InvbN.18715.a

Hemagglutinin (HA)

CENTER ID: InvbN.18715.a
ORGANISM: Influenzavirus A Influenza A virus A/Fort Monmouth/1/1947
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
InvbN.18715.a.KN11.PD38312 full length 18 509
InvbN.18715.a.KN11.PD38333 full length 18 509
InvbN.18715.a.TK11.PD38383 full length 65 276

Structures

7JP4
DEPOSITED: 8/7/2020
DETERMINATION: XRay
CLONE: InvbN.18715.a.TK11.GE43114
PROTEIN: InvbN.18715.a.TK11.PD38383
7JPD
DEPOSITED: 8/7/2020
DETERMINATION: XRay
CLONE: InvbN.18715.a.KN11.GE42181
PROTEIN: InvbN.18715.a.KN11.PD38312

Publications by SSGCID

Structural characterisation of hemagglutinin from seven Influenza A H1N1 strains reveal diversity in the C05 antibody recognition site
Abendroth J, Baydo RO, Edwards TE, Lorimer DD, Forwood JK, Conrady DG, Stigliano M, Ghafoori SM, Calhoun BM, Peterson GF, Grice RL
Sci Rep - 2023
volume 13, issue 1, pages 6940
PMID: 37117205; PMCID: PMC10140725

External Resources

RESOURCE REFERENCE ID
IRD: link
UniProt: Q20MG8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKANLLVLLC ALAAADADTI CIGYHANNST DTVDTVLEKN VTVTHSVNLL EDSHNGKLCR LKGIAPLQLG KCNIAGWILG NPECESLLSK RSWSYIAETP NSENGTCYPG DFADYEELRE QLSSVSSFER FEIFPKERSW PKHNITRGVT AACSHAGKSS FYKNLLWLTE TNGSYPKLSK SYVNNKEKEV LVLWGVHHPS NIEDQKTLYR KENAYVSVVS SNYNRRFTPE IAERPKVRGQ AGRMNYYWTL LEPGDTIIFE ANGNLIAPWY AFALSRGFGS GIITSNASMD ECDTKCQTPQ GAINSSLPFQ NIHPVTIGEC PKYVKSTKLR MVTGLRNIPS IQSRGLFGAI AGFIEGGWTG MIDGWYGYHH QNEQGSGYAA DQKSTQNAIN GITNKVNSVI EKMNTQFTAV GKEFNKLEKR MENLNKKVDD GFLDIWTYNA ELLVLLENER TLDFHDSNVK NLYEKVKNQL RNNAKEIGNG CFEFYHKCNN ECMESVKNGT YDYPKYSEES KLNREKIDGV KLESMGVYQI LAIYSTVASS LVLLVSLGAI SFWMCSNGSL QCRICI
NT Sequence
atgaaggcaa acctactggt cctgttatgt gcacttgcag ctgcagatgc agacacaata tgtataggct accatgcgaa caactcaacc gacactgttg acacagtact cgaaaagaat gtgacagtga cacactctgt aaacctactc gaagacagcc acaacgggaa attatgcaga ttaaaaggaa tagccccact acaattgggg aaatgtaaca ttgccggatg gatcttagga aacccagaat gcgaatcact gctttctaag agatcatggt cctacattgc agaaacacca aactctgaga atggaacatg ttacccagga gatttcgccg actatgagga actgagggag caattgagct cagtgtcatc attcgagaga ttcgaaatat tccccaagga aagatcatgg cccaaacaca acataaccag aggagtaacg gcagcatgct cccatgcggg gaaaagcagt ttttacaaaa atttgctctg gctgacggag acaaatggct catacccaaa gctgagcaag tcctatgtga acaataaaga gaaagaagtc cttgtgctat ggggtgttca tcacccgtct aacatagagg atcaaaagac cctctatcgg aaagaaaatg cttatgtctc tgtagtgtct tcaaattata acaggagatt caccccggaa atagcagaaa gacccaaagt aagaggtcaa gcagggagaa tgaactatta ctggactttg ctagaacccg gagacacaat aatatttgag gcaaatggaa atctaatagc gccatggtat gctttcgcac tgagtagagg ctttggatca ggaatcatca cctcaaacgc atcaatggat gaatgtgaca cgaagtgtca aacaccccag ggagctataa acagtagtct cccttttcag aatatacacc cagtcacaat aggagagtgc ccaaaatacg tcaagagtac caaattgagg atggttacag gattaaggaa catcccatcc attcaatcca gaggtctgtt tggagccatt gccggtttca ttgagggggg atggactgga atgatagatg gatggtatgg ttaccatcat cagaatgaac agggatctgg ctatgctgcg gatcaaaaaa gcacacaaaa tgcaattaac gggattacaa ataaggtgaa ctctgttatc gagaaaatga acactcaatt cacagctgtg ggtaaagaat tcaacaaatt agaaaaaaga atggaaaact taaataaaaa agttgatgat ggatttctgg acatttggac atataatgca gaattgttgg ttctactgga aaatgaaagg actttggatt tccatgactc aaatgtgaag aatctgtatg agaaagtaaa aaaccaatta aggaataatg caaaggaaat aggaaacggg tgttttgagt tctaccacaa gtgtaacaat gaatgcatgg aaagtgtaaa aaatggaact tatgattacc caaaatattc agaggaatca aagttaaaca gggaaaaaat tgatggagtg aaattggaat caatgggggt ctatcagatt ctggcgatct actcaactgt cgccagttca ctggtgcttt tggtctccct gggggcaatc agtttctgga tgtgttctaa tggatctttg cagtgcagaa tatgcatctg a
Details for InvbN.18715.a.KN11.PD38312
PURIFICATION DATe: 5/13/2018
CONCENTRATION: 9.84mg/ml
OBSERVED MW: 65kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM Tris pH 8.0, 150 mM NaCl
EXPRESSION HOST: Sf9 cells (ES)
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MVLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS DTICIGYHAN NSTDTVDTVL EKNVTVTHSV NLLEDSHNGK LCRLKGIAPL QLGKCNIAGW ILGNPECESL LSKRSWSYIA ETPNSENGTC YPGDFADYEE LREQLSSVSS FERFEIFPKE RSWPKHNITR GVTAACSHAG KSSFYKNLLW LTETNGSYPK LSKSYVNNKE KEVLVLWGVH HPSNIEDQKT LYRKENAYVS VVSSNYNRRF TPEIAERPKV RGQAGRMNYY WTLLEPGDTI IFEANGNLIA PWYAFALSRG FGSGIITSNA SMDECDTKCQ TPQGAINSSL PFQNIHPVTI GECPKYVKST KLRMVTGLRN IPSIQSRGLF GAIAGFIEGG WTGMIDGWYG YHHQNEQGSG YAADQKSTQN AINGITNKVN SVIEKMNTQF TAVGKEFNKL EKRMENLNKK VDDGFLDIWT YNAELLVLLE NERTLDFHDS NVKNLYEKVK NQLRNNAKEI GNGCFEFYHK CNNECMESVK NGTYDYPKYS EEFLVPRGSP GSGYIPEAPR DGQAYVRKDG EWVLLSTFLG HHHHHH
Full NT Sequence (Expression Vector + Insert)
GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA GTTGCTGATC AGATCCTCAT TGTTTGCCTC CCTGCTGCGG TTTTTCACCG AAGTTCATGC CAGTCCAGCG TTTTTGCAGC AGAAAAGCCG CCGACTTCGG TTTGCGGTCG CGAGTGAAGA TCCCTTTCTT GTTACCGCCA ACGCGCAATA TGCCTTGCGA GGTCGCAAAA TCGGCGAAAT TCCATACCTG TTCACCGACG ACGGCGCTGA CGCGATCAAA GACGCGGTGA TACATATCCA GCCATGCACA CTGATACTCT TCACTCCACA TGTCGGTGTA CATTGAGTGC AGCCCGGCTA ACGTATCCAC GCCGTATTCG GTGATGATAA TCGGCTGATG CAGTTTCTCC TGCCAGGCCA GAAGTTCTTT TTCCAGTACC TTCTCTGCCG TTTCCAAATC GCCGCTTTGG ACATACCATC CGTAATAACG GTTCAGGCAC AGCACATCAA AGAGATCGCT GATGGTATCG GTGTGAGCGT CGCAGAACAT TACATTGACG CAGGTGATCG GACGCGTCGG GTCGAGTTTA CGCGTTGCTT CCGCCAGTGG CGCGAAATAT TCCCGTGCAC CTTGCGGACG GGTATCCGGT TCGTTGGCAA TACTCCACAT CACCACGCTT GGGTGGTTTT TGTCACGCGC TATCAGCTCT TTAATCGCCT GTAAGTGCGC TTGCTGAGTT TCCCCGTTGA CTGCCTCTTC GCTGTACAGT TCTTTCGGCT TGTTGCCCGC TTCGAAACCA ATGCCTAAAG AGAGCGAAAA GCCGACAGCA GCAGTTTCAT CAATCACCAC GATGCCATGT TCATCTGCCC AGTCGAGCAT CTCTTCAGCG TAAGGGTAAT GCGAGGTACG GTAGGAGTTG GCCCCAATCC AGTCCATTAA TGCGTGGTCG TGCACCATCA GCACGTTATC GAATCCTTTG CCACGTAAGT CCGCATCTTC ATGACGACCA AAGCCAGTAA AGTAGAACGG TTTGTGGTTA ATCAGGAACT GTTGGCCCTT CACTGCCACT GACCGGATGC CGACGCGAAG CGGGTAGATA TCACACTCTG TCTGGCTTTT GGCTGTGACG CACAGTTCAT AGAGATAACC TTCACCCGGT TGCCAGAGGT GCGGATTCAC CACTTGCAAA GTCCCGCTAG TGCCTTGTCC AGTTGCAACC ACCTGTTGAT CCGCATCACG CAGTTCAACG CTGACATCAC CATTGGCCAC CACCTGCCAG TCAACAGACG CGTGGTTACA GTCTTGCGCG ACATGCGTCA CCACGGTGAT ATCGTCCACC CAGGTGTTCG GCGTGGTGTA GAGCATTACG CTGCGATGGA TTCCGGCATA GTTAAAGAAA TCATGGAAGT AAGACTGCTT TTTCTTGCCG TTTTCGTCGG TAATCACCAT TCCCGGCGGG ATAGTCTGCC AGTTCAGTTC GTTGTTCACA CAAACGGTGA TACGTACACT TTTCCCGGCA ATAACATACG GCGTGACATC GGCTTCAAAT GGCGTATAGC CGCCCTGATG CTCCATCACT TCCTGATTAT TGACCCACAC TTTGCCGTAA TGAGTGACCG CATCGAAACG CAGCACGATA CGCTGGCCTG CCCAACCTTT CGGTATAAAG ACTTCGCGCT GATACCAGAC GTTGCCCGCA TAATTACGAA TATCTGCATC GGCGAACTGA TCGTTAAAAC TGCCTGGCAC AGCAATTGCC CGGCTTTCTT GTAACGCGCT TTCCCACCAA CGCTGATCAA TTCCACAGTT TTCGCGATCC AGACTGAATG CCCACAGGCC GTCGAGTTTT TTGATTTCAC GGGTTGGGGT TTCTACAGGA CGGACCATAT TTATAGGTGT AATTTATGTA GCTGTAATTT TTACCTTATT AATATTTTTT ACGCTTTGCA TTCGACGACT GAACTCCCAA ATATATGTTT AACTCGTCTT GGTCGTTTGA ATTTTTGTTG CTGTGTTTCC TAATATTTTC CATCACCTTA AATATGTTAT TGTAATCCTC AATGTTGAAC TTGCAATTGG ACACGGCATA GTTTTCCATA GTCGTGTAAA ACATGGTATT GGCTGCATTG TAATACATCC GACTGAGCGG GTACGGATCT ATGTGTTTGA GCAGCCTGTT CAAAAACTCT GCATCGTCGC AAAACGGAAT TTCGTCGACA GATCTGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT AGGATCTATG GTACTAGTTA ATCAGTCTCA CCAAGGATTC AACAAGGAAC ATACATCAAA GATGGTGTCG GCAATCGTGT TATATGTGCT TCTGGCCGCT GCCGCCCACT CTGCGTTCGC TGGATCCGAC ACCATCTGCA TCGGATACCA CGCCAACAAC TCTACCGACA CCGTCGACAC TGTCCTGGAA AAGAACGTCA CCGTCACTCA CTCTGTCAAC CTGTTGGAGG ACTCTCACAA CGGAAAGCTG TGCAGGCTGA AGGGAATCGC TCCTCTGCAG CTGGGAAAGT GCAACATTGC CGGATGGATC TTGGGAAACC CCGAATGCGA ATCTCTGCTG TCTAAGCGCT CTTGGTCTTA CATTGCTGAA ACCCCTAACT CCGAGAACGG AACTTGCTAC CCTGGCGACT TCGCCGACTA CGAAGAACTG AGGGAACAGC TGTCCTCTGT CTCTTCTTTC GAACGCTTCG AAATCTTCCC CAAAGAAAGG TCTTGGCCCA AGCACAACAT CACCAGGGGA GTCACTGCTG CCTGCTCTCA CGCTGGAAAG TCTAGCTTCT ACAAGAACCT GCTGTGGCTG ACCGAGACTA ACGGATCTTA CCCCAAGCTG TCCAAGTCCT ACGTCAACAA CAAAGAGAAA GAGGTCCTGG TCCTCTGGGG AGTCCACCAT CCTTCTAACA TCGAGGACCA AAAGACCCTG TACCGCAAAG AAAACGCCTA CGTCAGCGTC GTCAGCTCTA ACTACAACAG GCGCTTCACC CCTGAAATCG CCGAAAGGCC TAAAGTCCGT GGACAGGCCG GAAGGATGAA CTACTACTGG ACCCTGTTGG AACCCGGCGA CACTATCATC TTCGAAGCCA ACGGAAACCT GATCGCCCCT TGGTACGCTT TCGCTCTGTC TCGCGGATTC GGATCTGGAA TCATCACCTC TAACGCCTCT ATGGACGAAT GCGACACCAA GTGCCAGACT CCTCAGGGTG CTATCAACTC TAGCCTGCCT TTCCAGAACA TTCACCCCGT CACCATCGGA GAATGCCCCA AATACGTCAA GTCTACCAAG CTGAGGATGG TTACCGGACT GAGGAACATC CCCTCTATCC AGTCTAGGGG ACTGTTCGGA GCTATCGCCG GATTCATCGA AGGCGGATGG ACCGGAATGA TCGACGGATG GTACGGTTAC CACCACCAGA ACGAACAAGG ATCTGGATAC GCCGCCGACC AGAAGTCTAC CCAGAACGCC ATCAACGGAA TCACCAACAA AGTCAACTCC GTCATCGAGA AGATGAACAC CCAGTTCACC GCCGTCGGAA AAGAGTTCAA CAAGCTGGAA AAGCGCATGG AAAACCTGAA CAAGAAGGTC GACGACGGAT TCCTGGACAT CTGGACCTAC AACGCCGAAC TGCTGGTCCT GCTGGAAAAC GAAAGGACCC TGGACTTCCA CGACTCTAAC GTCAAGAACC TCTACGAGAA AGTGAAGAAC CAGCTGCGCA ACAACGCCAA AGAAATCGGA AACGGATGCT TCGAGTTCTA CCACAAGTGT AACAACGAGT GCATGGAATC CGTGAAGAAC GGCACCTACG ACTACCCCAA GTACTCTGAA GAATTCCTCG TACCACGGGG TAGTCCAGGA TCTGGATATA TCCCGGAGGC CCCAAGAGAT GGACAGGCAT ACGTGCGGAA GGATGGCGAA TGGGTGCTGT TGTCGACGTT TCTTGGCCAT CATCACCATC ACCACTGATA AGGTCACCAC CACCACCACC ACTAAAAGCT TGCGGCCGCA CTCGAGCCTA GGTAGCTGAG CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCA
Details for InvbN.18715.a.KN11.PD38333
PURIFICATION DATe: 10/24/2018
CONCENTRATION: 8.66mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris pH 8.0, 150mM NaCl, ~250mM Imidazole
EXPRESSION HOST: Sf9 cells (ES)
VIAL COUNT (approx.): 1
VIAL VOLUME: 80µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MVLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS DTICIGYHAN NSTDTVDTVL EKNVTVTHSV NLLEDSHNGK LCRLKGIAPL QLGKCNIAGW ILGNPECESL LSKRSWSYIA ETPNSENGTC YPGDFADYEE LREQLSSVSS FERFEIFPKE RSWPKHNITR GVTAACSHAG KSSFYKNLLW LTETNGSYPK LSKSYVNNKE KEVLVLWGVH HPSNIEDQKT LYRKENAYVS VVSSNYNRRF TPEIAERPKV RGQAGRMNYY WTLLEPGDTI IFEANGNLIA PWYAFALSRG FGSGIITSNA SMDECDTKCQ TPQGAINSSL PFQNIHPVTI GECPKYVKST KLRMVTGLRN IPSIQSRGLF GAIAGFIEGG WTGMIDGWYG YHHQNEQGSG YAADQKSTQN AINGITNKVN SVIEKMNTQF TAVGKEFNKL EKRMENLNKK VDDGFLDIWT YNAELLVLLE NERTLDFHDS NVKNLYEKVK NQLRNNAKEI GNGCFEFYHK CNNECMESVK NGTYDYPKYS EEFLVPRGSP GSGYIPEAPR DGQAYVRKDG EWVLLSTFLG HHHHHH
Full NT Sequence (Expression Vector + Insert)
GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA GTTGCTGATC AGATCCTCAT TGTTTGCCTC CCTGCTGCGG TTTTTCACCG AAGTTCATGC CAGTCCAGCG TTTTTGCAGC AGAAAAGCCG CCGACTTCGG TTTGCGGTCG CGAGTGAAGA TCCCTTTCTT GTTACCGCCA ACGCGCAATA TGCCTTGCGA GGTCGCAAAA TCGGCGAAAT TCCATACCTG TTCACCGACG ACGGCGCTGA CGCGATCAAA GACGCGGTGA TACATATCCA GCCATGCACA CTGATACTCT TCACTCCACA TGTCGGTGTA CATTGAGTGC AGCCCGGCTA ACGTATCCAC GCCGTATTCG GTGATGATAA TCGGCTGATG CAGTTTCTCC TGCCAGGCCA GAAGTTCTTT TTCCAGTACC TTCTCTGCCG TTTCCAAATC GCCGCTTTGG ACATACCATC CGTAATAACG GTTCAGGCAC AGCACATCAA AGAGATCGCT GATGGTATCG GTGTGAGCGT CGCAGAACAT TACATTGACG CAGGTGATCG GACGCGTCGG GTCGAGTTTA CGCGTTGCTT CCGCCAGTGG CGCGAAATAT TCCCGTGCAC CTTGCGGACG GGTATCCGGT TCGTTGGCAA TACTCCACAT CACCACGCTT GGGTGGTTTT TGTCACGCGC TATCAGCTCT TTAATCGCCT GTAAGTGCGC TTGCTGAGTT TCCCCGTTGA CTGCCTCTTC GCTGTACAGT TCTTTCGGCT TGTTGCCCGC TTCGAAACCA ATGCCTAAAG AGAGCGAAAA GCCGACAGCA GCAGTTTCAT CAATCACCAC GATGCCATGT TCATCTGCCC AGTCGAGCAT CTCTTCAGCG TAAGGGTAAT GCGAGGTACG GTAGGAGTTG GCCCCAATCC AGTCCATTAA TGCGTGGTCG TGCACCATCA GCACGTTATC GAATCCTTTG CCACGTAAGT CCGCATCTTC ATGACGACCA AAGCCAGTAA AGTAGAACGG TTTGTGGTTA ATCAGGAACT GTTGGCCCTT CACTGCCACT GACCGGATGC CGACGCGAAG CGGGTAGATA TCACACTCTG TCTGGCTTTT GGCTGTGACG CACAGTTCAT AGAGATAACC TTCACCCGGT TGCCAGAGGT GCGGATTCAC CACTTGCAAA GTCCCGCTAG TGCCTTGTCC AGTTGCAACC ACCTGTTGAT CCGCATCACG CAGTTCAACG CTGACATCAC CATTGGCCAC CACCTGCCAG TCAACAGACG CGTGGTTACA GTCTTGCGCG ACATGCGTCA CCACGGTGAT ATCGTCCACC CAGGTGTTCG GCGTGGTGTA GAGCATTACG CTGCGATGGA TTCCGGCATA GTTAAAGAAA TCATGGAAGT AAGACTGCTT TTTCTTGCCG TTTTCGTCGG TAATCACCAT TCCCGGCGGG ATAGTCTGCC AGTTCAGTTC GTTGTTCACA CAAACGGTGA TACGTACACT TTTCCCGGCA ATAACATACG GCGTGACATC GGCTTCAAAT GGCGTATAGC CGCCCTGATG CTCCATCACT TCCTGATTAT TGACCCACAC TTTGCCGTAA TGAGTGACCG CATCGAAACG CAGCACGATA CGCTGGCCTG CCCAACCTTT CGGTATAAAG ACTTCGCGCT GATACCAGAC GTTGCCCGCA TAATTACGAA TATCTGCATC GGCGAACTGA TCGTTAAAAC TGCCTGGCAC AGCAATTGCC CGGCTTTCTT GTAACGCGCT TTCCCACCAA CGCTGATCAA TTCCACAGTT TTCGCGATCC AGACTGAATG CCCACAGGCC GTCGAGTTTT TTGATTTCAC GGGTTGGGGT TTCTACAGGA CGGACCATAT TTATAGGTGT AATTTATGTA GCTGTAATTT TTACCTTATT AATATTTTTT ACGCTTTGCA TTCGACGACT GAACTCCCAA ATATATGTTT AACTCGTCTT GGTCGTTTGA ATTTTTGTTG CTGTGTTTCC TAATATTTTC CATCACCTTA AATATGTTAT TGTAATCCTC AATGTTGAAC TTGCAATTGG ACACGGCATA GTTTTCCATA GTCGTGTAAA ACATGGTATT GGCTGCATTG TAATACATCC GACTGAGCGG GTACGGATCT ATGTGTTTGA GCAGCCTGTT CAAAAACTCT GCATCGTCGC AAAACGGAAT TTCGTCGACA GATCTGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT AGGATCTATG GTACTAGTTA ATCAGTCTCA CCAAGGATTC AACAAGGAAC ATACATCAAA GATGGTGTCG GCAATCGTGT TATATGTGCT TCTGGCCGCT GCCGCCCACT CTGCGTTCGC TGGATCCGAC ACCATCTGCA TCGGATACCA CGCCAACAAC TCTACCGACA CCGTCGACAC TGTCCTGGAA AAGAACGTCA CCGTCACTCA CTCTGTCAAC CTGTTGGAGG ACTCTCACAA CGGAAAGCTG TGCAGGCTGA AGGGAATCGC TCCTCTGCAG CTGGGAAAGT GCAACATTGC CGGATGGATC TTGGGAAACC CCGAATGCGA ATCTCTGCTG TCTAAGCGCT CTTGGTCTTA CATTGCTGAA ACCCCTAACT CCGAGAACGG AACTTGCTAC CCTGGCGACT TCGCCGACTA CGAAGAACTG AGGGAACAGC TGTCCTCTGT CTCTTCTTTC GAACGCTTCG AAATCTTCCC CAAAGAAAGG TCTTGGCCCA AGCACAACAT CACCAGGGGA GTCACTGCTG CCTGCTCTCA CGCTGGAAAG TCTAGCTTCT ACAAGAACCT GCTGTGGCTG ACCGAGACTA ACGGATCTTA CCCCAAGCTG TCCAAGTCCT ACGTCAACAA CAAAGAGAAA GAGGTCCTGG TCCTCTGGGG AGTCCACCAT CCTTCTAACA TCGAGGACCA AAAGACCCTG TACCGCAAAG AAAACGCCTA CGTCAGCGTC GTCAGCTCTA ACTACAACAG GCGCTTCACC CCTGAAATCG CCGAAAGGCC TAAAGTCCGT GGACAGGCCG GAAGGATGAA CTACTACTGG ACCCTGTTGG AACCCGGCGA CACTATCATC TTCGAAGCCA ACGGAAACCT GATCGCCCCT TGGTACGCTT TCGCTCTGTC TCGCGGATTC GGATCTGGAA TCATCACCTC TAACGCCTCT ATGGACGAAT GCGACACCAA GTGCCAGACT CCTCAGGGTG CTATCAACTC TAGCCTGCCT TTCCAGAACA TTCACCCCGT CACCATCGGA GAATGCCCCA AATACGTCAA GTCTACCAAG CTGAGGATGG TTACCGGACT GAGGAACATC CCCTCTATCC AGTCTAGGGG ACTGTTCGGA GCTATCGCCG GATTCATCGA AGGCGGATGG ACCGGAATGA TCGACGGATG GTACGGTTAC CACCACCAGA ACGAACAAGG ATCTGGATAC GCCGCCGACC AGAAGTCTAC CCAGAACGCC ATCAACGGAA TCACCAACAA AGTCAACTCC GTCATCGAGA AGATGAACAC CCAGTTCACC GCCGTCGGAA AAGAGTTCAA CAAGCTGGAA AAGCGCATGG AAAACCTGAA CAAGAAGGTC GACGACGGAT TCCTGGACAT CTGGACCTAC AACGCCGAAC TGCTGGTCCT GCTGGAAAAC GAAAGGACCC TGGACTTCCA CGACTCTAAC GTCAAGAACC TCTACGAGAA AGTGAAGAAC CAGCTGCGCA ACAACGCCAA AGAAATCGGA AACGGATGCT TCGAGTTCTA CCACAAGTGT AACAACGAGT GCATGGAATC CGTGAAGAAC GGCACCTACG ACTACCCCAA GTACTCTGAA GAATTCCTCG TACCACGGGG TAGTCCAGGA TCTGGATATA TCCCGGAGGC CCCAAGAGAT GGACAGGCAT ACGTGCGGAA GGATGGCGAA TGGGTGCTGT TGTCGACGTT TCTTGGCCAT CATCACCATC ACCACTGATA AGGTCACCAC CACCACCACC ACTAAAAGCT TGCGGCCGCA CTCGAGCCTA GGTAGCTGAG CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCA
Details for InvbN.18715.a.TK11.PD38383
PURIFICATION DATe: 8/5/2019
CONCENTRATION: 5.04mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25mM tris pH 8.5, 150mM NaCl
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 9
VIAL VOLUME: data unavailable
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGLVPRGSA PLQLGKCNIA GWILGNPECE SLLSKRSWSY IAETPNSENG TCYPGDFADY EELREQLSSV SSFERFEIFP KERSWPKHNI TRGVTAACSH AGKSSFYKNL LWLTETNGSY PKLSKSYVNN KEKEVLVLWG VHHPSNIEDQ KTLYRKENAY VSVVSSNYNR RFTPEIAERP KVRGQAGRMN YYWTLLEPGD TIIFEANGNL IAPWYAFALS R
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGATCCGCG CCGCTGCAGC TGGGCAAATG CAACATCGCG GGCTGGATTC TGGGTAACCC GGAGTGCGAA AGCCTGCTGA GCAAGCGTAG CTGGAGCTAC ATTGCGGAGA CCCCGAACAG CGAAAACGGC ACCTGCTACC CGGGTGACTT TGCGGATTAT GAGGAACTGC GTGAGCAACT GAGCAGCGTG AGCAGCTTCG AGCGTTTTGA AATCTTCCCG AAAGAACGTA GCTGGCCGAA GCACAACATT ACCCGTGGTG TTACCGCGGC GTGCAGCCAT GCGGGTAAAA GCAGCTTCTA CAAGAACCTG CTGTGGCTGA CCGAGACCAA CGGCAGCTAC CCGAAGCTGA GCAAAAGCTA TGTGAACAAC AAGGAGAAAG AAGTGCTGGT TCTGTGGGGT GTTCACCACC CGAGCAACAT CGAGGACCAG AAAACCCTGT ACCGTAAGGA AAACGCGTAT GTGAGCGTGG TTAGCAGCAA CTACAACCGT CGTTTTACCC CGGAGATTGC GGAACGTCCG AAAGTTCGTG GCCAAGCGGG TCGTATGAAC TACTATTGGA CCCTGCTGGA GCCGGGTGAT ACCATCATTT TCGAAGCGAA CGGCAACCTG ATCGCGCCGT GGTATGCGTT TGCGCTGAGC CGTTGATAAG CTTGCGGCCG CACTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGAT