InvaN.07057.a
Polymerase acidic protein
| CENTER ID: | InvaN.07057.a |
| ORGANISM: | Influenzavirus A Influenza A virus A/Wilson-Smith/1933 (H1N1) |
| ASSOCIATED DISEASE: | Influenza |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| InvaN.07057.a.D15.PD00269 | full length | 254 | 716 | ||
| InvaN.07057.a.D15.PD00327 | full length | 254 | 716 | unavailable | |
| InvaN.07057.a.ES13.PD00353 | full length | 266 | 717 | ||
| InvaN.07057.a.ES12.PD00354 | full length | 260 | 717 | ||
| InvaN.07057.a.D15.PD00385 | full length | 254 | 716 |
Structures
4IUJ
DEPOSITED: 1/21/2013
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00262
5IEQ
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IF2
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IF5
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IF7
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IF8
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IFB
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IFC
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5IFD
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00269
5V0U
DEPOSITED: 2/28/2017
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00262
6CFP
DEPOSITED: 2/15/2018
DETERMINATION: XRay
CLONE: InvaN.07057.a.D15.GE00044
PROTEIN: InvaN.07057.a.D15.PD00262
Publications by SSGCID
Structural analysis of H1N1 and H7N9 influenza A virus PA in the absence of PB1.
Abendroth J, Barnes SR, Baydo RO, Begley DW, Bullen J, Edwards TE, Fairman JW, Henkel G, Kirkwood JL, Lorimer D, McCormack K, Moen SO, Myler PJ, Phan I, Raymond AC, Stacy R, Staker BL, Tam VC
Sci Rep - 2014
volume 4, issue 4, pages 5944
PMID: 25089892; PMCID: PMC4123200
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| IRD: | link |
| PATRIC ID: | fig|11320.20161.CDS.2 |
| UniProt: | P15659 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD FHFIDEQGES IVVELGDPNA LLKHRFEIIE GRDRTIAWTV INSICNTTGA EKPKFLPDLY DYKKNRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS KEVNARIEPF LKSTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL RSLASWIQNE FNKACELTDS SWIELDEIGE DAAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LTHALR
NT Sequence
atggaagatt ttgtgcgaca atgcttcaat ccgatgattg tcgagcttgc ggaaaaggca atgaaagagt atggagagga cctgaaaatc gaaacaaaca aatttgcagc aatatgcact cacttggaag tgtgcttcat gtattcagat tttcacttca tcgatgagca aggcgagtca atagtcgtag aacttggcga tccaaatgca cttttgaagc acagatttga aataatcgag ggaagagatc gcacaatagc ctggacagta ataaacagta tttgcaacac tacaggggct gagaaaccaa agtttctacc agatttgtat gattacaaga agaatagatt catcgaaatt ggagtaacaa ggagagaagt tcacatatac tatctggaaa aggccaataa aattaaatct gagaagacac acatccacat tttctcattc actggggagg aaatggccac aaaggccgac tacactctcg atgaagaaag cagggctagg atcaaaacca ggctattcac cataagacaa gaaatggcta gcagaggcct ctgggattcc tttcgtcagt ccgagagagg cgaagagaca attgaagaaa gatttgaaat cacaggaaca atgcgcaagc ttgccgacca aagtctcccg ccaaacttct ccagccttga aaattttaga gcctatgtgg atggattcga accgaacggc tacattgagg gcaagctttc tcaaatgtcc aaagaagtaa atgctagaat tgaacctttt ttgaaatcaa caccacgacc acttagactt ccggatgggc ctccctgttc tcagcggtcc aaattcctgc tgatggatgc cttaaaatta agcattgagg acccaagtca tgagggagag gggataccgc tatatgatgc aatcaaatgc atgagaacat tctttggatg gaaggaaccc aatgttgtta aaccacacga aaagggaata aatccaaatt atcttctgtc atggaagcaa gtactggcag aactgcagga cattgagaat gaggagaaaa ttccaaggac taaaaatatg aagaaaacga gtcagttaaa gtgggcactt ggtgagaaca tggcaccaga aaaggtagac tttgacgatt gtaaagatgt aggcgatttg aagcaatatg atagtgatga accagaattg aggtcgcttg caagttggat tcagaatgag ttcaacaagg catgtgaact gaccgattca agctggatag agctcgatga gattggagaa gatgcggctc caattgaaca cattgcaagc atgagaagga attatttcac agcagaggtg tctcattgca gagccacaga atacataatg aagggggtgt acatcaatac tgccttgctt aatgcatcct gtgcagcaat ggatgatttc caattaattc caatgataag caagtgtaga actaaggagg gaaggcgaaa gaccaatttg tacggtttca tcataaaagg aagatcccac ttaaggaatg acaccgatgt ggtaaacttt gtgagcatgg agttttccct cactgaccca agacttgaac cacacaaatg ggagaagtac tgtgttcttg aggtaggaga tatgcttcta agaagtgcca taggccatgt gtcaaggcct atgttcttgt atgtgaggac aaatggaacc tcaaaaatta aaatgaaatg ggggatggaa atgaggcgtt gcctccttca gtcacttcaa caaatcgaga gtatgattga agctgagtcc tctgtcaagg agaaagacat gaccaaagag ttctttgaaa acaaatcaga aacatggccc gttggagagt cccccaaagg agtggaggaa ggttccattg ggaaggtctg cagaacttta ttggcaaagt cggtattcaa cagcttgtat gcatctccac aactggaagg attttcagct gaatcaagaa aactgcttct tatcgttcag gctcttaggg acaacctgga acctgggacc tttgatcttg gggggctata tgaagcaatt gaggagtgcc tgattaatga tccctgggtt ttgcttaatg cttcttggtt caactccttc ctcacacatg cattgagata g
Details for InvaN.07057.a.D15.PD00269
| PURIFICATION DATe: | 3/1/2013 |
| CONCENTRATION: | 20.3mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 77 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 58 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNARI EPFLKSTPRP LRLPDGPPCS QRSKFLLMDA LKLSIEDPSH EGEGIPLYDA IKCMRTFFGW KEPNVVKPHE KGINPNYLLS WKQVLAELQD IENEEKIPRT KNMKKTSQLK WALGENMAPE KVDFDDCKDV GDLKQYDSDE PELRSLASWI QNEFNKACEL TDSSWIELDE IGEXAAPIEH IASMRRNYFT AEVSHCRATE YIMKGVYINT ALLNASXXAM DDXXLXXM
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaaa cgcccgtatc gaaccgttct taaagagcac ccctcgccct ctgcgcctgc cagatggtcc gccgtgtagc cagcgttcca aattcctgct gatggacgca ctgaagctgt ccattgaaga tcctagccac gaaggtgaag gtatccctct gtacgacgca attaaatgca tgcgtacctt ctttggttgg aaggaaccta acgtagttaa acctcacgag aagggcatca acccaaacta cctcctgtct tggaaacagg tgctcgctga actgcaggac atcgaaaacg aagagaaaat tccacgtacc aagaatatga agaagacttc tcagctgaag tgggcactgg gcgaaaacat ggctccggag aaggtcgatt ttgacgactg taaagacgtt ggtgatttga agcaatacga ctctgacgaa ccggaactgc gcagcctcgc atcctggatt cagaacgaat ttaacaaagc atgcgaactg actgatagct cttggatcga actggacgaa atcggcgaag angcagcacc gatcgaacac attgcttcta tgcgccgcaa ctatttcacc gctgaagttt ctcattgtcg tgctaccgaa tacatcatga agggcgttta tatcaacacc gctctcctca acgcttcntg nnctgctatg gacgacttnc nactcnttnc gatgatc
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNARI EPFLKSTPRP LRLPDGPPCS QRSKFLLMDA LKLSIEDPSH EGEGIPLYDA IKCMRTFFGW KEPNVVKPHE KGINPNYLLS WKQVLAELQD IENEEKIPRT KNMKKTSQLK WALGENMAPE KVDFDDCKDV GDLKQYDSDE PELRSLASWI QNEFNKACEL TDSSWIELDE IGEDAAPIEH IASMRRNYFT AEVSHCRATE YIMKGVYINT ALLNASCAAM DDFQLIPMIS KCRTKEGRRK TNLYGFIIKG RSHLRNDTDV VNFVSMEFSL TDPRLEPHKW EKYCVLEVGD MLLRSAIGHV SRPMFLYVRT NGTSKIKMKW GMEMRRCLLQ SLQQIESMIE AESSVKEKDM TKEFFENKSE TWPVGESPKG VEEGSIGKVC RTLLAKSVFN SLYASPQLEG FSAESRKLLL IVQALRDNLE PGTFDLGGLY EAIEECLIND PWVLLNASWF NSFLTHALR
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAA CGCCCGTATC GAACCGTTCT TAAAGAGCAC CCCTCGCCCT CTGCGCCTGC CAGATGGTCC GCCGTGTAGC CAGCGTTCCA AATTCCTGCT GATGGACGCA CTGAAGCTGT CCATTGAAGA TCCTAGCCAC GAAGGTGAAG GTATCCCTCT GTACGACGCA ATTAAATGCA TGCGTACCTT CTTTGGTTGG AAGGAACCTA ACGTAGTTAA ACCTCACGAG AAGGGCATCA ACCCAAACTA CCTCCTGTCT TGGAAACAGG TGCTCGCTGA ACTGCAGGAC ATCGAAAACG AAGAGAAAAT TCCACGTACC AAGAATATGA AGAAGACTTC TCAGCTGAAG TGGGCACTGG GCGAAAACAT GGCTCCGGAG AAGGTCGATT TTGACGACTG TAAAGACGTT GGTGATTTGA AGCAATACGA CTCTGACGAA CCGGAACTGC GCAGCCTCGC ATCCTGGATT CAGAACGAAT TTAACAAAGC ATGCGAACTG ACTGATAGCT CTTGGATCGA ACTGGACGAA ATCGGCGAAG ACGCAGCACC GATCGAACAC ATTGCTTCTA TGCGCCGCAA CTATTTCACC GCTGAAGTTT CTCATTGTCG TGCTACCGAA TACATCATGA AGGGCGTTTA TATCAACACC GCTCTCCTCA ACGCTTCTTG CGCTGCTATG GACGACTTCC AACTCATTCC GATGATCTCC AAGTGCCGCA CTAAAGAAGG CCGCCGCAAG ACTAATCTGT ACGGTTTTAT CATTAAGGGT CGCTCTCACC TCCGCAACGA CACCGACGTC GTGAATTTCG TGTCTATGGA GTTCTCCCTG ACCGACCCGC GTCTGGAGCC GCACAAGTGG GAGAAGTACT GCGTACTTGA AGTTGGCGAT ATGCTGCTCC GCTCCGCAAT CGGCCACGTT TCTCGCCCGA TGTTCCTGTA TGTTCGCACC AACGGCACTT CCAAAATCAA AATGAAATGG GGCATGGAAA TGCGCCGCTG CCTGCTGCAA TCCCTCCAGC AGATTGAGTC TATGATCGAA GCTGAATCCT CTGTAAAAGA AAAGGACATG ACTAAGGAAT TTTTTGAGAA CAAGTCGGAG ACCTGGCCTG TAGGCGAATC TCCTAAGGGC GTCGAAGAAG GCTCCATTGG TAAGGTGTGT CGTACCCTGT TAGCTAAATC CGTGTTCAAC TCCCTGTACG CTTCTCCACA GCTGGAAGGT TTCTCTGCCG AATCTCGTAA ACTGCTCCTG ATCGTGCAGG CTCTCCGTGA CAATCTGGAA CCGGGTACCT TTGACCTGGG CGGCCTGTAT GAAGCTATCG AGGAATGCCT CATCAACGAC CCGTGGGTGC TGCTGAACGC TTCTTGGTTC AATAGCTTTC TCACTCACGC TCTTCGTTAA AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for InvaN.07057.a.D15.PD00327
| PURIFICATION DATe: | 6/26/2014 |
| CONCENTRATION: | 19.77mg/ml |
| OBSERVED MW: | 65kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 58 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNARI EPFLKSTPRP LRLPDGPPCS QRSKFLLMDA LKLSIEDPSH EGEGIPLYDA IKCMRTFFGW KEPNVVKPHE KGINPNYLLS WKQVLAELQD IENEEKIPRT KNMKKTSQLK WALGENMAPE KVDFDDCKDV GDLKQYDSDE PELRSLASWI QNEFNKACEL TDSSWIELDE IGEXAAPIEH IASMRRNYFT AEVSHCRATE YIMKGVYINT ALLNASXXAM DDXXLXXM
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaaa cgcccgtatc gaaccgttct taaagagcac ccctcgccct ctgcgcctgc cagatggtcc gccgtgtagc cagcgttcca aattcctgct gatggacgca ctgaagctgt ccattgaaga tcctagccac gaaggtgaag gtatccctct gtacgacgca attaaatgca tgcgtacctt ctttggttgg aaggaaccta acgtagttaa acctcacgag aagggcatca acccaaacta cctcctgtct tggaaacagg tgctcgctga actgcaggac atcgaaaacg aagagaaaat tccacgtacc aagaatatga agaagacttc tcagctgaag tgggcactgg gcgaaaacat ggctccggag aaggtcgatt ttgacgactg taaagacgtt ggtgatttga agcaatacga ctctgacgaa ccggaactgc gcagcctcgc atcctggatt cagaacgaat ttaacaaagc atgcgaactg actgatagct cttggatcga actggacgaa atcggcgaag angcagcacc gatcgaacac attgcttcta tgcgccgcaa ctatttcacc gctgaagttt ctcattgtcg tgctaccgaa tacatcatga agggcgttta tatcaacacc gctctcctca acgcttcntg nnctgctatg gacgacttnc nactcnttnc gatgatc
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNARI EPFLKSTPRP LRLPDGPPCS QRSKFLLMDA LKLSIEDPSH EGEGIPLYDA IKCMRTFFGW KEPNVVKPHE KGINPNYLLS WKQVLAELQD IENEEKIPRT KNMKKTSQLK WALGENMAPE KVDFDDCKDV GDLKQYDSDE PELRSLASWI QNEFNKACEL TDSSWIELDE IGEDAAPIEH IASMRRNYFT AEVSHCRATE YIMKGVYINT ALLNASCAAM DDFQLIPMIS KCRTKEGRRK TNLYGFIIKG RSHLRNDTDV VNFVSMEFSL TDPRLEPHKW EKYCVLEVGD MLLRSAIGHV SRPMFLYVRT NGTSKIKMKW GMEMRRCLLQ SLQQIESMIE AESSVKEKDM TKEFFENKSE TWPVGESPKG VEEGSIGKVC RTLLAKSVFN SLYASPQLEG FSAESRKLLL IVQALRDNLE PGTFDLGGLY EAIEECLIND PWVLLNASWF NSFLTHALR
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAA CGCCCGTATC GAACCGTTCT TAAAGAGCAC CCCTCGCCCT CTGCGCCTGC CAGATGGTCC GCCGTGTAGC CAGCGTTCCA AATTCCTGCT GATGGACGCA CTGAAGCTGT CCATTGAAGA TCCTAGCCAC GAAGGTGAAG GTATCCCTCT GTACGACGCA ATTAAATGCA TGCGTACCTT CTTTGGTTGG AAGGAACCTA ACGTAGTTAA ACCTCACGAG AAGGGCATCA ACCCAAACTA CCTCCTGTCT TGGAAACAGG TGCTCGCTGA ACTGCAGGAC ATCGAAAACG AAGAGAAAAT TCCACGTACC AAGAATATGA AGAAGACTTC TCAGCTGAAG TGGGCACTGG GCGAAAACAT GGCTCCGGAG AAGGTCGATT TTGACGACTG TAAAGACGTT GGTGATTTGA AGCAATACGA CTCTGACGAA CCGGAACTGC GCAGCCTCGC ATCCTGGATT CAGAACGAAT TTAACAAAGC ATGCGAACTG ACTGATAGCT CTTGGATCGA ACTGGACGAA ATCGGCGAAG ACGCAGCACC GATCGAACAC ATTGCTTCTA TGCGCCGCAA CTATTTCACC GCTGAAGTTT CTCATTGTCG TGCTACCGAA TACATCATGA AGGGCGTTTA TATCAACACC GCTCTCCTCA ACGCTTCTTG CGCTGCTATG GACGACTTCC AACTCATTCC GATGATCTCC AAGTGCCGCA CTAAAGAAGG CCGCCGCAAG ACTAATCTGT ACGGTTTTAT CATTAAGGGT CGCTCTCACC TCCGCAACGA CACCGACGTC GTGAATTTCG TGTCTATGGA GTTCTCCCTG ACCGACCCGC GTCTGGAGCC GCACAAGTGG GAGAAGTACT GCGTACTTGA AGTTGGCGAT ATGCTGCTCC GCTCCGCAAT CGGCCACGTT TCTCGCCCGA TGTTCCTGTA TGTTCGCACC AACGGCACTT CCAAAATCAA AATGAAATGG GGCATGGAAA TGCGCCGCTG CCTGCTGCAA TCCCTCCAGC AGATTGAGTC TATGATCGAA GCTGAATCCT CTGTAAAAGA AAAGGACATG ACTAAGGAAT TTTTTGAGAA CAAGTCGGAG ACCTGGCCTG TAGGCGAATC TCCTAAGGGC GTCGAAGAAG GCTCCATTGG TAAGGTGTGT CGTACCCTGT TAGCTAAATC CGTGTTCAAC TCCCTGTACG CTTCTCCACA GCTGGAAGGT TTCTCTGCCG AATCTCGTAA ACTGCTCCTG ATCGTGCAGG CTCTCCGTGA CAATCTGGAA CCGGGTACCT TTGACCTGGG CGGCCTGTAT GAAGCTATCG AGGAATGCCT CATCAACGAC CCGTGGGTGC TGCTGAACGC TTCTTGGTTC AATAGCTTTC TCACTCACGC TCTTCGTTAA AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for InvaN.07057.a.ES13.PD00353
| PURIFICATION DATe: | 12/15/2014 |
| CONCENTRATION: | 20.2mg/ml |
| OBSERVED MW: | 94kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH ENLYFQGKIE EGKLVIWING DKGYNGLAEV GKKFEKDTGI KVTVEHPDKL EEKFPQVAAT GDGPDIIFWA HDRFGGYAQS GLLAEITPDK AFQDKLYPFT WDAVRYNGKL IAYPIAVEAL SLIYNKDLLP NPPKTWEEIP ALDKELKAKG KSALMFNLQE PYFTWPLIAA DGGYAFKYEN GKYDIKDVGV DNAGAKAGLT FLVDLIKNKH MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTGSRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL RSLASWIQNE FNKACELTDS SWIELDEIGE DAAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LTHALR
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC GAGAACCTTT ATTTCCAAGG CAAAATCGAA GAAGGTAAAC TGGTAATCTG GATTAACGGC GATAAAGGCT ATAACGGTCT CGCTGAAGTC GGTAAGAAAT TCGAGAAAGA TACCGGAATT AAAGTCACCG TTGAGCATCC GGATAAACTG GAAGAGAAAT TCCCACAGGT TGCGGCAACT GGCGATGGCC CTGACATTAT CTTCTGGGCA CACGACCGCT TTGGTGGCTA CGCTCAATCT GGCCTGTTGG CTGAAATCAC CCCGGACAAA GCGTTCCAGG ACAAGCTGTA TCCGTTTACC TGGGATGCCG TACGTTACAA CGGCAAGCTG ATTGCTTACC CGATCGCTGT TGAAGCGTTA TCGCTGATTT ATAACAAAGA TCTGCTGCCG AACCCGCCAA AAACCTGGGA AGAGATCCCG GCGCTGGATA AAGAACTGAA AGCGAAAGGT AAGAGCGCGC TGATGTTCAA CCTGCAAGAA CCGTACTTCA CCTGGCCGCT GATTGCTGCT GACGGGGGTT ATGCGTTCAA GTATGAAAAC GGCAAGTACG ACATTAAAGA CGTGGGCGTG GATAACGCTG GCGCGAAAGC GGGTCTGACC TTCCTGGTTG ACCTGATTAA AAACAAACAC ATGAATGCAG ACACCGATTA CTCCATCGCA GAAGCTGCCT TTAATAAAGG CGAAACAGCG ATGACCATCA ACGGCCCGTG GGCATGGTCC AACATCGACA CCAGCAAAGT GAATTATGGT GTAACGGTAC TGCCGACCTT CAAGGGTCAA CCATCCAAAC CGTTCGTTGG CGTGCTGAGC GCAGGTATTA ACGCCGCCAG TCCGAACAAA GAGCTGGCAA AAGAGTTCCT CGAAAACTAT CTGCTGACTG ATGAAGGTCT GGAAGCGGTT AATAAAGACA AACCGCTGGG TGCCGTAGCG CTGAAGTCTT ACGAGGAAGA GTTGGCGAAA GATCCACGTA TTGCCGCCAC TATGGAAAAC GCCCAGAAAG GTGAAATCAT GCCGAACATC CCGCAGATGT CCGCTTTCTG GTATGCCGTG CGTACTGCGG TGATCAACGC CGCCAGCGGT CGTCAGACTG TCGATGAAGC CCTGAAAGAC GCGCAGACTG GATCCCGCCC TCTGCGCCTG CCAGATGGTC CGCCGTGTAG CCAGCGTTCC AAATTCCTGC TGATGGACGC ACTGAAGCTG TCCATTGAAG ATCCTAGCCA CGAAGGTGAA GGTATCCCTC TGTACGACGC AATTAAATGC ATGCGTACCT TCTTTGGTTG GAAGGAACCT AACGTAGTTA AACCTCACGA GAAGGGCATC AACCCAAACT ACCTCCTGTC TTGGAAACAG GTGCTCGCTG AACTGCAGGA CATCGAAAAC GAAGAGAAAA TTCCACGTAC CAAGAATATG AAGAAGACTT CTCAGCTGAA GTGGGCACTG GGCGAAAACA TGGCTCCGGA GAAGGTCGAT TTTGACGACT GTAAAGACGT TGGTGATTTG AAGCAATACG ACTCTGACGA ACCGGAACTG CGCAGCCTCG CATCCTGGAT TCAGAACGAA TTTAACAAAG CATGCGAACT GACTGATAGC TCTTGGATCG AACTGGACGA AATCGGCGAA GACGCAGCAC CGATCGAACA CATTGCTTCT ATGCGCCGCA ACTATTTCAC CGCTGAAGTT TCTCATTGTC GTGCTACCGA ATACATCATG AAGGGCGTTT ATATCAACAC CGCTCTCCTC AACGCTTCTT GCGCTGCTAT GGACGACTTC CAACTCATTC CGATGATCTC CAAGTGCCGC ACTAAAGAAG GCCGCCGCAA GACTAATCTG TACGGTTTTA TCATTAAGGG TCGCTCTCAC CTCCGCAACG ACACCGACGT CGTGAATTTC GTGTCTATGG AGTTCTCCCT GACCGACCCG CGTCTGGAGC CGCACAAGTG GGAGAAGTAC TGCGTACTTG AAGTTGGCGA TATGCTGCTC CGCTCCGCAA TCGGCCACGT TTCTCGCCCG ATGTTCCTGT ATGTTCGCAC CAACGGCACT TCCAAAATCA AAATGAAATG GGGCATGGAA ATGCGCCGCT GCCTGCTGCA ATCCCTCCAG CAGATTGAGT CTATGATCGA AGCTGAATCC TCTGTAAAAG AAAAGGACAT GACTAAGGAA TTTTTTGAGA ACAAGTCGGA GACCTGGCCT GTAGGCGAAT CTCCTAAGGG CGTCGAAGAA GGCTCCATTG GTAAGGTGTG TCGTACCCTG TTAGCTAAAT CCGTGTTCAA CTCCCTGTAC GCTTCTCCAC AGCTGGAAGG TTTCTCTGCC GAATCTCGTA AACTGCTCCT GATCGTGCAG GCTCTCCGTG ACAATCTGGA ACCGGGTACC TTTGACCTGG GCGGCCTGTA TGAAGCTATC GAGGAATGCC TCATCAACGA CCCGTGGGTG CTGCTGAACG CTTCTTGGTT CAATAGCTTT CTCACTCACG CTCTTCGTTG ATAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for InvaN.07057.a.ES12.PD00354
| PURIFICATION DATe: | 12/18/2014 |
| CONCENTRATION: | 20.4mg/ml |
| OBSERVED MW: | 95kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 47 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH ENLYFQGKIE EGKLVIWING DKGYNGLAEV GKKFEKDTGI KVTVEHPDKL EEKFPQVAAT GDGPDIIFWA HDRFGGYAQS GLLAEITPDK AFQDKLYPFT WDAVRYNGKL IAYPIAVEAL SLIYNKDLLP NPPKTWEEIP ALDKELKAKG KSALMFNLQE PYFTWPLIAA DGGYAFKYEN GKYDIKDVGV DNAGAKAGLT FLVDLIKNKH MNADTDYSIA EAAFNKGETA MTINGPWAWS NIDTSKVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELAK DPRIAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDEALKD AQTGSFLKST PRPLRLPDGP PCSQRSKFLL MDALKLSIED PSHEGEGIPL YDAIKCMRTF FGWKEPNVVK PHEKGINPNY LLSWKQVLAE LQDIENEEKI PRTKNMKKTS QLKWALGENM APEKVDFDDC KDVGDLKQYD SDEPELRSLA SWIQNEFNKA CELTDSSWIE LDEIGEDAAP IEHIASMRRN YFTAEVSHCR ATEYIMKGVY INTALLNASC AAMDDFQLIP MISKCRTKEG RRKTNLYGFI IKGRSHLRND TDVVNFVSME FSLTDPRLEP HKWEKYCVLE VGDMLLRSAI GHVSRPMFLY VRTNGTSKIK MKWGMEMRRC LLQSLQQIES MIEAESSVKE KDMTKEFFEN KSETWPVGES PKGVEEGSIG KVCRTLLAKS VFNSLYASPQ LEGFSAESRK LLLIVQALRD NLEPGTFDLG GLYEAIEECL INDPWVLLNA SWFNSFLTHA LR
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC GAGAACCTTT ATTTCCAAGG CAAAATCGAA GAAGGTAAAC TGGTAATCTG GATTAACGGC GATAAAGGCT ATAACGGTCT CGCTGAAGTC GGTAAGAAAT TCGAGAAAGA TACCGGAATT AAAGTCACCG TTGAGCATCC GGATAAACTG GAAGAGAAAT TCCCACAGGT TGCGGCAACT GGCGATGGCC CTGACATTAT CTTCTGGGCA CACGACCGCT TTGGTGGCTA CGCTCAATCT GGCCTGTTGG CTGAAATCAC CCCGGACAAA GCGTTCCAGG ACAAGCTGTA TCCGTTTACC TGGGATGCCG TACGTTACAA CGGCAAGCTG ATTGCTTACC CGATCGCTGT TGAAGCGTTA TCGCTGATTT ATAACAAAGA TCTGCTGCCG AACCCGCCAA AAACCTGGGA AGAGATCCCG GCGCTGGATA AAGAACTGAA AGCGAAAGGT AAGAGCGCGC TGATGTTCAA CCTGCAAGAA CCGTACTTCA CCTGGCCGCT GATTGCTGCT GACGGGGGTT ATGCGTTCAA GTATGAAAAC GGCAAGTACG ACATTAAAGA CGTGGGCGTG GATAACGCTG GCGCGAAAGC GGGTCTGACC TTCCTGGTTG ACCTGATTAA AAACAAACAC ATGAATGCAG ACACCGATTA CTCCATCGCA GAAGCTGCCT TTAATAAAGG CGAAACAGCG ATGACCATCA ACGGCCCGTG GGCATGGTCC AACATCGACA CCAGCAAAGT GAATTATGGT GTAACGGTAC TGCCGACCTT CAAGGGTCAA CCATCCAAAC CGTTCGTTGG CGTGCTGAGC GCAGGTATTA ACGCCGCCAG TCCGAACAAA GAGCTGGCAA AAGAGTTCCT CGAAAACTAT CTGCTGACTG ATGAAGGTCT GGAAGCGGTT AATAAAGACA AACCGCTGGG TGCCGTAGCG CTGAAGTCTT ACGAGGAAGA GTTGGCGAAA GATCCACGTA TTGCCGCCAC TATGGAAAAC GCCCAGAAAG GTGAAATCAT GCCGAACATC CCGCAGATGT CCGCTTTCTG GTATGCCGTG CGTACTGCGG TGATCAACGC CGCCAGCGGT CGTCAGACTG TCGATGAAGC CCTGAAAGAC GCGCAGACTG GATCCTTCTT AAAGAGCACC CCTCGCCCTC TGCGCCTGCC AGATGGTCCG CCGTGTAGCC AGCGTTCCAA ATTCCTGCTG ATGGACGCAC TGAAGCTGTC CATTGAAGAT CCTAGCCACG AAGGTGAAGG TATCCCTCTG TACGACGCAA TTAAATGCAT GCGTACCTTC TTTGGTTGGA AGGAACCTAA CGTAGTTAAA CCTCACGAGA AGGGCATCAA CCCAAACTAC CTCCTGTCTT GGAAACAGGT GCTCGCTGAA CTGCAGGACA TCGAAAACGA AGAGAAAATT CCACGTACCA AGAATATGAA GAAGACTTCT CAGCTGAAGT GGGCACTGGG CGAAAACATG GCTCCGGAGA AGGTCGATTT TGACGACTGT AAAGACGTTG GTGATTTGAA GCAATACGAC TCTGACGAAC CGGAACTGCG CAGCCTCGCA TCCTGGATTC AGAACGAATT TAACAAAGCA TGCGAACTGA CTGATAGCTC TTGGATCGAA CTGGACGAAA TCGGCGAAGA CGCAGCACCG ATCGAACACA TTGCTTCTAT GCGCCGCAAC TATTTCACCG CTGAAGTTTC TCATTGTCGT GCTACCGAAT ACATCATGAA GGGCGTTTAT ATCAACACCG CTCTCCTCAA CGCTTCTTGC GCTGCTATGG ACGACTTCCA ACTCATTCCG ATGATCTCCA AGTGCCGCAC TAAAGAAGGC CGCCGCAAGA CTAATCTGTA CGGTTTTATC ATTAAGGGTC GCTCTCACCT CCGCAACGAC ACCGACGTCG TGAATTTCGT GTCTATGGAG TTCTCCCTGA CCGACCCGCG TCTGGAGCCG CACAAGTGGG AGAAGTACTG CGTACTTGAA GTTGGCGATA TGCTGCTCCG CTCCGCAATC GGCCACGTTT CTCGCCCGAT GTTCCTGTAT GTTCGCACCA ACGGCACTTC CAAAATCAAA ATGAAATGGG GCATGGAAAT GCGCCGCTGC CTGCTGCAAT CCCTCCAGCA GATTGAGTCT ATGATCGAAG CTGAATCCTC TGTAAAAGAA AAGGACATGA CTAAGGAATT TTTTGAGAAC AAGTCGGAGA CCTGGCCTGT AGGCGAATCT CCTAAGGGCG TCGAAGAAGG CTCCATTGGT AAGGTGTGTC GTACCCTGTT AGCTAAATCC GTGTTCAACT CCCTGTACGC TTCTCCACAG CTGGAAGGTT TCTCTGCCGA ATCTCGTAAA CTGCTCCTGA TCGTGCAGGC TCTCCGTGAC AATCTGGAAC CGGGTACCTT TGACCTGGGC GGCCTGTATG AAGCTATCGA GGAATGCCTC ATCAACGACC CGTGGGTGCT GCTGAACGCT TCTTGGTTCA ATAGCTTTCT CACTCACGCT CTTCGTTGAT AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for InvaN.07057.a.D15.PD00385
| PURIFICATION DATe: | 7/9/2015 |
| CONCENTRATION: | 20.08mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris pH 8.0 200mM NaCl, 1% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 58 |
Protocol Notes
This is Beryllium Batch BOS040119
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNARI EPFLKSTPRP LRLPDGPPCS QRSKFLLMDA LKLSIEDPSH EGEGIPLYDA IKCMRTFFGW KEPNVVKPHE KGINPNYLLS WKQVLAELQD IENEEKIPRT KNMKKTSQLK WALGENMAPE KVDFDDCKDV GDLKQYDSDE PELRSLASWI QNEFNKACEL TDSSWIELDE IGEXAAPIEH IASMRRNYFT AEVSHCRATE YIMKGVYINT ALLNASXXAM DDXXLXXM
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaaa cgcccgtatc gaaccgttct taaagagcac ccctcgccct ctgcgcctgc cagatggtcc gccgtgtagc cagcgttcca aattcctgct gatggacgca ctgaagctgt ccattgaaga tcctagccac gaaggtgaag gtatccctct gtacgacgca attaaatgca tgcgtacctt ctttggttgg aaggaaccta acgtagttaa acctcacgag aagggcatca acccaaacta cctcctgtct tggaaacagg tgctcgctga actgcaggac atcgaaaacg aagagaaaat tccacgtacc aagaatatga agaagacttc tcagctgaag tgggcactgg gcgaaaacat ggctccggag aaggtcgatt ttgacgactg taaagacgtt ggtgatttga agcaatacga ctctgacgaa ccggaactgc gcagcctcgc atcctggatt cagaacgaat ttaacaaagc atgcgaactg actgatagct cttggatcga actggacgaa atcggcgaag angcagcacc gatcgaacac attgcttcta tgcgccgcaa ctatttcacc gctgaagttt ctcattgtcg tgctaccgaa tacatcatga agggcgttta tatcaacacc gctctcctca acgcttcntg nnctgctatg gacgacttnc nactcnttnc gatgatc
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNARI EPFLKSTPRP LRLPDGPPCS QRSKFLLMDA LKLSIEDPSH EGEGIPLYDA IKCMRTFFGW KEPNVVKPHE KGINPNYLLS WKQVLAELQD IENEEKIPRT KNMKKTSQLK WALGENMAPE KVDFDDCKDV GDLKQYDSDE PELRSLASWI QNEFNKACEL TDSSWIELDE IGEDAAPIEH IASMRRNYFT AEVSHCRATE YIMKGVYINT ALLNASCAAM DDFQLIPMIS KCRTKEGRRK TNLYGFIIKG RSHLRNDTDV VNFVSMEFSL TDPRLEPHKW EKYCVLEVGD MLLRSAIGHV SRPMFLYVRT NGTSKIKMKW GMEMRRCLLQ SLQQIESMIE AESSVKEKDM TKEFFENKSE TWPVGESPKG VEEGSIGKVC RTLLAKSVFN SLYASPQLEG FSAESRKLLL IVQALRDNLE PGTFDLGGLY EAIEECLIND PWVLLNASWF NSFLTHALR
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAA CGCCCGTATC GAACCGTTCT TAAAGAGCAC CCCTCGCCCT CTGCGCCTGC CAGATGGTCC GCCGTGTAGC CAGCGTTCCA AATTCCTGCT GATGGACGCA CTGAAGCTGT CCATTGAAGA TCCTAGCCAC GAAGGTGAAG GTATCCCTCT GTACGACGCA ATTAAATGCA TGCGTACCTT CTTTGGTTGG AAGGAACCTA ACGTAGTTAA ACCTCACGAG AAGGGCATCA ACCCAAACTA CCTCCTGTCT TGGAAACAGG TGCTCGCTGA ACTGCAGGAC ATCGAAAACG AAGAGAAAAT TCCACGTACC AAGAATATGA AGAAGACTTC TCAGCTGAAG TGGGCACTGG GCGAAAACAT GGCTCCGGAG AAGGTCGATT TTGACGACTG TAAAGACGTT GGTGATTTGA AGCAATACGA CTCTGACGAA CCGGAACTGC GCAGCCTCGC ATCCTGGATT CAGAACGAAT TTAACAAAGC ATGCGAACTG ACTGATAGCT CTTGGATCGA ACTGGACGAA ATCGGCGAAG ACGCAGCACC GATCGAACAC ATTGCTTCTA TGCGCCGCAA CTATTTCACC GCTGAAGTTT CTCATTGTCG TGCTACCGAA TACATCATGA AGGGCGTTTA TATCAACACC GCTCTCCTCA ACGCTTCTTG CGCTGCTATG GACGACTTCC AACTCATTCC GATGATCTCC AAGTGCCGCA CTAAAGAAGG CCGCCGCAAG ACTAATCTGT ACGGTTTTAT CATTAAGGGT CGCTCTCACC TCCGCAACGA CACCGACGTC GTGAATTTCG TGTCTATGGA GTTCTCCCTG ACCGACCCGC GTCTGGAGCC GCACAAGTGG GAGAAGTACT GCGTACTTGA AGTTGGCGAT ATGCTGCTCC GCTCCGCAAT CGGCCACGTT TCTCGCCCGA TGTTCCTGTA TGTTCGCACC AACGGCACTT CCAAAATCAA AATGAAATGG GGCATGGAAA TGCGCCGCTG CCTGCTGCAA TCCCTCCAGC AGATTGAGTC TATGATCGAA GCTGAATCCT CTGTAAAAGA AAAGGACATG ACTAAGGAAT TTTTTGAGAA CAAGTCGGAG ACCTGGCCTG TAGGCGAATC TCCTAAGGGC GTCGAAGAAG GCTCCATTGG TAAGGTGTGT CGTACCCTGT TAGCTAAATC CGTGTTCAAC TCCCTGTACG CTTCTCCACA GCTGGAAGGT TTCTCTGCCG AATCTCGTAA ACTGCTCCTG ATCGTGCAGG CTCTCCGTGA CAATCTGGAA CCGGGTACCT TTGACCTGGG CGGCCTGTAT GAAGCTATCG AGGAATGCCT CATCAACGAC CCGTGGGTGC TGCTGAACGC TTCTTGGTTC AATAGCTTTC TCACTCACGC TCTTCGTTAA AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT