SSGCID: Target Page

HepyC.20390.a

Methyl-accepting chemotaxis protein (TlpD)

CENTER ID:	HepyC.20390.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.20390.a.B1.GE43603	full length		1	433

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.20390.a.B1.PW38870	full length		1	433

External Resources

RESOURCE	REFERENCE ID
UniProt:	B5Z6X0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFGNKQLQLQ ISQKDTEIME LKKEVNLYQS LLNLCLHEGF VGIKNNKVVF KSGNLASLNN LEEQSIHFKE NAESVNLQGV SYSLKSQNID GVQYFSLAKN TSCVGEYHKN DLFKTFCASL KEGLENAQES MQYFHQETGL LLNAAKNGEV HSTEGLGTVN KTGQDIESLY EKMQNATSLA DSLNQRSNEI TQVISLIDDI AEQTNLLALN AAIEAARAGE HGRGFAVVAD EVRKLAEKTQ KATKEIAVVV KSMQQEANDI QTNTHDINSI VGSIKGDVEE LKSTVKNNMI VAQAAKYTIY NVNNRVFCGL AKLDHVVFKN NLYGMVFGLN SFDITSHKNC RLGKWYYEGA GKENFSNTSG YRALESHHAS VHAEANDLVK AVQEDHITDS KYLEHKVHLM EDSAKHVKEN IDKMFYEKQD ELNKIIEKIQ KGE

NT Sequence

atgtttggga ataagcagtt acagcttcaa atcagtcaaa aagatactga aattatggag ttaaaaaagg aagtcaatct ctatcaaagc cttttaaatt tgtgcttgca tgaaggtttt gtaggtatta aaaacaataa agtcgttttt aaaagcggga atcttgcaag cttaaacaat ttagaagaac aaagcattca ttttaaagaa aacgcagaga gcgttaattt acaaggcgtt tcttattctt tgaaaagcca aaatattgac ggcgtgcagt atttttcatt ggctaaaaac acaagttgtg tgggggaata ccataaaaat gatttgttta agactttttg cgcgagctta aaagaaggct tagagaacgc gcaagaaagc atgcagtatt tccatcaaga aacaggcttg ctcttaaatg cggctaaaaa tggcgaagtg cattctactg aaggattagg gaccgttaat aaaacgggtc aagacattga atcgctttat gaaaagatgc aaaacgccac ttcattagcg gactctctca accaacggag caatgaaatc actcaagtca tttctttgat tgatgatatt gcagaacaaa ccaatctctt agccctaaac gccgctattg aggccgcacg agcgggcgaa catgggagag ggtttgcggt ggtggctgat gaggtgagaa aactcgctga aaaaacccaa aaagccacta aagaaatcgc tgttgtcgtt aaaagcatgc aacaagaagc caacgacatt caaaccaaca cccacgatat taattctatt gtaggttcta ttaagggcga tgtggaagag cttaaatcca ccgtaaaaaa taacatgatt gtcgcgcaag cggcaaaata caccatctac aatgtcaata accgggtgtt ttgcggtcta gccaagcttg atcatgtggt ctttaaaaac aatctttatg gcatggtttt tggcctcaac tcctttgata ttaccagcca taagaattgc cgcttaggca aatggtatta tgagggcgcg ggcaaagaga atttttccaa cacttcaggc tatagagctt tagaaagcca ccatgcgagc gtgcatgctg aagctaatga tttggttaaa gccgttcaag aagaccacat taccgattca aaatacctag aacataaagt gcatttaatg gaagatagcg ctaaacatgt caaagaaaat attgataaga tgttttacga aaaacaagat gaactcaata aaatcattga aaaaattcaa aaaggcgaat ga

Details for HepyC.20390.a.B1.GE43603

HARVESTED ON:	2/3/2020
SEQUENCED ON:	2/13/2020
EXPECTED MW:	49kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMF GNKQLQLQIS QKDTEIMELK KEVNLYQSLL NLCLHEGFVG IKNNKVVFKS GNLASLNNLE EQSIHFKENA ESVNLQGVSY SLKSQNIDGV QYFSLAKNTS CVGEYHKNDL FKTFCASLKE GLENAQESMQ YFHQETGLLL NAAKNGEVHS TEGLGTVNKT GQDIESLYEK MQNATSLADS LNQRSNEITQ VISLIDDIAE QTNLLALNAA IEAARAGEHG RGFAVVADEV RKLAEKTQKA TKEIAVVVKS MQQEANDIQT NTHDINSIVG SIKGDVEELK STVKNNMIVA QAAKYTIYNV NNRVFCGLAK LDHVVFKNNL YGMVFGLNSF DITSHKNCRL GKWYYEGAGK ENFSNTSGYR ALESHHASVH AEANDLVKAV QEDHITDSKY LEHKVHLMED SAKHVKENID KMFYEKQDEL NKIIEKIQKG E

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg ggaataagca gttacagctt caaatcagtc aaaaagatac tgaaattatg gagttaaaaa aggaagtcaa tctctatcaa agccttttaa atttgtgctt gcatgaaggt tttgtaggta ttaaaaacaa taaagtcgtt tttaaaagcg ggaatcttgc aagcttaaac aatttagaag aacaaagcat tcattttaaa gaaaacgcag agagcgttaa tttacaaggc gtttcttatt ctttgaaaag ccaaaatatt gacggcgtgc agtatttttc attggctaaa aacacaagtt gtgtggggga ataccataaa aatgatttgt ttaagacttt ttgcgcgagc ttaaaagaag gcttagagaa cgcgcaagaa agcatgcagt atttccatca agaaacaggc ttgctcttaa atgcggctaa aaatggcgaa gtgcattcta ctgaaggatt agggaccgtt aataaaacgg gtcaagacat tgaatcgctt tatgaaaaga tgcaaaacgc cacttcatta gcggactctc tcaaccaacg gagcaatgaa atcactcaag tcatttcttt gattgatgat attgcagaac aaaccaatct cttagcccta aacgccgcta ttgaggccgc acgagcgggc gaacatggga gagggtttgc ggtggtggct gatgaggtga gaaaactcgc tgaaaaaacc caaaaagcca ctaaagaaat cgctgttgtc gttaaaagca tgcaacaaga agccaacgac attcaaacca acacccacga tattaattct attgtaggtt ctattaaggg cgatgtggaa gagcttaaat ccaccgtaaa aaataacatg attgtcgcgc aagcggcaaa atacaccatc tacaatgtca ataaccgggt gttttgcggt ctagccaagc ttgatcatgt ggtctttaaa aacaatcttt atggcatggt ttttggcctc aactcctttg atattaccag ccataagaat tgccgcttag gcaaatggta ttatgagggc gcgggcaaag agaatttttc caacacttca ggctatagag ctttagaaag ccaccatgcg agcgtgcatg ctgaagctaa tgatttggtt aaagccgttc aagaagacca cattaccgat tcaaaatacc tagaacataa agtgcattta atggaagata gcgctaaaca tgtcaaagaa aatattgata agatgtttta cgaaaaacaa gatgaactca ataaaatcat tgaaaaaatt caaaaaggcg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for HepyC.20390.a.B1.PW38870

PURIFICATION DATe:	7/13/2020
CONCENTRATION:	11mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG GGAATAAGCA GTTACAGCTT CAAATCAGTC AAAAAGATAC TGAAATTATG GAGTTAAAAA AGGAAGTCAA TCTCTATCAA AGCCTTTTAA ATTTGTGCTT GCATGAAGGT TTTGTAGGTA TTAAAAACAA TAAAGTCGTT TTTAAAAGCG GGAATCTTGC AAGCTTAAAC AATTTAGAAG AACAAAGCAT TCATTTTAAA GAAAACGCAG AGAGCGTTAA TTTACAAGGC GTTTCTTATT CTTTGAAAAG CCAAAATATT GACGGCGTGC AGTATTTTTC ATTGGCTAAA AACACAAGTT GTGTGGGGGA ATACCATAAA AATGATTTGT TTAAGACTTT TTGCGCGAGC TTAAAAGAAG GCTTAGAGAA CGCGCAAGAA AGCATGCAGT ATTTCCATCA AGAAACAGGC TTGCTCTTAA ATGCGGCTAA AAATGGCGAA GTGCATTCTA CTGAAGGATT AGGGACCGTT AATAAAACGG GTCAAGACAT TGAATCGCTT TATGAAAAGA TGCAAAACGC CACTTCATTA GCGGACTCTC TCAACCAACG GAGCAATGAA ATCACTCAAG TCATTTCTTT GATTGATGAT ATTGCAGAAC AAACCAATCT CTTAGCCCTA AACGCCGCTA TTGAGGCCGC ACGAGCGGGC GAACATGGGA GAGGGTTTGC GGTGGTGGCT GATGAGGTGA GAAAACTCGC TGAAAAAACC CAAAAAGCCA CTAAAGAAAT CGCTGTTGTC GTTAAAAGCA TGCAACAAGA AGCCAACGAC ATTCAAACCA ACACCCACGA TATTAATTCT ATTGTAGGTT CTATTAAGGG CGATGTGGAA GAGCTTAAAT CCACCGTAAA AAATAACATG ATTGTCGCGC AAGCGGCAAA ATACACCATC TACAATGTCA ATAACCGGGT GTTTTGCGGT CTAGCCAAGC TTGATCATGT GGTCTTTAAA AACAATCTTT ATGGCATGGT TTTTGGCCTC AACTCCTTTG ATATTACCAG CCATAAGAAT TGCCGCTTAG GCAAATGGTA TTATGAGGGC GCGGGCAAAG AGAATTTTTC CAACACTTCA GGCTATAGAG CTTTAGAAAG CCACCATGCG AGCGTGCATG CTGAAGCTAA TGATTTGGTT AAAGCCGTTC AAGAAGACCA CATTACCGAT TCAAAATACC TAGAACATAA AGTGCATTTA ATGGAAGATA GCGCTAAACA TGTCAAAGAA AATATTGATA AGATGTTTTA CGAAAAACAA GATGAACTCA ATAAAATCAT TGAAAAAATT CAAAAAGGCG AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT