SSGCID: Target Page

HepyC.19473.a

Magnesium transport protein CorA

CENTER ID:	HepyC.19473.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.19473.a.B21.GE41737	soluble domain		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1399
UniProt:	B5Z8Z1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVNVFFKQQK FVIKKRFNDF NGFDIEENEV LWFELINPTP NELATLSQEY AIHYNTDHSQ RVSSVTKYWE DSSSVTINAF FTNQDENETF HTEMATFILS NNILFTIYYG TLEIFDSIQK KVLASPKKFE DGFDILTKIF EVYFEKGVEC LEWINKQTSL LRKNIIFKET STHDDILVHL SNLQEFNVAL RDSFFDKRRI ITALLRSNKV DSDTKNNLNI ILTDFSSLVE STTVNLNSLD NIQNLFASQV NVEQNKIIKL FTVATMAMMP PTLIGTIYGM NFKFMPELEW QYGYLFALIV MAISTILPVL YFKKKGWL

NT Sequence

atggtgaacg tgtttttcaa acagcaaaaa tttgtcatta aaaaacgctt taatgatttt aatggctttg atatagaaga aaatgaagtt ttatggtttg aattaatcaa ccccacgccc aatgaattag ccactctaag ccaagaatac gctatccact acaacacaga tcattcccaa cgagtctcat cagttaccaa atattgggaa gacagctcca gcgttacgat caacgccttt ttcactaacc aggatgaaaa tgagactttc catacggaaa tggcgacctt tattttgtct aataacattc ttttcacgat ttattacggg actttagaaa tctttgattc tatccaaaaa aaggttttgg ctagccctaa aaaatttgaa gacgggtttg atattctaac caaaatcttt gaagtatatt ttgaaaaagg ggtggaatgt ttggagtgga tcaacaaaca aacgagcctg ttgcgtaaaa acatcatttt caaagaaact tctacgcatg atgatatttt agtgcacttg tccaatttgc aagaatttaa tgtggcttta agggattcct tttttgacaa acggcgcatt atcaccgctt tattaaggag caataaagtg gatagcgata ccaaaaataa tttaaatatc attttaaccg attttagctc tttggtggag tctacaacgg tcaatctcaa ctcgcttgat aacattcaaa acctgttcgc ttctcaagtc aatgtggagc aaaataaaat catcaagctc ttcacggtgg cgaccatggc gatgatgccc cccactttga ttggcacgat ttatggcatg aattttaaat tcatgccgga gttagaatgg caatacgggt atcttttcgc gctgattgtc atggcgattt ctacgatttt gcctgtgctc tattttaaaa agaaaggctg gttgtag

Details for HepyC.19473.a.B21.GE41737

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/22/2017
EXPECTED MW:	31kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

ttactcactt acttgatgat tttattttnn nnnnnnnnnn nttgagaagc gaacaggttt tgaatgttat caagcgagtt gagattgacc gttgtagact ccaccaaaga gctaaaatcg gttaaaatga tatttaaatt atttttggta tcgctatcca ctttattgct ccttaataaa gcggtgataa tgcgccgttt gtcaaaaaag gaatccctta aagccacatt aaattcttgc aaattggaca agtgcactaa aatatcatca tgcgtagaag tttctttgaa aatgatgttt ttacgcaaca ggctcgtttg tttgttgatc cactccaaac attccacccc tttttcaaaa tatacttcaa agattttggt tagaatatca aacccgtctt caaatttttt agggctagcc aaaacctttt tttggataga atcaaagatt tctaaagtcc cgtaataaat cgtgaaaaga atgttattag acaaaataaa ggtcgccatt tccgtatgga aagtctcatt ttcatcctgg ttagtgaaaa aggcgttgat cgtaacgctg gagctgtctt cccaatattt ggtaactgat gagactcgtt gggaatgatc tgtgttgtag tggatagcgt attcttggct tagagtggct aattcattgg gcgtggggtt gattaattca aaccataaaa cttcattttc ttctatatca aagccattaa aatcattaaa gcgtttttta atgacaaatt tttgctgttt gaaaaacacg ttcaccatat ggtggtggtg gtggtgng

Expected Protein Sequence

MAHHHHHHMV NVFFKQQKFV IKKRFNDFNG FDIEENEVLW FELINPTPNE LATLSQEYAI HYNTDHSQRV SSVTKYWEDS SSVTINAFFT NQDENETFHT EMATFILSNN ILFTIYYGTL EIFDSIQKKV LASPKKFEDG FDILTKIFEV YFEKGVECLE WINKQTSLLR KNIIFKETST HDDILVHLSN LQEFNVALRD SFFDKRRIIT ALLRSNKVDS DTKNNLNIIL TDFSSLVEST TVNLNSLDNI QNLFASQVNV EQNKIIK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga acgtgttttt caaacagcaa aaatttgtca ttaaaaaacg ctttaatgat tttaatggct ttgatataga agaaaatgaa gttttatggt ttgaattaat caaccccacg cccaatgaat tagccactct aagccaagaa tacgctatcc actacaacac agatcattcc caacgagtct catcagttac caaatattgg gaagacagct ccagcgttac gatcaacgcc tttttcacta accaggatga aaatgagact ttccatacgg aaatggcgac ctttattttg tctaataaca ttcttttcac gatttattac gggactttag aaatctttga ttctatccaa aaaaaggttt tggctagccc taaaaaattt gaagacgggt ttgatattct aaccaaaatc tttgaagtat attttgaaaa aggggtggaa tgtttggagt ggatcaacaa acaaacgagc ctgttgcgta aaaacatcat tttcaaagaa acttctacgc atgatgatat tttagtgcac ttgtccaatt tgcaagaatt taatgtggct ttaagggatt ccttttttga caaacggcgc attatcaccg ctttattaag gagcaataaa gtggatagcg ataccaaaaa taatttaaat atcattttaa ccgattttag ctctttggtg gagtctacaa cggtcaatct caactcgctt gataacattc aaaacctgtt cgcttctcaa gtcaatgtgg agcaaaataa aatcatcaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat