETERMINATION: XRay

Diffraction Image

CLONE: KlaeA.17085.a.B1.GE44860

PROTEIN: KlaeA.17085.a.B1.PW39170

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPDVQQVKA FLLQLQDAIC AKLSAVDGKD FVEDSWQREG GGGGRSRVLR DGGVFEQAGV NFSHVHGDAM PASATAHRPE LAGRSFEAMG VSLVVHPRNP YVPTSHANVR FFIAEKPGAD PVWWFGGGFD LTPYYGFEED AIHWHRTARD LCLPYGDEVY PRYKKWCDDY FFLKHRNEQR GIGGLFFDDL NTPDFDHCFA FMQAVGNGFT DAYLPIVERR KTTPYGERER DFQLYRRGRY VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYHP QEGSPEAALS EFIKVKEWI

NT Sequence

ATGAAACCCG ATGTACAGCA GGTCAAAGCC TTCCTTCTTC AGCTGCAGGA TGCCATTTGC GCCAAACTCT CCGCCGTTGA CGGCAAGGAT TTTGTTGAAG ATAGCTGGCA GCGCGAAGGC GGCGGCGGCG GACGCAGCCG GGTTTTACGC GACGGCGGCG TCTTTGAGCA GGCCGGGGTT AACTTCTCTC ACGTCCATGG CGATGCGATG CCCGCCTCAG CTACCGCCCA CCGCCCGGAA CTGGCCGGGC GCAGCTTTGA GGCGATGGGC GTTTCGCTGG TGGTGCATCC GCGCAACCCC TATGTGCCGA CCAGCCACGC TAACGTTCGC TTTTTTATTG CCGAGAAGCC CGGCGCCGAC CCGGTGTGGT GGTTCGGCGG CGGGTTTGAT CTAACGCCTT ATTATGGGTT TGAAGAAGAC GCCATTCACT GGCATCGTAC TGCCCGCGAT TTATGCCTGC CGTACGGCGA CGAGGTCTAT CCGCGCTATA AAAAGTGGTG CGATGATTAC TTCTTCCTTA AGCACCGTAA CGAACAGCGC GGCATTGGCG GTCTGTTCTT TGATGATTTA AATACTCCGG ACTTCGACCA CTGCTTCGCC TTTATGCAGG CAGTGGGCAA CGGCTTTACC GACGCTTACC TGCCGATCGT TGAGCGCCGA AAAACCACGC CTTACGGCGA ACGCGAACGC GATTTCCAGC TTTATCGCCG CGGGCGCTAT GTGGAATTCA ATCTGGTGTG GGATCGCGGC ACGCTGTTCG GCCTGCAGAC CGGCGGGCGT ACGGAGTCTA TTCTGATGTC GATGCCGCCG CTGGTGCGCT GGGAGTATGA CTACCATCCG CAAGAAGGCA GCCCGGAAGC CGCATTGAGC GAGTTTATTA AAGTCAAAGA GTGGATCTGA

Details for KlaeA.17085.a.B1.PW39170

PURIFICATION DATe:	12/14/2022
CONCENTRATION:	43.36mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	35
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMK PDVQQVKAFL LQLQDAICAK LSAVDGKDFV EDSWQREGGG GGRSRVLRNG GIFEQAGVNF SHVHGDAMPA SATAHRPELA GRSFEAMGVS LVVHPRNPYV PTSHANVRFF IAEKPGADPV WWFGGGFDLT PYYGFEEDAI HWHRTARDLC LPYGDEVYPR YKKWCDDYFF LKHRNEQRGI GGLFFDDLNT PDFDHCFAFM QAVGNGFTDA YLPIVERRKT TPYGERERDF QLYRRGRYVE FNLVWDRGTL FGLQTGGRTE SILMSMPPLV RWEYDYHPQE GSPEAALSEF IKVKEWI

Validated NT Sequence

atggctcacc accaccacca ccatatgaaa cccgatgtac agcaggtcaa agccttcctt cttcagctgc aggatgccat ttgcgccaaa ctctccgccg ttgacggcaa ggattttgtt gaagatagct ggcagcgcga aggcggcggc ggcggacgca gccgggtttt acgtaacggc ggcatctttg agcaggccgg ggttaacttt tctcacgtcc acggcgatgc gatgcccgcc tcggctaccg cccaccgccc ggaactggcc gggcgcagct ttgaagcaat gggggtttcg ctggtggtgc atccgcgcaa cccctatgtg ccgaccagcc acgctaacgt tcgctttttt attgccgaga agcccggcgc cgacccggtg tggtggttcg gcggcgggtt tgatctaacg ccttattatg ggtttgaaga agacgccatt cactggcatc gtactgcccg cgatttatgc ctgccgtacg gcgacgaggt ctatccgcgc tataaaaagt ggtgcgatga ttacttcttc cttaagcacc gtaacgaaca gcgcggcatt ggcggcctgt tcttcgatga tttaaatacc ccggacttcg accactgctt cgcctttatg caggcggtgg gcaacggctt taccgacgct tacctgccga tcgttgagcg tcgaaaaacc acgccttacg gtgagcgcga acgcgatttc cagctttatc gccgcgggcg ctatgtggaa ttcaatctgg tgtgggatcg cggcacgctg ttcggcctgc agaccggtgg gcgtacggag tctattctga tgtcgatgcc gccgctggtg cgctgggagt atgactacca tccgcaagaa ggcagcccgg aagccgcatt gagcgaattt attaaagtca aagagtggat ctaa

Expressed Protein Sequence

MAHHHHHHMK PDVQQVKAFL LQLQDAICAK LSAVDGKDFV EDSWQREGGG GGRSRVLRDG GVFEQAGVNF SHVHGDAMPA SATAHRPELA GRSFEAMGVS LVVHPRNPYV PTSHANVRFF IAEKPGADPV WWFGGGFDLT PYYGFEEDAI HWHRTARDLC LPYGDEVYPR YKKWCDDYFF LKHRNEQRGI GGLFFDDLNT PDFDHCFAFM QAVGNGFTDA YLPIVERRKT TPYGERERDF QLYRRGRYVE FNLVWDRGTL FGLQTGGRTE SILMSMPPLV RWEYDYHPQE GSPEAALSEF IKVKEWI

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC CCGATGTACA GCAGGTCAAA GCCTTCCTTC TTCAGCTGCA GGATGCCATT TGCGCCAAAC TCTCCGCCGT TGACGGCAAG GATTTTGTTG AAGATAGCTG GCAGCGCGAA GGCGGCGGCG GCGGACGCAG CCGGGTTTTA CGCGACGGCG GCGTCTTTGA GCAGGCCGGG GTTAACTTCT CTCACGTCCA TGGCGATGCG ATGCCCGCCT CAGCTACCGC CCACCGCCCG GAACTGGCCG GGCGCAGCTT TGAGGCGATG GGCGTTTCGC TGGTGGTGCA TCCGCGCAAC CCCTATGTGC CGACCAGCCA CGCTAACGTT CGCTTTTTTA TTGCCGAGAA GCCCGGCGCC GACCCGGTGT GGTGGTTCGG CGGCGGGTTT GATCTAACGC CTTATTATGG GTTTGAAGAA GACGCCATTC ACTGGCATCG TACTGCCCGC GATTTATGCC TGCCGTACGG CGACGAGGTC TATCCGCGCT ATAAAAAGTG GTGCGATGAT TACTTCTTCC TTAAGCACCG TAACGAACAG CGCGGCATTG GCGGTCTGTT CTTTGATGAT TTAAATACTC CGGACTTCGA CCACTGCTTC GCCTTTATGC AGGCAGTGGG CAACGGCTTT ACCGACGCTT ACCTGCCGAT CGTTGAGCGC CGAAAAACCA CGCCTTACGG CGAACGCGAA CGCGATTTCC AGCTTTATCG CCGCGGGCGC TATGTGGAAT TCAATCTGGT GTGGGATCGC GGCACGCTGT TCGGCCTGCA GACCGGCGGG CGTACGGAGT CTATTCTGAT GTCGATGCCG CCGCTGGTGC GCTGGGAGTA TGACTACCAT CCGCAAGAAG GCAGCCCGGA AGCCGCATTG AGCGAGTTTA TTAAAGTCAA AGAGTGGATC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT