KlaeA.00771.a

Diaminopimelate decarboxylase (LysA)

CENTER ID: KlaeA.00771.a
ORGANISM: Klebsiella aerogenes KCTC 2190
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlaeA.00771.a.B1.PW39176 Full length(KlaeA.00771.a) 1 420

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRSLNATDT DLTADNLLRL PAEFGCPVWV YDAQIIRRQI AQLSQFDVVR FAQKACSNIH ILRLMREQGV KVDSVSLGEI ERALAAGYDP VQHPDDIVFT ADLIDRATLE RVKALQIPVN AGSVDMLSQL GEVSPGHRVW LRVNPGFGHG HSQKTNTGGE NSKHGIWFSQ LPQALEVIRR HSLQLVGIHM HIGSGVDYGH LEQVCGAMVR QVVEFGQDLQ AISAGGGLSI PYHEGEEAID TDHYFGLWNA AREQIAKHLG HPVKLEIEPG RFLVAESGVL VSQVRSVKEM GSRHFVLIDA GFNDLMRPAM YGSYHHITAL AADGRDLSGE PQLETVVAGP LCESGDVFTQ QEGGKVETRL LPAVAPGDYL VLHDTGAYGA SMSSNYNSRP LLPEVLFDNG SARLIRRRQT IEELLALELI
NT Sequence
ATGCCACGCT CGCTGAACGC CACCGATACC GATCTGACCG CCGACAATCT ACTGCGCCTG CCCGCTGAGT TCGGCTGCCC GGTATGGGTC TATGACGCGC AGATTATCCG CCGCCAGATT GCCCAACTTA GCCAGTTTGA CGTGGTGCGT TTTGCGCAGA AGGCCTGCTC CAATATTCAT ATTCTGCGTC TGATGCGCGA GCAGGGCGTG AAGGTGGATT CGGTGTCGCT TGGCGAGATC GAACGCGCGC TGGCGGCAGG TTACGATCCC GTGCAGCATC CGGACGATAT CGTCTTTACC GCCGATCTGA TCGATCGTGC GACCCTTGAG CGGGTGAAAG CATTACAGAT CCCAGTGAAT GCCGGATCCG TGGATATGCT TAGCCAACTG GGCGAAGTCT CGCCGGGTCA CCGCGTCTGG CTACGCGTCA ACCCGGGATT TGGTCATGGC CACAGCCAGA AGACCAACAC CGGCGGCGAA AACAGCAAAC ACGGTATCTG GTTCAGCCAG CTGCCGCAGG CGCTGGAGGT GATTCGCCGC CATTCGCTGC AGCTGGTCGG CATCCATATG CATATCGGCT CCGGCGTTGA TTACGGTCAT CTTGAGCAAG TATGCGGCGC GATGGTGCGC CAGGTGGTGG AGTTTGGTCA GGATCTGCAG GCGATCTCCG CCGGCGGCGG TTTATCGATC CCGTATCATG AAGGCGAAGA GGCGATCGAT ACCGATCACT ACTTTGGCCT GTGGAATGCG GCGCGCGAGC AGATCGCCAA ACATCTGGGG CATCCGGTGA AGCTGGAGAT TGAACCGGGG CGTTTCCTGG TGGCGGAGTC CGGCGTGCTG GTATCGCAGG TGCGTAGCGT GAAAGAGATG GGCAGCCGCC ACTTCGTGCT GATCGACGCC GGCTTTAACG ATCTGATGCG CCCGGCCATG TACGGCAGCT ATCATCATAT TACCGCGCTG GCGGCGGACG GGCGCGACCT GAGCGGCGAA CCGCAGCTGG AAACCGTGGT CGCGGGGCCG CTGTGCGAAT CCGGCGACGT CTTTACCCAA CAGGAGGGCG GTAAAGTGGA AACTCGCCTG CTGCCGGCGG TGGCGCCGGG CGATTACCTG GTGCTGCACG ACACCGGCGC CTACGGCGCA TCGATGTCTT CAAACTACAA CAGCCGCCCG CTACTACCGG AAGTGCTGTT TGATAACGGC AGCGCGCGCC TGATTCGCCG TCGCCAGACC ATTGAAGAAC TGCTGGCGCT GGAGCTTATT TAA
Details for KlaeA.00771.a.B1.PW39176
PURIFICATION DATe: 1/4/2023
CONCENTRATION: 29.07mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 63
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP RSLNATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPVQ HPDDIVFTAD LIDRATLERV KALQIPVNAG SVDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWFSQLP QALEVIRRHS LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VEFGQDLQAI SAGGGLSIPY HEGEEAIDTD HYFGLWNAAR EQIAKHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAA DGRDLSGEPQ LETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDNGSA RLIRRRQTIE ELLALELI
Validated NT Sequence
atggctcacc accaccacca ccatatgcca cgctcgctga acgccaccga taccgatctg accgccgaca atctactgcg cctgcccgct gagttcggct gcccggtatg ggtctatgac gcgcagatta tccgccgcca gattgcccaa cttagccagt ttgacgtggt gcgttttgcg cagaaggcct gctccaatat tcatattctg cgtctgatgc gcgagcaggg cgtgaaggtg gattcggtgt cgcttggcga gatcgaacgc gcgctggcgg caggttacga tcccgtgcag catccggacg atatcgtctt taccgccgat ctgatcgatc gtgcgaccct tgagcgggtg aaagcattac agatcccagt gaatgccgga tccgtggata tgcttagcca actgggcgaa gtctcgccgg gtcaccgcgt ctggctacgc gtcaacccgg gatttggtca tggccacagc cagaagacca acaccggcgg cgaaaacagc aaacacggta tctggttcag ccagctgccg caggcgctgg aggtgattcg ccgccattcg ctgcagctgg tcggcatcca tatgcatatc ggctccggcg ttgattacgg tcatcttgag caagtatgcg gcgcgatggt gcgccaggtg gtggagtttg gtcaggatct gcaggcgatc tccgccggcg gcggtttatc gatcccgtat cacgaaggcg aagaggcgat cgataccgat cactactttg gcctgtggaa tgcggcgcgc gagcagatcg ccaaacatct ggggcatccg gtgaagctgg agattgaacc ggggcgtttc ctggtggcgg agtccggcgt gctggtatcg caggtgcgta gcgtgaaaga gatgggcagc cgccacttcg tgctgatcga cgccggcttt aacgatctga tgcgcccggc catgtacggc agctatcatc atattaccgc gctggcggcg gacgggcgcg acctgagcgg cgaaccgcag ctggaaaccg tggtcgcggg gccgctgtgc gaatccggcg acgtctttac ccaacaggag ggcggtaaag tggaaactcg cctgctgccg gcggtggcgc cgggcgatta cctggtgctg cacgacaccg gcgcctacgg cgcatcgatg tcttcaaact acaacagccg cccgctacta ccggaagtgc tgtttgataa cggcagcgcg cgcctgattc gccgtcgcca gaccattgaa gaactgctgg cgctggagct tatttaa
Expressed Protein Sequence
MAHHHHHHMP RSLNATDTDL TADNLLRLPA EFGCPVWVYD AQIIRRQIAQ LSQFDVVRFA QKACSNIHIL RLMREQGVKV DSVSLGEIER ALAAGYDPVQ HPDDIVFTAD LIDRATLERV KALQIPVNAG SVDMLSQLGE VSPGHRVWLR VNPGFGHGHS QKTNTGGENS KHGIWFSQLP QALEVIRRHS LQLVGIHMHI GSGVDYGHLE QVCGAMVRQV VEFGQDLQAI SAGGGLSIPY HEGEEAIDTD HYFGLWNAAR EQIAKHLGHP VKLEIEPGRF LVAESGVLVS QVRSVKEMGS RHFVLIDAGF NDLMRPAMYG SYHHITALAA DGRDLSGEPQ LETVVAGPLC ESGDVFTQQE GGKVETRLLP AVAPGDYLVL HDTGAYGASM SSNYNSRPLL PEVLFDNGSA RLIRRRQTIE ELLALELI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAC GCTCGCTGAA CGCCACCGAT ACCGATCTGA CCGCCGACAA TCTACTGCGC CTGCCCGCTG AGTTCGGCTG CCCGGTATGG GTCTATGACG CGCAGATTAT CCGCCGCCAG ATTGCCCAAC TTAGCCAGTT TGACGTGGTG CGTTTTGCGC AGAAGGCCTG CTCCAATATT CATATTCTGC GTCTGATGCG CGAGCAGGGC GTGAAGGTGG ATTCGGTGTC GCTTGGCGAG ATCGAACGCG CGCTGGCGGC AGGTTACGAT CCCGTGCAGC ATCCGGACGA TATCGTCTTT ACCGCCGATC TGATCGATCG TGCGACCCTT GAGCGGGTGA AAGCATTACA GATCCCAGTG AATGCCGGAT CCGTGGATAT GCTTAGCCAA CTGGGCGAAG TCTCGCCGGG TCACCGCGTC TGGCTACGCG TCAACCCGGG ATTTGGTCAT GGCCACAGCC AGAAGACCAA CACCGGCGGC GAAAACAGCA AACACGGTAT CTGGTTCAGC CAGCTGCCGC AGGCGCTGGA GGTGATTCGC CGCCATTCGC TGCAGCTGGT CGGCATCCAT ATGCATATCG GCTCCGGCGT TGATTACGGT CATCTTGAGC AAGTATGCGG CGCGATGGTG CGCCAGGTGG TGGAGTTTGG TCAGGATCTG CAGGCGATCT CCGCCGGCGG CGGTTTATCG ATCCCGTATC ATGAAGGCGA AGAGGCGATC GATACCGATC ACTACTTTGG CCTGTGGAAT GCGGCGCGCG AGCAGATCGC CAAACATCTG GGGCATCCGG TGAAGCTGGA GATTGAACCG GGGCGTTTCC TGGTGGCGGA GTCCGGCGTG CTGGTATCGC AGGTGCGTAG CGTGAAAGAG ATGGGCAGCC GCCACTTCGT GCTGATCGAC GCCGGCTTTA ACGATCTGAT GCGCCCGGCC ATGTACGGCA GCTATCATCA TATTACCGCG CTGGCGGCGG ACGGGCGCGA CCTGAGCGGC GAACCGCAGC TGGAAACCGT GGTCGCGGGG CCGCTGTGCG AATCCGGCGA CGTCTTTACC CAACAGGAGG GCGGTAAAGT GGAAACTCGC CTGCTGCCGG CGGTGGCGCC GGGCGATTAC CTGGTGCTGC ACGACACCGG CGCCTACGGC GCATCGATGT CTTCAAACTA CAACAGCCGC CCGCTACTAC CGGAAGTGCT GTTTGATAAC GGCAGCGCGC GCCTGATTCG CCGTCGCCAG ACCATTGAAG AACTGCTGGC GCTGGAGCTT ATTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT