KlaeA.00150.a
D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)
| CENTER ID: | KlaeA.00150.a |
| ORGANISM: | Klebsiella aerogenes KCTC 2190 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| KlaeA.00150.a.B1.PW39177 | Full length(KlaeA.00150.a) | 1 | 456 |
Structures
9MH4
DEPOSITED: 12/11/2024
DETERMINATION: XRay
CLONE: KlaeA.00150.a.B1.GE44839
PROTEIN: KlaeA.00150.a.B1.PW39177
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
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AA Sequence
MSNSAMSVVI LAAGKGTRMY SDLPKVLHTL AGKPMVQHVI DAAKDLGAAA VHLVYGHGGD LLRQTLHEDN LNWVLQAEQL GTGHAMQQAA PFFNDDEDIL MLYGDVPLIS VETLQRLRAA KPQGGIGLLT VKLDDPTGYG RITRENGQVT GIVEHKDASE AQRQIQEINT GILVANGADL KRWLAKLTNN NAQGEYYITD IIAMAHQEGH EIVAVHPQRL SEVEGVNNRL QLARLERVYQ SEQAEKLLLA GVMLRDPARF DLRGVLKHGR DVEIDTNVIL QGHVVLGDRV KIGAGCVIKD SVIGDDCEIS PYSVVEDAQL QAACTIGPFA RLRPGAELLE GAHVGNFVEM KKARLGKGSK AGHLSYLGDA EIGDNVNIGA GTITCNYDGA NKHKTIIGDD VFVGSDTQLV APVSVGNGVT IAAGTTVTRN IADNELVLSR VPQVHKQGWQ RPVKKK
NT Sequence
ATGTCAAACA GTGCGATGAG CGTGGTTATC CTTGCCGCAG GCAAAGGGAC CCGCATGTAT TCCGATCTTC CTAAGGTGCT GCATACTCTG GCCGGGAAGC CAATGGTGCA ACACGTCATT GATGCGGCTA AAGATTTAGG CGCTGCCGCG GTACACCTGG TGTACGGCCA CGGCGGCGAT CTGCTGCGTC AAACGTTGCA TGAAGATAAC CTCAACTGGG TGCTGCAAGC AGAGCAGCTC GGTACCGGCC ATGCGATGCA GCAGGCGGCG CCGTTCTTCA ATGATGATGA AGATATCTTG ATGCTCTATG GCGATGTGCC GCTGATTTCG GTTGAAACCC TACAGCGCCT GCGCGCCGCA AAACCGCAGG GCGGTATTGG CCTGTTGACG GTGAAGCTGG ATGATCCAAC CGGTTATGGG CGTATCACGC GTGAAAATGG CCAGGTGACA GGTATCGTTG AGCATAAAGA CGCCAGCGAA GCTCAGCGCC AGATTCAGGA AATCAATACC GGGATTCTGG TTGCTAACGG CGCGGACCTG AAACGCTGGC TGGCGAAGCT CACCAACAAT AACGCCCAGG GCGAATACTA CATCACCGAT ATTATCGCGA TGGCCCATCA GGAAGGTCAT GAGATCGTTG CGGTGCATCC TCAGCGCCTG AGCGAAGTTG AAGGGGTGAA CAATCGCCTG CAACTTGCGC GTCTGGAAAG AGTGTATCAG TCAGAGCAGG CGGAAAAACT GCTGTTGGCG GGCGTGATGC TGCGCGATCC GGCGCGTTTC GATCTGCGCG GCGTGCTGAA ACACGGGCGC GATGTCGAAA TTGATACTAA CGTTATTCTG CAAGGTCATG TCGTGCTGGG CGATCGCGTG AAGATCGGCG CCGGCTGCGT CATCAAAGAC AGCGTCATCG GTGATGACTG CGAAATCAGC CCGTATAGCG TGGTTGAAGA TGCGCAGCTG CAGGCCGCCT GCACCATCGG CCCGTTTGCG CGTTTGCGTC CGGGGGCTGA GCTGCTGGAA GGCGCTCACG TCGGCAACTT TGTCGAAATG AAGAAAGCGC GTCTGGGCAA AGGTTCGAAG GCAGGTCATC TCTCCTACCT CGGCGATGCT GAAATTGGCG ACAACGTTAA TATCGGCGCG GGTACTATCA CCTGTAACTA TGATGGCGCA AATAAGCATA AAACGATCAT CGGCGATGAT GTGTTTGTCG GTTCTGATAC GCAACTGGTC GCGCCGGTCA GCGTCGGTAA TGGCGTGACG ATTGCCGCGG GCACCACCGT CACGCGTAAT ATAGCTGACA ACGAACTGGT GCTAAGTCGC GTGCCGCAGG TCCACAAACA GGGCTGGCAG CGACCGGTGA AGAAAAAGTA G
Details for KlaeA.00150.a.B1.PW39177
| PURIFICATION DATe: | 1/4/2023 |
| CONCENTRATION: | 21.79mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 65 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NSAMSVVILA AGKGTRMYSD LPKVLHTLAG KPMVQHVIDA AKDLGAAAVH LVYGHGGDLL RQTLHEDNLN WVLQAEQLGT GHAMQQAAPF FSDDEDILML YGDVPLISVE TLQRLRAAKP QGGIGLLTVK LDDPTGYGRI TRENGQVTGI VEHKDASEAQ RQIQEINTGI LVANGADLKR WLAKLTNNNA QGEYYITDII AMAYQEGHEI VAVHPQRLSE VEGVNNRLQL ARLERVYQSE QAEKLLLAGV MLRDPARFDL RGVLKHGRDV EIDTNVILQG HVVLGDRVKI GAGCVIKDSV IGDDCEISPY SVVEDAQLQA ACTIGPFARL RPGAELLEGA HVGNFVEMKK ARLGKGSKAG HLSYLGDAEI GDNVNIGAGT ITCNYDGANK HKTIIGDDVF VGSDTQLVAP VSVGNGVTIA AGTTVTRNIA DNELVLSRVP QVHKQGWQRP VKKK
Validated NT Sequence
atggctcacc accaccacca ccatatgtca aacagtgcga tgagcgtggt tatccttgcc gcaggcaaag ggacccgcat gtattccgat cttcctaagg tgctgcatac tctggccggg aagccaatgg ttcagcacgt cattgatgcg gctaaagatt taggcgctgc cgcggtacac ctggtgtacg gtcacggcgg cgatctgctg cgtcaaacgt tgcatgaaga taacctcaac tgggtgctgc aagcagagca gctcggtacc ggccatgcga tgcagcaggc ggcgccgttc tttagtgatg atgaagatat cttgatgctc tatggcgatg tgccgctgat ttcggttgaa acgctacagc gcctgcgcgc cgcaaaaccg cagggcggta tcggcctgtt gacggtgaag ctggatgatc caaccggtta tgggcgcatt acgcgtgaaa atggccaggt gacaggcatc gttgagcata aagacgccag cgaagctcag cgccagattc aggaaatcaa taccgggatt ctggttgcta acggcgcgga cctgaaacgc tggctggcaa agctcactaa caataacgcc cagggcgaat actacatcac cgatattatc gcgatggcct atcaggaagg tcatgagatc gttgcggtgc atcctcagcg cctgagcgaa gttgaagggg tgaacaatcg cctgcaactt gcgcgtctgg aacgagtata tcagtcagag caggcggaaa aactgctgct ggcgggcgtg atgctgcgcg atccggcgcg tttcgatctg cgcggcgtgc tcaaacacgg gcgcgatgtc gaaattgata ctaacgttat tctgcaaggt catgtcgtgc tgggcgatcg cgtgaagatc ggcgccggct gcgtcatcaa agacagcgtc atcggtgatg actgcgaaat cagcccgtat agcgtggttg aagatgcgca gctgcaggcc gcctgcacca tcggcccgtt tgcgcgtctg cgtccggggg ctgagctgct ggaaggcgct cacgtcggca actttgtcga aatgaagaaa gcgcgtctgg gcaaaggttc gaaggcaggt catctctcct acctcggcga tgctgaaatt ggcgacaacg ttaatatcgg cgcgggtact atcacctgta actatgatgg cgcaaataag cataaaacga tcatcggcga tgatgtgttt gtcggttctg atacgcaact ggtcgcgccg gtcagcgtcg gtaatggcgt gacgattgcc gcgggcacca ccgtcacgcg taatatagct gacaacgaac tggtgctaag tcgcgtgccg caggtccaca aacagggctg gcagcgaccg gtgaagaaaa agtaa
Expressed Protein Sequence
MAHHHHHHMS NSAMSVVILA AGKGTRMYSD LPKVLHTLAG KPMVQHVIDA AKDLGAAAVH LVYGHGGDLL RQTLHEDNLN WVLQAEQLGT GHAMQQAAPF FNDDEDILML YGDVPLISVE TLQRLRAAKP QGGIGLLTVK LDDPTGYGRI TRENGQVTGI VEHKDASEAQ RQIQEINTGI LVANGADLKR WLAKLTNNNA QGEYYITDII AMAHQEGHEI VAVHPQRLSE VEGVNNRLQL ARLERVYQSE QAEKLLLAGV MLRDPARFDL RGVLKHGRDV EIDTNVILQG HVVLGDRVKI GAGCVIKDSV IGDDCEISPY SVVEDAQLQA ACTIGPFARL RPGAELLEGA HVGNFVEMKK ARLGKGSKAG HLSYLGDAEI GDNVNIGAGT ITCNYDGANK HKTIIGDDVF VGSDTQLVAP VSVGNGVTIA AGTTVTRNIA DNELVLSRVP QVHKQGWQRP VKKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA ACAGTGCGAT GAGCGTGGTT ATCCTTGCCG CAGGCAAAGG GACCCGCATG TATTCCGATC TTCCTAAGGT GCTGCATACT CTGGCCGGGA AGCCAATGGT GCAACACGTC ATTGATGCGG CTAAAGATTT AGGCGCTGCC GCGGTACACC TGGTGTACGG CCACGGCGGC GATCTGCTGC GTCAAACGTT GCATGAAGAT AACCTCAACT GGGTGCTGCA AGCAGAGCAG CTCGGTACCG GCCATGCGAT GCAGCAGGCG GCGCCGTTCT TCAATGATGA TGAAGATATC TTGATGCTCT ATGGCGATGT GCCGCTGATT TCGGTTGAAA CCCTACAGCG CCTGCGCGCC GCAAAACCGC AGGGCGGTAT TGGCCTGTTG ACGGTGAAGC TGGATGATCC AACCGGTTAT GGGCGTATCA CGCGTGAAAA TGGCCAGGTG ACAGGTATCG TTGAGCATAA AGACGCCAGC GAAGCTCAGC GCCAGATTCA GGAAATCAAT ACCGGGATTC TGGTTGCTAA CGGCGCGGAC CTGAAACGCT GGCTGGCGAA GCTCACCAAC AATAACGCCC AGGGCGAATA CTACATCACC GATATTATCG CGATGGCCCA TCAGGAAGGT CATGAGATCG TTGCGGTGCA TCCTCAGCGC CTGAGCGAAG TTGAAGGGGT GAACAATCGC CTGCAACTTG CGCGTCTGGA AAGAGTGTAT CAGTCAGAGC AGGCGGAAAA ACTGCTGTTG GCGGGCGTGA TGCTGCGCGA TCCGGCGCGT TTCGATCTGC GCGGCGTGCT GAAACACGGG CGCGATGTCG AAATTGATAC TAACGTTATT CTGCAAGGTC ATGTCGTGCT GGGCGATCGC GTGAAGATCG GCGCCGGCTG CGTCATCAAA GACAGCGTCA TCGGTGATGA CTGCGAAATC AGCCCGTATA GCGTGGTTGA AGATGCGCAG CTGCAGGCCG CCTGCACCAT CGGCCCGTTT GCGCGTTTGC GTCCGGGGGC TGAGCTGCTG GAAGGCGCTC ACGTCGGCAA CTTTGTCGAA ATGAAGAAAG CGCGTCTGGG CAAAGGTTCG AAGGCAGGTC ATCTCTCCTA CCTCGGCGAT GCTGAAATTG GCGACAACGT TAATATCGGC GCGGGTACTA TCACCTGTAA CTATGATGGC GCAAATAAGC ATAAAACGAT CATCGGCGAT GATGTGTTTG TCGGTTCTGA TACGCAACTG GTCGCGCCGG TCAGCGTCGG TAATGGCGTG ACGATTGCCG CGGGCACCAC CGTCACGCGT AATATAGCTG ACAACGAACT GGTGCTAAGT CGCGTGCCGC AGGTCCACAA ACAGGGCTGG CAGCGACCGG TGAAGAAAAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT