InvaD.07060.a
Neuraminidase
| CENTER ID: | InvaD.07060.a |
| ORGANISM: | Influenzavirus A Influenza A virus A/California/04/2009 (H1N1) |
| ASSOCIATED DISEASE: | Influenza |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| InvaD.07060.a.D11.GE082212a | full length | 1 | 469 | ||
| InvaD.07060.a.D21.GE082212a | soluble domain 1 | 83 | 469 | ||
| InvaD.07060.a.D31.GE082212a | soluble domain 2 | 95 | 469 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7U2Q
DEPOSITED: 2/24/2022
DETERMINATION: CryoEM
CLONE: InvaD.07060.a.K11.GE44745
PROTEIN: InvaD.07060.a.K11.PE00058
7U2T
DEPOSITED: 2/24/2022
DETERMINATION: CryoEM
CLONE: InvaD.07060.a.K12.GE44746
PROTEIN: InvaD.07060.a.K12.PE00059
Publications by SSGCID
Structure-based design of stabilized recombinant influenza neuraminidase tetramers
Chan S, Veesler D, Park YJ, Lee KK, King NP, Murphy M, Pettie D, Sydeman C, Ellis D, Acton O, Gillespie RA, Ahlrichs M, Borst AJ, Lederhofer J, Tsybovsky Y, Kephart S, Yap C, Creanga A, Olshefsky A, Stephens T, Graham BS, Kanekiyo M
Nat Commun - 2022
volume 13, issue 1825,
PMID: 35383176; PMCID: PMC8983682
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|661120.5.CDS.1 |
| RefSeq: | ACP41107.1 |
| UniProt: | C3W5S3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPNQKIITI GSVCMTIGMA NLILQIGNII SIWISHSIQL GNQNQIETCN QSVITYENNT WVNQTYVNIS NTNFAAGQSV VSVKLAGNSS LCPVSGWAIY SKDNSVRIGS KGDVFVIREP FISCSPLECR TFFLTQGALL NDKHSNGTIK DRSPYRTLMS CPIGEVPSPY NSRFESVAWS ASACHDGINW LTIGISGPDN GAVAVLKYNG IITDTIKSWR NNILRTQESE CACVNGSCFT VMTDGPSNGQ ASYKIFRIEK GKIVKSVEMN APNYHYEECS CYPDSSEITC VCRDNWHGSN RPWVSFNQNL EYQIGYICSG IFGDNPRPND KTGSCGPVSS NGANGVKGFS FKYGNGVWIG RTKSISSRNG FEMIWDPNGW TGTDNNFSIK QDIVGINEWS GYSGSFVQHP ELTGLDCIRP CFWVELIRGR PKENTIWTSG SSISFCGVNS DTVGWSWPDG AELPFTIDK
NT Sequence
atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac agtaaagaca acagtgtaag aatcggttcc aagggggatg tgtttgtcat aagggaacca ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact gtaatgaccg atggaccaag taatggacag gcctcataca agatcttcag aatagaaaag ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt atcactatga ggaatgctcc tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct aatggagcaa atggagtaaa agggttttca ttcaaatacg gcaatggtgt ttggataggg agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca ggatatagcg ggagttttgt tcagcatcca gaactaacag ggctggattg tataagacct tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt gctgagttgc catttaccat tgacaagtaa
Details for InvaD.07060.a.D11.GE082212a
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 9/18/2012 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | data unavailable |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 50 |
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNPN QKIITIGSVC MTIGMANLIL QIGNIISIWI SHSIQLGNQN QIETCNQSVI TYENNTWVNQ TYVNISNTNF AAGQSVVSVK LAGNSSLCPV SGWAIYSKDN SVRIGSKGDV FVIREPFISC SPLECRTFFL TXGALLNDKH SNGTIKXRSP YRTLMSCPIG EVXSPYNSRF ESVAWXASAC XDGIN
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaatcctaac cagaaaatta tcaccatcgg ttctgtgtgt atgaccatcg gtatggcaaa ccttatcctg cagatcggca acatcatcag catctggatt agccactcta tccagttagg caatcagaac cagatcgaaa cctgcaacca atccgtgata acctacgaaa ataacacctg ggttaaccag acttacgtta acatcagcaa caccaacttt gccgctggtc aatccgtagt ttctgtgaaa ctggctggca actcgagcct ctgccctgta tctggctggg ccatctactc taaggacaac tctgtgcgta taggctccaa aggcgacgtt ttcgtgatcc gcgaaccttt catctcttgc agcccgcttg aatgtcgtac cttcttcctc acgcanggcg cactgctgaa cgataagcac tccaacggta ctatcaaaga ncgttcccca taccgtaccc tgatgtcttg cccaatcggt gaagtgncgt ctccgtacaa ctcccgcttt gaatccgtag cttggnccgc atctgcctgc cntgatggca ttaactgg
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNPN QKIITIGSVC MTIGMANLIL QIGNIISIWI SHSIQLGNQN QIETCNQSVI TYENNTWVNQ TYVNISNTNF AAGQSVVSVK LAGNSSLCPV SGWAIYSKDN SVRIGSKGDV FVIREPFISC SPLECRTFFL TQGALLNDKH SNGTIKDRSP YRTLMSCPIG EVPSPYNSRF ESVAWSASAC HDGINWLTIG ISGPDNGAVA VLKYNGIITD TIKSWRNNIL RTQESECACV NGSCFTVMTD GPSNGQASYK IFRIEKGKIV KSVEMNAPNY HYEECSCYPD SSEITCVCRD NWHGSNRPWV SFNQNLEYQI GYICSGIFGD NPRPNDKTGS CGPVSSNGAN GVKGFSFKYG NGVWIGRTKS ISSRNGFEMI WDPNGWTGTD NNFSIKQDIV GINEWSGYSG SFVQHPELTG LDCIRPCFWV ELIRGRPKEN TIWTSGSSIS FCGVNSDTVG WSWPDGAELP FTIDK
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaatcctaac cagaaaatta tcaccatcgg ttctgtgtgt atgaccatcg gtatggcaaa ccttatcctg cagatcggca acatcatcag catctggatt agccactcta tccagttagg caatcagaac cagatcgaaa cctgcaacca atccgtgata acctacgaaa ataacacctg ggttaaccag acttacgtta acatcagcaa caccaacttt gccgctggtc aatccgtagt ttctgtgaaa ctggctggca actcgagcct ctgccctgta tctggctggg ccatctactc taaggacaac tctgtgcgta taggctccaa aggcgacgtt ttcgtgatcc gcgaaccttt catctcttgc agcccgcttg aatgtcgtac cttcttcctc acgcagggcg cactgctgaa cgataagcac tccaacggta ctatcaaaga ccgttcccca taccgtaccc tgatgtcttg cccaatcggt gaagtgccgt ctccgtacaa ctcccgcttt gaatccgtag cttggtccgc atctgcctgc catgatggca ttaactggct gactatcggc atttccggtc ctgataacgg cgcagtggct gttctgaagt acaacggtat aatcaccgac accatcaaaa gctggcgtaa caatatcctg cgtactcagg agagcgagtg tgcttgtgtt aacggcagct gtttcactgt gatgaccgat ggcccttcca acggccaggc aagctacaag atcttccgta tcgagaaggg caaaatcgtt aaatctgttg aaatgaacgc tccgaattat cactacgaag aatgtagctg ctacccggat tcttccgaga taacctgcgt ttgccgtgac aactggcacg gtagcaaccg cccttgggtt tcctttaatc agaacctgga atatcagata ggctatatct gctccggtat tttcggcgac aacccacgtc cgaacgacaa aaccggtagc tgtggccctg tttcctctaa cggtgctaac ggtgtgaagg gtttctcgtt caagtacggt aacggcgttt ggattggtcg tactaaaagc atcagctctc gcaacggttt cgaaatgatt tgggacccta acggttggac cggtaccgac aacaatttca gcatcaagca ggatatagtt ggcatcaacg agtggtccgg ttactctggc tctttcgtgc agcatccgga gttaaccggt ctggactgca tccgtccgtg cttctgggta gagctgatcc gcggccgtcc taaggagaac actatctgga cctctggctc ctctatttcc ttctgcggcg ttaacagcga tactgtgggc tggtcttggc cggacggcgc agagttacca ttcactatcg acaagtaaaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat
Details for InvaD.07060.a.D21.GE082212a
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 9/26/2012 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | data unavailable |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 62 |
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGVKLA GNSSLCPVSG WAIYSKDNSV RIGSKGDVFV IREPFISCSP LECRTFFLTQ GALLNDKHSN GTIKDRSPYR TLMSCPIGEV PSPYNSRFES VAWSASACHD GINWLTIGIS GPDNGAVAVL KYNGIITDTI KSWRNNILRT QESECACVNG SCFTVMTDGP SNGQASYKIF RIEKGKIVKS VEMNAPNYHY XXCSCYPDSS E
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagt gaaactggct ggcaactcga gcctctgccc tgtatctggc tgggccatct actctaagga caactctgtg cgtataggct ccaaaggcga cgttttcgtg atccgcgaac ctttcatctc ttgcagcccg cttgaatgtc gtaccttctt cctcacgcag ggcgcactgc tgaacgataa gcactccaac ggtactatca aagaccgttc cccataccgt accctgatgt cttgcccaat cggtgaagtg ccgtctccgt acaactcccg ctttgaatcc gtagcttggt ccgcatctgc ctgccatgat ggcattaact ggctgactat cggcatttcc ggtcctgata acggcgcagt ggctgttctg aagtacaacg gtataatcac cgacaccatc aaaagctggc gtaacaatat cctgcgtact caggagagcg agtgtgcttg tgttaacggc agctgtttca ctgtgatgac cgatggccct tccaacggcc aggcaagcta caagatcttc cgtatcgaga agggcaaaat cgttaaatct gttgaaatga acgctccgaa ttatcactac naanaatgta gctgctaccc ggattcttcc gaga
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGVKLA GNSSLCPVSG WAIYSKDNSV RIGSKGDVFV IREPFISCSP LECRTFFLTQ GALLNDKHSN GTIKDRSPYR TLMSCPIGEV PSPYNSRFES VAWSASACHD GINWLTIGIS GPDNGAVAVL KYNGIITDTI KSWRNNILRT QESECACVNG SCFTVMTDGP SNGQASYKIF RIEKGKIVKS VEMNAPNYHY EECSCYPDSS EITCVCRDNW HGSNRPWVSF NQNLEYQIGY ICSGIFGDNP RPNDKTGSCG PVSSNGANGV KGFSFKYGNG VWIGRTKSIS SRNGFEMIWD PNGWTGTDNN FSIKQDIVGI NEWSGYSGSF VQHPELTGLD CIRPCFWVEL IRGRPKENTI WTSGSSISFC GVNSDTVGWS WPDGAELPFT IDK
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagt gaaactggct ggcaactcga gcctctgccc tgtatctggc tgggccatct actctaagga caactctgtg cgtataggct ccaaaggcga cgttttcgtg atccgcgaac ctttcatctc ttgcagcccg cttgaatgtc gtaccttctt cctcacgcag ggcgcactgc tgaacgataa gcactccaac ggtactatca aagaccgttc cccataccgt accctgatgt cttgcccaat cggtgaagtg ccgtctccgt acaactcccg ctttgaatcc gtagcttggt ccgcatctgc ctgccatgat ggcattaact ggctgactat cggcatttcc ggtcctgata acggcgcagt ggctgttctg aagtacaacg gtataatcac cgacaccatc aaaagctggc gtaacaatat cctgcgtact caggagagcg agtgtgcttg tgttaacggc agctgtttca ctgtgatgac cgatggccct tccaacggcc aggcaagcta caagatcttc cgtatcgaga agggcaaaat cgttaaatct gttgaaatga acgctccgaa ttatcactac gaagaatgta gctgctaccc ggattcttcc gagataacct gcgtttgccg tgacaactgg cacggtagca accgcccttg ggtttccttt aatcagaacc tggaatatca gataggctat atctgctccg gtattttcgg cgacaaccca cgtccgaacg acaaaaccgg tagctgtggc cctgtttcct ctaacggtgc taacggtgtg aagggtttct cgttcaagta cggtaacggc gtttggattg gtcgtactaa aagcatcagc tctcgcaacg gtttcgaaat gatttgggac cctaacggtt ggaccggtac cgacaacaat ttcagcatca agcaggatat agttggcatc aacgagtggt ccggttactc tggctctttc gtgcagcatc cggagttaac cggtctggac tgcatccgtc cgtgcttctg ggtagagctg atccgcggcc gtcctaagga gaacactatc tggacctctg gctcctctat ttccttctgc ggcgttaaca gcgatactgt gggctggtct tggccggacg gcgcagagtt accattcact atcgacaagt aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for InvaD.07060.a.D31.GE082212a
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 9/25/2012 |
| EXPECTED MW: | 0kDa |
| OBSERVED MW: | data unavailable |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | data unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSGWA IYSKDNSVRI GSKGDVFVIR EPFISCSPLE CRTFFLTQGA LLNDKHSNGT IKDRSPYRTL MSCPIGEVPS PYNSRFESVA WSASACHDGI NWLTIGISGP DNGAVAVLKY NGIITDTIKS WRNNILRTQE SECACVNGSC FTVMTDGPSN GQASYKIFRI EKGKIVKSVE MNAPNYHYEE CSCYPDSSEI TCVCRDNWHG SNRPWVSFNQ NLEYQIGYIC SGIFGDNPRP NDKTGSCGPV SSNGANGVKG FSFKYGNGVW IGRTKSISSR NGFEMIWDPN GWTGTDNNFS IKQDIVGINE WSGYSGSFVQ HPELTGLDCI RPCFWVELIR GRPKENTIWT SGSSISFCGV NSDTVGWSWP DGAELPFTID K
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggatc tggctgggcc atctactcta aggacaactc tgtgcgtata ggctccaaag gcgacgtttt cgtgatccgc gaacctttca tctcttgcag cccgcttgaa tgtcgtacct tcttcctcac gcagggcgca ctgctgaacg ataagcactc caacggtact atcaaagacc gttccccata ccgtaccctg atgtcttgcc caatcggtga agtgccgtct ccgtacaact cccgctttga atccgtagct tggtccgcat ctgcctgcca tgatggcatt aactggctga ctatcggcat ttccggtcct gataacggcg cagtggctgt tctgaagtac aacggtataa tcaccgacac catcaaaagc tggcgtaaca atatcctgcg tactcaggag agcgagtgtg cttgtgttaa cggcagctgt ttcactgtga tgaccgatgg cccttccaac ggccaggcaa gctacaagat cttccgtatc gagaagggca aaatcgttaa atctgttgaa atgaacgctc cgaattatca ctacgaagaa tgtagctgct acccggattc ttccgagata acctgcgttt gccgtgacaa ctggcacggt agcaaccgcc cttgggtttc ctttaatcag aacctggaat atcagatagg ctatatctgc tccggtattt tcggcgacaa cccacgtccg aacgacaaaa ccggtagctg tggccctgtt tcctctaacg gtgctaacgg tgtgaagggt ttctcgttca agtacggtaa cggcgtttgg attggtcgta ctaaaagcat cagctctcgc aacggtttcg aaatgatttg ggaccctaac ggttggaccg gtaccgacaa caatttcagc atcaagcagg atatagttgg catcaacgag tggtccggtt actctggctc tttcgtgcag catccggagt taaccggtct ggactgcatc cgtccgtgct tctgggtaga gctgatccgc ggccgtccta aggagaacac tatctggacc tctggctcct ctatttcctt ctgcggcgtt aacagcgata ctgtgggctg gtcttggccg gacggcgcag agttaccatt cactatcgac aagtaa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSGWA IYSKDNSVRI GSKGDVFVIR EPFISCSPLE CRTFFLTQGA LLNDKHSNGT IKDRSPYRTL MSCPIGEVPS PYNSRFESVA WSASACHDGI NWLTIGISGP DNGAVAVLKY NGIITDTIKS WRNNILRTQE SECACVNGSC FTVMTDGPSN GQASYKIFRI EKGKIVKSVE MNAPNYHYEE CSCYPDSSEI TCVCRDNWHG SNRPWVSFNQ NLEYQIGYIC SGIFGDNPRP NDKTGSCGPV SSNGANGVKG FSFKYGNGVW IGRTKSISSR NGFEMIWDPN GWTGTDNNFS IKQDIVGINE WSGYSGSFVQ HPELTGLDCI RPCFWVELIR GRPKENTIWT SGSSISFCGV NSDTVGWSWP DGAELPFTID K
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggatc tggctgggcc atctactcta aggacaactc tgtgcgtata ggctccaaag gcgacgtttt cgtgatccgc gaacctttca tctcttgcag cccgcttgaa tgtcgtacct tcttcctcac gcagggcgca ctgctgaacg ataagcactc caacggtact atcaaagacc gttccccata ccgtaccctg atgtcttgcc caatcggtga agtgccgtct ccgtacaact cccgctttga atccgtagct tggtccgcat ctgcctgcca tgatggcatt aactggctga ctatcggcat ttccggtcct gataacggcg cagtggctgt tctgaagtac aacggtataa tcaccgacac catcaaaagc tggcgtaaca atatcctgcg tactcaggag agcgagtgtg cttgtgttaa cggcagctgt ttcactgtga tgaccgatgg cccttccaac ggccaggcaa gctacaagat cttccgtatc gagaagggca aaatcgttaa atctgttgaa atgaacgctc cgaattatca ctacgaagaa tgtagctgct acccggattc ttccgagata acctgcgttt gccgtgacaa ctggcacggt agcaaccgcc cttgggtttc ctttaatcag aacctggaat atcagatagg ctatatctgc tccggtattt tcggcgacaa cccacgtccg aacgacaaaa ccggtagctg tggccctgtt tcctctaacg gtgctaacgg tgtgaagggt ttctcgttca agtacggtaa cggcgtttgg attggtcgta ctaaaagcat cagctctcgc aacggtttcg aaatgatttg ggaccctaac ggttggaccg gtaccgacaa caatttcagc atcaagcagg atatagttgg catcaacgag tggtccggtt actctggctc tttcgtgcag catccggagt taaccggtct ggactgcatc cgtccgtgct tctgggtaga gctgatccgc ggccgtccta aggagaacac tatctggacc tctggctcct ctatttcctt ctgcggcgtt aacagcgata ctgtgggctg gtcttggccg gacggcgcag agttaccatt cactatcgac aagtaaaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat