InvbU.18715.a

Hemagglutinin (HA)

CENTER ID: InvbU.18715.a
ORGANISM: Influenzavirus A Influenza A virus A/Iowa/1943
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
InvbU.18715.a.KN11.PD38347 full length 18 508
External Resources
RESOURCE REFERENCE ID
IRD: link
UniProt: A4GCK8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKANLLVLLC ALAAADADTI CIGYHANNST DTVDTVLEKN VTVTHSVNLL EDSHNGKLCR LKGIAPLQLG KCNIAGWILG NPECESLLSE RSWSYIVETP NSENGTCYPG DFIDYEELRE QLSSVSSFER FEIFSKESSW PKHTTGGVTA ACSHAGKSSF YRNLLWLTEK DGSYPNLNNS YVNKKGKEVL VLWGVHHPSN IKDQQTLYQK ENAYVSVVSS NYNRRFTPEI AERPKVRGQA GRINYYWTLL KPGDTIMFEA NGNLIAPWYA FALSRGFGSG IITSNASMHE CDTKCQTPQG AINSSLPFQN IHPVTIGECP KYVRSTKLRM VTGLRNIPSI QSRGLFGAIA GFIEGGWTGM IDGWYGYHHQ NEQGSGYAAD QKSTQNAING ITNKVNSVIE KMNTQFTAVG KEFNNLEKRM ENLNKKVDDG FLDIWTYNAE LLVLLENERT LDFHDSNVKN LYEKVKNQLR NNAKEIGNGC FEFYHKCNNE CMESVKNGTY DYPKYSEESK LNREKIDGVK LESMGVYQIL AIYSTVASSL VLLVSLGAIS FWMCSNGSLQ CRICI
NT Sequence
atgaaggcaa acctactggt cctgttatgt gcacttgcag ctgcagatgc agacacaata tgtataggct accatgcaaa caactcaacc gacactgttg acacagtact cgaaaagaat gtgacagtga cacactctgt caacctactc gaagacagcc acaacgggaa attatgtaga ttaaaaggaa tagccccact acaattgggg aaatgtaaca ttgccggatg gatcttggga aacccagaat gcgaatcact gctttcagag agatcatggt cctacattgt agaaacacca aactctgaaa atggaacatg ttacccagga gatttcatcg actatgagga actgagggag caattgagct cagtatcatc attcgaaaga ttcgaaatat tctccaagga aagctcatgg cccaaacaca caaccggagg agtaacggca gcatgctccc atgcgggaaa aagtagtttt tacagaaatt tgctatggct gacggagaag gatggctcat acccgaatct gaacaattcc tatgtgaaca agaaagggaa agaagtcctt gtactatggg gtgttcatca cccgtctaac ataaaggatc aacagaccct ctatcagaaa gaaaatgctt atgtctctgt agtgtcttca aactataaca ggagattcac cccggaaata gcagaaagac ccaaagtaag aggtcaagca gggaggataa actattactg gaccttgcta aaacccggag acacaataat gtttgaggca aatggaaatc taatagcgcc atggtatgct ttcgcactaa gtagaggctt tgggtcaggc atcatcacct caaacgcatc gatgcatgag tgtgacacga agtgtcaaac accccaggga gctataaaca gcagtctccc tttccagaat atacacccag tcacaatagg agagtgccca aaatacgtca ggagtaccaa attgaggatg gttacaggac taaggaacat cccatccatt caatccagag gtctatttgg agccattgcc ggtttcattg aagggggatg gactggaatg atagatggat ggtatggtta tcatcatcag aatgaacagg gatcaggcta tgctgcggat caaaaaagca cacaaaatgc cattaacggg attacaaaca aggtgaactc tgttatcgag aaaatgaaca ctcaattcac agctgtgggt aaagaattca acaacttaga aaaaaggatg gaaaacttaa acaaaaaagt tgatgatgga tttctggaca tttggacata taatgcagaa ttgttggttc tactggaaaa tgaaaggact ctggatttcc atgactcaaa tgtgaagaat ctgtatgaaa aagtaaaaaa ccaattaagg aataatgcca aagaaatagg aaacggatgt tttgagttct accacaagtg taacaatgaa tgcatggaaa gtgtaaaaaa tggaacttat gattatccaa aatattcaga agaatcaaag ttgaacaggg aaaaaataga tggagtgaaa ttggaatcaa tgggggtcta tcagattctg gcgatctact caactgtcgc cagttcactg gtgcttttgg tctccctggg ggcaatcagt ttctggatgt gttctaatgg atctttgcag tgcagaatat gcatctga
Details for InvbU.18715.a.KN11.PD38347
PURIFICATION DATe: 12/19/2018
CONCENTRATION: 9.98mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES, pH 8.5, 150 mM NaCl
EXPRESSION HOST: Tni cells
VIAL COUNT (approx.): 20
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MVLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS DTICIGYHAN NSTDTVDTVL EKNVTVTHSV NLLEDSHNGK LCRLKGIAPL QLGKCNIAGW ILGNPECESL LSERSWSYIV ETPNSENGTC YPGDFIDYEE LREQLSSVSS FERFEIFSKE SSWPKHTTGG VTAACSHAGK SSFYRNLLWL TEKDGSYPNL NNSYVNKKGK EVLVLWGVHH PSNIKDQQTL YQKENAYVSV VSSNYNRRFT PEIAERPKVR GQAGRINYYW TLLKPGDTIM FEANGNLIAP WYAFALSRGF GSGIITSNAS MHECDTKCQT PQGAINSSLP FQNIHPVTIG ECPKYVRSTK LRMVTGLRNI PSIQSRGLFG AIAGFIEGGW TGMIDGWYGY HHQNEQGSGY AADQKSTQNA INGITNKVNS VIEKMNTQFT AVGKEFNNLE KRMENLNKKV DDGFLDIWTY NAELLVLLEN ERTLDFHDSN VKNLYEKVKN QLRNNAKEIG NGCFEFYHKC NNECMESVKN GTYDYPKYSE EFLVPRGSPG SGYIPEAPRD GQAYVRKDGE WVLLSTFLGH HHHHH
Full NT Sequence (Expression Vector + Insert)
GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA GTTGCTGATC AGATCCTCAT TGTTTGCCTC CCTGCTGCGG TTTTTCACCG AAGTTCATGC CAGTCCAGCG TTTTTGCAGC AGAAAAGCCG CCGACTTCGG TTTGCGGTCG CGAGTGAAGA TCCCTTTCTT GTTACCGCCA ACGCGCAATA TGCCTTGCGA GGTCGCAAAA TCGGCGAAAT TCCATACCTG TTCACCGACG ACGGCGCTGA CGCGATCAAA GACGCGGTGA TACATATCCA GCCATGCACA CTGATACTCT TCACTCCACA TGTCGGTGTA CATTGAGTGC AGCCCGGCTA ACGTATCCAC GCCGTATTCG GTGATGATAA TCGGCTGATG CAGTTTCTCC TGCCAGGCCA GAAGTTCTTT TTCCAGTACC TTCTCTGCCG TTTCCAAATC GCCGCTTTGG ACATACCATC CGTAATAACG GTTCAGGCAC AGCACATCAA AGAGATCGCT GATGGTATCG GTGTGAGCGT CGCAGAACAT TACATTGACG CAGGTGATCG GACGCGTCGG GTCGAGTTTA CGCGTTGCTT CCGCCAGTGG CGCGAAATAT TCCCGTGCAC CTTGCGGACG GGTATCCGGT TCGTTGGCAA TACTCCACAT CACCACGCTT GGGTGGTTTT TGTCACGCGC TATCAGCTCT TTAATCGCCT GTAAGTGCGC TTGCTGAGTT TCCCCGTTGA CTGCCTCTTC GCTGTACAGT TCTTTCGGCT TGTTGCCCGC TTCGAAACCA ATGCCTAAAG AGAGCGAAAA GCCGACAGCA GCAGTTTCAT CAATCACCAC GATGCCATGT TCATCTGCCC AGTCGAGCAT CTCTTCAGCG TAAGGGTAAT GCGAGGTACG GTAGGAGTTG GCCCCAATCC AGTCCATTAA TGCGTGGTCG TGCACCATCA GCACGTTATC GAATCCTTTG CCACGTAAGT CCGCATCTTC ATGACGACCA AAGCCAGTAA AGTAGAACGG TTTGTGGTTA ATCAGGAACT GTTGGCCCTT CACTGCCACT GACCGGATGC CGACGCGAAG CGGGTAGATA TCACACTCTG TCTGGCTTTT GGCTGTGACG CACAGTTCAT AGAGATAACC TTCACCCGGT TGCCAGAGGT GCGGATTCAC CACTTGCAAA GTCCCGCTAG TGCCTTGTCC AGTTGCAACC ACCTGTTGAT CCGCATCACG CAGTTCAACG CTGACATCAC CATTGGCCAC CACCTGCCAG TCAACAGACG CGTGGTTACA GTCTTGCGCG ACATGCGTCA CCACGGTGAT ATCGTCCACC CAGGTGTTCG GCGTGGTGTA GAGCATTACG CTGCGATGGA TTCCGGCATA GTTAAAGAAA TCATGGAAGT AAGACTGCTT TTTCTTGCCG TTTTCGTCGG TAATCACCAT TCCCGGCGGG ATAGTCTGCC AGTTCAGTTC GTTGTTCACA CAAACGGTGA TACGTACACT TTTCCCGGCA ATAACATACG GCGTGACATC GGCTTCAAAT GGCGTATAGC CGCCCTGATG CTCCATCACT TCCTGATTAT TGACCCACAC TTTGCCGTAA TGAGTGACCG CATCGAAACG CAGCACGATA CGCTGGCCTG CCCAACCTTT CGGTATAAAG ACTTCGCGCT GATACCAGAC GTTGCCCGCA TAATTACGAA TATCTGCATC GGCGAACTGA TCGTTAAAAC TGCCTGGCAC AGCAATTGCC CGGCTTTCTT GTAACGCGCT TTCCCACCAA CGCTGATCAA TTCCACAGTT TTCGCGATCC AGACTGAATG CCCACAGGCC GTCGAGTTTT TTGATTTCAC GGGTTGGGGT TTCTACAGGA CGGACCATAT TTATAGGTGT AATTTATGTA GCTGTAATTT TTACCTTATT AATATTTTTT ACGCTTTGCA TTCGACGACT GAACTCCCAA ATATATGTTT AACTCGTCTT GGTCGTTTGA ATTTTTGTTG CTGTGTTTCC TAATATTTTC CATCACCTTA AATATGTTAT TGTAATCCTC AATGTTGAAC TTGCAATTGG ACACGGCATA GTTTTCCATA GTCGTGTAAA ACATGGTATT GGCTGCATTG TAATACATCC GACTGAGCGG GTACGGATCT ATGTGTTTGA GCAGCCTGTT CAAAAACTCT GCATCGTCGC AAAACGGAAT TTCGTCGACA GATCTGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT AGGATCTATG GTACTAGTTA ATCAGTCTCA CCAAGGATTC AACAAGGAAC ATACATCAAA GATGGTGTCG GCAATCGTGT TATATGTGCT TCTGGCCGCT GCCGCCCACT CTGCGTTCGC TGGATCCGAC ACCATCTGCA TCGGATACCA CGCCAACAAC TCTACCGACA CCGTCGACAC TGTCCTGGAA AAGAACGTCA CCGTCACTCA CTCTGTCAAC CTGTTGGAGG ACTCTCACAA CGGAAAGCTG TGCAGGCTGA AGGGAATCGC TCCTCTGCAG CTGGGAAAGT GCAACATTGC CGGATGGATC TTGGGAAACC CCGAATGCGA ATCTCTGCTG TCTGAACGCT CTTGGTCTTA CATCGTCGAA ACCCCTAACT CCGAGAACGG AACTTGCTAC CCCGGCGACT TCATCGACTA CGAAGAACTG AGGGAACAGC TGTCCTCTGT CTCTTCTTTC GAACGCTTCG AAATCTTCTC CAAAGAGTCC TCTTGGCCCA AGCACACCAC TGGTGGTGTC ACTGCTGCTT GCTCTCACGC CGGAAAGTCT AGCTTCTACC GCAACCTGCT GTGGCTGACC GAAAAGGACG GATCTTACCC CAACCTGAAC AACTCCTACG TCAACAAGAA GGGCAAAGAA GTCCTGGTCC TCTGGGGAGT CCACCATCCT TCTAACATCA AGGACCAGCA GACCCTGTAC CAGAAAGAAA ACGCCTACGT CAGCGTCGTC AGCTCTAACT ACAACAGGCG CTTCACCCCT GAAATCGCCG AAAGGCCTAA AGTCCGTGGA CAGGCCGGAA GGATCAACTA CTACTGGACC CTGTTGAAGC CCGGCGACAC TATCATGTTC GAAGCCAACG GAAACCTGAT CGCCCCTTGG TACGCTTTCG CTCTGTCTCG CGGATTCGGA TCTGGAATCA TCACCTCTAA CGCCTCTATG CACGAATGCG ACACCAAGTG CCAGACTCCT CAGGGTGCTA TCAACTCTAG CCTGCCTTTC CAGAACATTC ACCCCGTCAC CATCGGAGAA TGCCCCAAAT ACGTCCGCTC TACCAAGCTG AGGATGGTTA CCGGACTGAG GAACATCCCC TCTATCCAGT CTAGGGGACT GTTCGGAGCT ATCGCCGGAT TCATCGAAGG CGGATGGACC GGAATGATCG ACGGATGGTA CGGTTACCAC CACCAGAACG AACAAGGATC TGGATACGCC GCCGACCAGA AGTCTACCCA GAACGCCATC AACGGAATCA CCAACAAAGT CAACTCCGTC ATCGAGAAGA TGAACACCCA GTTCACCGCC GTCGGAAAAG AGTTCAACAA CCTCGAAAAG CGCATGGAAA ACCTCAACAA AAAGGTCGAC GACGGCTTCC TGGACATCTG GACCTACAAC GCTGAACTGC TCGTCCTGCT GGAAAACGAA AGGACCCTGG ACTTCCACGA CTCTAACGTC AAGAACCTCT ACGAGAAAGT GAAGAACCAG CTGCGCAACA ACGCCAAAGA AATCGGAAAC GGATGCTTCG AGTTCTACCA CAAGTGTAAC AACGAGTGCA TGGAATCCGT GAAGAACGGC ACCTACGACT ACCCCAAGTA CTCTGAAGAA TTCCTCGTAC CACGGGGTAG TCCAGGATCT GGATATATCC CGGAGGCCCC AAGAGATGGA CAGGCATACG TGCGGAAGGA TGGCGAATGG GTGCTGTTGT CGACGTTTCT TGGCCATCAT CACCATCACC ACTGATAAGG TCACCACCAC CACCACCACT AAAAGCTTGC GGCCGCACTC GAGCCTAGGT AGCTGAGCGC ATGCAAGCTG ATCCGGGTTA TTAGTACATT TATTAAGCGC TAGATTCTGT GCGTTGTTGA TTTACAGACA ATTGTTGTAC GTATTTTAAT AATTCATTAA ATTTATAATC TTTAGGGTGG TATGTTAGAG CGAAAATCAA ATGATTTTCA GCGTCTTTAT ATCTGAATTT AAATATTAAA TCCTCAATAG ATTTGTAAAA TAGGTTTCGA TTAGTTTCAA ACAAGGGTTG TTTTTCCGAA CCGATGGCTG GACTATCTAA TGGATTTTCG CTCAACGCCA CAAAACTTGC CAAATCTTGT AGCAGCAATC TAGCTTTGTC GATATTCGTT TGTGTTTTGT TTTGTAATAA AGGTTCGACG TCGTTCAAAA TATTATGCGC TTTTGTATTT CTTTCATCAC TGTCGTTAGT GTACAATTGA CTCGACGTAA ACACGTTAAA TAGAGCTTGG ACATATTTAA CATCGGGCGT GTTAGCTTTA TTAGGCCGAT TATCGTCGTC GTCCCAACCC TCGTCGTTAG AAGTTGCTTC CGAAGACGAT TTTGCCATAG CCACACGACG CCTATTAATT GTGTCGGCTA ACACGTCCGC GATCAAATTT GTAGTTGAGC TTTTTGGAAT TATTTCTGAT TGCGGGCGTT TTTGGGCGGG TTTCAATCTA ACTGTGCCCG ATTTTAATTC AGACAACACG TTAGAAAGCG ATGGTGCAGG CGGTGGTAAC ATTTCAGACG GCAAATCTAC TAATGGCGGC GGTGGTGGAG CTGATGATAA ATCTACCATC GGTGGAGGCG CAGGCGGGGC TGGCGGCGGA GGCGGAGGCG GAGGTGGTGG CGGTGATGCA GACGGCGGTT TAGGCTCAAA TGTCTCTTTA GGCAACACAG TCGGCACCTC AACTATTGTA CTGGTTTCGG GCGCCGTTTT TGGTTTGACC GGTCTGAGAC GAGTGCGATT TTTTTCGTTT CTAATAGCTT CCAACAATTG TTGTCTGTCG TCTAAAGGTG CAGCGGGTTG AGGTTCCGTC GGCATTGGTG GAGCGGGCGG CAATTCAGAC ATCGATGGTG GTGGTGGTGG TGGAGGCGCT GGAATGTTAG GCACGGGAGA AGGTGGTGGC GGCGGTGCCG CCGGTATAAT TTGTTCTGGT TTAGTTTGTT CGCGCACGAT TGTGGGCACC GGCGCAGGCG CCGCTGGCTG CACAACGGAA GGTCGTCTGC TTCGAGGCAG CGCTTGGGGT GGTGGCAATT CAATATTATA ATTGGAATAC AAATCGTAAA AATCTGCTAT AAGCATTGTA ATTTCGCTAT CGTTTACCGT GCCGATATTT AACAACCGCT CAATGTAAGC AATTGTATTG TAAAGAGATT GTCTCAAGCT CGGATCGATC CCGCACGCCG ATAACAAGCC TTTTCATTTT TACTACAGCA TTGTAGTGGC GAGACACTTC GCTGTCGTCG AGGTTTAAAC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCA