HepyC.19487.a

Uncharacterized protein

CENTER ID: HepyC.19487.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19487.a.B2.GE41343 mature protein 20 555
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19487.a.B2.PW38385 mature protein 20 555

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.877
UniProt: B5Z7M3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKQFVILLI ALIAFSGCNM RKYFKPAKHQ IKGEAYFPNH LQESIVSSNR YGAILKNGAV IGDKGLTQLR IGKNFNYESS FLNESQGFFI LAQDCLNKID KKTSKSKVAK TEETELKLKG VEAEVQDKVC HQVELISNNP NASQQSIVIP LETFALSASV KGNLLAVVLA DNSANLYDIT SQKLLFSEKG SPSTTINSLM AMPIFMDTVV VFPMLDGRLL VVDYVHGNPT PIRNIVISSD KFFNNITYLI VDGNNMIAST GKRILSVVSG QEFNYDGDIV DLLYDKGTLY VLTLDGQILQ MDKSLRELNS VKLPFASLNT IVLNHNKLYS LEKRGYVIEV DLNDFDSYNV YKTPTIGSFK FFSSNRLDKG VFYDKNRVYY DRYYLDYNDF KPKLYPVVEK SASKKSQKGE KGNAPIYLQE RHKAKEKPLE ENKVKPRNSG FEEEEVKTRR PEPIKDQNNA IQKGMKENQE NKNAPVSKEG NQKGAKNAPA SKEDNAIKEA PKLSPKEEKR RLKEEKKKAK AEQRAREFEQ RAREHQERDE KELEERRKAL EMNKK
NT Sequence
atgaataaac aatttgtaat cttactcata gccctaattg cctttagcgg ctgtaacatg agaaaatact tcaaacccgc taaacaccaa attaaaggcg aagcgtattt ccctaaccat ttgcaagaaa gtatcgtttc gtctaatcgt tatggagcca ttttgaaaaa tggagcggtt ataggcgata aaggtttaac gcaattaaga atcggtaaga atttcaatta tgaaagcagt tttttaaatg agagtcaggg gttttttatc cttgcgcaag attgtttgaa caagattgat aaaaaaacaa gcaaaagcaa ggtggctaag actgaagaaa cggaattgaa attaaagggc gttgaagcgg aagtccaaga taaagtctgt catcaagtgg aattgattag taataaccct aacgccagcc aacaatctat cgttatccct ttggagactt ttgccttgag cgcgagcgtt aaagggaatc ttttagcggt ggtgttagcg gacaattcag cgaatttata cgacatcact tctcaaaaat tgctttttag cgagaaaggt tccccaagca ccacgatcaa ttctttaatg gcgatgccta tttttatgga tacggtcgtg gtattcccca tgctagatgg gcgcttgtta gtcgtggatt atgtgcatgg aaaccctacg cctattagaa acattgttat cagcagcgat aagtttttta acaatatcac ctaccttatt gtagatggca ataacatgat cgcttctaca gggaaaagga tactctcagt agtgagcggt caagagttca actatgatgg ggatattgtg gatttgcttt atgataaggg gactttatat gtgctcacgc tagacgggca gattttgcaa atggataaga gtttgaggga attaaacagc gtgaaactgc cctttgcttc gctcaatacc attgtattaa accataataa attgtattct ttagaaaagc gcgggtatgt gatagaggtg gatttgaatg attttgattc gtataatgtc tataaaacgc caactatagg cagttttaag tttttttcat ctaatcgttt ggataaaggg gtgttttatg ataaaaatcg ggtgtattat gatcgctact atttagatta taacgatttt aagccaaaac tttatcccgt tgtggaaaaa tcggcatcta aaaaatctca aaaaggcgaa aaagggaacg ctcctattta tttgcaagaa aggcataaag ctaaagaaaa gcctttagaa gaaaacaaag ttaagccaag aaatagcggg tttgaagaag aagaagttaa aaccagaaga cctgagccta ttaaggatca aaataacgct atccaaaaag gcatgaaaga aaatcaagaa aacaaaaacg ctcctgtttc aaaagagggt aaccaaaaag gcgcaaaaaa cgctcctgct tcaaaagagg ataacgctat taaagaagcg ccaaaactca gccctaaaga agaaaaacgc cgcttgaaag aagaaaagaa aaaagccaaa gccgaacaaa gagcgagaga atttgaacaa agagcgagag agcatcaaga aagagatgaa aaagagcttg aagaaagaag aaaagcttta gaaatgaata agaagtag
Details for HepyC.19487.a.B2.GE41343
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 62kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR KYFKPAKHQI KGEAYFPNHL QESIVSSNRY GAILKNGAVI GDKGLTQLRI GKNFNYESSF LNESQGFFIL AQDCLNKIDK KTSKSKVAKT EETELKLKGV EAEVQDKVCH QVELISNNPN ASQQSIVIPL ETFALSASVK GNLLAVVLAD NSANLYDITS QKLLFSEKGS PSTTINSLMA MPIFMDTVVV FPMLDGRLLV VDYVHGNPTP IRNIVISSDK FFNNITYLIV DGNNMIASTG KRILSVVSGQ EFNYDGDIVD LLYDKGTLYV LTLDGQILQM DKSLRELNSV KLPFASLNTI VLNHNKLYSL EKRGYVIEVD LNDFDSYNVY KTPTIGSFKF FSSNRLDKGV FYDKNRVYYD RYYLDYNDFK PKLYPVVEKS ASKKSQKGEK GNAPIYLQER HKAKEKPLEE NKVKPRNSGF EEEEVKTRRP EPIKDQNNAI QKGMKENQEN KNAPVSKEGN QKGAKNAPAS KEDNAIKEAP KLSPKEEKRR LKEEKKKAKA EQRAREFEQR AREHQERDEK ELEERR
Validated NT Sequence
tttcttcttt cttcaagctc tttttcatct ctttcttgat gctctctcgc tctttgttca aattctctcg ctctttgttc ggctttggct tttttctttt cttctttcaa gcggcgtttt tcttctttag ggctgagttt tggcgcttct ttaatagcgt tatcctcttt tgaagcagga gcgttttttg cgcctttttg gttaccctct tttgaaacag gagcgttttt gttttcttga ttttctttca tgcctttttg gatagcgtta ttttgatcct taataggctc aggtcttctg gttttaactt cttcttcttc aaacccgcta tttcttggct taactttgtt ttcttctaaa ggcttttctt tagctttatg cctttcttgc aaataaatag gagcgttccc tttttcgcct ttttgagatt ttttagatgc cgatttttcc acaacgggat aaagttttgg cttaaaatcg ttataatcta aatagtagcg atcataatac acccgatttt tatcataaaa caccccttta tccaaacgat tagatgaaaa aaacttaaaa ctgcctatag ttggcgtttt atagacatta tacgaatcaa aatcattcaa atccacctct atcacatacc cgcgcttttc taaagaatac aatttattat ggtttaatac aatggtattg agcgaagcaa agggcagttt cacgctgttt aattccctca aactcttatc catttgcaaa atctgcccgt ctagcgtgag cacatataaa gtccccttat cataaagcaa atccacaata tccccatcat agttgaactc ttgaccgctc actactgaga gtatcctttt ccctgtagaa gcgatcatgt tattgccatc tacaataagg taggtgatat tgttaaaaaa cttatcgctg ctgataacaa tgtttctaat aggcgtaggg tttccatgca cataatccac gactaacaag cgcccatcta gcatggggaa taccacgacc gtatccataa aaataggcat cgccattaaa gaattgatcg tggtgcttgg ggaacctttc tcgctaaaaa gcaatttttg agaagtgatg tcgtataaat tcgctgaatt gtccgctaac accaccgcta aaagattccc tttaacgctc gcgctcaagg caaaagtctc caaagggata acgatagatt gttggctggc gttagggtta ttactaatca attccacttg atgacagact ttatcttgga cttccgcttc aacgcccttt aatttcaatt ccgtttcttc agtcttagcc accttgcttt tgcttgtttt tttatcaatc ttgttcaaac aatcttgcgc aaggataaaa aacccctgac tctcatttaa aaaactgctt tcataattga aattcttacc gattcttaat tgcgttaaac ctttatcgcc tataaccgct ccatttttca aaatggctcc ataacgatta gacgaaacga tactttcttg caaatggtta gggaaatacg cttcgccttt aatttggtgt ttagcgggtt tgaagtattt tctcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMR KYFKPAKHQI KGEAYFPNHL QESIVSSNRY GAILKNGAVI GDKGLTQLRI GKNFNYESSF LNESQGFFIL AQDCLNKIDK KTSKSKVAKT EETELKLKGV EAEVQDKVCH QVELISNNPN ASQQSIVIPL ETFALSASVK GNLLAVVLAD NSANLYDITS QKLLFSEKGS PSTTINSLMA MPIFMDTVVV FPMLDGRLLV VDYVHGNPTP IRNIVISSDK FFNNITYLIV DGNNMIASTG KRILSVVSGQ EFNYDGDIVD LLYDKGTLYV LTLDGQILQM DKSLRELNSV KLPFASLNTI VLNHNKLYSL EKRGYVIEVD LNDFDSYNVY KTPTIGSFKF FSSNRLDKGV FYDKNRVYYD RYYLDYNDFK PKLYPVVEKS ASKKSQKGEK GNAPIYLQER HKAKEKPLEE NKVKPRNSGF EEEEVKTRRP EPIKDQNNAI QKGMKENQEN KNAPVSKEGN QKGAKNAPAS KEDNAIKEAP KLSPKEEKRR LKEEKKKAKA EQRAREFEQR AREHQERDEK ELEERRKALE MNKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa aatacttcaa acccgctaaa caccaaatta aaggcgaagc gtatttccct aaccatttgc aagaaagtat cgtttcgtct aatcgttatg gagccatttt gaaaaatgga gcggttatag gcgataaagg tttaacgcaa ttaagaatcg gtaagaattt caattatgaa agcagttttt taaatgagag tcaggggttt tttatccttg cgcaagattg tttgaacaag attgataaaa aaacaagcaa aagcaaggtg gctaagactg aagaaacgga attgaaatta aagggcgttg aagcggaagt ccaagataaa gtctgtcatc aagtggaatt gattagtaat aaccctaacg ccagccaaca atctatcgtt atccctttgg agacttttgc cttgagcgcg agcgttaaag ggaatctttt agcggtggtg ttagcggaca attcagcgaa tttatacgac atcacttctc aaaaattgct ttttagcgag aaaggttccc caagcaccac gatcaattct ttaatggcga tgcctatttt tatggatacg gtcgtggtat tccccatgct agatgggcgc ttgttagtcg tggattatgt gcatggaaac cctacgccta ttagaaacat tgttatcagc agcgataagt tttttaacaa tatcacctac cttattgtag atggcaataa catgatcgct tctacaggga aaaggatact ctcagtagtg agcggtcaag agttcaacta tgatggggat attgtggatt tgctttatga taaggggact ttatatgtgc tcacgctaga cgggcagatt ttgcaaatgg ataagagttt gagggaatta aacagcgtga aactgccctt tgcttcgctc aataccattg tattaaacca taataaattg tattctttag aaaagcgcgg gtatgtgata gaggtggatt tgaatgattt tgattcgtat aatgtctata aaacgccaac tataggcagt tttaagtttt tttcatctaa tcgtttggat aaaggggtgt tttatgataa aaatcgggtg tattatgatc gctactattt agattataac gattttaagc caaaacttta tcccgttgtg gaaaaatcgg catctaaaaa atctcaaaaa ggcgaaaaag ggaacgctcc tatttatttg caagaaaggc ataaagctaa agaaaagcct ttagaagaaa acaaagttaa gccaagaaat agcgggtttg aagaagaaga agttaaaacc agaagacctg agcctattaa ggatcaaaat aacgctatcc aaaaaggcat gaaagaaaat caagaaaaca aaaacgctcc tgtttcaaaa gagggtaacc aaaaaggcgc aaaaaacgct cctgcttcaa aagaggataa cgctattaaa gaagcgccaa aactcagccc taaagaagaa aaacgccgct tgaaagaaga aaagaaaaaa gccaaagccg aacaaagagc gagagaattt gaacaaagag cgagagagca tcaagaaaga gatgaaaaag agcttgaaga aagaagaaaa gctttagaaa tgaataagaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for HepyC.19487.a.B2.PW38385
PURIFICATION DATe: 11/6/2017
CONCENTRATION: 18.62mg/ml
OBSERVED MW: 74kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
IMAC->SEC
Validated AA Sequence
MAHHHHHHMR KYFKPAKHQI KGEAYFPNHL QESIVSSNRY GAILKNGAVI GDKGLTQLRI GKNFNYESSF LNESQGFFIL AQDCLNKIDK KTSKSKVAKT EETELKLKGV EAEVQDKVCH QVELISNNPN ASQQSIVIPL ETFALSASVK GNLLAVVLAD NSANLYDITS QKLLFSEKGS PSTTINSLMA MPIFMDTVVV FPMLDGRLLV VDYVHGNPTP IRNIVISSDK FFNNITYLIV DGNNMIASTG KRILSVVSGQ EFNYDGDIVD LLYDKGTLYV LTLDGQILQM DKSLRELNSV KLPFASLNTI VLNHNKLYSL EKRGYVIEVD LNDFDSYNVY KTPTIGSFKF FSSNRLDKGV FYDKNRVYYD RYYLDYNDFK PKLYPVVEKS ASKKSQKGEK GNAPIYLQER HKAKEKPLEE NKVKPRNSGF EEEEVKTRRP EPIKDQNNAI QKGMKENQEN KNAPVSKEGN QKGAKNAPAS KEDNAIKEAP KLSPKEEKRR LKEEKKKAKA EQRAREFEQR AREHQERDEK ELEERR
Validated NT Sequence
tttcttcttt cttcaagctc tttttcatct ctttcttgat gctctctcgc tctttgttca aattctctcg ctctttgttc ggctttggct tttttctttt cttctttcaa gcggcgtttt tcttctttag ggctgagttt tggcgcttct ttaatagcgt tatcctcttt tgaagcagga gcgttttttg cgcctttttg gttaccctct tttgaaacag gagcgttttt gttttcttga ttttctttca tgcctttttg gatagcgtta ttttgatcct taataggctc aggtcttctg gttttaactt cttcttcttc aaacccgcta tttcttggct taactttgtt ttcttctaaa ggcttttctt tagctttatg cctttcttgc aaataaatag gagcgttccc tttttcgcct ttttgagatt ttttagatgc cgatttttcc acaacgggat aaagttttgg cttaaaatcg ttataatcta aatagtagcg atcataatac acccgatttt tatcataaaa caccccttta tccaaacgat tagatgaaaa aaacttaaaa ctgcctatag ttggcgtttt atagacatta tacgaatcaa aatcattcaa atccacctct atcacatacc cgcgcttttc taaagaatac aatttattat ggtttaatac aatggtattg agcgaagcaa agggcagttt cacgctgttt aattccctca aactcttatc catttgcaaa atctgcccgt ctagcgtgag cacatataaa gtccccttat cataaagcaa atccacaata tccccatcat agttgaactc ttgaccgctc actactgaga gtatcctttt ccctgtagaa gcgatcatgt tattgccatc tacaataagg taggtgatat tgttaaaaaa cttatcgctg ctgataacaa tgtttctaat aggcgtaggg tttccatgca cataatccac gactaacaag cgcccatcta gcatggggaa taccacgacc gtatccataa aaataggcat cgccattaaa gaattgatcg tggtgcttgg ggaacctttc tcgctaaaaa gcaatttttg agaagtgatg tcgtataaat tcgctgaatt gtccgctaac accaccgcta aaagattccc tttaacgctc gcgctcaagg caaaagtctc caaagggata acgatagatt gttggctggc gttagggtta ttactaatca attccacttg atgacagact ttatcttgga cttccgcttc aacgcccttt aatttcaatt ccgtttcttc agtcttagcc accttgcttt tgcttgtttt tttatcaatc ttgttcaaac aatcttgcgc aaggataaaa aacccctgac tctcatttaa aaaactgctt tcataattga aattcttacc gattcttaat tgcgttaaac ctttatcgcc tataaccgct ccatttttca aaatggctcc ataacgatta gacgaaacga tactttcttg caaatggtta gggaaatacg cttcgccttt aatttggtgt ttagcgggtt tgaagtattt tctcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMR KYFKPAKHQI KGEAYFPNHL QESIVSSNRY GAILKNGAVI GDKGLTQLRI GKNFNYESSF LNESQGFFIL AQDCLNKIDK KTSKSKVAKT EETELKLKGV EAEVQDKVCH QVELISNNPN ASQQSIVIPL ETFALSASVK GNLLAVVLAD NSANLYDITS QKLLFSEKGS PSTTINSLMA MPIFMDTVVV FPMLDGRLLV VDYVHGNPTP IRNIVISSDK FFNNITYLIV DGNNMIASTG KRILSVVSGQ EFNYDGDIVD LLYDKGTLYV LTLDGQILQM DKSLRELNSV KLPFASLNTI VLNHNKLYSL EKRGYVIEVD LNDFDSYNVY KTPTIGSFKF FSSNRLDKGV FYDKNRVYYD RYYLDYNDFK PKLYPVVEKS ASKKSQKGEK GNAPIYLQER HKAKEKPLEE NKVKPRNSGF EEEEVKTRRP EPIKDQNNAI QKGMKENQEN KNAPVSKEGN QKGAKNAPAS KEDNAIKEAP KLSPKEEKRR LKEEKKKAKA EQRAREFEQR AREHQERDEK ELEERRKALE MNKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAA AATACTTCAA ACCCGCTAAA CACCAAATTA AAGGCGAAGC GTATTTCCCT AACCATTTGC AAGAAAGTAT CGTTTCGTCT AATCGTTATG GAGCCATTTT GAAAAATGGA GCGGTTATAG GCGATAAAGG TTTAACGCAA TTAAGAATCG GTAAGAATTT CAATTATGAA AGCAGTTTTT TAAATGAGAG TCAGGGGTTT TTTATCCTTG CGCAAGATTG TTTGAACAAG ATTGATAAAA AAACAAGCAA AAGCAAGGTG GCTAAGACTG AAGAAACGGA ATTGAAATTA AAGGGCGTTG AAGCGGAAGT CCAAGATAAA GTCTGTCATC AAGTGGAATT GATTAGTAAT AACCCTAACG CCAGCCAACA ATCTATCGTT ATCCCTTTGG AGACTTTTGC CTTGAGCGCG AGCGTTAAAG GGAATCTTTT AGCGGTGGTG TTAGCGGACA ATTCAGCGAA TTTATACGAC ATCACTTCTC AAAAATTGCT TTTTAGCGAG AAAGGTTCCC CAAGCACCAC GATCAATTCT TTAATGGCGA TGCCTATTTT TATGGATACG GTCGTGGTAT TCCCCATGCT AGATGGGCGC TTGTTAGTCG TGGATTATGT GCATGGAAAC CCTACGCCTA TTAGAAACAT TGTTATCAGC AGCGATAAGT TTTTTAACAA TATCACCTAC CTTATTGTAG ATGGCAATAA CATGATCGCT TCTACAGGGA AAAGGATACT CTCAGTAGTG AGCGGTCAAG AGTTCAACTA TGATGGGGAT ATTGTGGATT TGCTTTATGA TAAGGGGACT TTATATGTGC TCACGCTAGA CGGGCAGATT TTGCAAATGG ATAAGAGTTT GAGGGAATTA AACAGCGTGA AACTGCCCTT TGCTTCGCTC AATACCATTG TATTAAACCA TAATAAATTG TATTCTTTAG AAAAGCGCGG GTATGTGATA GAGGTGGATT TGAATGATTT TGATTCGTAT AATGTCTATA AAACGCCAAC TATAGGCAGT TTTAAGTTTT TTTCATCTAA TCGTTTGGAT AAAGGGGTGT TTTATGATAA AAATCGGGTG TATTATGATC GCTACTATTT AGATTATAAC GATTTTAAGC CAAAACTTTA TCCCGTTGTG GAAAAATCGG CATCTAAAAA ATCTCAAAAA GGCGAAAAAG GGAACGCTCC TATTTATTTG CAAGAAAGGC ATAAAGCTAA AGAAAAGCCT TTAGAAGAAA ACAAAGTTAA GCCAAGAAAT AGCGGGTTTG AAGAAGAAGA AGTTAAAACC AGAAGACCTG AGCCTATTAA GGATCAAAAT AACGCTATCC AAAAAGGCAT GAAAGAAAAT CAAGAAAACA AAAACGCTCC TGTTTCAAAA GAGGGTAACC AAAAAGGCGC AAAAAACGCT CCTGCTTCAA AAGAGGATAA CGCTATTAAA GAAGCGCCAA AACTCAGCCC TAAAGAAGAA AAACGCCGCT TGAAAGAAGA AAAGAAAAAA GCCAAAGCCG AACAAAGAGC GAGAGAATTT GAACAAAGAG CGAGAGAGCA TCAAGAAAGA GATGAAAAAG AGCTTGAAGA AAGAAGAAAA GCTTTAGAAA TGAATAAGAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT