HepyC.19495.a

Outer membrane protein (HopK-1)

CENTER ID: HepyC.19495.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19495.a.B21.GE41749 mature protein 21 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.473
UniProt: B5Z6K0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQKALLHSSF FLPLFLSYCI AEENGAYASV GFEYSISHAV EHNDPFLNQE RIQTISNAQN KIYKLNQVKN EITNMQKTFN YINNALKNNS KLTPTEMQAE QYYLQSTLQN IEKIVTLSGG VASNPQLAQA LEKMQEPITN PLELAERLKN LELQFAQSQN RMLSSLSSQI AAISNSLNAL DPNSYSKNVS SMYGVSLSVG YKHFFTKKKN QGLRYYLFYD YGYTNFGFVG NGFDGLGKMN NHLYGLGIDY LYNFIDNAKK HSSVGFYAGF ALAGSSWVGS GLGMWVSQMG FINNYLTDYQ AKMHTSFFQI PLNFGVRVNV NRHNGFEMGL KIPLAVNSFY ETHGKGLNAS LFFKRLVMFN VSYVYSF
NT Sequence
atgcaaaaag ccttattaca ttcatcattc tttttacctt tgtttttatc ttattgtatc gctgaagaaa atggggcgta tgcgagcgtg gggtttgaat attccattag tcatgccgtt gaacacaatg accccttttt gaatcaagaa cgcatccaaa cgatttctaa cgctcaaaat aaaatctata aactcaatca agttaaaaat gaaatcacaa acatgcaaaa aacctttaat tacatcaaca acgctttaaa aaacaactcc aaattaaccc ccactgaaat gcaagccgag caatactacc tccaatccac ccttcaaaac attgaaaaaa tagtcacact tagcgggggc gttgcatcta acccacaatt agcccaagcg ttagaaaaaa tgcaagaacc cattactaac cctttagaat tagcagaaag attaaagaat ttagaattgc aatttgctca atctcaaaac cgcatgcttt cttctttatc ttctcaaatc gctgcaattt caaattcttt gaacgcgctt gatcccaact cttattctaa aaacgtttca agcatgtatg gggtgagttt gagcgtgggt tataagcatt tctttaccaa gaaaaaaaat caagggttgc gctattattt gttctatgac tatggttaca ctaattttgg ttttgtgggc aatggctttg atggtttagg caaaatgaat aaccacctct atgggcttgg gatagattat ctttataatt tcattgataa tgcaaaaaaa cattcgagcg tgggttttta tgcaggtttt gctttagcgg ggagttcgtg ggtagggagt ggtttaggca tgtgggtgag ccaaatgggt tttattaaca attatttgac ggattatcag gctaaaatgc acacgagttt tttccaaatc cctttgaatt ttggggttcg tgtgaatgtc aataggcata atggctttga aatgggctta aaaatccctt tagcagtcaa ttccttttat gaaacgcatg gcaaagggtt aaacgcttcc ctctttttca aacgccttgt catgtttaat gtgagttatg tttatagttt ttag
Details for HepyC.19495.a.B21.GE41749
HARVESTED ON: data unavailable
SEQUENCED ON: 6/22/2017
EXPECTED MW: 40kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 80
Validated AA Sequence
MQKTFNYINN ALKNNSKLTP TEMQAEQYYL QSTLQNIEKI VTLSGGVASN PQLAQALEKM QEPITNPLEL AERLKNLELQ FAQSQNRMLS SLSSQIAAIS NSLNALDPNS YSKNVSSMYG VSLSVGYKHF FTKKKNQGLR YYLFYDYGYT NFGFVGNGFD GLGKMNNHLY GLGIDYLYNF IDNAKKHSSV GFYAGFALAG SSWVGSGLGM WVSQMGFINN YLTDYQAKMH TSFFQIPLNF GVRVNVNRHN GFEMGLKIPL AVNSFYETHG KGLNASLFFK RLVM
Validated NT Sequence
acatgacaag gcgtttgaaa aagagggaag cgtttaaccc tttgccatgc gtttcataaa aggaattgac tgctaaaggg atttttaagc ccatttcaaa gccattatgc ctattgacat tcacacgaac cccaaaattc aaagggattt ggaaaaaact cgtgtgcatt ttagcctgat aatccgtcaa ataattgtta ataaaaccca tttggctcac ccacatgcct aaaccactcc ctacccacga actccccgct aaagcaaaac ctgcataaaa acccacgctc gaatgttttt ttgcattatc aatgaaatta taaagataat ctatcccaag cccatagagg tggttattca ttttgcctaa accatcaaag ccattgccca caaaaccaaa attagtgtaa ccatagtcat agaacaaata atagcgcaac ccttgatttt ttttcttggt aaagaaatgc ttataaccca cgctcaaact caccccatac atgcttgaaa cgtttttaga ataagagttg ggatcaagcg cgttcaaaga atttgaaatt gcagcgattt gagaagataa agaagaaagc atgcggtttt gagattgagc aaattgcaat tctaaattct ttaatctttc tgctaattct aaagggttag taatgggttc ttgcattttt tctaacgctt gggctaattg tgggttagat gcaacgcccc cgctaagtgt gactattttt tcaatgtttt gaagggtgga ttggaggtag tattgctcgg cttgcatttc agtgggggtt aatttggagt tgttttttaa agcgttgttg atgtaattaa aggttttttg catgtttgtg atttcatttt taacttgatt gagtttatag attttatttt gagcgttaga aatcgtttgg atgcgttctt gattcaaaaa ggggtcattg tgttcaacgg catgactaat ggaatattca aaccccacgc tcgcatacgc cccattttct nnnnnnnnat ggtggtggtg gtggtg
Expected Protein Sequence
MAHHHHHHAE ENGAYASVGF EYSISHAVEH NDPFLNQERI QTISNAQNKI YKLNQVKNEI TNMQKTFNYI NNALKNNSKL TPTEMQAEQY YLQSTLQNIE KIVTLSGGVA SNPQLAQALE KMQEPITNPL ELAERLKNLE LQFAQSQNRM LSSLSSQIAA ISNSLNALDP NSYSKNVSSM YGVSLSVGYK HFFTKKKNQG LRYYLFYDYG YTNFGFVGNG FDGLGKMNNH LYGLGIDYLY NFIDNAKKHS SVGFYAGFAL AGSSWVGSGL GMWVSQMGFI NNYLTDYQAK MHTSFFQIPL NFGVRVNVNR HNGFEMGLKI PLAVNSFYET HGKGLNASLF FKRLVMFNVS YVYSF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgaag aaaatggggc gtatgcgagc gtggggtttg aatattccat tagtcatgcc gttgaacaca atgacccctt tttgaatcaa gaacgcatcc aaacgatttc taacgctcaa aataaaatct ataaactcaa tcaagttaaa aatgaaatca caaacatgca aaaaaccttt aattacatca acaacgcttt aaaaaacaac tccaaattaa cccccactga aatgcaagcc gagcaatact acctccaatc cacccttcaa aacattgaaa aaatagtcac acttagcggg ggcgttgcat ctaacccaca attagcccaa gcgttagaaa aaatgcaaga acccattact aaccctttag aattagcaga aagattaaag aatttagaat tgcaatttgc tcaatctcaa aaccgcatgc tttcttcttt atcttctcaa atcgctgcaa tttcaaattc tttgaacgcg cttgatccca actcttattc taaaaacgtt tcaagcatgt atggggtgag tttgagcgtg ggttataagc atttctttac caagaaaaaa aatcaagggt tgcgctatta tttgttctat gactatggtt acactaattt tggttttgtg ggcaatggct ttgatggttt aggcaaaatg aataaccacc tctatgggct tgggatagat tatctttata atttcattga taatgcaaaa aaacattcga gcgtgggttt ttatgcaggt tttgctttag cggggagttc gtgggtaggg agtggtttag gcatgtgggt gagccaaatg ggttttatta acaattattt gacggattat caggctaaaa tgcacacgag ttttttccaa atccctttga attttggggt tcgtgtgaat gtcaataggc ataatggctt tgaaatgggc ttaaaaatcc ctttagcagt caattccttt tatgaaacgc atggcaaagg gttaaacgct tccctctttt tcaaacgcct tgtcatgttt aatgtgagtt atgtttatag tttttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat