InvaA.07057.a

Polymerase acid protein (PA)

CENTER ID:	InvaA.07057.a
ORGANISM:	Influenzavirus A Influenza A virus A/Viet Nam/1203/2004 (H5N1)
ASSOCIATED DISEASE:	Influenza
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
InvaA.07057.a.D13.GE082212a	InvaA.07057.a		257	716
InvaA.07057.a.D14.GE082212a	InvaA.07057.a		2	716

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
InvaA.07057.a.D11.PD00159	InvaA.07057.a		1	716
InvaA.07057.a.D14.PD00165	InvaA.07057.a		2	716

External Resources

RESOURCE	REFERENCE ID
IRD:	link
PATRIC ID:	fig\|284218.13.CDS.2
UniProt:	Q5EP34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEDFVRQCFN PMIVELAEKA MKEYGEDPKI ETNKFAAICT HLEVCFMYSD FHFIDERSES IIVESGDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGV EKPKFLPDLY DYKENRFIEI GVTRREVHTY YLEKANKIKS EETHIHIFSF TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET IEEKFEITGT MRRLADQSLP PNFSSLENFR AYVDGFEPNG CIEGKLSQMS KEVNARIEPF LKTTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MKTFFGWKEP NIVKPHEKGI NPNYLLAWKQ VLAELQDIEN EEKIPKTKNM KKTSQLKWAL GENMAPEKVD FEDCKDVSDL RQYDSDEPES RSLASWIQSE FNKACELTDS IWIELDEIGE DVAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL RTAVGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSETWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIAQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LAHALK

NT Sequence

atggaagact ttgtgcgaca atgcttcaat ccaatgattg tcgagcttgc ggaaaaggca atgaaagaat atggggaaga tccgaaaatc gaaacgaaca agtttgctgc aatatgcaca cacttggagg tctgtttcat gtattcggat tttcacttta ttgatgaacg gagtgaatca ataattgtag aatctggaga tccgaatgca ttattgaaac accgatttga aataattgaa ggaagagacc gaacgatggc ctggactgtg gtgaatagta tctgcaacac cacaggagtt gagaaaccta aatttctccc agatttgtat gactacaaag agaaccgatt catcgaaatt ggagtgacac ggagggaagt tcatacatac tatctggaga aagccaacaa gataaaatcc gaggagacac atattcacat attctcattc acaggggagg aaatggccac caaagcggac tacacccttg atgaagagag cagggcaaga attaaaacca ggctgttcac cataaggcag gaaatggcca gtaggggtct atgggattcc tttcgtcaat ccgagagagg cgaagagaca attgaagaaa aatttgaaat cactggaacc atgcgcagac ttgcagacca aagtctccca ccgaacttct ccagccttga aaactttaga gcctatgtgg atggattcga accgaacggc tgcattgagg gcaagctttc tcaaatgtca aaagaagtga atgctagaat tgagccattt ttgaagacaa cgccacgccc tctcagacta cctgatgggc ctccttgctc tcagcggtcg aagttcttgc tgatggatgc ccttaaatta agcatcgaag acccgagtca tgagggggag gggataccac tatacgatgc aatcaaatgc atgaagacat ttttcggctg gaaagagccc aacatcgtga aaccacatga aaaaggtata aaccccaatt acctcctggc ttggaagcaa gtgctggcag aactccaaga tattgaaaat gaggagaaaa tcccaaaaac aaagaacatg aaaaaaacaa gccagttgaa gtgggcactc ggtgagaaca tggcaccaga gaaagtagac tttgaggact gcaaagatgt tagcgatcta agacagtatg acagtgatga accagagtct agatcactag caagctggat tcagagtgaa ttcaacaagg catgtgaatt gacagattcg atttggattg aactcgatga aataggagaa gacgtagctc caattgagca cattgcaagt atgagaagga actattttac agcggaagta tcccattgca gggccactga atacataatg aagggagtgt acataaacac agccctgttg aatgcatcct gtgcagccat ggatgacttt caactgattc caatgataag caaatgcaga accaaagaag gaagacggaa aactaatctg tatggattca ttataaaagg gagatcccac ttgaggaatg ataccgatgt ggtaaatttt gtgagtatgg aattctctct tactgatccg aggctggagc cacacaagtg ggaaaagtac tgtgtcctcg agataggaga catgctcctc cggactgcag taggccaagt ttcgaggccc atgttcctgt atgtaagaac caatggaacc tccaagatca aaatgaaatg gggcatggaa atgaggcgat gccttcttca atcccttcaa caaattgaaa gcatgattga agccgagtct tctgtcaaag agaaggacat gaccaaagaa ttctttgaaa acaaatcaga aacatggccg attggagagt cccccaaggg agtggaggaa ggctccatcg gaaaggtgtg cagaaccttg ctggcgaagt ctgtgttcaa cagtttatat gcatctccac aactcgaggg gttttcagct gaatcaagaa aattgcttct cattgctcag gcacttaggg acaacctgga acctgggacc ttcgatcttg gagggctata tgaagcaatt gaggagtgcc tgattaacga tccctgggtt ttgcttaatg cgtcttggtt caactccttc ctcgcacatg cactgaaata g

Details for InvaA.07057.a.D13.GE082212a

HARVESTED ON:	11/25/2009
SEQUENCED ON:	9/18/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	45

Validated AA Sequence

MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG IEPFLKTTPR PLRLPDGPPC SQRSKFLLMD ALKLSIEDPS HEGEGIPLYD AIKCMKTFFG WKEPNIVKPH EKGINPNYLL AWKQVLAELQ DIENEEKIPK TKNMKKTSQL KWALGENMAP EKVDFEDCKD VSDLRQYDSD EPESRSLASW IQSEXNKACE LTDSIWIELD EIXEDXAPIE HIASMRRXYF TAEVSHCRAT EXX

Validated NT Sequence

gcgaagtaaa accggaagtg aagccggaga cccacatcaa cctgaaggtt agcgacggta gcagcgaaat cttcttcaag attaagaaga ccaccccgct gcgtcgcctg atggaagcat tcgctaaacg ccagggcaag gaaatggatt ccctgcgctt tctgtacgac ggtatccgta ttcaggcaga ccagactccg gaagatctgg acatggaaga taacgacatt atcgaagcac accgtgaaca aatcggtgga attgaaccgt tcttaaagac tacccctcgc cctctgcgcc tgccagatgg tccgccgtgt tctcagcgtt ccaaattcct gctgatggac gcactgaagc tgtccattga agatcctagc cacgaaggtg aaggtatccc tctgtacgac gcaattaagt gcatgaaaac cttctttggt tggaaggaac ctaacattgt taaacctcac gagaagggca tcaacccaaa ctacctgctg gcatggaaac aggtgctcgc tgaactgcag gacatcgaaa acgaagagaa aattccaaag accaagaata tgaaaaagac ctctcagctg aaatgggcac tgggcgaaaa catggctccn gagaaggtcg attttgagga ctgtaaagac gtttctgatt tgcgtcaata cgactctgac gaaccggaat cccgcagcct cgcatcctgg atccagagcg aanttaacaa agcatgcgaa ctgactgata gcatttggat cgaactggac gaaatcngcg aagacnttgc accgatcgaa cacatagctt cnatgcgccg cnactatttc accgctgaag tttctcattg tcgtgctacc gaanacntca ng

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGIEPF LKTTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MKTFFGWKEP NIVKPHEKGI NPNYLLAWKQ VLAELQDIEN EEKIPKTKNM KKTSQLKWAL GENMAPEKVD FEDCKDVSDL RQYDSDEPES RSLASWIQSE FNKACELTDS IWIELDEIGE DVAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL RTAVGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSETWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIAQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LAHALK

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat tgaaccgttc ttaaagacta cccctcgccc tctgcgcctg ccagatggtc cgccgtgttc tcagcgttcc aaattcctgc tgatggacgc actgaagctg tccattgaag atcctagcca cgaaggtgaa ggtatccctc tgtacgacgc aattaagtgc atgaaaacct tctttggttg gaaggaacct aacattgtta aacctcacga gaagggcatc aacccaaact acctgctggc atggaaacag gtgctcgctg aactgcagga catcgaaaac gaagagaaaa ttccaaagac caagaatatg aaaaagacct ctcagctgaa atgggcactg ggcgaaaaca tggctccgga gaaggtcgat tttgaggact gtaaagacgt ttctgatttg cgtcaatacg actctgacga accggaatcc cgcagcctcg catcctggat ccagagcgaa tttaacaaag catgcgaact gactgatagc atttggatcg aactggacga aatcggcgaa gacgttgcac cgatcgaaca catagcttct atgcgccgca actatttcac cgctgaagtt tctcattgtc gtgctaccga atacatcatg aagggcgttt atatcaacac cgctctcctc aacgcttctt gcgctgctat ggatgacttc caactcattc cgatgatcag caagtgccgc actaaagaag gccgccgcaa gactaatctg tacggtttta tcattaaggg tcgctctcac ctccgcaacg acaccgacgt cgtgaatttc gtgtctatgg agttctccct gaccgacccg cgtctcgagc cgcacaagtg ggagaagtac tgcgtactcg aaatcggcga tatgctgctc cgcaccgcag ttggccaagt ttctcgcccg atgttcctgt acgttcgcac caacggcact tccaaaatca aaatgaagtg gggcatggaa atgcgccgct gcctgctgca atccctccag cagattgagt ctatgatcga agctgaatcc tctgtaaaag aaaaggacat gactaaggaa ttctttgaga acaagtcgga gacctggcct attggcgaat ctcctaaggg cgtcgaagaa ggctccattg gtaaggtgtg tcgtaccctg ttagctaaat ccgtgttcaa ctccctgtac gcttctccac agctggaagg tttctctgcc gaatctcgta aactgctcct gatcgctcag gctctccgtg acaatctgga accgggtacc tttgacttag gcggcctgta cgaagctatc gaggaatgcc tcatcaacga cccgtgggtg ctgctcaacg catcttggtt caactccttt ctggcccacg ctcttaagta aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat

Details for InvaA.07057.a.D14.GE082212a

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/18/2012
EXPECTED MW:	0kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	data unavailable
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	37

Validated AA Sequence

MXRNXFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLXPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL RTAVGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSETWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIAQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV LLNASWFNSF LAHAXXXXXA AALEHXHHXH XXXGXXXXXX X

Validated NT Sequence

cnnnnntncn nnnntnngnn nnggccgnnn tcnnngtgnn ggtggtggng gtgctcgagt gcggccgcan nnntnnnnnn nanagcgtgg gccagaaagg agttgaacca agatgcgttg agcagcaccc acgggtcgtt gatgaggcat tcctcgatag cttcgtacag gccgcctaag tcaaaggtac ccggttccag attgtcacgg agagcctgag cgatcaggag cagtttacga gattcggcag agaaaccttc cagctgtgga gaagcgtaca gggagttgaa cacggattta gctaacaggg tacgacacac cttaccaatg gagccttctt cgacgccctt aggagattcg ccaataggcc aggtctccga cttgttctca aagaattcct tagtcatgtc cttttctttt acagaggatt cagcttcgat catagactca atctgctgga gggattgcag caggcagcgg cgcatttcca tgccccactt cattttgatt ttggaagtgc cgttggtgcg aacgtacagg aacatcgggc gagaaacttg gccaactgcg gtgcggagca gcatatcgcc gatttcgagt acgcagtact tctcccactt gtgcggctcg agacgcgggt cggtcaggga gaactccata gacacgaaat tcacgacgtc ggtgtcgttg cggaggtgag agcgaccctt aatgataaaa ccgtacagat tagtcttgcg gcggccttct ttagtgcggc acttgctgat catcggantg agttggaagt catccatagc agcgcaagaa gcgttgagga gagcggtgtt gatataaacg cccttcatga tgtattcggt agcacgacaa tgagaaactt cagcggtgaa ntagttgcgg ngcatanaan ctatgtgttc natcggngca acgtcttcnc cgatttcntc cagttcnatc cantgctatc agtcagttcg catgctttgt taanttcnct ctggnnccnn nngcgagntg cggnnttncn nncgtcantc gtantgacnc nnncanaaan gtcnttncng tcnnaaanng nccnnnncnt nnttnnnccn nnnncnnntn nanntgnnnn gncntttnnn nngnnnnnnn tnnnntnnnn nnnannnnnn nnnnnnnnnn nncngnncnn ncnnnnnng

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGEDFV RQCFNPMIVE LAEKAMKEYG EDPKIETNKF AAICTHLEVC FMYSDFHFID ERSESIIVES GDPNALLKHR FEIIEGRDRT MAWTVVNSIC NTTGVEKPKF LPDLYDYKEN RFIEIGVTRR EVHTYYLEKA NKIKSEETHI HIFSFTGEEM ATKADYTLDE ESRARIKTRL FTIRQEMASR GLWDSFRQSE RGEETIEEKF EITGTMRRLA DQSLPPNFSS LENFRAYVDG FEPNGCIEGK LSQMSKEVNA RIEPFLKTTP RPLRLPDGPP CSQRSKFLLM DALKLSIEDP SHEGEGIPLY DAIKCMKTFF GWKEPNIVKP HEKGINPNYL LAWKQVLAEL QDIENEEKIP KTKNMKKTSQ LKWALGENMA PEKVDFEDCK DVSDLRQYDS DEPESRSLAS WIQSEFNKAC ELTDSIWIEL DEIGEDVAPI EHIASMRRNY FTAEVSHCRA TEYIMKGVYI NTALLNASCA AMDDFQLIPM ISKCRTKEGR RKTNLYGFII KGRSHLRNDT DVVNFVSMEF SLTDPRLEPH KWEKYCVLEI GDMLLRTAVG QVSRPMFLYV RTNGTSKIKM KWGMEMRRCL LQSLQQIESM IEAESSVKEK DMTKEFFENK SETWPIGESP KGVEEGSIGK VCRTLLAKSV FNSLYASPQL EGFSAESRKL LLIAQALRDN LEPGTFDLGG LYEAIEECLI NDPWVLLNAS WFNSFLAHAL K

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaga agacttcgtt cgtcagtgct ttaacccaat gatcgttgag ctggccgaaa aagccatgaa ggagtacggc gaagacccta agatcgaaac caacaagttt gcagccatct gtactcacct tgaagtgtgc tttatgtaca gcgattttca cttcattgat gaacgcagcg agtctataat cgttgaatct ggtgacccga acgccctcct gaagcaccgt ttcgaaatca tcgagggtcg cgatcgtact atggcttgga ctgtagttaa ctccatctgc aacactactg gcgtggagaa gccaaagttc ctccctgatc tgtatgacta caaagaaaac cgctttatcg aaatcggtgt gacccgccgc gaagttcaca cctactatct ggagaaggcc aataaaatca agtctgaaga gacccatatc catatcttct ccttcaccgg cgaagaaatg gctactaaag ctgactacac tctggatgag gaatctcgcg ctcgcattaa aacacgtctc ttcaccattc gtcaggaaat ggcatcccgc ggtctgtggg attccttccg tcagtctgag cgcggcgagg aaactatcga ggaaaagttt gaaataaccg gcaccatgcg tcgtttagct gatcaaagcc tgccgccgaa cttttcttcc ctcgaaaact tccgtgcata cgtggacggt ttcgagccga acggttgcat tgaaggcaaa ctgagccaga tgagcaaaga agtcaacgcc cgtattgaac cgttcttaaa gactacccct cgccctctgc gcctgccaga tggtccgccg tgttctcagc gttccaaatt cctgctgatg gacgcactga agctgtccat tgaagatcct agccacgaag gtgaaggtat ccctctgtac gacgcaatta agtgcatgaa aaccttcttt ggttggaagg aacctaacat tgttaaacct cacgagaagg gcatcaaccc aaactacctg ctggcatgga aacaggtgct cgctgaactg caggacatcg aaaacgaaga gaaaattcca aagaccaaga atatgaaaaa gacctctcag ctgaaatggg cactgggcga aaacatggct ccggagaagg tcgattttga ggactgtaaa gacgtttctg atttgcgtca atacgactct gacgaaccgg aatcccgcag cctcgcatcc tggatccaga gcgaatttaa caaagcatgc gaactgactg atagcatttg gatcgaactg gacgaaatcg gcgaagacgt tgcaccgatc gaacacatag cttctatgcg ccgcaactat ttcaccgctg aagtttctca ttgtcgtgct accgaataca tcatgaaggg cgtttatatc aacaccgctc tcctcaacgc ttcttgcgct gctatggatg acttccaact cattccgatg atcagcaagt gccgcactaa agaaggccgc cgcaagacta atctgtacgg ttttatcatt aagggtcgct ctcacctccg caacgacacc gacgtcgtga atttcgtgtc tatggagttc tccctgaccg acccgcgtct cgagccgcac aagtgggaga agtactgcgt actcgaaatc ggcgatatgc tgctccgcac cgcagttggc caagtttctc gcccgatgtt cctgtacgtt cgcaccaacg gcacttccaa aatcaaaatg aagtggggca tggaaatgcg ccgctgcctg ctgcaatccc tccagcagat tgagtctatg atcgaagctg aatcctctgt aaaagaaaag gacatgacta aggaattctt tgagaacaag tcggagacct ggcctattgg cgaatctcct aagggcgtcg aagaaggctc cattggtaag gtgtgtcgta ccctgttagc taaatccgtg ttcaactccc tgtacgcttc tccacagctg gaaggtttct ctgccgaatc tcgtaaactg ctcctgatcg ctcaggctct ccgtgacaat ctggaaccgg gtacctttga cttaggcggc ctgtacgaag ctatcgagga atgcctcatc aacgacccgt gggtgctgct caacgcatct tggttcaact cctttctggc ccacgctctt aagtaaaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat

Details for InvaA.07057.a.D11.PD00159

PURIFICATION DATe:	8/27/2010
CONCENTRATION:	18.75mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	38

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat ggaagacttc gttcgtcagt gctttaaccc aatgatcgtt gagctggccg aaaaagccat gaaggagtac ggcgaagacc ctaagatcga aaccaacaag tttgcagcca tctgtactca ccttgaagtg tgctttatgt acagcgattt tcacttcatt gatgaacgca gcgagtctat aatcgttgaa tctggtgacc cgaacgccct cctgaagcac cgtttcgaaa tcatcgaggg tcgcgatcgt actatggctt ggactgtagt taactccatc tgcaacacta ctggcgtgga gaagccaaag ttcctccctg atctgtatga ctacaaagaa aaccgcttta tcgaaatcgg tgtgacccgc cgcgaagttc acacctacta tctggagaag gncaataaaa tcaagtctga agagacccat atccatatct tctccttcac cggcgaagaa atggctacta aagctgacta cactctggat gaggaatctc gcgctcgcat taaaacacgt ctcttcacca ttcgtcagga aatggcatcc cgcggtctgt gggattcctt ccgtcagtct gagcgcggcg aggaaactat cgaggaaaag tttgaaatna ccngnaccat gcgtcgttta gctgatcaaa gcctg

Expressed Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMEDF VRQCFNPMIV ELAEKAMKEY GEDPKIETNK FAAICTHLEV CFMYSDFHFI DERSESIIVE SGDPNALLKH RFEIIEGRDR TMAWTVVNSI CNTTGVEKPK FLPDLYDYKE NRFIEIGVTR REVHTYYLEK ANKIKSEETH IHIFSFTGEE MATKADYTLD EESRARIKTR LFTIRQEMAS RGLWDSFRQS ERGEETIEEK FEITGTMRRL ADQSLPPNFS SLENFRAYVD GFEPNGCIEG KLSQMSKEVN ARIEPFLKTT PRPLRLPDGP PCSQRSKFLL MDALKLSIED PSHEGEGIPL YDAIKCMKTF FGWKEPNIVK PHEKGINPNY LLAWKQVLAE LQDIENEEKI PKTKNMKKTS QLKWALGENM APEKVDFEDC KDVSDLRQYD SDEPESRSLA SWIQSEFNKA CELTDSIWIE LDEIGEDVAP IEHIASMRRN YFTAEVSHCR ATEYIMKGVY INTALLNASC AAMDDFQLIP MISKCRTKEG RRKTNLYGFI IKGRSHLRND TDVVNFVSME FSLTDPRLEP HKWEKYCVLE IGDMLLRTAV GQVSRPMFLY VRTNGTSKIK MKWGMEMRRC LLQSLQQIES MIEAESSVKE KDMTKEFFEN KSETWPIGES PKGVEEGSIG KVCRTLLAKS VFNSLYASPQ LEGFSAESRK LLLIAQALRD NLEPGTFDLG GLYEAIEECL INDPWVLLNA SWFNSFLAHA LK

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GGAAGACTTC GTTCGTCAGT GCTTTAACCC AATGATCGTT GAGCTGGCCG AAAAAGCCAT GAAGGAGTAC GGCGAAGACC CTAAGATCGA AACCAACAAG TTTGCAGCCA TCTGTACTCA CCTTGAAGTG TGCTTTATGT ACAGCGATTT TCACTTCATT GATGAACGCA GCGAGTCTAT AATCGTTGAA TCTGGTGACC CGAACGCCCT CCTGAAGCAC CGTTTCGAAA TCATCGAGGG TCGCGATCGT ACTATGGCTT GGACTGTAGT TAACTCCATC TGCAACACTA CTGGCGTGGA GAAGCCAAAG TTCCTCCCTG ATCTGTATGA CTACAAAGAA AACCGCTTTA TCGAAATCGG TGTGACCCGC CGCGAAGTTC ACACCTACTA TCTGGAGAAG GCCAATAAAA TCAAGTCTGA AGAGACCCAT ATCCATATCT TCTCCTTCAC CGGCGAAGAA ATGGCTACTA AAGCTGACTA CACTCTGGAT GAGGAATCTC GCGCTCGCAT TAAAACACGT CTCTTCACCA TTCGTCAGGA AATGGCATCC CGCGGTCTGT GGGATTCCTT CCGTCAGTCT GAGCGCGGCG AGGAAACTAT CGAGGAAAAG TTTGAAATAA CCGGCACCAT GCGTCGTTTA GCTGATCAAA GCCTGCCGCC GAACTTTTCT TCCCTCGAAA ACTTCCGTGC ATACGTGGAC GGTTTCGAGC CGAACGGTTG CATTGAAGGC AAACTGAGCC AGATGAGCAA AGAAGTCAAC GCCCGTATTG AACCGTTCTT AAAGACTACC CCTCGCCCTC TGCGCCTGCC AGATGGTCCG CCGTGTTCTC AGCGTTCCAA ATTCCTGCTG ATGGACGCAC TGAAGCTGTC CATTGAAGAT CCTAGCCACG AAGGTGAAGG TATCCCTCTG TACGACGCAA TTAAGTGCAT GAAAACCTTC TTTGGTTGGA AGGAACCTAA CATTGTTAAA CCTCACGAGA AGGGCATCAA CCCAAACTAC CTGCTGGCAT GGAAACAGGT GCTCGCTGAA CTGCAGGACA TCGAAAACGA AGAGAAAATT CCAAAGACCA AGAATATGAA AAAGACCTCT CAGCTGAAAT GGGCACTGGG CGAAAACATG GCTCCGGAGA AGGTCGATTT TGAGGACTGT AAAGACGTTT CTGATTTGCG TCAATACGAC TCTGACGAAC CGGAATCCCG CAGCCTCGCA TCCTGGATCC AGAGCGAATT TAACAAAGCA TGCGAACTGA CTGATAGCAT TTGGATCGAA CTGGACGAAA TCGGCGAAGA CGTTGCACCG ATCGAACACA TAGCTTCTAT GCGCCGCAAC TATTTCACCG CTGAAGTTTC TCATTGTCGT GCTACCGAAT ACATCATGAA GGGCGTTTAT ATCAACACCG CTCTCCTCAA CGCTTCTTGC GCTGCTATGG ATGACTTCCA ACTCATTCCG ATGATCAGCA AGTGCCGCAC TAAAGAAGGC CGCCGCAAGA CTAATCTGTA CGGTTTTATC ATTAAGGGTC GCTCTCACCT CCGCAACGAC ACCGACGTCG TGAATTTCGT GTCTATGGAG TTCTCCCTGA CCGACCCGCG TCTCGAGCCG CACAAGTGGG AGAAGTACTG CGTACTCGAA ATCGGCGATA TGCTGCTCCG CACCGCAGTT GGCCAAGTTT CTCGCCCGAT GTTCCTGTAC GTTCGCACCA ACGGCACTTC CAAAATCAAA ATGAAGTGGG GCATGGAAAT GCGCCGCTGC CTGCTGCAAT CCCTCCAGCA GATTGAGTCT ATGATCGAAG CTGAATCCTC TGTAAAAGAA AAGGACATGA CTAAGGAATT CTTTGAGAAC AAGTCGGAGA CCTGGCCTAT TGGCGAATCT CCTAAGGGCG TCGAAGAAGG CTCCATTGGT AAGGTGTGTC GTACCCTGTT AGCTAAATCC GTGTTCAACT CCCTGTACGC TTCTCCACAG CTGGAAGGTT TCTCTGCCGA ATCTCGTAAA CTGCTCCTGA TCGCTCAGGC TCTCCGTGAC AATCTGGAAC CGGGTACCTT TGACTTAGGC GGCCTGTACG AAGCTATCGA GGAATGCCTC ATCAACGACC CGTGGGTGCT GCTCAACGCA TCTTGGTTCA ACTCCTTTCT GGCCCACGCT CTTAAGTAAA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT

Details for InvaA.07057.a.D14.PD00165

PURIFICATION DATe:	9/10/2010
CONCENTRATION:	20.31mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	37

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAGA AGACTTCGTT CGTCAGTGCT TTAACCCAAT GATCGTTGAG CTGGCCGAAA AAGCCATGAA GGAGTACGGC GAAGACCCTA AGATCGAAAC CAACAAGTTT GCAGCCATCT GTACTCACCT TGAAGTGTGC TTTATGTACA GCGATTTTCA CTTCATTGAT GAACGCAGCG AGTCTATAAT CGTTGAATCT GGTGACCCGA ACGCCCTCCT GAAGCACCGT TTCGAAATCA TCGAGGGTCG CGATCGTACT ATGGCTTGGA CTGTAGTTAA CTCCATCTGC AACACTACTG GCGTGGAGAA GCCAAAGTTC CTCCCTGATC TGTATGACTA CAAAGAAAAC CGCTTTATCG AAATCGGTGT GACCCGCCGC GAAGTTCACA CCTACTATCT GGAGAAGGCC AATAAAATCA AGTCTGAAGA GACCCATATC CATATCTTCT CCTTCACCGG CGAAGAAATG GCTACTAAAG CTGACTACAC TCTGGATGAG GAATCTCGCG CTCGCATTAA AACACGTCTC TTCACCATTC GTCAGGAAAT GGCATCCCGC GGTCTGTGGG ATTCCTTCCG TCAGTCTGAG CGCGGCGAGG AAACTATCGA GGAAAAGTTT GAAATAACCG GCACCATGCG TCGTTTAGCT GATCAAAGCC TGCCGCCGAA CTTTTCTTCC CTCGAAAACT TCCGTGCATA CGTGGACGGT TTCGAGCCGA ACGGTTGCAT TGAAGGCAAA CTGAGCCAGA TGAGCAAAGA AGTCAACGCC CGTATTGAAC CGTTCTTAAA GACTACCCCT CGCCCTCTGC GCCTGCCAGA TGGTCCGCCG TGTTCTCAGC GTTCCAAATT CCTGCTGATG GACGCACTGA AGCTGTCCAT TGAAGATCCT AGCCACGAAG GTGAAGGTAT CCCTCTGTAC GACGCAATTA AGTGCATGAA AACCTTCTTT GGTTGGAAGG AACCTAACAT TGTTAAACCT CACGAGAAGG GCATCAACCC AAACTACCTG CTGGCATGGA AACAGGTGCT CGCTGAACTG CAGGACATCG AAAACGAAGA GAAAATTCCA AAGACCAAGA ATATGAAAAA GACCTCTCAG CTGAAATGGG CACTGGGCGA AAACATGGCT CCGGAGAAGG TCGATTTTGA GGACTGTAAA GACGTTTCTG ATTTGCGTCA ATACGACTCT GACGAACCGG AATCCCGCAG CCTCGCATCC TGGATCCAGA GCGAATTTAA CAAAGCATGC GAACTGACTG ATAGCATTTG GATCGAACTG GACGAAATCG GCGAAGACGT TGCACCGATC GAACACATAG CTTCTATGCG CCGCAACTAT TTCACCGCTG AAGTTTCTCA TTGTCGTGCT ACCGAATACA TCATGAAGGG CGTTTATATC AACACCGCTC TCCTCAACGC TTCTTGCGCT GCTATGGATG ACTTCCAACT CATTCCGATG ATCAGCAAGT GCCGCACTAA AGAAGGCCGC CGCAAGACTA ATCTGTACGG TTTTATCATT AAGGGTCGCT CTCACCTCCG CAACGACACC GACGTCGTGA ATTTCGTGTC TATGGAGTTC TCCCTGACCG ACCCGCGTCT CGAGCCGCAC AAGTGGGAGA AGTACTGCGT ACTCGAAATC GGCGATATGC TGCTCCGCAC CGCAGTTGGC CAAGTTTCTC GCCCGATGTT CCTGTACGTT CGCACCAACG GCACTTCCAA AATCAAAATG AAGTGGGGCA TGGAAATGCG CCGCTGCCTG CTGCAATCCC TCCAGCAGAT TGAGTCTATG ATCGAAGCTG AATCCTCTGT AAAAGAAAAG GACATGACTA AGGAATTCTT TGAGAACAAG TCGGAGACCT GGCCTATTGG CGAATCTCCT AAGGGCGTCG AAGAAGGCTC CATTGGTAAG GTGTGTCGTA CCCTGTTAGC TAAATCCGTG TTCAACTCCC TGTACGCTTC TCCACAGCTG GAAGGTTTCT CTGCCGAATC TCGTAAACTG CTCCTGATCG CTCAGGCTCT CCGTGACAAT CTGGAACCGG GTACCTTTGA CTTAGGCGGC CTGTACGAAG CTATCGAGGA ATGCCTCATC AACGACCCGT GGGTGCTGCT CAACGCATCT TGGTTCAACT CCTTTCTGGC CCACGCTCTT AAGTAAAAGC TTGCGGCCGC ACTCGAGCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGAT