HepyC.19480.a
Uncharacterized protein
| CENTER ID: | HepyC.19480.a |
| ORGANISM: | Helicobacter pylori G27 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.19480.a.B11.GE41744 | full length | 1 | 347 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.19480.a.B11.PW38477 | full length | 1 | 347 |
Structures
6MTZ
DEPOSITED: 10/22/2018
DETERMINATION: XRay
CLONE: HepyC.19480.a.B11.GE41744
PROTEIN: HepyC.19480.a.B11.PW38477
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|563041.6.peg.421 |
| UniProt: | B5Z6F2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVYHLSHID LDGYACQLVS KQFFKNIQCY NANYGREVSA RIYEILNAIA QSKESEFLIL VSDLNLNLNE AEYLQDKIQE HRLQNKNIQI QLLDHHISGK EVAESFHWYF LDTNRCATKI VYEFLKKHYA ILEPKNTTWL EPLVEMVNSV DIWDTQGYGF ELGKVCMRMI TQSSELNRFM FDDENRDYKL KLLEEVKNYL FLENAPVAYD NDLFRLKKIA LGGDPDTETM DNISSNAQTH LLSLKKHDCS VYYQDKKGFL SYSMGGISVL ANLFLTQNPD FDFYIDVNAK GNVSLRANGN CDVCELSQMC FNGGGHRNAS GGKIDGFRES FNYRDIKEQI EEIFNNA
NT Sequence
atgcaagttt accacctttc acacattgat ttagacggct atgcatgcca gcttgtttca aaacaatttt ttaaaaatat ccaatgctat aacgctaatt atgggcgtga agtctcagcg agaatttatg aaattctaaa cgcaatcgct caatctaaag agagtgaatt ccttattttg gttagcgatt tgaatttgaa tttaaacgaa gcagagtatt tgcaagataa aatccaagaa caccgcttgc aaaataaaaa cattcaaatc cagcttttag atcaccatat cagcggtaag gaagtggctg agagtttcca ttggtatttt ttagacacga accgctgcgc gactaaaatc gtgtatgaat ttttgaaaaa gcattacgct attttagagc caaaaaacac aacatggcta gagcctttag tggaaatggt caattctgtg gatatttggg acacgcaagg ttatggcttt gaattaggca aggtgtgcat gcgcatgatc actcaaagct ctgaattgaa tcgtttcatg tttgatgatg aaaaccgcga ttataaatta aagcttttag aagaagttaa aaactatttg tttttagaaa atgcccctgt agcctatgat aatgatttgt tcaggcttaa aaaaatcgct ttagggggcg accctgatac agaaacgatg gacaatattt cttcaaacgc gcaaacgcat ttgctctctt taaaaaagca tgattgcagc gtttattacc aagataaaaa agggttttta agttattcta tggggggtat tagcgtgttg gctaaccttt ttttaacgca aaatccggat tttgattttt atatagatgt gaacgctaaa gggaatgtga gcttaagagc gaatgggaat tgcgatgtgt gcgaactcag tcaaatgtgt tttaatgggg gcgggcatag gaatgcgagc ggaggcaaga ttgatggctt tagagagagt ttcaattata gggatattaa agaacaaatt gaagaaatct tcaataacgc ttaa
Details for HepyC.19480.a.B11.GE41744
| HARVESTED ON: | 6/19/2017 |
| SEQUENCED ON: | 6/22/2017 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMQ VYHLSHIDLD GYACQLVSKQ FFKNIQCYNA NYGREVSARI YEILNAIAQS KESEFLILVS DLNLNLNEAE YLQDKIQEHR LQNKNIQIQL LDHHISGKEV AESFHWYFLD TNRCATKIVY EFLKKHYAIL EPKNTTWLEP LVEMVNSVDI WDTQGYGFEL GKVCMRMITQ SSELNRFMFD DENRDYKLKL LEEVKNYLFL ENAPVAYDND LFRLKKIALG GDPDTETMDN ISSNAQTHLL SLKKHDCSVY YQDKKGFLSY SMGGISVLAN LFLTQNPDFD FYIDVNAKGN VSLRANGNCD VCELSQMCFN GGGHRNASGG KIDGFRESFN YRDIKEQ
Validated NT Sequence
aatttgttct ttaatatccc tataattgaa actctctcta aagccatcaa tcttgcctcc gctcgcattc ctatgcccgc ccccattaaa acacatttga ctgagttcgc acacatcgca attcccattc gctcttaagc tcacattccc tttagcgttc acatctatat aaaaatcaaa atccggattt tgcgttaaaa aaaggttagc caacacgcta atacccccca tagaataact taaaaaccct tttttatctt ggtaataaac gctgcaatca tgctttttta aagagagcaa atgcgtttgc gcgtttgaag aaatattgtc catcgtttct gtatcagggt cgccccctaa agcgattttt ttaagcctga acaaatcatt atcataggct acaggggcat tttctaaaaa caaatagttt ttaacttctt ctaaaagctt taatttataa tcgcggtttt catcatcaaa catgaaacga ttcaattcag agctttgagt gatcatgcgc atgcacacct tgcctaattc aaagccataa ccttgcgtgt cccaaatatc cacagaattg accatttcca ctaaaggctc tagccatgtt gtgttttttg gctctaaaat agcgtaatgc tttttcaaaa attcatacac gattttagtc gcgcagcggt tcgtgtctaa aaaataccaa tggaaactct cagccacttc cttaccgctg atatggtgat ctaaaagctg gatttgaatg tttttatttt gcaagcggtg ttcttggatt ttatcttgca aatactctgc ttcgtttaaa ttcaaattca aatcgctaac caaaataagg aattcactct ctttagattg agcgattgcg tttagaattt cataaattct cgctgagact tcacgcccat aattagcgtt atagcattgg atatttttaa aaaattgttt tgaaacaagc tggcatgcat agccgtctaa atcaatgtgt gaaaggtggt aaacttgcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMQ VYHLSHIDLD GYACQLVSKQ FFKNIQCYNA NYGREVSARI YEILNAIAQS KESEFLILVS DLNLNLNEAE YLQDKIQEHR LQNKNIQIQL LDHHISGKEV AESFHWYFLD TNRCATKIVY EFLKKHYAIL EPKNTTWLEP LVEMVNSVDI WDTQGYGFEL GKVCMRMITQ SSELNRFMFD DENRDYKLKL LEEVKNYLFL ENAPVAYDND LFRLKKIALG GDPDTETMDN ISSNAQTHLL SLKKHDCSVY YQDKKGFLSY SMGGISVLAN LFLTQNPDFD FYIDVNAKGN VSLRANGNCD VCELSQMCFN GGGHRNASGG KIDGFRESFN YRDIKEQIEE IFNNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag tttaccacct ttcacacatt gatttagacg gctatgcatg ccagcttgtt tcaaaacaat tttttaaaaa tatccaatgc tataacgcta attatgggcg tgaagtctca gcgagaattt atgaaattct aaacgcaatc gctcaatcta aagagagtga attccttatt ttggttagcg atttgaattt gaatttaaac gaagcagagt atttgcaaga taaaatccaa gaacaccgct tgcaaaataa aaacattcaa atccagcttt tagatcacca tatcagcggt aaggaagtgg ctgagagttt ccattggtat tttttagaca cgaaccgctg cgcgactaaa atcgtgtatg aatttttgaa aaagcattac gctattttag agccaaaaaa cacaacatgg ctagagcctt tagtggaaat ggtcaattct gtggatattt gggacacgca aggttatggc tttgaattag gcaaggtgtg catgcgcatg atcactcaaa gctctgaatt gaatcgtttc atgtttgatg atgaaaaccg cgattataaa ttaaagcttt tagaagaagt taaaaactat ttgtttttag aaaatgcccc tgtagcctat gataatgatt tgttcaggct taaaaaaatc gctttagggg gcgaccctga tacagaaacg atggacaata tttcttcaaa cgcgcaaacg catttgctct ctttaaaaaa gcatgattgc agcgtttatt accaagataa aaaagggttt ttaagttatt ctatgggggg tattagcgtg ttggctaacc tttttttaac gcaaaatccg gattttgatt tttatataga tgtgaacgct aaagggaatg tgagcttaag agcgaatggg aattgcgatg tgtgcgaact cagtcaaatg tgttttaatg ggggcgggca taggaatgcg agcggaggca agattgatgg ctttagagag agtttcaatt atagggatat taaagaacaa attgaagaaa tcttcaataa cgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for HepyC.19480.a.B11.PW38477
| PURIFICATION DATe: | 5/21/2018 |
| CONCENTRATION: | 31.62mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 34 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ VYHLSHIDLD GYACQLVSKQ FFKNIQCYNA NYGREVSARI YEILNAIAQS KESEFLILVS DLNLNLNEAE YLQDKIQEHR LQNKNIQIQL LDHHISGKEV AESFHWYFLD TNRCATKIVY EFLKKHYAIL EPKNTTWLEP LVEMVNSVDI WDTQGYGFEL GKVCMRMITQ SSELNRFMFD DENRDYKLKL LEEVKNYLFL ENAPVAYDND LFRLKKIALG GDPDTETMDN ISSNAQTHLL SLKKHDCSVY YQDKKGFLSY SMGGISVLAN LFLTQNPDFD FYIDVNAKGN VSLRANGNCD VCELSQMCFN GGGHRNASGG KIDGFRESFN YRDIKEQ
Validated NT Sequence
aatttgttct ttaatatccc tataattgaa actctctcta aagccatcaa tcttgcctcc gctcgcattc ctatgcccgc ccccattaaa acacatttga ctgagttcgc acacatcgca attcccattc gctcttaagc tcacattccc tttagcgttc acatctatat aaaaatcaaa atccggattt tgcgttaaaa aaaggttagc caacacgcta atacccccca tagaataact taaaaaccct tttttatctt ggtaataaac gctgcaatca tgctttttta aagagagcaa atgcgtttgc gcgtttgaag aaatattgtc catcgtttct gtatcagggt cgccccctaa agcgattttt ttaagcctga acaaatcatt atcataggct acaggggcat tttctaaaaa caaatagttt ttaacttctt ctaaaagctt taatttataa tcgcggtttt catcatcaaa catgaaacga ttcaattcag agctttgagt gatcatgcgc atgcacacct tgcctaattc aaagccataa ccttgcgtgt cccaaatatc cacagaattg accatttcca ctaaaggctc tagccatgtt gtgttttttg gctctaaaat agcgtaatgc tttttcaaaa attcatacac gattttagtc gcgcagcggt tcgtgtctaa aaaataccaa tggaaactct cagccacttc cttaccgctg atatggtgat ctaaaagctg gatttgaatg tttttatttt gcaagcggtg ttcttggatt ttatcttgca aatactctgc ttcgtttaaa ttcaaattca aatcgctaac caaaataagg aattcactct ctttagattg agcgattgcg tttagaattt cataaattct cgctgagact tcacgcccat aattagcgtt atagcattgg atatttttaa aaaattgttt tgaaacaagc tggcatgcat agccgtctaa atcaatgtgt gaaaggtggt aaacttgcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMQ VYHLSHIDLD GYACQLVSKQ FFKNIQCYNA NYGREVSARI YEILNAIAQS KESEFLILVS DLNLNLNEAE YLQDKIQEHR LQNKNIQIQL LDHHISGKEV AESFHWYFLD TNRCATKIVY EFLKKHYAIL EPKNTTWLEP LVEMVNSVDI WDTQGYGFEL GKVCMRMITQ SSELNRFMFD DENRDYKLKL LEEVKNYLFL ENAPVAYDND LFRLKKIALG GDPDTETMDN ISSNAQTHLL SLKKHDCSVY YQDKKGFLSY SMGGISVLAN LFLTQNPDFD FYIDVNAKGN VSLRANGNCD VCELSQMCFN GGGHRNASGG KIDGFRESFN YRDIKEQIEE IFNNA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAG TTTACCACCT TTCACACATT GATTTAGACG GCTATGCATG CCAGCTTGTT TCAAAACAAT TTTTTAAAAA TATCCAATGC TATAACGCTA ATTATGGGCG TGAAGTCTCA GCGAGAATTT ATGAAATTCT AAACGCAATC GCTCAATCTA AAGAGAGTGA ATTCCTTATT TTGGTTAGCG ATTTGAATTT GAATTTAAAC GAAGCAGAGT ATTTGCAAGA TAAAATCCAA GAACACCGCT TGCAAAATAA AAACATTCAA ATCCAGCTTT TAGATCACCA TATCAGCGGT AAGGAAGTGG CTGAGAGTTT CCATTGGTAT TTTTTAGACA CGAACCGCTG CGCGACTAAA ATCGTGTATG AATTTTTGAA AAAGCATTAC GCTATTTTAG AGCCAAAAAA CACAACATGG CTAGAGCCTT TAGTGGAAAT GGTCAATTCT GTGGATATTT GGGACACGCA AGGTTATGGC TTTGAATTAG GCAAGGTGTG CATGCGCATG ATCACTCAAA GCTCTGAATT GAATCGTTTC ATGTTTGATG ATGAAAACCG CGATTATAAA TTAAAGCTTT TAGAAGAAGT TAAAAACTAT TTGTTTTTAG AAAATGCCCC TGTAGCCTAT GATAATGATT TGTTCAGGCT TAAAAAAATC GCTTTAGGGG GCGACCCTGA TACAGAAACG ATGGACAATA TTTCTTCAAA CGCGCAAACG CATTTGCTCT CTTTAAAAAA GCATGATTGC AGCGTTTATT ACCAAGATAA AAAAGGGTTT TTAAGTTATT CTATGGGGGG TATTAGCGTG TTGGCTAACC TTTTTTTAAC GCAAAATCCG GATTTTGATT TTTATATAGA TGTGAACGCT AAAGGGAATG TGAGCTTAAG AGCGAATGGG AATTGCGATG TGTGCGAACT CAGTCAAATG TGTTTTAATG GGGGCGGGCA TAGGAATGCG AGCGGAGGCA AGATTGATGG CTTTAGAGAG AGTTTCAATT ATAGGGATAT TAAAGAACAA ATTGAAGAAA TCTTCAATAA CGCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT