SSGCID: Target Page

KlaeA.00825.a

Asparagine--tRNA ligase (EC 6.1.1.22) (Asparaginyl-tRNA synthetase) (AsnRS)

CENTER ID:	KlaeA.00825.a
ORGANISM:	Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlaeA.00825.a.UX1.PW39323	full length		1	466
KlaeA.00825.a.UX1.PW39313	full length		1	466

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1028307.3.peg.3047
UniProt:	A0A0H3FQR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVVPVADVL QGRVAVDSEV TVRGWVRTRR DSKAGFSFLA VYDGSCFDPV QAVINNSLPN YNQEVLRLTT GCSVIVTGKV VASQGQGQSF EIQASNVEVT GWVEDPDTYP MAAKRHSIEY LREVAHLRPR TNLIGAVARV RHTLAQALHR FFDEQGFFWV STPLITASDT EGAGEMFRVS TLDLENLPRN DQGKVDFDKD FFGKESFLTV SGQLNGETYA CALSKIYTFG PTFRAENSNT SRHLAEFWML EPEVAFASLN DVAGLAEAML KYVFKAVLEE RADDMKFFAE RVDKDAIDRL QRFVSADFAQ VDYTDAVTIL ENCGKQFENP VYWGVDLSSE HERYLAEEHF KAPVVVKNYP KDIKAFYMRL NEDGKTVAAM DVLAPGIGEI IGGSQREERL DVLDARMAEM GLNKEDYWWY RDLRRYGTVP HSGFGLGFER LIAYVTGVQN VRDVIPFPRT PRNATF

NT Sequence

atgagcgttg tgcctgtagc cgacgtactc cagggccgcg ttgccgttga cagcgaagtc accgtgcgcg gatgggtgcg tacccgccga gattctaaag ctggcttttc tttcctcgcc gtctatgacg gttcctgctt tgatcctgta caggccgtta ttaataattc tctgcccaat tacaatcagg aagtgttgcg cctgaccacc ggctgctcgg ttatcgtcac cggtaaagtc gtggcctccc agggtcaggg acagagcttt gaaatccagg cctccaatgt ggaagtcacc ggctgggttg aggatccgga cacctacccg atggcggcga agcgccacag catcgaatat ctgcgtgaag tggcgcacct gcgtccgcgc accaacctga ttggcgcggt agcgcgcgta cgtcacaccc tggcgcaggc gctgcaccgt ttcttcgacg aacagggttt cttctgggta tccaccccgc tgatcaccgc ttccgatacc gaaggcgccg gtgaaatgtt ccgcgtatca acgctggatc tggaaaacct gccgcgtaac gaccagggta aagtcgactt cgataaagac ttcttcggta aagagtcgtt cctgaccgtt tccggtcagt tgaacggtga gacctatgcc tgcgcattgt ccaaaatcta taccttcggc ccaaccttcc gcgccgaaaa ctccaacacc agccgccacc tggcggagtt ctggatgctg gagccagagg tagccttcgc cagcctcaac gatgttgccg gtctggcgga agccatgctg aagtacgtct tcaaggcggt gctggaagag cgtgcggatg acatgaagtt cttcgccgag cgcgtagata aagacgccat cgaccgtctg cagcgttttg tctctgctga tttcgcccag gttgattaca ccgacgccgt cactatcctg gaaaactgcg gcaagcagtt tgagaacccg gtgtactggg gcgtggatct ctcctccgag catgagcgtt atctggccga agagcacttc aaagcgccgg tggtcgtgaa aaactatccg aaagacatta aggcgttcta catgcgcctt aacgaagacg gtaagaccgt cgccgcaatg gacgttctgg cgccgggcat cggcgaaatc atcggcgggt cccagcgtga agagcgtctc gacgtgctgg acgcgcgtat ggccgaaatg ggtctgaata aagaagatta ctggtggtat cgcgacctgc gtcgttacgg caccgtaccg cattctggct tcggtctggg cttcgagcgt cttatcgcct acgtaaccgg cgtacagaac gttcgcgacg tgattccttt cccacgtaca ccacgtaacg ccacattcta a

Details for KlaeA.00825.a.UX1.PW39323

PURIFICATION DATe:	10/3/2024
CONCENTRATION:	14.8mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 30 mM Imidazole, 0.025% Azide, 1 mM TCEP *3C cleavage was performed on original protein stock to remove His-tag; only reverse IMAC was performed, no SEC since it was performed in the original purification.
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSVVPVADV LQGRVAVDSE VTVRGWVRTR RDSKAGFSFL AVYDGSCFDP VQAVINNSLP NYNQEVLRLT TGCSVIVTGK VVASQGQGQS FEIQASNVEV TGWVEDPDTY PMAAKRHSIE YLREVAHLRP RTNLIGAVAR VRHTLAQALH RFFDEQGFFW VSTPLITASD TEGAGEMFRV STLDLENLPR NDQGKVDFDK DFFGKESFLT VSGQLNGETY ACALSKIYTF GPTFRAENSN TSRHLAEFWM LEPEVAFASL NDVAGLAEAM LKYVFKAVLE ERADDMKFFA ERVDKDAIDR LQRFVSADFA QVDYTDAVTI LENCGKQFEN PVYWGVDLSS EHERYLAEEH FKAPVVVKNY PKDIKAFYMR LNEDGKTVAA MDVLAPGIGE IIGGSQREER LDVLDARMAE MGLNKEDYWW YRDLRRYGTV PHSGFGLGFE RLIAYVTGVQ NVRDVIPFPR TPRNATF

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA ATTAATTCTT AGAAAAACTC ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGTTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TAGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCTCTAG AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACCATGG CTCATCACCA TCACCATCAT ATGGGTACCC TGGAAGCTCA GACCCAGGGT CCTGGTTCGA TGTCCGTAGT ACCCGTTGCG GATGTTCTGC AAGGACGTGT CGCAGTGGAT TCTGAGGTGA CAGTCAGAGG TTGGGTTCGA ACGCGTCGTG ACAGCAAGGC CGGTTTCTCA TTTCTGGCGG TGTACGATGG AAGTTGTTTC GACCCAGTTC AAGCTGTGAT CAACAACTCC TTGCCTAACT ATAACCAAGA GGTTCTCAGA TTAACTACGG GTTGTTCTGT CATAGTGACT GGGAAGGTTG TAGCGTCACA AGGACAAGGG CAATCATTCG AGATTCAAGC TAGCAACGTA GAGGTTACTG GATGGGTCGA AGACCCTGAT ACATATCCAA TGGCAGCCAA ACGTCATTCA ATTGAGTACC TTCGAGAGGT AGCACATTTA AGACCAAGAA CGAATCTCAT CGGTGCTGTG GCTAGAGTCC GACATACTTT AGCCCAAGCA CTCCATCGCT TCTTCGATGA GCAAGGGTTC TTTTGGGTGA GCACTCCCCT CATTACGGCG TCAGACACTG AGGGTGCAGG GGAGATGTTT CGTGTGAGTA CTTTAGACTT GGAGAATCTT CCCCGAAATG ATCAAGGGAA GGTGGATTTT GACAAGGATT TCTTTGGGAA GGAAAGCTTT CTCACTGTCT CGGGACAACT TAATGGGGAA ACATACGCAT GTGCCCTGTC TAAGATTTAC ACGTTTGGTC CCACGTTTCG TGCTGAGAAT AGTAATACAA GTCGACATTT AGCAGAATTT TGGATGCTTG AACCCGAAGT TGCGTTTGCA TCTCTGAATG ACGTAGCTGG ATTAGCTGAG GCTATGTTAA AATATGTATT TAAAGCCGTT CTTGAGGAAC GGGCAGACGA TATGAAATTC TTTGCAGAAA GAGTTGACAA GGATGCTATT GATCGCTTAC AACGCTTCGT GTCAGCAGAC TTTGCGCAAG TAGACTATAC TGATGCGGTT ACAATTCTTG AGAATTGTGG AAAACAATTC GAAAATCCTG TATATTGGGG TGTTGACCTG AGTTCAGAAC ACGAACGCTA CTTAGCAGAG GAACATTTTA AGGCCCCTGT TGTTGTCAAG AATTACCCTA AGGATATAAA AGCTTTCTAT ATGCGTCTGA ATGAGGATGG CAAAACAGTT GCAGCTATGG ATGTCTTGGC TCCCGGAATT GGAGAGATTA TTGGTGGCTC ACAACGCGAG GAACGCCTTG ATGTATTAGA TGCACGCATG GCGGAGATGG GCTTGAACAA GGAAGACTAT TGGTGGTACC GGGATCTTCG CCGGTATGGG ACTGTCCCCC ACAGCGGATT TGGCTTGGGA TTTGAACGGC TCATTGCTTA TGTGACGGGA GTTCAAAATG TAAGAGATGT TATACCCTTT CCGCGCACCC CGCGGAATGC GACCTTTTGA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GAT

Details for KlaeA.00825.a.UX1.PW39313

PURIFICATION DATe:	7/30/2024
CONCENTRATION:	11.4mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	42
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)