KlaeA.01628.a
Thymidylate kinase (Tmk)
| CENTER ID: | KlaeA.01628.a |
| ORGANISM: | Klebsiella aerogenes KCTC 2190 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlaeA.01628.a.B1.PW39187 | Full length(KlaeA.01628.a) | 1 | 213 |
Structures
9O57
DEPOSITED: 4/9/2025
DETERMINATION: XRay
CLONE: KlaeA.01628.a.B1.GE44859
PROTEIN: KlaeA.01628.a.B1.PW39187
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| RefSeq: | WP_020079143.1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSNYIVIEG LEGAGKTTAR ECVVETLKQL GVSDMVFTRE PGGTILAEKL RSLVLDIQST GDEVINDKAE VLMFYAARVQ LVETVIKPAL ARGQWVIGDR HDLSTQAYQG GGRGIDQTML ATLRDAVLGD FRPHLTLYLD VTPEVGLKRA RARGELDRIE QESLDFFNRT RARYLELAAQ DPTIRTVDAT QPLEAVERDI RAVVNRWVAE QLP
NT Sequence
ATGCGCAGTA ACTATATCGT CATCGAGGGC CTGGAGGGCG CAGGGAAAAC CACCGCGCGT GAATGTGTGG TAGAGACGCT AAAACAGCTG GGCGTTAGCG ATATGGTGTT TACCCGCGAA CCGGGCGGCA CCATTCTGGC CGAGAAGCTG CGCAGCCTGG TGCTGGATAT TCAGTCCACC GGCGATGAAG TGATTAATGA TAAAGCGGAA GTGCTGATGT TTTACGCGGC GCGCGTACAG CTGGTAGAGA CGGTGATTAA ACCCGCTCTG GCGCGCGGTC AATGGGTCAT TGGCGATCGT CACGATCTCT CTACCCAGGC CTATCAGGGC GGCGGTCGCG GTATCGACCA GACGATGCTG GCGACCCTGC GTGACGCGGT ACTGGGAGAC TTTCGTCCCC ATCTGACGCT TTATCTTGAT GTGACTCCGG AGGTGGGCCT GAAGCGCGCC CGCGCCCGCG GCGAACTTGA CCGAATTGAG CAGGAGTCGC TGGATTTCTT TAATCGAACT CGTGCGCGCT ACCTTGAACT GGCGGCGCAG GATCCCACCA TCCGCACCGT TGACGCCACC CAGCCGCTGG AGGCCGTCGA GCGCGACATC CGTGCGGTGG TGAATCGGTG GGTTGCGGAG CAACTCCCAT GA
Details for KlaeA.01628.a.B1.PW39187
| PURIFICATION DATe: | 1/11/2023 |
| CONCENTRATION: | 19.7mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 33 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR SNYIVIEGLE GAGKTTARNL VVEALEQLGI RDMVFTREPG GTILAEKLRS LVLDIQSTGD EVINDKAEVL MFYAARVQLV ETVIKPALAR GQWVIGDRHD LSTQAYQGGG RGIDQGMLAT LRDAVLGDFR PDLTLYLDVT PEVGLKRARA RGDLDRIEQE SMNFFNRTRA RYLELAAQDP TIRTVDATQP LEAVERDIRA VITQWVAEQL P
Validated NT Sequence
atggctcacc accaccacca ccatatgcgc agtaactata tcgttatcga aggcctggaa ggggcgggga aaaccaccgc acgcaatctg gtcgttgagg ctctggaaca gttgggtatc cgcgatatgg tgtttacccg tgaaccgggc ggtaccatcc tggctgaaaa gctgcgcagc ctggtgctgg atatccagtc taccggcgat gaagtcatta acgataaagc tgaagtgctg atgttttacg ccgcccgcgt acagctggtg gaaacggtga ttaagcctgc tctggctcgc ggtcaatggg tcattggcga ccgtcacgat ctctcaactc aggcctacca gggcgggggg cgcggtatcg atcaggggat gctggcgacg ctacgcgatg cggtgctcgg tgattttcgc cctgatttaa cactctatct tgacgtgacg ccggaagtcg gtctcaagcg cgcccgcgcg cgaggcgatc tggatcgtat tgagcaggag tcgatgaatt tctttaatcg tacccgcgcg cgctaccttg aactggcggc tcaggatccg actatccgca cggtggatgc gacgcagcct ctggaagcgg ttgagcgcga cattcgcgcc gttatcactc aatgggttgc ggagcaactc ccataa
Expressed Protein Sequence
MAHHHHHHMR SNYIVIEGLE GAGKTTAREC VVETLKQLGV SDMVFTREPG GTILAEKLRS LVLDIQSTGD EVINDKAEVL MFYAARVQLV ETVIKPALAR GQWVIGDRHD LSTQAYQGGG RGIDQTMLAT LRDAVLGDFR PHLTLYLDVT PEVGLKRARA RGELDRIEQE SLDFFNRTRA RYLELAAQDP TIRTVDATQP LEAVERDIRA VVNRWVAEQL P
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCA GTAACTATAT CGTCATCGAG GGCCTGGAGG GCGCAGGGAA AACCACCGCG CGTGAATGTG TGGTAGAGAC GCTAAAACAG CTGGGCGTTA GCGATATGGT GTTTACCCGC GAACCGGGCG GCACCATTCT GGCCGAGAAG CTGCGCAGCC TGGTGCTGGA TATTCAGTCC ACCGGCGATG AAGTGATTAA TGATAAAGCG GAAGTGCTGA TGTTTTACGC GGCGCGCGTA CAGCTGGTAG AGACGGTGAT TAAACCCGCT CTGGCGCGCG GTCAATGGGT CATTGGCGAT CGTCACGATC TCTCTACCCA GGCCTATCAG GGCGGCGGTC GCGGTATCGA CCAGACGATG CTGGCGACCC TGCGTGACGC GGTACTGGGA GACTTTCGTC CCCATCTGAC GCTTTATCTT GATGTGACTC CGGAGGTGGG CCTGAAGCGC GCCCGCGCCC GCGGCGAACT TGACCGAATT GAGCAGGAGT CGCTGGATTT CTTTAATCGA ACTCGTGCGC GCTACCTTGA ACTGGCGGCG CAGGATCCCA CCATCCGCAC CGTTGACGCC ACCCAGCCGC TGGAGGCCGT CGAGCGCGAC ATCCGTGCGG TGGTGAATCG GTGGGTTGCG GAGCAACTCC CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT