KlaeA.00906.a
Cystathionine beta lyase
| CENTER ID: | KlaeA.00906.a |
| ORGANISM: | Klebsiella aerogenes KCTC 2190 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlaeA.00906.a.B1.PW39169 | Full length(KlaeA.00906.a) | 1 | 395 |
Structures
8SA7
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SA8
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SA9
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SAA
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SAB
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SAC
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SAD
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8SAE
DEPOSITED: 3/31/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8U98
DEPOSITED: 9/18/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
8U99
DEPOSITED: 9/18/2023
DETERMINATION: XRay
CLONE: KlaeA.00906.a.B1.GE44851
PROTEIN: KlaeA.00906.a.B1.PW39169
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADKHLDTAL VHAGRSKKYT LGSVNSVIQR ASSLVFDTVE AKKHATRNRA KGELFYGRRG TLTHFSLQEA MCELEGGAGC ALFPCGAAAV ANTILAFVEQ GDHILVTNTA YEPTQDFCTK ILAKLGVTIG WFDPLIGGDI ARLVQPNTRV VFLESPGSIT MEVHDVPAIV AAVRRVAPEA IIMIDNTWAA GVLFKALEFG IDISIQAGTK YLIGHSDAMV GTAVSNERCW AQLRENAYLM GQMVDADTAY MTSRGLRTLA VRLRQHHESS LQIAEWLAQH PQVARVNHPA LPGSKGHEYW KRDFTGSSGL FSFVLNKRLT DAELSAYLDH FSLFSMAYSW GGFESLILAN QPEHIAAIRP DAEVDFSGTL IRLHIGLENV TDLLDDLAAG FARIV
NT Sequence
ATGGCTGATA AACATCTTGA TACTGCGCTG GTTCACGCCG GGCGCAGCAA AAAATACACC CTGGGCTCGG TAAACAGCGT CATTCAGCGC GCCTCATCGC TGGTTTTCGA CACCGTCGAA GCGAAAAAGC ACGCGACGCG CAACCGCGCC AAAGGCGAGC TGTTCTACGG GCGTCGCGGG ACGCTGACCC ATTTCTCCCT GCAGGAAGCA ATGTGCGAGC TGGAAGGCGG CGCCGGCTGC GCCCTCTTCC CCTGCGGCGC GGCGGCGGTC GCGAATACCA TTCTGGCGTT CGTCGAGCAG GGCGACCATA TTCTGGTGAC CAACACCGCC TATGAGCCTA CCCAGGATTT CTGTACGAAA ATTCTCGCCA AACTCGGCGT CACCATCGGC TGGTTCGACC CGCTCATCGG CGGCGATATC GCCCGTCTGG TGCAGCCGAA CACCCGCGTG GTGTTCCTCG AATCCCCCGG CTCCATCACC ATGGAAGTCC ATGATGTTCC GGCGATCGTC GCGGCGGTAC GTCGCGTCGC TCCCGAAGCC ATCATTATGA TCGACAACAC CTGGGCGGCG GGCGTGCTGT TTAAAGCGCT GGAGTTTGGT ATCGATATCT CCATTCAGGC CGGCACCAAA TATCTGATCG GCCATTCCGA CGCGATGGTG GGTACCGCCG TGTCCAATGA GCGCTGCTGG GCGCAGCTGC GCGAAAACGC CTATCTGATG GGGCAAATGG TGGATGCCGA TACCGCCTAT ATGACCAGCC GCGGCCTGCG CACCTTAGCG GTACGCCTGC GTCAGCACCA TGAAAGCAGC CTGCAGATTG CCGAATGGCT GGCGCAGCAT CCACAGGTGG CGCGCGTGAA TCATCCCGCC CTGCCCGGCA GCAAAGGCCA TGAGTACTGG AAGCGCGACT TCACCGGCAG CAGCGGTCTG TTCTCTTTCG TGCTCAATAA ACGCCTGACC GACGCCGAAC TCTCCGCTTA TCTGGACCAC TTCAGCTTGT TCAGCATGGC CTACTCCTGG GGCGGCTTTG AATCGCTGAT TTTAGCCAAC CAGCCGGAAC ACATTGCCGC AATCCGTCCT GATGCTGAAG TTGATTTCAG CGGCACGCTG ATCCGCCTTC ATATCGGGTT AGAAAATGTT ACCGATCTGC TGGATGATTT AGCGGCGGGC TTCGCGCGTA TCGTGTAA
Details for KlaeA.00906.a.B1.PW39169
| PURIFICATION DATe: | 12/14/2022 |
| CONCENTRATION: | 41.12mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 51 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 92 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DKHLDTALVN AGRRKKYTQG SVNSVIQRAS SLVFDTVEAK KHATRNRAKG ELFYGRRGTL THFSLQEAMC ELEGGAGCAL FPCGAAAVAN TILAFVEQGD HVLMTNTAYE PSQDFCTKIL AKLGVTTGWF DPLIGADIAN LIQPNTKVVF LESPGSITME VHDVPAIVAA VRRVAPEAII MIDNTWAAGV LFKALDFGID ISIQAATKYL IGHSDGMIGT AVANARCWEQ LCENAYLMGQ MIDADTAYMT SRGLRTLGVR LRQHHESSLR VAEWLAQHPQ VARVNHPALP GSKGHEFWKR DFSGSSGLFS FVLNKRLTDA ELAAYLDNFS LFSMAYSWGG FESLILANQP EHIAAIRPEA EVDFSGTLIR LHIGLENVDD LLADLAAGFA RIV
Validated NT Sequence
atggctcacc accaccacca ccatatggct gataaacatc ttgataccgc gctggtcaac gccgggcgcc gcaaaaaata tactcagggt tcggtgaata gcgtcatcca gcgcgcctca tcgctggtat tcgataccgt cgaggctaaa aaacacgcca cccgtaaccg cgccaaaggc gagctgtttt atggccgccg cggcacgctg acccactttt cgctgcagga agccatgtgc gaactggaag gcggcgccgg ctgcgccctc ttcccctgcg gcgcggccgc ggtcgctaac acgattctgg ccttcgtcga acagggcgac cacgtgttaa tgaccaacac cgcctatgaa cccagccagg acttctgcac taagatcctc gcaaagctcg gggtgaccac cggctggttc gatccgctca tcggcgcgga tatcgccaat ctgattcaac ccaataccaa agtggtgttc ctcgaatccc ccggctcgat caccatggaa gtgcatgatg tgccggccat cgtcgccgcc gtgcgccgcg tcgcgccgga agctattatc atgatcgata acacctgggc ggcaggggtg ctgtttaaag cgctcgattt tggtatcgat atctctatcc aggcggcaac gaaatattta attggccact ccgacggaat gatcggcacc gcagtggcca acgcccgctg ttgggagcaa ctgtgtgaaa acgcctatct aatgggccag atgatcgatg ccgacaccgc ctatatgacc agccgcggtt tacgcacgct tggcgtccgc ctgcgccaac atcacgaaag cagtctgcgc gtcgccgaat ggctggcgca gcacccacag gtggcgcggg tcaatcatcc ggcgctgccg ggcagcaagg gccacgaatt ctggaagcgc gacttcagcg gcagcagcgg cctgttctct ttcgttctca ataagcgcct caccgacgcc gagctggcag cctatctgga caacttcagt ctgttcagca tggcctactc atggggcggg ttcgaatcgc tgatcctcgc caaccagccg gaacatatcg cggccatccg ccccgaggcg gaagtggatt tcagcggtac ccttattcga ctgcacatag gtttagaaaa tgttgacgat ctgctggcgg atttagcggc aggcttcgcg cgtatcgtgt aa
Expressed Protein Sequence
MAHHHHHHMA DKHLDTALVH AGRSKKYTLG SVNSVIQRAS SLVFDTVEAK KHATRNRAKG ELFYGRRGTL THFSLQEAMC ELEGGAGCAL FPCGAAAVAN TILAFVEQGD HILVTNTAYE PTQDFCTKIL AKLGVTIGWF DPLIGGDIAR LVQPNTRVVF LESPGSITME VHDVPAIVAA VRRVAPEAII MIDNTWAAGV LFKALEFGID ISIQAGTKYL IGHSDAMVGT AVSNERCWAQ LRENAYLMGQ MVDADTAYMT SRGLRTLAVR LRQHHESSLQ IAEWLAQHPQ VARVNHPALP GSKGHEYWKR DFTGSSGLFS FVLNKRLTDA ELSAYLDHFS LFSMAYSWGG FESLILANQP EHIAAIRPDA EVDFSGTLIR LHIGLENVTD LLDDLAAGFA RIV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG ATAAACATCT TGATACTGCG CTGGTTCACG CCGGGCGCAG CAAAAAATAC ACCCTGGGCT CGGTAAACAG CGTCATTCAG CGCGCCTCAT CGCTGGTTTT CGACACCGTC GAAGCGAAAA AGCACGCGAC GCGCAACCGC GCCAAAGGCG AGCTGTTCTA CGGGCGTCGC GGGACGCTGA CCCATTTCTC CCTGCAGGAA GCAATGTGCG AGCTGGAAGG CGGCGCCGGC TGCGCCCTCT TCCCCTGCGG CGCGGCGGCG GTCGCGAATA CCATTCTGGC GTTCGTCGAG CAGGGCGACC ATATTCTGGT GACCAACACC GCCTATGAGC CTACCCAGGA TTTCTGTACG AAAATTCTCG CCAAACTCGG CGTCACCATC GGCTGGTTCG ACCCGCTCAT CGGCGGCGAT ATCGCCCGTC TGGTGCAGCC GAACACCCGC GTGGTGTTCC TCGAATCCCC CGGCTCCATC ACCATGGAAG TCCATGATGT TCCGGCGATC GTCGCGGCGG TACGTCGCGT CGCTCCCGAA GCCATCATTA TGATCGACAA CACCTGGGCG GCGGGCGTGC TGTTTAAAGC GCTGGAGTTT GGTATCGATA TCTCCATTCA GGCCGGCACC AAATATCTGA TCGGCCATTC CGACGCGATG GTGGGTACCG CCGTGTCCAA TGAGCGCTGC TGGGCGCAGC TGCGCGAAAA CGCCTATCTG ATGGGGCAAA TGGTGGATGC CGATACCGCC TATATGACCA GCCGCGGCCT GCGCACCTTA GCGGTACGCC TGCGTCAGCA CCATGAAAGC AGCCTGCAGA TTGCCGAATG GCTGGCGCAG CATCCACAGG TGGCGCGCGT GAATCATCCC GCCCTGCCCG GCAGCAAAGG CCATGAGTAC TGGAAGCGCG ACTTCACCGG CAGCAGCGGT CTGTTCTCTT TCGTGCTCAA TAAACGCCTG ACCGACGCCG AACTCTCCGC TTATCTGGAC CACTTCAGCT TGTTCAGCAT GGCCTACTCC TGGGGCGGCT TTGAATCGCT GATTTTAGCC AACCAGCCGG AACACATTGC CGCAATCCGT CCTGATGCTG AAGTTGATTT CAGCGGCACG CTGATCCGCC TTCATATCGG GTTAGAAAAT GTTACCGATC TGCTGGATGA TTTAGCGGCG GGCTTCGCGC GTATCGTGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT