InvaC.07057.b

Polymerase acid protein (PA)

CENTER ID:	InvaC.07057.b
ORGANISM:	Influenzavirus A influenza A virus A/Equine/Prague/1/1956 (H7N7)
ASSOCIATED DISEASE:	Influenza
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
InvaC.07057.b.D15.GE082212a	InvaC.07057.b		239	716
InvaC.07057.b.D16.GE082212a	InvaC.07057.b		257	716

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
InvaC.07057.b.D15.PD00300	InvaC.07057.b		239	716	unavailable

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEDFVRQCFN PMIVELAEKA MKEYGENPKI ETNKFAAICT HLEVCFMYSD FHFIDDRGES IIVESSDPNV LLKHRFEIIE GRDRTIAWTI VNSICNTTKI EKPRFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS GKTHIHIFSF MGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSEKGEET IEERFEITGT MRRLADQSLP PNFSNLEHFR AYVDGFEPNG FIEGKLSQMS KAVNARIEPF LKATPRPLRL PKGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MKTFFGWKEP KIIKPHEKGI NPNYLLAWKQ TLSEIQDIEN EGKIPKIKNM KKTSQLKWAL GENMAPEKVD FEDCKDISDL KQYSSDEPEQ RSFASWIQNE FNKACELTDS TWIELDEIGE DIAPIEHIAS MRRNYFTAEI SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKSR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL RTAIGQVSRP MFLYIRTNGT SKVKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKTETWP IGESPRGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDIGGLYEAI EECLINDPWV LLNASWFNSF LKHALE

NT Sequence

atggaagatt tcgttcgaca atgtttcaac ccgatgattg ttgaacttgc agaaaaagca atgaaggaat atggagagaa tccaaaaatt gagacaaaca aatttgccgc aatatgcact cacttagagg tgtgcttcat gtactcagac ttccatttca ttgatgatag aggtgaatca ataatcgtgg agtcgagtga tccaaatgtt ctattaaaac atagatttga aataattgaa ggaagggatc gaacaatagc ttggacaata gttaatagta tttgcaatac tacaaaaatt gagaaaccta gattccttcc cgatttatat gattataagg agaacaggtt tattgaaatt ggagtaacac ggagagaagt tcatatctat tatttagaaa aggccaataa aataaaatct ggaaagactc acatccacat cttctcattt atgggagaag aaatggccac taaagctgac tacacattag acgaagaaag ccgagcaaga ataaaaacca gattattcac aataagacaa gaaatggcca gtagaggcct ctgggattcc tttcgccagt ccgaaaaagg tgaagaaaca attgaagaaa gatttgaaat tacagggaca atgcgcaggc ttgctgatca aagcctccca ccaaacttct ccaatctcga gcactttaga gcctatgtgg atgggtttga accaaatggc ttcatagagg gcaaactttc tcaaatgtcc aaagcagtaa acgcaagaat tgagccattc ctaaaagcaa cacctcgccc actcaggtta cctaagggcc caccctgttc ccaacgatcc aaatttttac tgatggatgc cctaaaatta agtattgaag acccaagcca tgagggcgaa gggataccac tatacgatgc aatcaagtgc atgaaaacat tctttggatg gaaagagccc aagatcatca aaccacatga aaagggcata aacccgaact atctattagc ttggaaacaa acactatcag aaatacagga cattgagaat gaggggaaaa tccccaaaat taaaaacatg aaaaaaacaa gtcaactaaa gtgggcactt ggtgagaata tggcaccaga aaaagttgat tttgaggact gcaaagatat cagtgattta aaacaataca gtagtgatga accagagcaa agatcatttg caagctggat ccaaaatgaa ttcaataagg cctgcgaatt aactgactca acctggatag aactcgatga aattggggaa gacattgccc caattgaaca tattgcaagt atgagaagaa actatttcac agcagaaata tctcattgta gagcaactga gtacataatg aaaggagttt atataaatac tgctctatta aacgcttctt gtgcagccat ggatgatttc caactaatcc caatgataag caaatccaga actaaagaag ggagacgaaa aacaaatctg tatgggttca ttataaaagg aaggtcccat ttaagaaatg acactgatgt agtaaatttt gtaagcatgg aattctcttt aacagatcca agacttgaac cacacaaatg ggaaaaatat tgcgttcttg aagtaggaga tatgctttta agaactgcca taggccaggt gtcaagacca atgtttttat acataagaac aaatggaacc tcaaaagtca aaatgaaatg gggaatggaa atgagacgct gtcttcttca atcactccag caaattgaaa gcatgattga agctgaatca tctgtcaaag agaaagacat gacaaaagaa ttcttcgaaa acaaaacaga gacatggcct attggagagt cacccagagg ggtagaggaa ggctctattg ggaaagtctg cagaactctg ttagccaaat cagtatttaa tagtttatat gcatctccac aattagaagg attctccgct gagtccagga aactgcttct tattgtccaa gctctaaggg ataatctaga acctggaact tttgatattg gggggctata tgaagcaatt gaggaatgcc tgattaatga tccctgggtt ttgcttaacg catcttggtt caactccttc cttaaacatg cactagagta g

Details for InvaC.07057.b.D15.GE082212a

HARVESTED ON:	3/9/2010
SEQUENCED ON:	9/26/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	28

Validated AA Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNGFI EGKLSQMSKA VNARIEPFLK ATPRPLRLPK GPPCSXRSKF LLMDALKLSI EDPSHEXEXX PAVRCN

Validated NT Sequence

atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaaa cggcttcatt gaaggcaaac tgagccagat gtcaaaggct gtcaacgctc gcatcgaacc gttccttaaa gccaccccac gcccactccg tctgccgaaa ggcccgccgt gtagccngcg ctctaaattt ctgctgatgg atgcactcaa actcagcatc gaagacccgt cccacgaang cgaangcntc cccgctgtnc gatgcaatta aatgcatgaa aaccttcttt ggttggaaag aaccgaagat catcnaaccg cacgagaagg gtattaaccc gaactacctg ctggnttgg

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNGFI EGKLSQMSKA VNARIEPFLK ATPRPLRLPK GPPCSQRSKF LLMDALKLSI EDPSHEGEGI PLYDAIKCMK TFFGWKEPKI IKPHEKGINP NYLLAWKQTL SEIQDIENEG KIPKIKNMKK TSQLKWALGE NMAPEKVDFE DCKDISDLKQ YSSDEPEQRS FASWIQNEFN KACELTDSTW IELDEIGEDI APIEHIASMR RNYFTAEISH CRATEYIMKG VYINTALLNA SCAAMDDFQL IPMISKSRTK EGRRKTNLYG FIIKGRSHLR NDTDVVNFVS MEFSLTDPRL EPHKWEKYCV LEVGDMLLRT AIGQVSRPMF LYIRTNGTSK VKMKWGMEMR RCLLQSLQQI ESMIEAESSV KEKDMTKEFF ENKTETWPIG ESPRGVEEGS IGKVCRTLLA KSVFNSLYAS PQLEGFSAES RKLLLIVQAL RDNLEPGTFD IGGLYEAIEE CLINDPWVLL NASWFNSFLK HALE

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaaa cggcttcatt gaaggcaaac tgagccagat gtcaaaggct gtcaacgctc gcatcgaacc gttccttaaa gccaccccac gcccactccg tctgccgaaa ggcccgccgt gtagccagcg ctctaaattt ctgctgatgg atgcactcaa actcagcatc gaagacccgt cccacgaagg cgaaggcatc ccgctgtacg atgcaattaa atgcatgaaa accttctttg gttggaaaga accgaagatc atcaaaccgc acgagaaggg tattaacccg aactacctgc tggcttggaa gcagaccctg agcgaaatcc aggacatcga aaacgagggt aaaattccga aaatcaagaa catgaagaag acctcccagc tgaagtgggc tctgggcgaa aacatggccc ctgagaaggt tgacttcgaa gattgcaaag acatctccga tctgaagcag tactcttccg acgaaccaga acagcgtagc ttcgcttcct ggattcagaa cgaatttaac aaagcatgtg agctgaccga cagcacctgg attgaattag acgaaatcgg tgaagatatc gctccaattg aacacatagc tagcatgcgt cgtaactatt tcaccgctga aatctcccat tgtcgtgcca ctgaatacat catgaaaggt gtgtatatca acaccgcact gctgaacgcc tcttgcgcag caatggacga ctttcaactc atcccgatga tctccaagtc tcgcactaaa gaaggtcgtc gcaaaactaa tctgtacggt ttcatcatca aaggccgctc acacctgcgt aacgataccg acgtagttaa cttcgtttct atggaatttt ctttaaccga tccacgcctg gaaccgcaca agtgggaaaa gtattgcgtt ctcgaagttg gtgatatgct gctgcgtacc gctataggcc aagtttcccg tcctatgttt ctgtatattc gtaccaacgg cacctctaaa gttaaaatga agtggggtat ggaaatgcgc cgttgcctcc tgcagtccct tcagcagatc gaatctatga ttgaagctga aagctctgtg aaagagaagg acatgactaa agaattcttc gaaaacaaga ccgagacttg gcctataggc gaaagcccac gtggtgtaga ggaaggctct atcggtaaag tttgtcgtac tctgctggcc aagtctgtct ttaactctct ctacgcttct cctcagctgg aaggcttttc tgctgaatct cgtaagctgc tcctgatcgt gcaggcactc cgtgacaacc tggagccagg cacctttgat atcggcggtc tctacgaagc aatcgaagag tgcctgatca acgatccgtg ggttctgctg aacgcctctt ggttcaactc tttcctgaaa cacgctcttg agtaaaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat

Details for InvaC.07057.b.D16.GE082212a

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/25/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	91

Validated AA Sequence

MEAFAKRQGK EMDSLRFLYD GIRIXADQTP EDLDMEDNDI IEAHREQIGG IEPFLKATPR PLRLPKGPPC SQRSKFLLMD ALKLSIEDPS HEGEGIPLYD AIKCMKTFFG WKEPKIXXPH EKGINPNYLL AWKQXLSEIQ DIENEGKIPK IKNMKKTSQL KWALGENMAX EKXDFEXCKD ISDLXXYSSD EPEQRXFASW IXNXXXXXCE LTDSTXXELD EIXEXIAPIE HIASMRRNYF TAEISHCRAT EYIMKGVYIN TALLNXSCAA MDDFQLIPMI SKSRTKEGRR KTNLYGFIIK GRSHXRNDTD VVNFVSMEFS LTDPRLEPHK WEKYCVLEVG DMLLRTAIGQ VSRPMFLYIR TNGTSKVKMK WGMEMRRCLL QSLQQIESMI EAESSVKEKD MTKEFFENKT ETWPIGESPR GVEEGSIGKV CRTLLAKSVF NSLYASPQLE GFSAESRKLL LIVQALRDNL EPGTFDIGGL YEAIEECLIN DPWVLLNASW FNSFLKHALE

Validated NT Sequence

ttactcaaga gcgtgtttca ggaaagagtt gaaccaagag gcgttcagca gaacccacgg atcgttgatc aggcactctt cgattgcttc gtagagaccg ccgatatcaa aggtgcctgg ctccaggttg tcacggagtg cctgcacgat caggagcagc ttacgagatt cagcagaaaa gccttccagc tgaggagaag cgtagagaga gttaaagaca gacttggcca gcagagtacg acaaacttta ccgatagagc cttcctctac accacgtggg ctttcgccta taggccaagt ctcggtcttg ttttcgaaga attctttagt catgtccttc tctttcacag agctttcagc ttcaatcata gattcgatct gctgaaggga ctgcaggagg caacggcgca tttccatacc ccacttcatt ttaactttag aggtgccgtt ggtacgaata tacagaaaca taggacggga aacttggcct atagcggtac gcagcagcat atcaccaact tcgagaacgc aatacttttc ccacttgtgc ggttccaggc gtggatcggt taaagaaaat tccatagaaa cgaagttaac tacgtcggta tcgttacgca ngtgtgagcg gcctttgatg atgaaaccgt acagattagt tttgcgacga ccttctttag tgcgagactt ggagatcatc gggatgagtt gaaagtcgtc cattgctgcg caaganncgt tcagcagtgc ggtgttgata tacacacctt tcatgatgta ttcagtggca cgacaatggg agatttcagc ggtgaaatag ttacgacgca tgctagctat gtgttcaatt ggagcgatnt nttcacngat ttcgtctaat tcannncagg tgctgtcggt cagctcacat gnttngtnaa nntcgttcng aatccaggaa gcgaagntac gctgttctgg ttcgtcggaa gagtannnnt tcagatcgga gatgtctttg caatnttcga agtcancctt ctcagnggcc atgttttcgc ccagagccca cttcagctgg gaggtcttct tcatgttctt gattttcgga attttaccct cgttttcgat gtcctggatt tcgctcaggn tctgcttcca agccagcagg tagttcgggt taataccctt ctcgtgcggt nnnntgatct tcggttcttt ccaaccaaag aaggttttca tgcatttaat tgcatcgtac agcgggatgc cttcgccttc gtgggacggg tcttcgatgc tgagtttgag tgcatccatc agcagaaatt tagagcgctg gctacacggc gggcctttcg gcagacggag tgggcgtggg gtggctttaa ggaacggttc gattccaccg atttgttcac ggtgtgcttc gataatgtcg ttatcttcca tgtccagatc ttccggagtc tggtctgccn gaatacggat accgtcgtac agaaagcgca gggaatccat ttccttgccc tggcgtttag cgaatgcttc catcaggcga cgcagcgggg tggtcttctt aatcttgaag aagatttcgc tgctaccgtc gctaaccttc aggttgatgt gggtctcc

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGIEPF LKATPRPLRL PKGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MKTFFGWKEP KIIKPHEKGI NPNYLLAWKQ TLSEIQDIEN EGKIPKIKNM KKTSQLKWAL GENMAPEKVD FEDCKDISDL KQYSSDEPEQ RSFASWIQNE FNKACELTDS TWIELDEIGE DIAPIEHIAS MRRNYFTAEI SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKSR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL RTAIGQVSRP MFLYIRTNGT SKVKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKTETWP IGESPRGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDIGGLYEAI EECLINDPWV LLNASWFNSF LKHALE

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat cgaaccgttc cttaaagcca ccccacgccc actccgtctg ccgaaaggcc cgccgtgtag ccagcgctct aaatttctgc tgatggatgc actcaaactc agcatcgaag acccgtccca cgaaggcgaa ggcatcccgc tgtacgatgc aattaaatgc atgaaaacct tctttggttg gaaagaaccg aagatcatca aaccgcacga gaagggtatt aacccgaact acctgctggc ttggaagcag accctgagcg aaatccagga catcgaaaac gagggtaaaa ttccgaaaat caagaacatg aagaagacct cccagctgaa gtgggctctg ggcgaaaaca tggcccctga gaaggttgac ttcgaagatt gcaaagacat ctccgatctg aagcagtact cttccgacga accagaacag cgtagcttcg cttcctggat tcagaacgaa tttaacaaag catgtgagct gaccgacagc acctggattg aattagacga aatcggtgaa gatatcgctc caattgaaca catagctagc atgcgtcgta actatttcac cgctgaaatc tcccattgtc gtgccactga atacatcatg aaaggtgtgt atatcaacac cgcactgctg aacgcctctt gcgcagcaat ggacgacttt caactcatcc cgatgatctc caagtctcgc actaaagaag gtcgtcgcaa aactaatctg tacggtttca tcatcaaagg ccgctcacac ctgcgtaacg ataccgacgt agttaacttc gtttctatgg aattttcttt aaccgatcca cgcctggaac cgcacaagtg ggaaaagtat tgcgttctcg aagttggtga tatgctgctg cgtaccgcta taggccaagt ttcccgtcct atgtttctgt atattcgtac caacggcacc tctaaagtta aaatgaagtg gggtatggaa atgcgccgtt gcctcctgca gtcccttcag cagatcgaat ctatgattga agctgaaagc tctgtgaaag agaaggacat gactaaagaa ttcttcgaaa acaagaccga gacttggcct ataggcgaaa gcccacgtgg tgtagaggaa ggctctatcg gtaaagtttg tcgtactctg ctggccaagt ctgtctttaa ctctctctac gcttctcctc agctggaagg cttttctgct gaatctcgta agctgctcct gatcgtgcag gcactccgtg acaacctgga gccaggcacc tttgatatcg gcggtctcta cgaagcaatc gaagagtgcc tgatcaacga tccgtgggtt ctgctgaacg cctcttggtt caactctttc ctgaaacacg ctcttgagta aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat

Details for InvaC.07057.b.D15.PD00300

PURIFICATION DATe:	10/30/2013
CONCENTRATION:	20.2mg/ml
OBSERVED MW:	65kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	100µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	28

Protocol Notes

notes unavailable

Validated AA Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGNGFI EGKLSQMSKA VNARIEPFLK ATPRPLRLPK GPPCSXRSKF LLMDALKLSI EDPSHEXEXX PAVRCN

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAA CGGCTTCATT GAAGGCAAAC TGAGCCAGAT GTCAAAGGCT GTCAACGCTC GCATCGAACC GTTCCTTAAA GCCACCCCAC GCCCACTCCG TCTGCCGAAA GGCCCGCCGT GTAGCCAGCG CTCTAAATTT CTGCTGATGG ATGCACTCAA ACTCAGCATC GAAGACCCGT CCCACGAAGG CGAAGGCATC CCGCTGTACG ATGCAATTAA ATGCATGAAA ACCTTCTTTG GTTGGAAAGA ACCGAAGATC ATCAAACCGC ACGAGAAGGG TATTAACCCG AACTACCTGC TGGCTTGGAA GCAGACCCTG AGCGAAATCC AGGACATCGA AAACGAGGGT AAAATTCCGA AAATCAAGAA CATGAAGAAG ACCTCCCAGC TGAAGTGGGC TCTGGGCGAA AACATGGCCC CTGAGAAGGT TGACTTCGAA GATTGCAAAG ACATCTCCGA TCTGAAGCAG TACTCTTCCG ACGAACCAGA ACAGCGTAGC TTCGCTTCCT GGATTCAGAA CGAATTTAAC AAAGCATGTG AGCTGACCGA CAGCACCTGG ATTGAATTAG ACGAAATCGG TGAAGATATC GCTCCAATTG AACACATAGC TAGCATGCGT CGTAACTATT TCACCGCTGA AATCTCCCAT TGTCGTGCCA CTGAATACAT CATGAAAGGT GTGTATATCA ACACCGCACT GCTGAACGCC TCTTGCGCAG CAATGGACGA CTTTCAACTC ATCCCGATGA TCTCCAAGTC TCGCACTAAA GAAGGTCGTC GCAAAACTAA TCTGTACGGT TTCATCATCA AAGGCCGCTC ACACCTGCGT AACGATACCG ACGTAGTTAA CTTCGTTTCT ATGGAATTTT CTTTAACCGA TCCACGCCTG GAACCGCACA AGTGGGAAAA GTATTGCGTT CTCGAAGTTG GTGATATGCT GCTGCGTACC GCTATAGGCC AAGTTTCCCG TCCTATGTTT CTGTATATTC GTACCAACGG CACCTCTAAA GTTAAAATGA AGTGGGGTAT GGAAATGCGC CGTTGCCTCC TGCAGTCCCT TCAGCAGATC GAATCTATGA TTGAAGCTGA AAGCTCTGTG AAAGAGAAGG ACATGACTAA AGAATTCTTC GAAAACAAGA CCGAGACTTG GCCTATAGGC GAAAGCCCAC GTGGTGTAGA GGAAGGCTCT ATCGGTAAAG TTTGTCGTAC TCTGCTGGCC AAGTCTGTCT TTAACTCTCT CTACGCTTCT CCTCAGCTGG AAGGCTTTTC TGCTGAATCT CGTAAGCTGC TCCTGATCGT GCAGGCACTC CGTGACAACC TGGAGCCAGG CACCTTTGAT ATCGGCGGTC TCTACGAAGC AATCGAAGAG TGCCTGATCA ACGATCCGTG GGTTCTGCTG AACGCCTCTT GGTTCAACTC TTTCCTGAAA CACGCTCTTG AGTAAAAGCT TGCGGCCGCA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GAT