InvaD.07056.a

Polymerase PB1

CENTER ID: InvaD.07056.a
ORGANISM: Influenzavirus A Influenza A virus A/California/04/2009 (H1N1)
ASSOCIATED DISEASE: Influenza
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
InvaD.07056.a.D12.GE082212a full length 26 757
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
IRD: link
IRD: link
IRD: link
PATRIC ID: fig|11320.249889.CDS.1
RefSeq: ACP41103.1
UniProt: C3W5R9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDVNPTLLFL KIPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFENSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MESMNKEEIE ITTHFQRKRR VRDNMTKKMV TQRTIGKKKQ RLNKRGYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNILSMA PIMFSNKMAR LGKGYMFESK RMKIRTQIPA EMLASIDLKY FNESTKKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTIYWWDG LQSSDDFALI VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK KSYINKTGTF EFTSFFYRYG FVANFSMELP SFGVSGVNES ADMSIGVTVI KNNMINNDLG PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFELKKLW DQTQSKVGLL VSDGGPNLYN IRNLHIPEVC LKWELMDDDY RGRLCNPLNP FVSHKEIDSV NNAVVMPAHG PAKSMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS RARIDARVDF ESGRIKKEEF SEIMKICSTI EELRRQK
NT Sequence
atggatgtca atccgactct acttttccta aaaattccag cgcaaaatgc cataagcacc acattccctt atactggaga tcctccatac agccatggaa caggaacagg atacaccatg gacacagtaa acagaacaca ccaatactca gaaaagggaa agtggacgac aaacacagag actggtgcac cccagctcaa cccgattgat ggaccactac ctgaggataa tgaaccaagt gggtatgcac aaacagactg tgttctagag gctatggctt tccttgaaga atcccaccca ggaatatttg agaattcatg ccttgaaaca atggaagttg ttcaacaaac aagggtagat aaactaactc aaggtcgcca gacttatgat tggacattaa acagaaatca accggcagca actgcattgg ccaacaccat agaagtcttt agatcgaatg gcctaacagc taatgagtca ggaaggctaa tagatttctt aaaggatgta atggaatcaa tgaacaaaga ggaaatagag ataacaaccc actttcaaag aaaaaggaga gtaagagaca acatgaccaa gaagatggtc acgcaaagaa caatagggaa gaaaaaacaa agactgaata agagaggcta tctaataaga gcactgacat taaatacgat gaccaaagat gcagagagag gcaagttaaa aagaagggct atcgcaacac ctgggatgca gattagaggt ttcgtatact ttgttgaaac tttagctagg agcatttgcg aaaagcttga acagtctggg ctcccagtag ggggcaatga aaagaaggcc aaactggcaa atgttgtgag aaagatgatg actaattcac aagacacaga gatttctttc acaatcactg gggacaacac taagtggaat gaaaatcaaa atcctcgaat gttcctggcg atgattacat atatcaccag aaatcaaccc gagtggttca gaaacatcct gagcatggca cccataatgt tctcaaacaa aatggcaaga ctagggaaag ggtacatgtt cgagagtaaa agaatgaaga ttcgaacaca aataccagca gaaatgctag caagcattga cctgaagtac ttcaatgaat caacaaagaa gaaaattgag aaaataaggc ctcttctaat agatggcaca gcatcactga gtcctgggat gatgatgggc atgttcaaca tgctaagtac ggtcttggga gtctcgatac tgaatcttgg acaaaagaaa tacaccaaga caatatactg gtgggatggg ctccaatcat ccgacgattt tgctctcata gtgaatgcac caaaccatga gggaatacaa gcaggagtgg acagattcta caggacctgc aagttagtgg gaatcaacat gagcaaaaag aagtcctata taaataagac agggacattt gaattcacaa gcttttttta tcgctatgga tttgtggcta attttagcat ggagctaccc agctttggag tgtctggagt aaatgaatca gctgacatga gtattggagt aacagtgata aagaacaaca tgataaacaa tgaccttgga cctgcaacgg cccagatggc tcttcaattg ttcatcaaag actacagata cacatatagg tgccataggg gagacacaca aattcagacg agaagatcat ttgagttaaa gaagctgtgg gatcaaaccc aatcaaaagt agggctatta gtatcagatg gaggaccaaa cttatacaat atacggaatc ttcacattcc tgaagtctgc ttaaaatggg agctaatgga tgatgattat cggggaagac tttgtaatcc cctgaatccc tttgtcagtc ataaagagat tgattctgta aacaatgctg tggtaatgcc agcccatggt ccagccaaaa gcatggaata tgatgccgtt gcaactacac attcctggat tcccaagagg aatcgttcta ttctcaacac aagccaaagg ggaattcttg aggatgaaca gatgtaccag aagtgctgca atctattcga gaaatttttc cctagcagtt catataggag accggttgga atttctagca tggtggaggc catggtgtct agggcccgga ttgatgccag ggtcgacttc gagtctggac ggatcaagaa agaagagttc tctgagatca tgaagatctg ttccaccatt gaagaactca gacggcaaaa ataa
Details for InvaD.07056.a.D12.GE082212a
HARVESTED ON: data unavailable
SEQUENCED ON: 9/18/2012
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 29
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGGDPP YSHGTGTGYT MDTVNRTHQY SEKGKWTTNT ETGAPQLNPI DGPLPEDNEP SGYAQTDCVL EAMAFLEESH PGIFENSCLE TMEVVQQXRV DKLTXXRQTY XWTLNRNQPA ATALANTIEX FRSXGLTANE XXRLIDFLKD VMXKHX
Validated NT Sequence
cntctannnn nnnttgttnn ntttaagaag gaganatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagg tgatccgccg tactcccacg gcactggcac cggctatact atggacactg taaaccgtac ccaccagtac agcgagaaag gcaagtggac taccaacact gagaccggtg caccgcaact taacccgatc gacggcccac tgcctgagga taacgagccg tccggctacg ctcagaccga ttgcgttctg gaggcaatgg ctttcctgga agaatctcat ccgggtatct tcgagaactc ttgtcttgaa actatggaag tcgtacagca ganccgtgtc gacaaattga ctcnngntcg tcagacctac nactggaccc tgaaccgcaa ccaaccggct gctaccgctc tggctaacac tatcgaantt ttccgctccn atggtctcac cgccaacgag tncnggcgtc tgatcgactt cctgaaagat gtgatggnaa agcatgnata aanaggaaat cgagattacc acccnctttc agcgtaaang tcntgtacnc nanatgacnn nananggnta ctcancnnnn catcngnana nnaannnnnn cngannannc ngntanctga tnnnnncctg acnnnnnnnn ncntgactnn nnnnngnnan ntnannncnn gcatcnntnn ccnnnnnngc nnnncngnnn nnntnnnnnn nnnnnnnnnn nntnnntnnn nnnnaanngn nnnnnnncnn nngnnnngnn nnnnnna
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGGDPP YSHGTGTGYT MDTVNRTHQY SEKGKWTTNT ETGAPQLNPI DGPLPEDNEP SGYAQTDCVL EAMAFLEESH PGIFENSCLE TMEVVQQTRV DKLTQGRQTY DWTLNRNQPA ATALANTIEV FRSNGLTANE SGRLIDFLKD VMESMNKEEI EITTHFQRKR RVRDNMTKKM VTQRTIGKKK QRLNKRGYLI RALTLNTMTK DAERGKLKRR AIATPGMQIR GFVYFVETLA RSICEKLEQS GLPVGGNEKK AKLANVVRKM MTNSQDTEIS FTITGDNTKW NENQNPRMFL AMITYITRNQ PEWFRNILSM APIMFSNKMA RLGKGYMFES KRMKIRTQIP AEMLASIDLK YFNESTKKKI EKIRPLLIDG TASLSPGMMM GMFNMLSTVL GVSILNLGQK KYTKTIYWWD GLQSSDDFAL IVNAPNHEGI QAGVDRFYRT CKLVGINMSK KKSYINKTGT FEFTSFFYRY GFVANFSMEL PSFGVSGVNE SADMSIGVTV IKNNMINNDL GPATAQMALQ LFIKDYRYTY RCHRGDTQIQ TRRSFELKKL WDQTQSKVGL LVSDGGPNLY NIRNLHIPEV CLKWELMDDD YRGRLCNPLN PFVSHKEIDS VNNAVVMPAH GPAKSMEYDA VATTHSWIPK RNRSILNTSQ RGILEDEQMY QKCCNLFEKF FPSSSYRRPV GISSMVEAMV SRARIDARVD FESGRIKKEE FSEIMKICST IEELRRQK
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagg tgatccgccg tactcccacg gcactggcac cggctatact atggacactg taaaccgtac ccaccagtac agcgagaaag gcaagtggac taccaacact gagaccggtg caccgcaact taacccgatc gacggcccac tgcctgagga taacgagccg tccggctacg ctcagaccga ttgcgttctg gaggcaatgg ctttcctgga agaatctcat ccgggtatct tcgagaactc ttgtcttgaa actatggaag tcgtacagca gacccgtgtc gacaaattga ctcagggtcg tcagacctac gactggaccc tgaaccgcaa ccaaccggct gctaccgctc tggctaacac tatcgaagtt ttccgctcca atggtctcac cgccaacgag tccggtcgtc tgatcgactt cctgaaagat gtgatggaaa gcatgaataa agaggaaatc gagattacca cccactttca gcgtaaacgt cgtgtacgcg acaacatgac caagaagatg gttactcagc gcaccatcgg taagaagaaa caacgcctga acaaacgcgg ctacctgatt cgtgccctga ctctcaacac catgactaaa gacgctgagc gtggcaaact caagcgccgt gcaatcgcta ccccgggtat gcagatccgc ggctttgttt acttcgttga aaccttagct cgttccatct gtgagaaact ggaacagtcc ggcctgccgg tgggtggcaa cgagaagaag gctaagctgg ccaacgtggt gcgcaaaatg atgaccaact ctcaggacac cgagatctcc tttactatca ccggtgataa caccaagtgg aatgaaaacc aaaacccgcg tatgttcctc gccatgatca cctatattac tcgcaaccag ccggaatggt ttcgtaacat cctgtctatg gctccgatca tgttttccaa caaaatggct cgtctgggca aaggttacat gttcgaatcc aagcgcatga aaatccgcac tcagatccct gctgaaatgc ttgcctccat cgacctgaaa tactttaacg aatccaccaa gaagaaaatt gagaagattc gtccgctgct gattgacggt actgcttctc tgagcccggg tatgatgatg ggtatgttta acatgctgtc cactgtgttg ggtgtttcta tcctcaactt aggccagaag aagtatacca aaaccatcta ttggtgggat ggtctgcagt ctagcgacga ctttgcactg atcgttaacg ccccgaacca tgagggcatt caggctggtg ttgatcgttt ctaccgtact tgcaaattag ttggtatcaa catgtccaag aagaaatctt acatcaacaa aactggtacc tttgaattta cgtctttctt ttaccgttac ggcttcgtag ctaacttttc tatggagctt ccgagctttg gcgttagcgg tgttaatgaa tccgctgaca tgtccatcgg tgtcaccgtt atcaaaaaca acatgatcaa caacgactta ggccctgcta ctgcacaaat ggcactgcag ctcttcatta aagactaccg ctacacttat cgttgtcacc gtggcgacac tcagatacag acccgtcgta gcttcgaact gaaaaaactc tgggatcaaa cgcagtctaa agtgggcctg ctggtgtctg acggtggccc taacctgtat aacatccgta acttacacat cccggaagtt tgcctgaagt gggagctcat ggacgacgat tatcgcggcc gtctgtgtaa cccactgaac ccattcgtct ctcacaaaga aatcgatagc gtaaacaacg ccgttgtcat gccagctcat ggcccggcta agtctatgga atacgacgca gtagccacca ctcacagctg gattccaaaa cgcaaccgtt ccatcctgaa cacttcccag cgcggcattc tcgaagatga acagatgtat cagaagtgtt gtaacctgtt cgaaaagttc ttcccatcct ctagctaccg ccgcccagtt ggcatctctt ctatggttga agctatggtg tcccgcgccc gtattgatgc tcgcgtcgat ttcgagtctg gtcgtattaa gaaagaagag ttctccgaaa ttatgaaaat ctgttctacc atcgaagaac tgcgtcgcca gaaataaaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat