InvaB.07057.c

Polymerase acid protein (PA)

CENTER ID: InvaB.07057.c
ORGANISM: Influenzavirus A influenza A virus A/Yokohama/2017/03 (H3N2)
ASSOCIATED DISEASE: Influenza
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
InvaB.07057.c.D12.GE082212a InvaB.07057.c 2 716
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
IRD: link
PATRIC ID: fig|502866.8.CDS.2
UniProt: Q75TA0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD FHFINEQGES IVVELDDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGA EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS ENTHIHIFSF TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMANRGLWDS FRQSERGEET IEEKFEISGT MRRLADQSLP PNFSCLENFR AYVDGFEPNG CIEGKLSQMS KEVNAQIEPF LKTTPRPIKL PNGPPCYQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC IKTFFGWKEP YIVKPHEKGI NSNYLLSWKQ VLSELQDIEN EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FENCRDISDL KQYDSDEPEL RSLSSWIQNE FNKACELTDS IWIELDEIGE DVAPIEYIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL RSAIGQISRP MFLYVRTNGT SKVKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSEAWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLVVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LTHALK
NT Sequence
atggaagatt ttgtgcgaca atgcttcaac ccgatgattg tcgaacttgc agaaaaagca atgaaagagt atggggagga tctgaaaatt gaaacaaaca aatttgcagc aatatgcact cacttggagg tatgtttcat gtattcagat tttcatttca tcaatgaaca aggcgaatca atagtggtag aacttgatga tccaaatgca ctgttaaagc acagatttga aataatcgag gggagagaca gaacaatggc ctggacagta gtaaacagta tctgcaacac tactggagct gaaaaaccga agtttctacc agatttgtat gattacaagg agaacagatt catcgaaatt ggagtgacaa ggagagaagt ccacatatat taccttgaaa aggccaataa gattaaatct gagaacacac acattcacat tttctcattc actggggagg aaatggccac aaaggcagac tacactctcg acgaggaaag cagggctagg attaagacca ggctatttac cataagacaa gaaatggcca acagaggcct ctgggattcc tttcgtcagt ccgaaagagg cgaagaaaca attgaagaaa aatttgaaat ctcaggaact atgcgtaggc ttgccgacca aagtctccca ccgaacttct cctgccttga gaattttaga gcctatgtgg atggattcga accgaacggc tgcattgagg gcaagctttc tcaaatgtcc aaagaagtga atgcccaaat tgaacctttt ctgaagacaa caccaagacc aatcaaactt ccgaatggac ctccttgtta tcagcggtcc aagttcctcc tgatggatgc tttaaaattg agcattgaag acccaagtca cgaaggagaa gggatcccat tatatgatgc gatcaagtgc ataaaaacat tctttggatg gaaagaacct tatatagtca aaccacacga aaagggaata aattcaaatt acctgctgtc atggaagcaa gtattgtcag aattgcagga cattgaaaat gaggagaaga ttccaaggac taaaaacatg aagaaaacga gtcaactaaa gtgggctctt ggtgagaaca tggcaccaga gaaagtagac tttgaaaact gcagagacat aagcgatttg aagcaatatg atagtgacga acctgaatta aggtcacttt caagctggat acagaatgag ttcaacaagg cctgcgagct aactgattca atctggatag agctcgatga aattggagag gacgtagccc caattgaata cattgcaagc atgaggagga attatttcac agcagaggtg tcccattgta gagccactga gtacataatg aagggggtat acattaatac tgccctgctc aatgcatcct gtgcagcaat ggacgatttt caactaattc ccatgataag caagtgcaga actaaagagg gaaggcgaaa aaccaattta tatggattca tcataaaggg aagatctcat ttaaggaatg acacagatgt ggtaaacttt gtgagcatgg agttttctct cactgacccg agacttgagc cacataaatg ggagaaatac tgtgtccttg agataggaga tatgttacta agaagtgcca taggccaaat ttcaaggcct atgttcttgt atgtgaggac aaacggaaca tcaaaggtca aaatgaaatg gggaatggag atgagacgtt gcctccttca gtcactccag cagatcgaga gcatgattga agccgagtcc tcggttaaag agaaagacat gaccaaagag ttttttgaga ataaatcaga agcatggccc attggggagt cccccaaggg agtggaagaa ggttccattg ggaaagtctg taggactcta ttggctaagt cagtgttcaa tagcctgtat gcatcaccac aattggaagg attttcagcg gagtcaagaa aactgctcct tgttgttcag gctcttaggg acaacctcga acctgggacc tttgatcttg gggggctata tgaagcaatt gaggagtgcc tgattaatga tccctgggtt ttgctcaatg cgtcttggtt caactccttc ctgacacatg cattaaaata g
Details for InvaB.07057.c.D12.GE082212a
HARVESTED ON: data unavailable
SEQUENCED ON: 9/25/2012
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 36
Validated AA Sequence
MGHHHHHHSG EVXPEVKPXT HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGEDFV RQCFNPMIVE LAEKAMKEYG EDLKIETNKF AAICTHLEVC FMYSDFHFIN EQGESIVVEL DDXNALLKHR FEIIEGRDRT MAWTVVNSIC NTTGAEKPKF LPDLYDYXEN RFIEIGVTRR EVHIYYLEKA NKXKSENTHI HXFSFTGEXM ATKADYTLDE XSRARIKXRL FTIRXXXANR GLWDSXRQSE XGEXPSREV
Validated NT Sequence
cnnnnnnnnn tttntttaac tttaagaagg agatatacca tgggccacca ccaccaccac catagcggcg aagtanaacc ggaagtgaag ccgganaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggagag gatttcgtgc gccagtgctt taacccgatg atcgttgaac tggcggagaa ggcaatgaaa gaatacggcg aagatctgaa aattgagacc aacaaattcg ctgctatctg cacccacctc gaagtttgct tcatgtactc cgacttccac ttcatcaatg agcagggtga gagcatcgtg gtagaactgg acgatnnnaa cgccctcctg aagcaccgct tcgagatcat tgaaggccgc gatcgcacta tggcttggac ggtagttaac tccatctgca acaccaccgg cgctgaaaag ccgaagttcc tgccagatct ctatgattac naggaaaacc gtttcatcga aataggcgtt actcgtcgtg aggttcatat ctactacctg gaaaaggcta acaaaancaa aagcgaaaac acccacatcc acntcttctc ctttaccggt gaannaatgg caactaaagc tgactacacc ctcgatgaan aatcacgtgc ccgcatcaaa nnccgtctgt tcaccatccg tcnngnantg gctaatcgtg gcctctggga ctccntncgt caatctgaac ntggcgaana accatcgaga gaagtttgaa atttcnnnna ccntgcgtcg ctnagctgat cagtctctgc cgncnaantt ttctngcntc naaaacttnc gnnntnnnnn tnatggnttn naancnaang gctgtnntng nnngnnagcn ntncccanna annnnnnnnn nnnancnntn cntnnnnnnn nnnnnnnnnn nnnngnnncn nannnccnnc nngcnnnnnn nnnnnnaann nnnctnannn nnncnnnnna nncnnnnnnn nnnnnnnngc nnnnnnnnnn nnnnaaannn nnnnnnng
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGEDFV RQCFNPMIVE LAEKAMKEYG EDLKIETNKF AAICTHLEVC FMYSDFHFIN EQGESIVVEL DDPNALLKHR FEIIEGRDRT MAWTVVNSIC NTTGAEKPKF LPDLYDYKEN RFIEIGVTRR EVHIYYLEKA NKIKSENTHI HIFSFTGEEM ATKADYTLDE ESRARIKTRL FTIRQEMANR GLWDSFRQSE RGEETIEEKF EISGTMRRLA DQSLPPNFSC LENFRAYVDG FEPNGCIEGK LSQMSKEVNA QIEPFLKTTP RPIKLPNGPP CYQRSKFLLM DALKLSIEDP SHEGEGIPLY DAIKCIKTFF GWKEPYIVKP HEKGINSNYL LSWKQVLSEL QDIENEEKIP RTKNMKKTSQ LKWALGENMA PEKVDFENCR DISDLKQYDS DEPELRSLSS WIQNEFNKAC ELTDSIWIEL DEIGEDVAPI EYIASMRRNY FTAEVSHCRA TEYIMKGVYI NTALLNASCA AMDDFQLIPM ISKCRTKEGR RKTNLYGFII KGRSHLRNDT DVVNFVSMEF SLTDPRLEPH KWEKYCVLEI GDMLLRSAIG QISRPMFLYV RTNGTSKVKM KWGMEMRRCL LQSLQQIESM IEAESSVKEK DMTKEFFENK SEAWPIGESP KGVEEGSIGK VCRTLLAKSV FNSLYASPQL EGFSAESRKL LLVVQALRDN LEPGTFDLGG LYEAIEECLI NDPWVLLNAS WFNSFLTHAL K
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaga ggatttcgtg cgccagtgct ttaacccgat gatcgttgaa ctggcggaga aggcaatgaa agaatacggc gaagatctga aaattgagac caacaaattc gctgctatct gcacccacct cgaagtttgc ttcatgtact ccgacttcca cttcatcaat gagcagggtg agagcatcgt ggtagaactg gacgatccga acgccctcct gaagcaccgc ttcgagatca ttgaaggccg cgatcgcact atggcttgga cggtagttaa ctccatctgc aacaccaccg gcgctgaaaa gccgaagttc ctgccagatc tctatgatta caaggaaaac cgtttcatcg aaataggcgt tactcgtcgt gaggttcata tctactacct ggaaaaggct aacaaaatca aaagcgaaaa cacccacatc cacatcttct cctttaccgg tgaagaaatg gcaactaaag ctgactacac cctcgatgaa gaatcacgtg cccgcatcaa aacccgtctg ttcaccatcc gtcaggaaat ggctaatcgt ggcctctggg actccttccg tcaatctgaa cgtggcgaag aaaccatcga ggagaagttt gaaatttctg gtaccatgcg tcgcttagct gatcagtctc tgccgccgaa cttttcttgc ctcgaaaact tccgtgctta cgttgatggt ttcgaaccga acggctgtat tgagggcaag ctctcccaga tgagcaagga agtaaacgca cagattgaac cgttcctcaa gaccaccccg cgccctatca agctccctaa cggccctccg tgctaccagc gctctaaatt tctgctgatg gatgcactga aactgtccat cgaagatccg tctcacgagg gtgaaggtat cccgctgtac gacgccatca agtgtattaa aaccttcttc ggttggaagg agccgtatat cgtaaaacca cacgagaagg gcatcaactc taactacctc ctgtcgtgga agcaggttct ctctgaactg caggatatcg aaaacgaaga gaagattcct cgcactaaga atatgaagaa aacttctcag ctgaagtggg cactcggcga gaacatggcc ccagagaagg tggacttcga gaactgccgt gacatctctg acctgaaaca gtatgattcc gacgaaccgg agctgcgttc cctgtcttcc tggattcaga acgaatttaa taaggcttgc gaactgactg actctatttg gattgagctg gacgagatcg gcgaagacgt ggcaccaatc gaatacatag cttccatgcg tcgtaactac ttcaccgcgg aggttagcca ctgtcgcgcc accgaatata tcatgaaagg cgtttatatt aacaccgccc tcctcaacgc atcctgtgca gctatggacg actttcagct cattccgatg atctccaaat gccgcaccaa agagggtcgc cgtaaaacca atttatacgg ttttataatc aagggccgct cacacctgcg caacgacact gacgttgtga acttcgtttc tatggaattt tctctcaccg acccacgtct ggagccacat aaatgggaga aatactgcgt tctggaaata ggcgacatgc tgctgcgttc cgccatcggt cagattagcc gcccaatgtt tctgtatgtt cgtaccaacg gcacttctaa agtgaaaatg aaatggggca tggagatgcg ccgttgcctc ctgcagtctc tccaacaaat cgagagcatg attgaggcag aaagctccgt taaggagaaa gacatgacta aagaattttt cgagaacaaa tctgaagcat ggcctatcgg tgaatcgccg aaaggtgttg aggaaggctc tataggcaag gtttgccgta ccctgttagc taaatctgtt ttcaacagcc tgtacgcaag cccgcaactc gaaggcttct ctgccgaatc tcgcaagctg ctcttagttg ttcaagctct gcgcgataac ttagagcctg gcactttcga cctgggcggc ctgtacgagg caatcgaaga gtgcctcatc aacgatcctt gggtgctgct gaacgcttcc tggtttaaca gcttcctgac ccacgccctg aaataaaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat