HevcB.17238.b

NS5A

CENTER ID: HevcB.17238.b
ORGANISM: Hepacivirus Hepatitis C virus
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HevcB.17238.b.A11.GE31364 full length 1 447
HevcB.17238.b.A12.GE31365 full length 1 447
HevcB.17238.b.A14.GE31367 full length 1 447
HevcB.17238.b.A15.GE31368 full length 1 447
HevcB.17238.b.A16.GE31369 full length 1 447
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
UniProt: Q0MR50
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
SGSWLRDVWD WICMVLTDFK TWLQSKLLPR LPGVPFFSCQ RGYKGVWRGD GIMQTTCPCG ARITGHVKNG PMRIVGPKTC SNTWHGTFPI NAYTTGPCTP SPAPNYSRAL WRVAAEEYVE VTRVGDFHYV TGMTTDNVKC PCQVPAPEFF TEVDGVRLHR YAPACKPLLR DEVTFQVGLN QYLVGSQLPC EPEPDVAVLT SMLTDPSHIT AETAKRRLAR GSPPSLASSS ASQLSAPSLK ATCTTNHDSP GADLIEANLL WRQEMGGNIT RVESENKVVI LDSFDPLRAE EDEKELSVEA EILRKSRKFP SALPIWARPD YNPPLIESWK DPDYAPPVVH GCPLPPTKAP PIPPPRRKRT VILTDSTVSS ALAELATKTF GSSGSSVVDS GTATGPPDQA SGDGDEGSDA ESYSSMPPLE GEPGDPDLSD GSWSTVSEEA SEDVVCC
NT Sequence
tccggttcgt ggctaaggga tgtttgggac tggatatgca tggtgttgac tgatttcaag acctggctcc agtccaagct cctgccgcgg ttaccaggag tccccttctt ctcatgccag cgtgggtata agggagtctg gcgaggggac ggcatcatgc aaaccacctg cccatgtgga gcacggatca caggccatgt caagaacggt cccatgagga tcgtcgggcc taagacctgc agcaacacgt ggcatggaac atttcccatc aacgcataca ccacgggccc ttgcacgccc tccccggcgc caaactattc tagggcgcta tggcgggtgg ctgctgagga gtatgtggag gttacgcggg tgggggattt ccactacgtg acgggcatga ccactgacaa cgtaaagtgc ccatgccagg ttccggctcc cgaattcttc acggaggtgg atggagtacg gttgcacagg tacgctccgg cgtgcaaacc tctcctacgg gatgaggtca cattccaggt cgggcttaac cagtacctgg ttgggtcgca gcttccatgc gagcccgaac cggacgtagc agtgcttact tccatgctca ccgacccctc ccacattaca gcagagacgg ctaagcgtag actggccagg gggtctcccc cctccttggc cagctcgtca gctagccagt tgtctgcgcc ttccttgaag gcgacatgca ctaccaatca tgactccccg ggcgctgacc tcatcgaggc taacctcctg tggcggcagg agatgggcgg gaacatcacc cgcgtggagt cggagaataa ggtggtgatt ctggactctt tcgacccgct tcgagcggag gaggacgaga aggaattgtc cgtcgaggcg gaaatcctgc ggaaatccag gaagttcccc tcagcgctgc ctatatgggc acggccggac tacaaccctc cattgataga gtcctggaaa gacccggact acgcccctcc ggtggtacac gggtgcccat tgccacctac caaggctccc ccaataccac ccccacggag aaagaggacg gttatcctga cagattccac tgtgtcttct gccttggcgg agctcgctac taagaccttc ggcagctccg gatcgtcagt cgtcgacagc ggcacggcga ccggccctcc tgaccaggct tccggcgacg gcgacgaagg atccgacgct gagtcgtact cctccatgcc cccccttgag ggggagccgg gggaccccga tctcagcgac gggtcttggt ctaccgtgag cgaggaggct agtgaggacg tcgtctgctg c
Details for HevcB.17238.b.A11.GE31364
HARVESTED ON: 2/8/2010
SEQUENCED ON: 2/14/2011
EXPECTED MW: 51kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 42
PERCENT COVERAGE: 71
Validated AA Sequence
MFXXXXXXXX XXXXXXXXXX TGPGSMSGXW LRDVWDWICT VLTDFKTWLQ SKLLPRLPGV PFFSCQRGYK GVWRGDGIMQ TTCPCGAQIT GHVKNGSMRI VGPRTCXNTW HGTFPINAYT TGPCTPSPXP NYXRALWRVA AXXYVEVTXX XDFHXXXGXX XDXXKXPXXX PXXXSSPXXT AFVCXXXHXX VNXCXXXXXL SXXXXXXSXX XXXXXXXXXX LXXXLXXXLX XXXXXXRXXX XXXXXXXXXP XXXXXXNXXX XXSEXXXXXX PXXXXPDVXX XXXXXXXXXX XXXXXXXXXX XXXXXXLXPX XXXXXXXXXX XXXXXXXPXX XXXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn gcggnncagc catgtttncn tnacnccnnn nannnnnnan naannannnn nngnnnncnn ngnnnnntca naccggtcct ggttcgatgt ctggtanctg gctgcgcgat gtctgggact ggatctgcac cgtgctgacc gacttcaaga cttggctgca gtctaaactc ctgcctcgcc tccctggcgt tcctttcttt agctgccagc gtggctataa aggcgtatgg cgtggtgacg gtataatgca gactacttgc ccatgcggtg cacagattac cggtcacgtg aagaatggta gcatgcgtat agttggtccg cgtacttgcn gcaacacttg gcacggcact ttccctatca acgcctacac cactggccct tgtactcctt ctccngntcc gaactacanc cgtgcactct ggcgngtagc tgcnnaanag tacgtggagg ttacccntgn cngngatttc cattncnnnn ccggnnnnnn nnccgatnac gntaaangtc cnngcnnngn tccgnncnnn nnntcttcac cgaannnnac ngcgttcgtc tgctnnnntn cgcaccnnnc ngtnaacnnc tgctgnnnna nnannnnnct ttcttanntg ngtncnanna nntcnngngn nnnnnnntgn ngngnnnnnn agnnnnnnnc nttgnnnnnn nnactancnn gctgnctgat nnnncnnana nnanngnaca gacngntann nnncgncnnn nnnnngntnn nccnncntcc cnngnatnnn nngnnagnca acnnagntgn nncntnntct gaaangaann tgnnnngtnn nccnnnannn nnngancctg atgtgatnnn nnnnnaccnn nntngcnnnn nnnnnnnnnn nnacntannn nnnnnnnngn nnganannan gnaannatan ntgnnnnnnc tncnnccnnn annnnnnnna naanaannan nnnnnnnncn tnnnnnnnnn cnnnnnnnnn nncccnnnan nnnnaannan nncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGSWLRDV WDWICTVLTD FKTWLQSKLL PRLPGVPFFS CQRGYKGVWR GDGIMQTTCP CGAQITGHVK NGSMRIVGPR TCSNTWHGTF PINAYTTGPC TPSPAPNYSR ALWRVAAEEY VEVTRVGDFH YVTGMTTDNV KCPCQVPAPE FFTEVDGVRL HRYAPACKPL LREEVTFLVG LNQYLVGSQL PCEPEPDVAV LTSMLTDPSH ITAETAKRRL ARGSPPSLAS SSASQLSAPS LKATCTTRHD SPDADLIEAN LLWRQEMGGN ITRVESENKV VILDSFEPLQ AEEDEREVSV PAEILRRSRK FPRAMPIWAR PDYNPPLLES WKDPDYVPPV VHGCPLPPAK APPIPPPRRK RTVVLSESTV SSALAELATK TFGSSESSAV DSGTATASPD QPSDDGDAGS DVESYSSMPP LEGEPGDPDL SDGSWSTVSE EASEDVVCC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggtagc tggctgcgcg atgtctggga ctggatctgc accgtgctga ccgacttcaa gacttggctg cagtctaaac tcctgcctcg cctccctggc gttcctttct ttagctgcca gcgtggctat aaaggcgtat ggcgtggtga cggtataatg cagactactt gcccatgcgg tgcacagatt accggtcacg tgaagaatgg tagcatgcgt atagttggtc cgcgtacttg cagcaacact tggcacggca ctttccctat caacgcctac accactggcc cttgtactcc ttctccggct ccgaactaca gccgtgcact ctggcgtgta gctgcagaag agtacgtgga ggttacccgt gtcggtgatt tccattacgt gaccggtatg accaccgata acgttaaatg tccgtgccag gttcctgcac cagaattctt caccgaagtc gacggcgttc gtctgcaccg ctacgcaccg gcctgtaaac cgctgctgcg tgaagaggta actttcttag ttggcctgaa ccagtacctc gtgggttctc agctgccgtg cgaaccagaa cctgacgttg cagttcttac tagcatgctg actgatcctt ctcacataac cgcagagact gctaaacgtc gtctcgctcg tggttctccg ccgtccctgg catcttcctc tgctagccaa ctgagcgcac cttctctgaa agcaacctgc actacgcgcc acgatagccc tgacgctgat ctgattgaag ctaacctgct gtggcgccag gaaatgggcg gtaacataac ccgcgttgag agcgagaata aagtagttat tcttgactcc ttcgagccgc tgcaagcaga agaagacgaa cgtgaagttt ctgtgcctgc tgaaatcctg cgtcgttccc gtaaattccc tcgtgccatg ccgatctggg cacgccctga ctacaacccg ccgctgctgg aatcctggaa agatcctgat tacgttccgc ctgtagttca cggctgtcct ctgccacctg ctaaagctcc accgatccca ccgcctcgtc gcaagcgtac cgttgtgttg tccgaatcca ctgtttctag cgccttagct gaactggcaa ctaagacctt cggtagctcc gagtcctccg cagttgattc tggtaccgct accgcctctc ctgaccagcc ttccgatgat ggtgatgccg gtagcgacgt agagtcctac agcagcatgc cgcctctcga aggtgaaccg ggtgatcctg acctgagcga tggttcttgg agcactgtta gcgaagaagc ctcagaagac gtagtttgct gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for HevcB.17238.b.A12.GE31365
HARVESTED ON: data unavailable
SEQUENCED ON: 2/14/2011
EXPECTED MW: 24kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 43
PERCENT COVERAGE: 89
Validated AA Sequence
MFXXLXXIXX XXXXXXXXXX XXGPGSMSGS WLRDVWDWIC TVLTDFKTWL QSKLLPRLPG VPFFSCQRGY XGVWRGDGIX XTTCPCGAQI TGHVKNXXXX IXGXXXCSXX XXXLXXXTXX XXGXXXXXXX PNXXRXXXXX XXXXXXXVXX XXXXXXXXXT XXXXXXSXXL XXXXXPXXRX XLXXLXXXGX XXXXXX
Validated NT Sequence
gcnnnncagc catgtttnnn tnactcntnn nnatccnnnn nnnnnganna nnnnnnnnna nnannaannc aannnnnggt cctggttcga tgtctggtag ctggctgcgc gatgtctggg actggatctg caccgtgctg accgacttca agacttggct gcagtctaaa ctcctgcctc gcctccctgg cgttcctttc tttagctgcc agcgtggcta tnanggcgta tggcgtggtg acggnatant gnanactact tgcccatgcg gtgcacagat taccggtcac gtgaagaatg nnancangcn natanntggn ncnnntnctt gcagcnnnnt tgnnnnnnna ctttnnntnt naacgncnna anccantggc nntngtnnnn nntnnncngn tccnaactnn nnncgnnnnn nnnnngngnn nnnncnnnnn annannnnnn nngttannnn gnnnnnnnnn nngtgnancn ggnagnncac cnnnannnnn nntngncnnt cgnnggntct nnnccnnnnn nnnnncccna nnnntcgnnn tnnnctnnnn nngctacnnn ncncgggnna ccnnnnncnn nnannnnnga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGSWLRDV WDWICTVLTD FKTWLQSKLL PRLPGVPFFS CQRGYKGVWR GDGIMQTTCP CGAQITGHVK NGSMRIVGPR TCSNTWHGTF PINAYTTGPC TPSPAPNYSR ALWRVAAEEY VEVTRVGDFH YVTGMTTDNV KCPCQVPAPE FFTEVDGVRL HRYAPACKPL LREEVTFLVG LNQYLVGSQL PCEPEPDVAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggtagc tggctgcgcg atgtctggga ctggatctgc accgtgctga ccgacttcaa gacttggctg cagtctaaac tcctgcctcg cctccctggc gttcctttct ttagctgcca gcgtggctat aaaggcgtat ggcgtggtga cggtataatg cagactactt gcccatgcgg tgcacagatt accggtcacg tgaagaatgg tagcatgcgt atagttggtc cgcgtacttg cagcaacact tggcacggca ctttccctat caacgcctac accactggcc cttgtactcc ttctccggct ccgaactaca gccgtgcact ctggcgtgta gctgcagaag agtacgtgga ggttacccgt gtcggtgatt tccattacgt gaccggtatg accaccgata acgttaaatg tccgtgccag gttcctgcac cagaattctt caccgaagtc gacggcgttc gtctgcaccg ctacgcaccg gcctgtaaac cgctgctgcg tgaagaggta actttcttag ttggcctgaa ccagtacctc gtgggttctc agctgccgtg cgaaccagaa cctgacgttg cagtttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for HevcB.17238.b.A14.GE31367
HARVESTED ON: data unavailable
SEQUENCED ON: 2/14/2011
EXPECTED MW: 20kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPGVPFFS CQRGYKGVWR GDGIMQTTCP CGAQITGHVK NGSMRIVGPR TCSNTWHGTF PINAYTTGPC TPSPAPNYSR ALWRVAAEEY VEVTRVGDFH YVTGMTTDNV KCPCQVPAPE FFTEVDGVRL HRYAPACKPL LREEVTFLVG LNQYLVGSQL PCEPEPDVAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctccctggc gttcctttct ttagctgcca gcgtggctat aaaggcgtat ggcgtggtga cggtataatg cagactactt gcccatgcgg tgcacagatt accggtcacg tgaagaatgg tagcatgcgt atagttggtc cgcgtacttg cagcaacact tggcacggca ctttccctat caacgcctac accactggcc cttgtactcc ttctccggct ccgaactaca gccgtgcact ctggcgtgta gctgcagaag agtacgtgga ggttacccgt gtcggtgatt tccattacgt gaccggtatg accaccgata acgttaaatg tccgtgccag gttcctgcac cagaattctt caccgaagtc gacggcgttc gtctgcaccg ctacgcaccg gcctgtaaac cgctgctgcg tgaagaggta actttcttag ttggcctgaa ccagtacctc gtgggttctc agctgccgtg cgaaccagaa cctgacgttg cagtttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for HevcB.17238.b.A15.GE31368
HARVESTED ON: 2/8/2010
SEQUENCED ON: 2/14/2011
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 67
Validated AA Sequence
MFXXTPXXXX XXXXXXGXXX AQTQGPGSMF FSCQRGYKGV WRGDGIMQTT CPCGAQITGH VKNGSMRIVG PRTCSNTWHG TFPINAYTTG PCTPSPAPNY SRALWRVAAE EYVEVTRVGD FHYVTGMTTD NVKCPCQVPA PEFFTEVDGV RLHRYAPACK PLLREEVTFL VGLNXYLVGS QLPXEPXPDV AVLTSMXTDP SHITAETAXX XLARGXXXXL ASSSXXXXSA PSLXXXXTTX XDXXDAXLXX ANLLWXXEXX GXITXXESEX KVXXLDSXXX LQAXEXXXXX XXXX
Validated NT Sequence
gnncagccat gtttannnnn actcctntng nnnnnnnnnn nnnnnannat cannngggtn cnnngnnagc tcagacccag ggtcctggtt cgatgttctt tagctgccag cgtggctata aaggcgtatg gcgtggtgac ggtataatgc agactacttg cccatgcggt gcacagatta ccggtcacgt gaagaatggt agcatgcgta tagttggtcc gcgtacttgc agcaacactt ggcacggcac tttccctatc aacgcctaca ccactggccc ttgtactcct tctccggctc cgaactacag ccgtgcactc tggcgtgtag ctgcagaaga gtacgtggag gttacccgtg tcggtgattt ccattacgtg accggtatga ccaccgataa cgttaaatgt ccgtgccagg ttcctgcacc agaattcttc accgaagtcg acggcgttcg tctgcaccgc tacgcaccgg cctgtaaacc gctgctgcgt gaagaggtaa ctttcttagt tggnctgaac cantacctcg tgggntctca gctgccnngc gaaccanaac ctgacgttgc agttcttact agcatgntga ctgatccttc tcacataacc gcagagactg ctaancntcn nctcgctcgt ggnnctncnn cgncnctggc atcttcntct gntannnnnn tgagcgcacc ttctctgann gnaannngna ctacgngcnn cgatagnnct gacgctganc tgantgnagc taacctgctg tggngccnng aaanggncgg tnncataacn ngcgnngaga gcgagnataa agtanntntt cttgactccn tnnagncnct gcaagcanan gaanannnan natnnnnttn nnntncntnn tgaannnctg cgtcgttccc gtnaattcct tcnngnnann nnnannnggg cannncnnnn nnctacaann nnnngnngct ggnnnnnnng nannnannct tnnnnnnnnt ncngnnngnn nnttnnnngn tgnnnnnnnn nnnnnngnnn naannnnnan nnnanncnnn ncnnnnntnn ncnnnnnnnn annnnnnnnn nnnnnnnnnn ntnnnnnnnn nntncnnnnn nnnnnnnnnn ncnnnnnnnn tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccnn nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFFSCQRGY KGVWRGDGIM QTTCPCGAQI TGHVKNGSMR IVGPRTCSNT WHGTFPINAY TTGPCTPSPA PNYSRALWRV AAEEYVEVTR VGDFHYVTGM TTDNVKCPCQ VPAPEFFTEV DGVRLHRYAP ACKPLLREEV TFLVGLNQYL VGSQLPCEPE PDVAVLTSML TDPSHITAET AKRRLARGSP PSLASSSASQ LSAPSLKATC TTRHDSPDAD LIEANLLWRQ EMGGNITRVE SENKVVILDS FEPLQAEEDE REVSVPAEIL RRSRKFPRAM PIWARPDYNP PLLESWKDPD YVPPVVHGCP LPPAKAPPIP PPRRKRTVVL SESTVSSALA ELATKTFGSS ESSAVDSGTA TASPDQPSDD GDAGSDVESY SSMPPLEGEP GDPDLSDGSW STVSEEASED VVCC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttctttagc tgccagcgtg gctataaagg cgtatggcgt ggtgacggta taatgcagac tacttgccca tgcggtgcac agattaccgg tcacgtgaag aatggtagca tgcgtatagt tggtccgcgt acttgcagca acacttggca cggcactttc cctatcaacg cctacaccac tggcccttgt actccttctc cggctccgaa ctacagccgt gcactctggc gtgtagctgc agaagagtac gtggaggtta cccgtgtcgg tgatttccat tacgtgaccg gtatgaccac cgataacgtt aaatgtccgt gccaggttcc tgcaccagaa ttcttcaccg aagtcgacgg cgttcgtctg caccgctacg caccggcctg taaaccgctg ctgcgtgaag aggtaacttt cttagttggc ctgaaccagt acctcgtggg ttctcagctg ccgtgcgaac cagaacctga cgttgcagtt cttactagca tgctgactga tccttctcac ataaccgcag agactgctaa acgtcgtctc gctcgtggtt ctccgccgtc cctggcatct tcctctgcta gccaactgag cgcaccttct ctgaaagcaa cctgcactac gcgccacgat agccctgacg ctgatctgat tgaagctaac ctgctgtggc gccaggaaat gggcggtaac ataacccgcg ttgagagcga gaataaagta gttattcttg actccttcga gccgctgcaa gcagaagaag acgaacgtga agtttctgtg cctgctgaaa tcctgcgtcg ttcccgtaaa ttccctcgtg ccatgccgat ctgggcacgc cctgactaca acccgccgct gctggaatcc tggaaagatc ctgattacgt tccgcctgta gttcacggct gtcctctgcc acctgctaaa gctccaccga tcccaccgcc tcgtcgcaag cgtaccgttg tgttgtccga atccactgtt tctagcgcct tagctgaact ggcaactaag accttcggta gctccgagtc ctccgcagtt gattctggta ccgctaccgc ctctcctgac cagccttccg atgatggtga tgccggtagc gacgtagagt cctacagcag catgccgcct ctcgaaggtg aaccgggtga tcctgacctg agcgatggtt cttggagcac tgttagcgaa gaagcctcag aagacgtagt ttgctgttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for HevcB.17238.b.A16.GE31369
HARVESTED ON: 2/8/2010
SEQUENCED ON: 2/14/2011
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFFSCQRGY KGVWRGDGIM QTTCPCGAQI TGHVKNGSMR IVGPRTCSNT WHGTFPINAY TTGPCTPSPA PNYSRALWRV AAEEYVEVTR VGDFHYVTGM TTDNVKCPCQ VPAPEFFTEV DGVRLHRYAP ACKPLLREEV TFLVGLNQYL VGSQLPCEPE PDVAV
Validated NT Sequence
ttattaaact gcaacgtcag gttctggttc gcacggcagc tgagaaccca cgaggtactg gttcaggcca actaagaaag ttacctcttc acgcagcagc ggtttacagg ccggtgcgta gcggtgcaga cgaacgccgt cgacttcggt gaagaattct ggtgcaggaa cctggcacgg acatttaacg ttatcggtgg tcataccggt cacgtaatgg aaatcaccga cacgggtaac ctccacgtac tcttctgcag ctacacgcca gagtgcacgg ctgtagttcg gagccggaga aggagtacaa gggccagtgg tgtaggcgtt gatagggaaa gtgccgtgcc aagtgttgct gcaagtacgc ggaccaacta tacgcatgct accattcttc acgtgaccgg taatctgtgc accgcatggg caagtagtct gcattatacc gtcaccacgc catacgcctt tatagccacg ctggcagcta aagaacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFFSCQRGY KGVWRGDGIM QTTCPCGAQI TGHVKNGSMR IVGPRTCSNT WHGTFPINAY TTGPCTPSPA PNYSRALWRV AAEEYVEVTR VGDFHYVTGM TTDNVKCPCQ VPAPEFFTEV DGVRLHRYAP ACKPLLREEV TFLVGLNQYL VGSQLPCEPE PDVAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttctttagc tgccagcgtg gctataaagg cgtatggcgt ggtgacggta taatgcagac tacttgccca tgcggtgcac agattaccgg tcacgtgaag aatggtagca tgcgtatagt tggtccgcgt acttgcagca acacttggca cggcactttc cctatcaacg cctacaccac tggcccttgt actccttctc cggctccgaa ctacagccgt gcactctggc gtgtagctgc agaagagtac gtggaggtta cccgtgtcgg tgatttccat tacgtgaccg gtatgaccac cgataacgtt aaatgtccgt gccaggttcc tgcaccagaa ttcttcaccg aagtcgacgg cgttcgtctg caccgctacg caccggcctg taaaccgctg ctgcgtgaag aggtaacttt cttagttggc ctgaaccagt acctcgtggg ttctcagctg ccgtgcgaac cagaacctga cgttgcagtt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa