HepyC.19477.a

Rod shape-determining protein

CENTER ID: HepyC.19477.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19477.a.B21.GE41741 mature protein 27 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1425
UniProt: B5Z916

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFYFKFLWL LGIFLIFYFL DIKGSSSYIS DRVKNALMSA KNSLLDNIQA YFFQAKNIKE FQKERLILEA LKLENADLKE RLNSVYPLEN PKMIYSPTFM TSFISLEDTH SVSLNPIVNL EENKIYGLVS HNQAIGIAVL EKGRLNGFLN AHKRCAYSVM IGKNQVLGFI GSNFKQELVV DFIVPSAEIN IGDQVLTSGL DGIFGAGVFV GEVSSVEDHY TYKSAVLKNA FLSEAKLLRH VFLSDVKN
NT Sequence
atgcgttttt attttaaatt cctttggctt ttagggattt ttcttatttt ttatttttta gacattaaag gcagctcttc ttatatcagc gatagggtta aaaacgcctt gatgagtgcg aaaaacagct tactagacaa cattcaagcg tatttttttc aagccaaaaa cattaaggaa tttcaaaaag aacgcttgat tttagaagct ttaaaactag aaaacgctga tttgaaagag cgtttgaaca gcgtttatcc tttagaaaat ccaaaaatga tctattcccc cacttttatg acttcattca tcagtttaga agacacgcac agcgtctctc tcaatcctat tgtgaattta gaagaaaata agatttatgg ccttgtctct cacaatcaag ccataggcat tgcggtgcta gaaaaagggc gcttgaacgg gtttttgaac gcccacaagc ggtgcgctta tagcgtgatg ataggcaaaa atcaagtgct aggctttata gggtctaatt tcaagcaaga attagtcgtg gattttattg tccctagcgc tgaaattaac ataggcgatc aggtgctaac gagcgggcta gatgggattt ttggagcggg ggtgtttgtg ggcgaagttt caagcgttga agatcattac acttataaaa gcgcggtgtt gaaaaacgct tttttaagcg aagccaaact tttaaggcat gtgtttttaa gcgatgtgaa aaactag
Details for HepyC.19477.a.B21.GE41741
HARVESTED ON: 6/19/2017
SEQUENCED ON: 6/22/2017
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS YISDRVKNAL MSAKNSLLDN IQAYFFQAKN IKEFQKERLI LEALKLENAD LKERLNSVYP LENPKMIYSP TFMTSFISLE DTHSVSLNPI VNLEENKIYG LVSHNQAIGI AVLEKGRLNG FLNAHKRCAY SVMIGKNQVL GFIGSNFKQE LVVDFIVPSA EINIGDQVLT SGLDGIFGAG VFVGEVSSVE DHYTYKSAVL KNAFLSEAKL LRHV
Validated NT Sequence
acacatgcct taaaagtttg gcttcgctta aaaaagcgtt tttcaacacc gcgcttttat aagtgtaatg atcttcaacg cttgaaactt cgcccacaaa cacccccgct ccaaaaatcc catctagccc gctcgttagc acctgatcgc ctatgttaat ttcagcgcta gggacaataa aatccacgac taattcttgc ttgaaattag accctataaa gcctagcact tgatttttgc ctatcatcac gctataagcg caccgcttgt gggcgttcaa aaacccgttc aagcgccctt tttctagcac cgcaatgcct atggcttgat tgtgagagac aaggccataa atcttatttt cttctaaatt cacaatagga ttgagagaga cgctgtgcgt gtcttctaaa ctgatgaatg aagtcataaa agtgggggaa tagatcattt ttggattttc taaaggataa acgctgttca aacgctcttt caaatcagcg ttttctagtt ttaaagcttc taaaatcaag cgttcttttt gaaattcctt aatgtttttg gcttgaaaaa aatacgcttg aatgttgtct agtaagctgt ttttcgcact catcaaggcg tttttaaccc tatcgctgat ataagacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHSY ISDRVKNALM SAKNSLLDNI QAYFFQAKNI KEFQKERLIL EALKLENADL KERLNSVYPL ENPKMIYSPT FMTSFISLED THSVSLNPIV NLEENKIYGL VSHNQAIGIA VLEKGRLNGF LNAHKRCAYS VMIGKNQVLG FIGSNFKQEL VVDFIVPSAE INIGDQVLTS GLDGIFGAGV FVGEVSSVED HYTYKSAVLK NAFLSEAKLL RHVFLSDVKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcttata tcagcgatag ggttaaaaac gccttgatga gtgcgaaaaa cagcttacta gacaacattc aagcgtattt ttttcaagcc aaaaacatta aggaatttca aaaagaacgc ttgattttag aagctttaaa actagaaaac gctgatttga aagagcgttt gaacagcgtt tatcctttag aaaatccaaa aatgatctat tcccccactt ttatgacttc attcatcagt ttagaagaca cgcacagcgt ctctctcaat cctattgtga atttagaaga aaataagatt tatggccttg tctctcacaa tcaagccata ggcattgcgg tgctagaaaa agggcgcttg aacgggtttt tgaacgccca caagcggtgc gcttatagcg tgatgatagg caaaaatcaa gtgctaggct ttatagggtc taatttcaag caagaattag tcgtggattt tattgtccct agcgctgaaa ttaacatagg cgatcaggtg ctaacgagcg ggctagatgg gatttttgga gcgggggtgt ttgtgggcga agtttcaagc gttgaagatc attacactta taaaagcgcg gtgttgaaaa acgctttttt aagcgaagcc aaacttttaa ggcatgtgtt tttaagcgat gtgaaaaact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat