SSGCID: Target Page

HepyC.19496.a

Peptidyl-prolylcis-transisomerase D

CENTER ID:	HepyC.19496.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.19496.a.B21.GE41750	soluble domain		51	505

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.19496.a.B21.PS38471	soluble domain		51	505

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1001
UniProt:	B5Z7X9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFKPEFHIFY FLKGIRVLMI EWMQNHRKYL VVTIWISTIA FIAAGMIGWG QYSFSLDSDS AAKVGQIKIS QEELAQEYRR LKDAYAESIP DFKELTEDQI KAMHLEKSAL DSLINQALLR NLALDLGLGA TKQEVAKEIR KTSVFQKDGV FDEELYKNIL KQSHYRPKHF EESVERLLIL QKISTLFPKT TTPLEQSSLS LWAKLQDKLD ILILNPNDVK ISLNEEEMKK YYESHKKDFK KPTSFKTRSL YFDAGLEKTD LKELEEYYHK NKVSYLDKEG KLQDFKSVQE QVKHDLSMQK ANEKALRSYI ALKKANVQNY TTQDFEENNS PYTAEITQKL TALKPLEILK PEPFKDGFIV VQLISQIKDE LQNFDEAKSA LKTRLTQEKT LMALQTLAKE KLKDFKGKSV GYVSPNFGGT ISELNQEESA KFINTLFNRQ EKKGFITIGN KVVLYQITEQ NFNHSFSAEE SQYMQRLVNS TKTDFFDKAL IEELKKRYKI VKYIQ

NT Sequence

ttgtttaaac ctgaatttca cattttttat tttttaaaag ggattagagt tcttatgatt gaatggatgc aaaatcatag aaagtattta gtggttacaa tatggataag cacgatcgct tttattgccg ccgggatgat aggctggggg caatacagct tttctttaga tagcgatagc gctgccaaag tgggacagat taagatttct caagaagaat tagcccaaga ataccgccgc cttaaagacg cctatgctga gtctatccct gattttaaag aactcactga agatcaaatc aaagccatgc atttagaaaa aagcgctcta gattcgctca tcaatcaagc cttattgagg aatctcgctt tagatttagg acttggcgct acgaagcaag aagtggccaa agagatcaga aaaacgagcg tttttcaaaa agatggcgtt tttgatgaag aattgtataa gaatatctta aaacaaagcc attaccgccc caagcatttt gaagaaagcg ttgaaaggct tttaatcctt caaaaaatca gcactctctt ccccaaaacc actacccctt tggagcaatc cagtttatcg ctttgggcaa aattgcaaga caaattagac attcttatcc taaaccctaa tgatgttaaa atctctctta atgaagaaga gatgaaaaaa tattacgagt cccataaaaa ggattttaaa aagcccacaa gctttaaaac acgctcttta tattttgacg ctggtttaga aaaaactgat ttgaaagagt tggaggaata ctaccataaa aacaaggtgt cttatttgga caaagagggg aaattacagg attttaaaag cgttcaagag caagtcaagc atgatttaag catgcaaaaa gcgaatgaaa aagccctaag gagttatatt gctctaaaaa aagcgaacgt gcaaaactac accacgcaag attttgaaga aaacaactcc ccctatactg ctgaaatcac gcaaaaactc accgctctca agccccttga aatcctaaaa ccagagcctt ttaaagatgg ttttatcgtg gtgcagctca tctctcaaat taaagacgaa ttgcagaatt ttgatgaagc caaaagcgct cttaaaaccc gcctaactca agaaaaaacc cttatggcgt tgcaaacatt agctaaagaa aagcttaagg attttaaagg gaaaagcgta ggctatgtaa gccctaattt tggaggcact attagcgaac ttaaccaaga agaaagcgct aagtttatca acaccctttt taaccgccag gaaaaaaagg ggtttattac tattggtaat aaagtggtgc tttatcaaat cacagagcaa aatttcaacc actcctttag cgcagaagaa agccagtata tgcagcgttt agtcaatagc actaaaacgg atttttttga taaagcgttg atagaagaat tgaaaaaacg ctataagata gtcaaataca ttcaataa

Details for HepyC.19496.a.B21.GE41750

HARVESTED ON:	6/19/2017
SEQUENCED ON:	6/22/2017
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHQY SFSLDSDSAA KVGQIKISQE ELAQEYRRLK DAYAESIPDF KELTEDQIKA MHLEKSALDS LINQALLRNL ALDLGLGATK QEVAKEIRKT SVFQKDGVFD EELYKNILKQ SHYRPKHFEE SVERLLILQK ISTLFPKTTT PLEQSSLSLW AKLQDKLDIL ILNPNDVKIS LNEEEMKKYY ESHKKDFKKP TSFKTRSLYF DAGLEKTDLK ELEEYYHKNK VSYLDKEGKL QDFKSVQEQV KHDLSMQKAN EKALRSYIAL KKANVQNYTT QDFEENNSPY TAEITQKLTA LKPLEILKPE PFKDGFIVVQ LISQIKDELQ NFDEAKSALK TRLTQEKTLM ALQTLAKEKL KDFKGKSVGY VSPNFGGTIS ELNQEESAKF INTLFNRQEK KGFITIGNKV VLYQITEQNF NHSFSAEESQ YMQRLVNSTK TDFFDKALIE ELKKRYKIVK YIQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaataca gcttttcttt agatagcgat agcgctgcca aagtgggaca gattaagatt tctcaagaag aattagccca agaataccgc cgccttaaag acgcctatgc tgagtctatc cctgatttta aagaactcac tgaagatcaa atcaaagcca tgcatttaga aaaaagcgct ctagattcgc tcatcaatca agccttattg aggaatctcg ctttagattt aggacttggc gctacgaagc aagaagtggc caaagagatc agaaaaacga gcgtttttca aaaagatggc gtttttgatg aagaattgta taagaatatc ttaaaacaaa gccattaccg ccccaagcat tttgaagaaa gcgttgaaag gcttttaatc cttcaaaaaa tcagcactct cttccccaaa accactaccc ctttggagca atccagttta tcgctttggg caaaattgca agacaaatta gacattctta tcctaaaccc taatgatgtt aaaatctctc ttaatgaaga agagatgaaa aaatattacg agtcccataa aaaggatttt aaaaagccca caagctttaa aacacgctct ttatattttg acgctggttt agaaaaaact gatttgaaag agttggagga atactaccat aaaaacaagg tgtcttattt ggacaaagag gggaaattac aggattttaa aagcgttcaa gagcaagtca agcatgattt aagcatgcaa aaagcgaatg aaaaagccct aaggagttat attgctctaa aaaaagcgaa cgtgcaaaac tacaccacgc aagattttga agaaaacaac tccccctata ctgctgaaat cacgcaaaaa ctcaccgctc tcaagcccct tgaaatccta aaaccagagc cttttaaaga tggttttatc gtggtgcagc tcatctctca aattaaagac gaattgcaga attttgatga agccaaaagc gctcttaaaa cccgcctaac tcaagaaaaa acccttatgg cgttgcaaac attagctaaa gaaaagctta aggattttaa agggaaaagc gtaggctatg taagccctaa ttttggaggc actattagcg aacttaacca agaagaaagc gctaagttta tcaacaccct ttttaaccgc caggaaaaaa aggggtttat tactattggt aataaagtgg tgctttatca aatcacagag caaaatttca accactcctt tagcgcagaa gaaagccagt atatgcagcg tttagtcaat agcactaaaa cggatttttt tgataaagcg ttgatagaag aattgaaaaa acgctataag atagtcaaat acattcaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for HepyC.19496.a.B21.PS38471

PURIFICATION DATe:	7/3/2018
CONCENTRATION:	13.4mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAATACA GCTTTTCTTT AGATAGCGAT AGCGCTGCCA AAGTGGGACA GATTAAGATT TCTCAAGAAG AATTAGCCCA AGAATACCGC CGCCTTAAAG ACGCCTATGC TGAGTCTATC CCTGATTTTA AAGAACTCAC TGAAGATCAA ATCAAAGCCA TGCATTTAGA AAAAAGCGCT CTAGATTCGC TCATCAATCA AGCCTTATTG AGGAATCTCG CTTTAGATTT AGGACTTGGC GCTACGAAGC AAGAAGTGGC CAAAGAGATC AGAAAAACGA GCGTTTTTCA AAAAGATGGC GTTTTTGATG AAGAATTGTA TAAGAATATC TTAAAACAAA GCCATTACCG CCCCAAGCAT TTTGAAGAAA GCGTTGAAAG GCTTTTAATC CTTCAAAAAA TCAGCACTCT CTTCCCCAAA ACCACTACCC CTTTGGAGCA ATCCAGTTTA TCGCTTTGGG CAAAATTGCA AGACAAATTA GACATTCTTA TCCTAAACCC TAATGATGTT AAAATCTCTC TTAATGAAGA AGAGATGAAA AAATATTACG AGTCCCATAA AAAGGATTTT AAAAAGCCCA CAAGCTTTAA AACACGCTCT TTATATTTTG ACGCTGGTTT AGAAAAAACT GATTTGAAAG AGTTGGAGGA ATACTACCAT AAAAACAAGG TGTCTTATTT GGACAAAGAG GGGAAATTAC AGGATTTTAA AAGCGTTCAA GAGCAAGTCA AGCATGATTT AAGCATGCAA AAAGCGAATG AAAAAGCCCT AAGGAGTTAT ATTGCTCTAA AAAAAGCGAA CGTGCAAAAC TACACCACGC AAGATTTTGA AGAAAACAAC TCCCCCTATA CTGCTGAAAT CACGCAAAAA CTCACCGCTC TCAAGCCCCT TGAAATCCTA AAACCAGAGC CTTTTAAAGA TGGTTTTATC GTGGTGCAGC TCATCTCTCA AATTAAAGAC GAATTGCAGA ATTTTGATGA AGCCAAAAGC GCTCTTAAAA CCCGCCTAAC TCAAGAAAAA ACCCTTATGG CGTTGCAAAC ATTAGCTAAA GAAAAGCTTA AGGATTTTAA AGGGAAAAGC GTAGGCTATG TAAGCCCTAA TTTTGGAGGC ACTATTAGCG AACTTAACCA AGAAGAAAGC GCTAAGTTTA TCAACACCCT TTTTAACCGC CAGGAAAAAA AGGGGTTTAT TACTATTGGT AATAAAGTGG TGCTTTATCA AATCACAGAG CAAAATTTCA ACCACTCCTT TAGCGCAGAA GAAAGCCAGT ATATGCAGCG TTTAGTCAAT AGCACTAAAA CGGATTTTTT TGATAAAGCG TTGATAGAAG AATTGAAAAA ACGCTATAAG ATAGTCAAAT ACATTCAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT