SSGCID: Target Page

HepyC.19475.a

Pyruvate ferredoxin oxidoreductase, alpha subunit

CENTER ID:	HepyC.19475.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.19475.a.B11.GE41739	full length		1	407

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.19475.a.B11.PS38441	full length		1	407

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1145
UniProt:	B5Z8A6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARSIELQEI EVWDGNTASS NALRQAQIDV IAAYPITPST PIVQSYGSFK DNGYVDGEFV LVESEHAAMS ACVGAAAAGG RVSTATSSQG LALMVEVLYQ ASGMRLPIVL NLVNRALAAP LNIHGDHSDM YLSRDSGWIS LCTCNPQEAY DFTLMAFKIA EHQKVRVPTI VNQDGFLCSH TVQNVRPLSD AVAYQFVGEY QTKHSLLDFD KPVSYGAQAE EEWHYEHKAQ LHHAIMSASS VIEEVFNDFA KLTGRQYHLT KTFQLEDAEI AIFALGTTYE SAIVAAKEMR KKGIKAGVAT IHSLRPFPYE RLGQDLKNLK ALAILDKSSP AGAMGAMFNE VTSAVYQTQG TKHPVVSNYI YGLGERDMTI VHLCEIFEEI NEDALKGTLT HPTQQFVGLH GPKMSFF

NT Sequence

atggcaagaa gtattgaatt gcaagagata gaagtgtggg atggcaatac cgctagctct aacgctttaa gacaggctca aattgatgtc atcgcagcct atcctatcac cccatcaacg cctattgtgc agagttatgg ctcgtttaag gataatggct atgttgatgg cgaattcgtt ttagtggaat ctgagcatgc cgccatgagc gcatgcgtgg gagctgcagc tgccggtggt agagtcagca ctgcgaccag ctctcaaggt ttggcgttaa tggtagaggt tttataccaa gcttctggca tgcgtttgcc tattgttttg aatttagtca atcgcgctct agcagcccct ttaaatattc atggcgatca ttctgatatg tatttaagca gggattcagg ctggatcagt ttatgcacat gcaaccccca agaagcttat gatttcactt taatggcgtt taaaatcgca gagcatcaaa aggtgcgcgt gcctactatt gtcaatcaag acgggttttt atgctcgcac accgtgcaaa atgtccgccc tttgagcgat gcagtggctt atcaattcgt gggcgaatac caaaccaagc attccctttt ggattttgat aaaccggtaa gctatggtgc gcaagctgaa gaagaatggc attatgagca taaagcccaa ctccaccatg ccatcatgag tgcgtcttct gtgattgaag aagtgttcaa tgattttgct aaactcacag gcaggcaata ccatttgact aaaactttcc agctagaaga cgctgaaatc gctatctttg cgttaggcac tacttatgaa tcagcgatcg tagcggctaa agaaatgcgt aaaaaaggca ttaaggccgg cgtggccact atccattcct tacgcccctt cccttatgaa agattagggc aggatttgaa aaaccttaaa gctttagcga ttttagacaa gagctctcca gcgggcgcta tgggggcgat gtttaatgaa gtaacgagcg cggtgtatca aacgcaaggg actaaacacc ccgtggtgtc taactacatt tatggtttag gcgaaaggga tatgacgatc gtgcatttat gcgagatttt tgaagaaatc aatgaagacg ctcttaaagg cacgctcacg caccctaccc aacaattcgt aggtttgcac ggccctaaaa tgagcttttt ttaa

Details for HepyC.19475.a.B11.GE41739

HARVESTED ON:	6/19/2017
SEQUENCED ON:	6/22/2017
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA RSIELQEIEV WDGNTASSNA LRQAQIDVIA AYPITPSTPI VQSYGSFKDN GYVDGEFVLV ESEHAAMSAC VGAAAAGGRV STATSSQGLA LMVEVLYQAS GMRLPIVLNL VNRALAAPLN IHGDHSDMYL SRDSGWISLC TCNPQEAYDF TLMAFKIAEH QKVRVPTIVN QDGFLCSHTV QNVRPLSDAV AYQFVGEYQT KHSLLDFDKP VSYGAQAEEE WHYEHKAQLH HAIMSASSVI EEVFNDFAKL TGRQYHLTKT FQLEDAEIAI FALGTTYESA IVAAKEMRKK GIKAGVATIH SLRPFPYERL GQDLKNLKAL AILDKSSPAG AMGAMFNEVT SAVYQTQGTK HPVVSNYIYG LGERDMTIVH LCEIFEEINE DALKGTLTHP TQQFVGLHGP KMSFF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa gaagtattga attgcaagag atagaagtgt gggatggcaa taccgctagc tctaacgctt taagacaggc tcaaattgat gtcatcgcag cctatcctat caccccatca acgcctattg tgcagagtta tggctcgttt aaggataatg gctatgttga tggcgaattc gttttagtgg aatctgagca tgccgccatg agcgcatgcg tgggagctgc agctgccggt ggtagagtca gcactgcgac cagctctcaa ggtttggcgt taatggtaga ggttttatac caagcttctg gcatgcgttt gcctattgtt ttgaatttag tcaatcgcgc tctagcagcc cctttaaata ttcatggcga tcattctgat atgtatttaa gcagggattc aggctggatc agtttatgca catgcaaccc ccaagaagct tatgatttca ctttaatggc gtttaaaatc gcagagcatc aaaaggtgcg cgtgcctact attgtcaatc aagacgggtt tttatgctcg cacaccgtgc aaaatgtccg ccctttgagc gatgcagtgg cttatcaatt cgtgggcgaa taccaaacca agcattccct tttggatttt gataaaccgg taagctatgg tgcgcaagct gaagaagaat ggcattatga gcataaagcc caactccacc atgccatcat gagtgcgtct tctgtgattg aagaagtgtt caatgatttt gctaaactca caggcaggca ataccatttg actaaaactt tccagctaga agacgctgaa atcgctatct ttgcgttagg cactacttat gaatcagcga tcgtagcggc taaagaaatg cgtaaaaaag gcattaaggc cggcgtggcc actatccatt ccttacgccc cttcccttat gaaagattag ggcaggattt gaaaaacctt aaagctttag cgattttaga caagagctct ccagcgggcg ctatgggggc gatgtttaat gaagtaacga gcgcggtgta tcaaacgcaa gggactaaac accccgtggt gtctaactac atttatggtt taggcgaaag ggatatgacg atcgtgcatt tatgcgagat ttttgaagaa atcaatgaag acgctcttaa aggcacgctc acgcacccta cccaacaatt cgtaggtttg cacggcccta aaatgagctt tttttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for HepyC.19475.a.B11.PS38441

PURIFICATION DATe:	5/15/2018
CONCENTRATION:	11.92mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA GAAGTATTGA ATTGCAAGAG ATAGAAGTGT GGGATGGCAA TACCGCTAGC TCTAACGCTT TAAGACAGGC TCAAATTGAT GTCATCGCAG CCTATCCTAT CACCCCATCA ACGCCTATTG TGCAGAGTTA TGGCTCGTTT AAGGATAATG GCTATGTTGA TGGCGAATTC GTTTTAGTGG AATCTGAGCA TGCCGCCATG AGCGCATGCG TGGGAGCTGC AGCTGCCGGT GGTAGAGTCA GCACTGCGAC CAGCTCTCAA GGTTTGGCGT TAATGGTAGA GGTTTTATAC CAAGCTTCTG GCATGCGTTT GCCTATTGTT TTGAATTTAG TCAATCGCGC TCTAGCAGCC CCTTTAAATA TTCATGGCGA TCATTCTGAT ATGTATTTAA GCAGGGATTC AGGCTGGATC AGTTTATGCA CATGCAACCC CCAAGAAGCT TATGATTTCA CTTTAATGGC GTTTAAAATC GCAGAGCATC AAAAGGTGCG CGTGCCTACT ATTGTCAATC AAGACGGGTT TTTATGCTCG CACACCGTGC AAAATGTCCG CCCTTTGAGC GATGCAGTGG CTTATCAATT CGTGGGCGAA TACCAAACCA AGCATTCCCT TTTGGATTTT GATAAACCGG TAAGCTATGG TGCGCAAGCT GAAGAAGAAT GGCATTATGA GCATAAAGCC CAACTCCACC ATGCCATCAT GAGTGCGTCT TCTGTGATTG AAGAAGTGTT CAATGATTTT GCTAAACTCA CAGGCAGGCA ATACCATTTG ACTAAAACTT TCCAGCTAGA AGACGCTGAA ATCGCTATCT TTGCGTTAGG CACTACTTAT GAATCAGCGA TCGTAGCGGC TAAAGAAATG CGTAAAAAAG GCATTAAGGC CGGCGTGGCC ACTATCCATT CCTTACGCCC CTTCCCTTAT GAAAGATTAG GGCAGGATTT GAAAAACCTT AAAGCTTTAG CGATTTTAGA CAAGAGCTCT CCAGCGGGCG CTATGGGGGC GATGTTTAAT GAAGTAACGA GCGCGGTGTA TCAAACGCAA GGGACTAAAC ACCCCGTGGT GTCTAACTAC ATTTATGGTT TAGGCGAAAG GGATATGACG ATCGTGCATT TATGCGAGAT TTTTGAAGAA ATCAATGAAG ACGCTCTTAA AGGCACGCTC ACGCACCCTA CCCAACAATT CGTAGGTTTG CACGGCCCTA AAATGAGCTT TTTTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT