HepyC.19492.a

Cadmium-transporting ATPase

CENTER ID: HepyC.19492.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19492.a.B2.GE41346 soluble domain #1 174 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19492.a.B2.PW38300 soluble domain #1 174 305

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.803
UniProt: B5Z7F3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQEYHIHNLD CPDCASKLER DLNKLDYVKK AQINFSTSKL FLDTSDFEKV KDFIKQNEPH LSLSFKEAAE KPLSFTPLIA TIAVFLGAIL ILHLNPSPLI EKAMFFVLAL VYLVSGKDVI LGAFRGLRKG QFFDENALML IATIAAFCVG AYEESVSIMV FYSAGEFLQK LAIARSKKSL KALVDVAPNL AYLKKGDALV SVAPEDLRIN DIVVVKVGEK VPVDGVVIKG ESLLDERALS GESMPVNVSE RSKVLGGSLN LKAVLEIQVE KMYKDSSIAK VVDLVQQATN EKSETEKFIT KFSRYYTPSV LCIALMIAIL PPLFSMGSFD EWIYRGLVAL MVSCPCALVI SVPLGYFGGV GAASRKGILM KGVHVLEVLT QAKSIAFDKT GTLTKGVFKV TDIVPQNGHS KEEVLHYASC SQLLSTHPIA LSIQKACEEM LKDDKHQHDI KNYEEVSGMG VKAQCHTDLI IAGNEKMLDQ FHIAHSPSKE NGTIVHVAFN QTYIGYIVIS DEIKDDAIEC LRDLKAQGIE NFCILSGDRK SATESIAQTL GCEYYASLLP EEKTSVFKTF KERYKAPAIF VGDGINDAPT LASADVGIGM GKGSELSKQS ADIVITNDSL SSLVKVLAIA KKTKSIIWQN ILFALGIKAV FIVLGLMGVA SLWEAVFGDV GVTLLALANS MRAMRA
NT Sequence
atgcaagaat accacattca caatttggat tgccctgatt gcgcgtctaa attagaaagg gatttaaaca aactggacta tgtgaaaaag gctcaaatca atttcagcac cagtaagctg tttttggata cgagcgattt tgaaaaagtt aaggatttca tcaagcagaa tgaaccgcat ttgagcctgt cttttaaaga ggccgcagaa aagcccttga gttttacccc actcatcgct acgatcgctg tctttttggg cgcgatttta atcttacacc taaaccctag ccctttgatt gaaaaagcta tgtttttcgt gttggcttta gtgtatttag tgagcggtaa agatgtgatt ttaggggcgt ttcgtgggct tagaaaaggg caattttttg atgaaaacgc tttgatgctc attgcgacta ttgcggcttt ttgcgtgggg gcttatgaag agagcgtctc tattatggtg ttttattcag cgggcgaatt tttgcaaaaa ctcgctatcg ctcgctctaa aaaatccctt aaggctttag tggatgtcgc tcctaatttg gcttatttga aaaagggcga tgcattagtg agcgttgcac ctgaagattt aagaattaat gacatcgtgg tggtgaaagt cggcgaaaaa gtgcctgtgg atggcgtggt gatcaagggt gaaagtttgc tagatgaaag ggcgttgagt ggggagtcta tgcctgttaa tgtcagcgaa cgctctaaag ttttaggggg gagcttgaat ttaaaagcgg tccttgaaat ccaagtggaa aaaatgtata aagattcttc tatcgctaaa gtggtggatt tagtccaaca agccacgaat gaaaagagcg aaaccgagaa attcatcact aaattttcac gctactacac cccaagcgtt ttatgcattg cgctaatgat cgctatatta ccgcccttat tttctatggg gagctttgat gagtggattt atagggggct tgtggcttta atggtgagct gtccttgcgc gttagtgatt tctgtgcctt tagggtattt tgggggcgtg ggagcggcga gccgaaaggg tattttaatg aaaggcgtgc atgttttaga ggtgcttacc caagctaaaa gcatcgcctt tgataaaacc ggcactttaa ctaaaggcgt ttttaaagta acagatattg tgccgcaaaa cgggcattct aaagaagaag ttttgcatta cgcttcttgt tcgcagctct tatccacgca cccgatcgct ttatccattc aaaaagcatg cgaagaaatg ttaaaggacg ataagcacca gcatgacatt aaaaattacg aagaagtgag cgggatgggg gttaaagcgc aatgccatac ggacctaatc atcgcaggga atgaaaaaat gctcgatcaa ttccatatcg cgcacagccc ttccaaagaa aacggcacga tcgtgcatgt ggcttttaac caaacttata tcggctacat cgtcattagc gatgagatta aagatgacgc catagagtgc ttaagggatt taaaagcaca agggatagaa aatttttgca ttttgagcgg ggacagaaaa agcgcgactg agagcatcgc tcaaactcta ggctgtgaat attatgcgag cttgttgcct gaagaaaaaa cgagcgtgtt taaaaccttt aaagaacgct ataaagcccc ggcgattttt gtaggcgatg gcatcaatga cgctccgact ctagcgagcg ctgacgtggg gattggcatg gggaaaggct cagagttgag caagcagagc gcggacattg tgatcactaa tgactcctta agctctttgg tgaaagtttt agcgatcgct aaaaaaacta aaagcattat ttggcaaaat attttgtttg ctttagggat taaggcggtt tttatcgtgc tagggcttat gggggtagcg agcttgtggg aagcggtctt tggcgatgtg ggggttacgc ttttagcctt agccaattcc atgcgcgcaa tgagggctta a
Details for HepyC.19492.a.B2.GE41346
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 15kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RSKKSLKALV DVAPNLAYLK KGDALVSVAP EDLRINDIVV VKVGEKVPVD GVVIKGESLL DERALSGESM PVNVSERSKV LGGSLNLKAV LEIQVEKMYK DSSIAKVVDL VQQATNEKSE TEKFITKFSR Y
Validated NT Sequence
atcttactca cttagtagcg tgaaaattta gtgatgaatt tctcggtttc gctcttttca ttcgtggctt gttggactaa atccaccact ttagcgatag aagaatcttt atacattttt tccacttgga tttcaaggac cgcttttaaa ttcaagctcc cccctaaaac tttagagcgt tcgctgacat taacaggcat agactcccca ctcaacgccc tttcatctag caaactttca cccttgatca ccacgccatc cacaggcact ttttcgccga ctttcaccac cacgatgtca ttaattctta aatcttcagg tgcaacgctc actaatgcat cgcccttttt caaataagcc aaattaggag cgacatccac taaagcctta agggattttt tagagcgagc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHAR SKKSLKALVD VAPNLAYLKK GDALVSVAPE DLRINDIVVV KVGEKVPVDG VVIKGESLLD ERALSGESMP VNVSERSKVL GGSLNLKAVL EIQVEKMYKD SSIAKVVDLV QQATNEKSET EKFITKFSRY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctcgct ctaaaaaatc ccttaaggct ttagtggatg tcgctcctaa tttggcttat ttgaaaaagg gcgatgcatt agtgagcgtt gcacctgaag atttaagaat taatgacatc gtggtggtga aagtcggcga aaaagtgcct gtggatggcg tggtgatcaa gggtgaaagt ttgctagatg aaagggcgtt gagtggggag tctatgcctg ttaatgtcag cgaacgctct aaagttttag gggggagctt gaatttaaaa gcggtccttg aaatccaagt ggaaaaaatg tataaagatt cttctatcgc taaagtggtg gatttagtcc aacaagccac gaatgaaaag agcgaaaccg agaaattcat cactaaattt tcacgctact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for HepyC.19492.a.B2.PW38300
PURIFICATION DATe: 6/12/2017
CONCENTRATION: 26.7mg/ml
OBSERVED MW: 16kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RSKKSLKALV DVAPNLAYLK KGDALVSVAP EDLRINDIVV VKVGEKVPVD GVVIKGESLL DERALSGESM PVNVSERSKV LGGSLNLKAV LEIQVEKMYK DSSIAKVVDL VQQATNEKSE TEKFITKFSR Y
Validated NT Sequence
atcttactca cttagtagcg tgaaaattta gtgatgaatt tctcggtttc gctcttttca ttcgtggctt gttggactaa atccaccact ttagcgatag aagaatcttt atacattttt tccacttgga tttcaaggac cgcttttaaa ttcaagctcc cccctaaaac tttagagcgt tcgctgacat taacaggcat agactcccca ctcaacgccc tttcatctag caaactttca cccttgatca ccacgccatc cacaggcact ttttcgccga ctttcaccac cacgatgtca ttaattctta aatcttcagg tgcaacgctc actaatgcat cgcccttttt caaataagcc aaattaggag cgacatccac taaagcctta agggattttt tagagcgagc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHAR SKKSLKALVD VAPNLAYLKK GDALVSVAPE DLRINDIVVV KVGEKVPVDG VVIKGESLLD ERALSGESMP VNVSERSKVL GGSLNLKAVL EIQVEKMYKD SSIAKVVDLV QQATNEKSET EKFITKFSRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTCGCT CTAAAAAATC CCTTAAGGCT TTAGTGGATG TCGCTCCTAA TTTGGCTTAT TTGAAAAAGG GCGATGCATT AGTGAGCGTT GCACCTGAAG ATTTAAGAAT TAATGACATC GTGGTGGTGA AAGTCGGCGA AAAAGTGCCT GTGGATGGCG TGGTGATCAA GGGTGAAAGT TTGCTAGATG AAAGGGCGTT GAGTGGGGAG TCTATGCCTG TTAATGTCAG CGAACGCTCT AAAGTTTTAG GGGGGAGCTT GAATTTAAAA GCGGTCCTTG AAATCCAAGT GGAAAAAATG TATAAAGATT CTTCTATCGC TAAAGTGGTG GATTTAGTCC AACAAGCCAC GAATGAAAAG AGCGAAACCG AGAAATTCAT CACTAAATTT TCACGCTACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT