KlaeA.01229.a
2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase (EC 3.1.3.77)
| CENTER ID: | KlaeA.01229.a |
| ORGANISM: | Klebsiella aerogenes KCTC 2190 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlaeA.01229.a.B1.GE44531 | Full length(KlaeA.01229.a) | 1 | 245 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
8DOP
DEPOSITED: 7/14/2022
DETERMINATION: XRay
CLONE: KlaeA.01229.a.B1.GE44531
PROTEIN: KlaeA.01229.a.B1.PW39096
8DOQ
DEPOSITED: 7/14/2022
DETERMINATION: XRay
CLONE: KlaeA.01229.a.B1.GE44531
PROTEIN: KlaeA.01229.a.B1.PW39096
8EK7
DEPOSITED: 9/20/2022
DETERMINATION: XRay
CLONE: KlaeA.01229.a.B1.GE44531
PROTEIN: KlaeA.01229.a.B1.PW39096
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|571.62.peg.3915 |
| UniProt: | A0A0G3SAC1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSTATSSSR VVTSSPIVVA LDYDNRDKAL AFVDRIDPRD CRLKVGKEMF TLLGPQFVRD LHQRGFEIFL DLKFHDIPNT TARAVAAAAE LGVWMVNVHA SGGARMMTAA REALLPFGKD APLLIAVTVL TSMEASDLQD LGITLSPADH AAKLAALTQR CGLDGVVCSA QEAVRFKQEL GQAFKLVTPG IRPQGSDAGD QRRIMTPEQA QAAGVDYMVI GRPVTQSADP AATLRGINAS LNKGV
NT Sequence
ATGACGTCTA CTGCAACATC TTCTTCCCGC GTTGTCACAT CCTCTCCCAT CGTCGTTGCT CTCGATTATG ATAACCGTGA TAAAGCGCTG GCGTTTGTCG ACCGTATCGA CCCGCGCGAC TGCCGGCTCA AGGTTGGTAA AGAGATGTTT ACCCTGCTGG GGCCGCAGTT TGTTCGCGAC CTGCACCAGC GCGGTTTTGA GATTTTCCTC GATCTAAAGT TTCACGATAT CCCGAATACC ACGGCGCGTG CGGTCGCCGC AGCGGCAGAG CTTGGCGTCT GGATGGTTAA CGTTCATGCC TCCGGCGGCG CGCGTATGAT GACCGCCGCA CGTGAGGCCC TGCTGCCTTT CGGAAAAGAT GCGCCGCTAT TAATTGCGGT AACGGTGTTA ACCAGCATGG AAGCCAGCGA TCTGCAGGAT CTGGGCATTA CGCTATCTCC GGCGGACCAC GCCGCGAAGC TGGCGGCGCT GACCCAGCGC TGCGGGCTGG ATGGCGTTGT CTGTTCGGCG CAGGAGGCGG TTCGCTTTAA ACAAGAGCTG GGCCAGGCGT TCAAGCTGGT GACGCCTGGC ATTCGTCCGC AAGGTAGCGA CGCGGGTGAC CAGCGGCGCA TTATGACGCC GGAACAGGCG CAGGCGGCGG GGGTAGATTA CATGGTGATT GGTCGTCCGG TAACGCAGTC TGCCGATCCG GCCGCCACCC TGCGCGGTAT CAACGCATCG TTGAATAAGG GGGTATGA
Details for KlaeA.01229.a.B1.GE44531
| HARVESTED ON: | 11/2/2021 |
| SEQUENCED ON: | 11/10/2021 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT STATSSPRVI TSSPVVVALD YDNRDKALAF VERIDPRDCR LKVGKEMFTL LGPQFVRDLH QRGFEVFLDL KFHDIPNTTA RAVAAAAELG VWMVNVHASG GARMMTAARE ALLPFGKEAP LLIAVTVLTS MEASDLQDLG IMLSPADHAA KLAALTKRCG LDGVVCSAQE AVRFKQELGQ EFKLVTPGIR PTGSDAGDQR RIMTPEQAQQ AGVDYMVIGR PVTQSADPVA TLASINASLN KGV
Validated NT Sequence
atcttactca cttatacccc cttattcaac gatgcgttga tactggcaag cgttgccacc ggatcggcgg actgtgtaac cgggcggcca atgaccatat aatcaacccc cgcctgctgc gcctgttccg gggtcatgat tcggcgctga tcgcctgcat cgctacctgt ggggcgaatc cctggcgtga ctaacttaaa ttcctgccct aactcctgct taaagcggac ggcttcctgc gcggaacaga ctacgccgtc cagaccacag cgtttggtta gcgcagccag tttagcggca tgatcggcag gagaaagcat gatgccaagg tcctgcaaat cgctggcttc catgctggtc aatacggtga ccgcaatcag taacggcgcc tcttttccga acggcaacag cgcctcgcgg gccgccgtca tcatgcgcgc accgcctgag gcgtgaacgt tcaccatcca tacgccgagt tccgccgcag cggcgaccgc acgtgcggtg gtattaggaa tatcgtggaa tttcaggtcg aggaacactt caaagccgcg ttgatgcagg tcgcgaacaa actgcggccc cagcagcgtg aacatctcct tacctacttt caaccggcag tcgcggggat cgattcgttc aacaaaggct aacgccttgt cacggttatc ataatcaagc gcaacaacga cgggagagga tgtgataaca cgaggagaag atgttgcagt agacgtcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT STATSSSRVV TSSPIVVALD YDNRDKALAF VDRIDPRDCR LKVGKEMFTL LGPQFVRDLH QRGFEIFLDL KFHDIPNTTA RAVAAAAELG VWMVNVHASG GARMMTAARE ALLPFGKDAP LLIAVTVLTS MEASDLQDLG ITLSPADHAA KLAALTQRCG LDGVVCSAQE AVRFKQELGQ AFKLVTPGIR PQGSDAGDQR RIMTPEQAQA AGVDYMVIGR PVTQSADPAA TLRGINASLN KGV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt ctactgcaac atcttcttcc cgcgttgtca catcctctcc catcgtcgtt gctctcgatt atgataaccg tgataaagcg ctggcgtttg tcgaccgtat cgacccgcgc gactgccggc tcaaggttgg taaagagatg tttaccctgc tggggccgca gtttgttcgc gacctgcacc agcgcggttt tgagattttc ctcgatctaa agtttcacga tatcccgaat accacggcgc gtgcggtcgc cgcagcggca gagcttggcg tctggatggt taacgttcat gcctccggcg gcgcgcgtat gatgaccgcc gcacgtgagg ccctgctgcc tttcggaaaa gatgcgccgc tattaattgc ggtaacggtg ttaaccagca tggaagccag cgatctgcag gatctgggca ttacgctatc tccggcggac cacgccgcga agctggcggc gctgacccag cgctgcgggc tggatggcgt tgtctgttcg gcgcaggagg cggttcgctt taaacaagag ctgggccagg cgttcaagct ggtgacgcct ggcattcgtc cgcaaggtag cgacgcgggt gaccagcggc gcattatgac gccggaacag gcgcaggcgg cgggggtaga ttacatggtg attggtcgtc cggtaacgca gtctgccgat ccggccgcca ccctgcgcgg tatcaacgca tcgttgaata agggggtatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat