HepyC.19478.a

Outer membrane protein

CENTER ID: HepyC.19478.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19478.a.B21.GE41742 mature protein 19 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.643
UniProt: B5Z710

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKALLLTLS LSFWLHAERN GFYLGLNFLE GSYIKGQGSI GKKASAENAL NEAINNAKNS LFPEQNTKAI RDAQNALNAV KDSNKIANRF AGNGGSGGLF NELSFGYKYF LGKKRIIGFR HSLFFGYQLG GVGSVPGSGL IAFLPYGFNT DLLINWTNDK RASQKYVERR VKGLSIFYKD MTGRTLDANT LKKASRHVFR KSSGLVIGME LGGSTWFASN NLTPFNQVKS RTIFQLQGKF GVRWNNDEYD IDRYGNEIYL GGSSVELGVK VPAFKVNYYS DDYGDKLDYK RVVSVYLNYT YNFKNKH
NT Sequence
atgaaaaaag ctctcttact aactctctct ctctcgttct ggctccacgc tgaaaggaac gggttttatt taggtttaaa ttttctagaa ggaagctaca ttaaaggaca aggtagcatc ggcaaaaaag cttcagcaga aaacgcctta aatgaagcga tcaataacgc aaaaaattca ttattccccg aacaaaacac aaaagccata agagatgcgc aaaacgcctt aaatgcagtg aaagattcaa acaaaatcgc taaccgattc gcaggaaatg gtggatcggg cggtcttttt aatgagctca gctttgggta taaatatttt ttaggtaaaa aaaggattat agggtttagg cactctcttt ttttcggtta ccaacttggt ggcgttggtt ctgttcctgg cagcggttta atagcttttt taccctatgg tttcaatacg gatttgctca ttaattggac taacgataag cgagcgtccc aaaaatatgt tgaacgaagg gtaaaagggc tttctatatt ttacaaagat atgaccggca gaacgctaga cgctaataca ttaaaaaaag catcaaggca tgtatttaga aaatcttcag ggcttgtgat tggcatggaa ctagggggta gcacttggtt tgcaagtaac aatctcaccc ctttcaatca agtcaagagt cgcacgattt ttcagttgca aggaaaattt ggcgttcgtt ggaataatga tgaatacgat attgatcgct atggcaatga aatctatctt ggaggttcta gcgtggaatt aggggttaaa gtgccagcgt ttaaagtcaa ttactatagc gatgattatg gggataaatt ggattataaa agagtggtga gcgtttatct taactataca tataacttta aaaacaaaca ttaa
Details for HepyC.19478.a.B21.GE41742
HARVESTED ON: 6/19/2017
SEQUENCED ON: 6/22/2017
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR NGFYLGLNFL EGSYIKGQGS IGKKASAENA LNEAINNAKN SLFPEQNTKA IRDAQNALNA VKDSNKIANR FAGNGGSGGL FNELSFGYKY FLGKKRIIGF RHSLFFGYQL GGVGSVPGSG LIAFLPYGFN TDLLINWTND KRASQKYVER RVKGLSIFYK DMTGRTLDAN TLKKASRHVF RKSSGLVIGM ELGGSTWFAS NNLTPFNQVK SRTIFQLQGK FGVRWNNDEY DIDRYGNEIY LGGSSVELGV KVPAFKVNYY SDDYGDKLDY KRVVSVYLNY TYNFKNKH
Validated NT Sequence
ttaatgtttg tttttaaagt tatatgtata gttaagataa acgctcacca ctcttttata atccaattta tccccataat catcgctata gtaattgact ttaaacgctg gcactttaac ccctaattcc acgctagaac ctccaagata gatttcattg ccatagcgat caatatcgta ttcatcatta ttccaacgaa cgccaaattt tccttgcaac tgaaaaatcg tgcgactctt gacttgattg aaaggggtga gattgttact tgcaaaccaa gtgctacccc ctagttccat gccaatcaca agccctgaag attttctaaa tacatgcctt gatgcttttt ttaatgtatt agcgtctagc gttctgccgg tcatatcttt gtaaaatata gaaagccctt ttacccttcg ttcaacatat ttttgggacg ctcgcttatc gttagtccaa ttaatgagca aatccgtatt gaaaccatag ggtaaaaaag ctattaaacc gctgccagga acagaaccaa cgccaccaag ttggtaaccg aaaaaaagag agtgcctaaa ccctataatc ctttttttac ctaaaaaata tttataccca aagctgagct cattaaaaag accgcccgat ccaccatttc ctgcgaatcg gttagcgatt ttgtttgaat ctttcactgc atttaaggcg ttttgcgcat ctcttatggc ttttgtgttt tgttcgggga ataatgaatt ttttgcgtta ttgatcgctt catttaaggc gttttctgct gaagcttttt tgccgatgct accttgtcct ttaatgtagc ttccttctag aaaatttaaa cctaaataaa acccgttcct catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHRN GFYLGLNFLE GSYIKGQGSI GKKASAENAL NEAINNAKNS LFPEQNTKAI RDAQNALNAV KDSNKIANRF AGNGGSGGLF NELSFGYKYF LGKKRIIGFR HSLFFGYQLG GVGSVPGSGL IAFLPYGFNT DLLINWTNDK RASQKYVERR VKGLSIFYKD MTGRTLDANT LKKASRHVFR KSSGLVIGME LGGSTWFASN NLTPFNQVKS RTIFQLQGKF GVRWNNDEYD IDRYGNEIYL GGSSVELGVK VPAFKVNYYS DDYGDKLDYK RVVSVYLNYT YNFKNKH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataggaacg ggttttattt aggtttaaat tttctagaag gaagctacat taaaggacaa ggtagcatcg gcaaaaaagc ttcagcagaa aacgccttaa atgaagcgat caataacgca aaaaattcat tattccccga acaaaacaca aaagccataa gagatgcgca aaacgcctta aatgcagtga aagattcaaa caaaatcgct aaccgattcg caggaaatgg tggatcgggc ggtcttttta atgagctcag ctttgggtat aaatattttt taggtaaaaa aaggattata gggtttaggc actctctttt tttcggttac caacttggtg gcgttggttc tgttcctggc agcggtttaa tagctttttt accctatggt ttcaatacgg atttgctcat taattggact aacgataagc gagcgtccca aaaatatgtt gaacgaaggg taaaagggct ttctatattt tacaaagata tgaccggcag aacgctagac gctaatacat taaaaaaagc atcaaggcat gtatttagaa aatcttcagg gcttgtgatt ggcatggaac tagggggtag cacttggttt gcaagtaaca atctcacccc tttcaatcaa gtcaagagtc gcacgatttt tcagttgcaa ggaaaatttg gcgttcgttg gaataatgat gaatacgata ttgatcgcta tggcaatgaa atctatcttg gaggttctag cgtggaatta ggggttaaag tgccagcgtt taaagtcaat tactatagcg atgattatgg ggataaattg gattataaaa gagtggtgag cgtttatctt aactatacat ataactttaa aaacaaacat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat