InvaC.07056.b

Polymerase basic protein 1 (PB1)

CENTER ID:	InvaC.07056.b
ORGANISM:	Influenzavirus A influenza A virus A/Equine/Prague/1/1956 (H7N7)
ASSOCIATED DISEASE:	Influenza
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
InvaC.07056.b.D11.GE082212a	InvaC.07056.b		1	757

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
IRD:	link
PATRIC ID:	fig\|506420.8.CDS.1
UniProt:	D1LRN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDVNPTLLLL KIPVQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS EKGKWTTNTE TGALQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLRESHP GIFEASCLET MEIIQQTRVD KLTQGRQTYD WTLNKNQPAA TAIANTIEVF RSNDLRANES GRLIDFLKDV TLSIDRDEIE ITTHFQRKRR VRDNMTKKMV TQRTIGKKKQ KLNKKSYLIR ALTLNTMTKD AERGKLRRRA IATPGMQIRG FVYFVEALAK SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF TITGDNTKWN ENQNPRVFLT MITYITRNQP EWFRNVLSIA PIMFSNKLAR LGKGYMFESK SMKLRTQIPS EMLASINLKY FNESTRKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTTYWWDG LQSSDDFALI VNAPNPNGIQ AGVDKFYRTC KLVGINMSKK KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGVNES ADMSIGITII KNNMINNDLG PATAQMALQL FIKDYRYTYR CHRGDTQIQT KRTFELKKLW EQTRSKAGLL VSDGGPNLYN IRNLHIPEVC LKWELMDEDY KGRLCNPLNP FVNHKEIESV NNAMVMPAHG PAKSMEYDAV ATTHSWVPKR NRSILNTSQK GILEDENMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGKIKKEEL AEIMKTCSTI EELGRKK

NT Sequence

atggatgtca atccgactct actcttatta aaaataccag tgcaaaatgc cataagcaca acattccctt acactggaga tcctccatac agccatggaa caggaactgg ttacaccatg gacacagtta atagaacaca tcagtactcc gaaaaaggga aatggacaac aaacactgaa actggagcat tacaactgaa tccaattgat ggtccattac ctgaagacaa tgaaccaagt ggatatgcac aaactgattg tgtattggag gctatggctt tccttaggga atcccaccca gggatttttg aggcctcatg tcttgagaca atggaaatta ttcaacaaac aagagtagac aagttgaccc aaggtcgtca gacctatgat tggactttaa ataaaaatca acctgctgca actgcaatag ccaacacgat tgaggtcttc agatcaaatg atttaagagc taacgaatca ggaagactta tagatttcct caaggatgtc actttatcaa tagatagaga tgaaatagaa ataacaacac actttcagag gaaaagaaga gtaagagata acatgaccaa aaaaatggtc acacaaagaa caattgggaa gaaaaaacaa aaattaaaca aaaaaagtta tctgataaga gcattaacac tgaatacaat gacgaaggat gcagaaagag ggaaattgag gcgaagagca atagcaacac caggaatgca aattagaggg tttgtgtatt ttgttgaagc actagctaaa agtatatgtg aaaaacttga gcaatcaggc ctgccagttg gaggaaatga aaaaaaggcc aaattggcta atgttgtaag aaaaatgatg actaattcac aagacactga aatctccttc actatcactg gggataatac taaatggaat gaaaatcaaa atccccgcgt atttttgact atgataacat acataacaag aaaccaacct gaatggttca gaaatgtttt gagtattgca ccaataatgt tctcaaataa attggcaaga ttaggaaaag gatatatgtt tgagagtaaa agtatgaaat tacggacaca gataccatca gaaatgctag caagcattaa tttgaaatat ttcaatgagt caacaagaaa aaagattgag aaaatacgac cactcttaat agatggtaca gcctcattga gccctggaat gatgatgggc atgtttaaca tgctgagcac agttttggga gtttcaattt taaatctagg acagaaaaaa tacacaaaaa ctacatattg gtgggatggc ctccaatcct ctgatgattt cgccttaatt gtgaatgcac ccaaccccaa tgggatacaa gccggagtcg acaagtttta caggacctgt aagctggttg ggatcaatat gagtaaaaag aaatcctata taaacaggac agggacattt gaattcacaa gtttcttcta ccgctatgga tttgttgcaa atttcagcat ggaattgcct agttttggag tatctggggt caatgaatct gctgacatga gtattggaat aacaataata aaaaataaca tgataaacaa tgatcttgga ccagcaactg ctcaaatggc tcttcagcta ttcataaagg actacagata cacataccgc tgccataggg gagacactca aatacaaacc aaaagaacat ttgaactgaa aaaactatgg gaacagactc gttctaaggc aggactatta gtttctgatg gaggaccaaa cttatacaat attagaaacc tccacatccc agaagtctgc ctgaaatggg agttaatgga tgaagattat aaagggagac tatgtaaccc attgaatcca ttcgtcaacc ataaggaaat tgaatcagta aataatgcca tggtaatgcc cgcccatggc cctgccaaaa gcatggaata cgatgcagtt gcaaccacac attcctgggt tcccaaaaga aaccgttcta ttttaaatac aagtcaaaag gggattcttg aggatgaaaa tatgtatcag aaatgctgca acttgttcga aaagtttttc cctagcagct cttacagaag accagttggc atatctagca tggtagaggc catggtttcc agggcccgta ttgatgcacg aattgacttt gagtctggaa agattaagaa ggaggagttg gctgagatca tgaagacctg ttccaccatt gaagagctcg gacggaaaaa atga

Details for InvaC.07056.b.D11.GE082212a

HARVESTED ON:	4/15/2010
SEQUENCED ON:	9/19/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	95
PERCENT COVERAGE:	37

Validated AA Sequence

Validated NT Sequence

ctannnnnat tttgtttaac tttaagaagg agnataccat gggccaccac caccaccacc atagcggcga agtaaaaccg gaagtgaagc cggagaccca catcaacctg aaggttagcg acggtagcag cgaaatcttc ttcaagatta agaagaccac cccgctgcgt cgcctgatgg aagcattcgc taaacgccag ggcaaggaaa tggattccct gcgctttctg tacgacggta tccgtattca ggcagaccag actccggaag atctggacat ggaagataac gacattatcg aagcacaccg tgaacaaatc ggtggaatgg acgtgaaccc gaccctgctg ctgctcaaaa tcccagttca gaacgccatc tccactactt tcccgtatac tggcgatccg ccgtattccc acggcactgg caccggctat actatggaca ccgttaaccg tactcatcag tactctgaga aaggtaagtg gactactaat actgagactg gtgctctgca gctgaacccg atcgatggcc cactcccgga agataacgaa ccgtccggct acgcgcagac cgattgcgtg ctcgaagcta tggcttttct ccgtgagtct cacccgggta tcttcgaagc cagctgtctt gaaactatgg aaatcatcca gcagacccgc gtggacaaac tgacccaggg tcgtcagacg tacgactgga ctctgaacaa gaaccagcca gccgctaccg ccatcgccna caccatcgaa gttttccgct ccaacgatct ccgcgcaaac gaatctggcc gtctgatcga cttccttaaa gatgtgaccc tgtccattga ccgcgacgag atcgaaataa ctactcactt ccagcgtaaa cgccgtgtgc gtgacnacnt gaccaagaan anggttaccc agcgtactat cggcaagaan aaacngaaac tgaacanaan tcctacctna tccgtgncct gacccnnnnn ncntgaccna annnnnnnng cngtaaacnn ntcnnnnnca nagctnctnc ngnntgnnan ncnngntnnn ntantnnnnn annnnnncta nnnnntnnnn nnanncngnn nnnnnnnnnt nncnnnngnn nnannnnnnn nnnnnnnnnn nnnnnnannn nannnnnnnn nnnnnnnnnn nnnnnnctnn a

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMDVN PTLLLLKIPV QNAISTTFPY TGDPPYSHGT GTGYTMDTVN RTHQYSEKGK WTTNTETGAL QLNPIDGPLP EDNEPSGYAQ TDCVLEAMAF LRESHPGIFE ASCLETMEII QQTRVDKLTQ GRQTYDWTLN KNQPAATAIA NTIEVFRSND LRANESGRLI DFLKDVTLSI DRDEIEITTH FQRKRRVRDN MTKKMVTQRT IGKKKQKLNK KSYLIRALTL NTMTKDAERG KLRRRAIATP GMQIRGFVYF VEALAKSICE KLEQSGLPVG GNEKKAKLAN VVRKMMTNSQ DTEISFTITG DNTKWNENQN PRVFLTMITY ITRNQPEWFR NVLSIAPIMF SNKLARLGKG YMFESKSMKL RTQIPSEMLA SINLKYFNES TRKKIEKIRP LLIDGTASLS PGMMMGMFNM LSTVLGVSIL NLGQKKYTKT TYWWDGLQSS DDFALIVNAP NPNGIQAGVD KFYRTCKLVG INMSKKKSYI NRTGTFEFTS FFYRYGFVAN FSMELPSFGV SGVNESADMS IGITIIKNNM INNDLGPATA QMALQLFIKD YRYTYRCHRG DTQIQTKRTF ELKKLWEQTR SKAGLLVSDG GPNLYNIRNL HIPEVCLKWE LMDEDYKGRL CNPLNPFVNH KEIESVNNAM VMPAHGPAKS MEYDAVATTH SWVPKRNRSI LNTSQKGILE DENMYQKCCN LFEKFFPSSS YRRPVGISSM VEAMVSRARI DARIDFESGK IKKEELAEIM KTCSTIEELG RKK

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat ggatgtaaac ccaaccctgt tgctgctcaa aatcccagtt cagaacgcca tctccactac tttcccgtat actggcgatc cgccgtattc ccacggcact ggcaccggct atactatgga caccgttaac cgtactcatc agtactctga gaaaggtaag tggactacta atactgagac tggtgctctg cagctgaacc cgatcgatgg cccactcccg gaagataacg aaccgtccgg ctacgcgcag accgattgcg tgctcgaagc tatggctttt ctccgtgagt ctcacccggg tatcttcgaa gccagctgtc ttgaaactat ggaaatcatc cagcagaccc gcgtggacaa actgacccag ggtcgtcaga cgtacgactg gactctgaac aagaaccagc cagccgctac cgccatcgcc aacaccatcg aagttttccg ctccaacgat ctccgcgcaa acgaatctgg ccgtctgatc gacttcctta aagatgtgac cctgtccatt gaccgcgacg agatcgaaat aactactcac ttccagcgta aacgccgtgt gcgtgacaac atgaccaaga agatggttac ccagcgtact atcggcaaga agaaacagaa actgaacaag aagtcctacc ttatccgtgc cctgaccctt aacaccatga ccaaagacgc ggaacgcggt aaactccgtc gccgtgcaat agctactcca ggcatgcaaa tccgtggctt cgtgtacttt gttgaagcac tcgctaagag catctgcgaa aagctggaac agtcgggtct cccagtaggc ggcaacgaaa agaaagccaa actggccaac gtcgtgcgta agatgatgac caactcccag gatactgaga tctcctttac tatcaccggc gacaacacta aatggaacga gaaccagaat ccacgtgtct tcctgaccat gatcacctac atcacccgta accagccgga atggttccgt aacgtcctgt ctatagctcc gatcatgttc tccaacaaac tggcacgcct gggtaaaggt tacatgttcg aatccaagtc catgaaactg cgtacccaga tcccgtccga aatgctggcc tccatcaacc tgaagtattt caacgagtcc acccgtaaga aaatcgagaa gattcgccca ctgcttatcg acggtactgc tagcctgtct ccgggcatga tgatgggcat gttcaacatg ctgtccaccg ttctcggtgt tagcatttta aacctgggcc agaagaagta cactaaaact acctactggt gggatggcct ccagtcctct gacgatttcg ccttaatcgt taacgcacct aaccctaacg gtatccaggc gggcgttgac aaattctacc gcacctgcaa actggttggc atcaacatga gcaagaagaa gtcgtacatc aaccgcactg gtacctttga atttacttcc ttcttctacc gttacggctt cgtagctaac ttctctatgg aactgccatc cttcggcgtt tccggcgtta atgaatccgc cgacatgtct atcggcatca ccatcatcaa gaataacatg atcaacaacg acttaggccc tgccaccgct cagatggcac tgcaactctt cattaaagat taccgctaca cctaccgttg ccaccgcggc gacacccaga tccaaactaa acgtactttt gaactgaaga agctgtggga acagacacgc tccaaggctg gcctcctggt ttctgacggt ggtccgaacc tttacaacat ccgtaacctg catatcccgg aagtgtgtct gaagtgggaa ctgatggacg aagattataa gggtcgtctc tgcaaccctc tgaacccatt cgtaaaccac aaggaaattg aatctgtgaa caacgctatg gttatgccag ctcacggccc agcaaagagc atggaatacg acgctgtagc taccacccat tcctgggttc cgaaacgcaa ccgttctatt ctgaacacgt cccagaaggg tattctcgaa gatgaaaaca tgtatcagaa gtgttgcaac ctgttcgaga aattcttccc tagctcctct tatcgtcgtc cggttggcat ctcctctatg gttgaagcta tggtatctcg cgctcgtatc gacgctcgca tcgacttcga gagcggcaaa atcaagaaag aagaacttgc agaaattatg aaaacttgtt ccaccatcga agagctgggt cgtaagaaat aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat