HepyC.19466.a
Biotin acetyl coenzyme A carboxylase synthetase
| CENTER ID: | HepyC.19466.a |
| ORGANISM: | Helicobacter pylori G27 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.19466.a.B1.GE41280 | full length | 1 | 212 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.19466.a.B1.PS38345 | full length | 1 | 212 |
Structures
6CK0
DEPOSITED: 2/27/2018
DETERMINATION: XRay
CLONE: HepyC.19466.a.B1.GE41280
PROTEIN: HepyC.19466.a.B1.PS38345
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|563041.6.peg.1179 |
| UniProt: | B5Z8D8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRQCEKRVFD SLPSTQTYLL EKLKNNELKA PILIVAKNQS TGIGSRGNIW EGTKSALTFS LALNASDLPK DLPMQANALY LGFLFKEVLK ELGSQTWLKW PNDLYLGDQK IGGVLVNVYK GMRVCGIGVN RVSKKWACLD IGASDDLIIE GFLKKIEENL FWGEVLSKYA LEFHRSNSFS FHNDWGELVS LKDAELLEDG RICIKGKIYD RM
NT Sequence
atgagacaat gtgaaaaaag agtttttgat agcctgcctt ccacgcaaac ctatctttta gaaaaactca aaaataacga actcaaagct cctattttga tcgtggctaa aaaccaaagc actgggatag gcagtagggg gaatatttgg gagggcacaa aaagcgcttt gactttttcg ctcgctttaa acgcaagcga tttgcctaaa gatttgccca tgcaagcgaa cgctttgtat ttagggtttt tattcaaaga agttttaaaa gaattaggct ctcaaacctg gcttaaatgg cctaacgatc tgtatttggg ggatcaaaaa atagggggcg tgttggttaa tgtttataaa ggcatgcggg tgtgcggcat tggcgtgaat agggtttcaa agaaatgggc atgtttagat attggcgcga gcgatgattt gattatagag ggctttttaa aaaaaataga agaaaatctt ttttgggggg aagttttaag taagtatgcg ttagaatttc atagaagcaa ctcttttagt ttccataatg attggggcga attggtgagt ttgaaagatg cggaattgtt agaagacggc cgcatttgta ttaaaggtaa gatttatgat aggatgtga
Details for HepyC.19466.a.B1.GE41280
| HARVESTED ON: | 3/10/2017 |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR QCEKRVFDSL PSTQTYLLEK LKNNELKAPI LIVAKNQSTG IGSRGNIWEG TKSALTFSLA LNASDLPKDL PMQANALYLG FLFKEVLKEL GSQTWLKWPN DLYLGDQKIG GVLVNVYKGM RVCGIGVNRV SKKWACLDIG ASDDLIIEGF LKKIEENLFW GEVLSKYALE FHRSNSFSFH NDWGELVSLK DAELLEDGRI CIKGKIYDRM
Validated NT Sequence
atggctcacc accaccacca ccatatgaga caatgtgaaa aaagagtttt tgatagcctg ccttccacgc aaacctatct tttagaaaaa ctcaaaaata acgaactcaa agctcctatt ttgatcgtgg ctaaaaacca aagcactggg ataggcagta gggggaatat ttgggagggc acaaaaagcg ctttgacttt ttcgctcgct ttaaacgcaa gcgatttgcc taaagatttg cccatgcaag cgaacgcttt gtatttaggg tttttattca aagaagtttt aaaagaatta ggctctcaaa cctggcttaa atggcctaac gatctgtatt tgggggatca aaaaataggg ggcgtgttgg ttaatgttta taaaggcatg cgggtgtgcg gcattggcgt gaatagggtt tcaaagaaat gggcatgttt agatattggc gcgagcgatg atttgattat agagggcttt ttaaaaaaaa tagaagaaaa tcttttttgg ggggaagttt taagtaagta tgcgttagaa tttcatagaa gcaactcttt tagtttccat aatgattggg gcgaattggt gagtttgaaa gatgcggaat tgttagaaga cggccgcatt tgtattaaag gtaagattta tgataggatg taagtgagta agat
Expected Protein Sequence
MAHHHHHHMR QCEKRVFDSL PSTQTYLLEK LKNNELKAPI LIVAKNQSTG IGSRGNIWEG TKSALTFSLA LNASDLPKDL PMQANALYLG FLFKEVLKEL GSQTWLKWPN DLYLGDQKIG GVLVNVYKGM RVCGIGVNRV SKKWACLDIG ASDDLIIEGF LKKIEENLFW GEVLSKYALE FHRSNSFSFH NDWGELVSLK DAELLEDGRI CIKGKIYDRM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagac aatgtgaaaa aagagttttt gatagcctgc cttccacgca aacctatctt ttagaaaaac tcaaaaataa cgaactcaaa gctcctattt tgatcgtggc taaaaaccaa agcactggga taggcagtag ggggaatatt tgggagggca caaaaagcgc tttgactttt tcgctcgctt taaacgcaag cgatttgcct aaagatttgc ccatgcaagc gaacgctttg tatttagggt ttttattcaa agaagtttta aaagaattag gctctcaaac ctggcttaaa tggcctaacg atctgtattt gggggatcaa aaaatagggg gcgtgttggt taatgtttat aaaggcatgc gggtgtgcgg cattggcgtg aatagggttt caaagaaatg ggcatgttta gatattggcg cgagcgatga tttgattata gagggctttt taaaaaaaat agaagaaaat cttttttggg gggaagtttt aagtaagtat gcgttagaat ttcatagaag caactctttt agtttccata atgattgggg cgaattggtg agtttgaaag atgcggaatt gttagaagac ggccgcattt gtattaaagg taagatttat gataggatgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for HepyC.19466.a.B1.PS38345
| PURIFICATION DATe: | 8/30/2017 |
| CONCENTRATION: | 35.04mg/ml |
| OBSERVED MW: | 22kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR QCEKRVFDSL PSTQTYLLEK LKNNELKAPI LIVAKNQSTG IGSRGNIWEG TKSALTFSLA LNASDLPKDL PMQANALYLG FLFKEVLKEL GSQTWLKWPN DLYLGDQKIG GVLVNVYKGM RVCGIGVNRV SKKWACLDIG ASDDLIIEGF LKKIEENLFW GEVLSKYALE FHRSNSFSFH NDWGELVSLK DAELLEDGRI CIKGKIYDRM
Validated NT Sequence
atggctcacc accaccacca ccatatgaga caatgtgaaa aaagagtttt tgatagcctg ccttccacgc aaacctatct tttagaaaaa ctcaaaaata acgaactcaa agctcctatt ttgatcgtgg ctaaaaacca aagcactggg ataggcagta gggggaatat ttgggagggc acaaaaagcg ctttgacttt ttcgctcgct ttaaacgcaa gcgatttgcc taaagatttg cccatgcaag cgaacgcttt gtatttaggg tttttattca aagaagtttt aaaagaatta ggctctcaaa cctggcttaa atggcctaac gatctgtatt tgggggatca aaaaataggg ggcgtgttgg ttaatgttta taaaggcatg cgggtgtgcg gcattggcgt gaatagggtt tcaaagaaat gggcatgttt agatattggc gcgagcgatg atttgattat agagggcttt ttaaaaaaaa tagaagaaaa tcttttttgg ggggaagttt taagtaagta tgcgttagaa tttcatagaa gcaactcttt tagtttccat aatgattggg gcgaattggt gagtttgaaa gatgcggaat tgttagaaga cggccgcatt tgtattaaag gtaagattta tgataggatg taagtgagta agat
Expressed Protein Sequence
MAHHHHHHMR QCEKRVFDSL PSTQTYLLEK LKNNELKAPI LIVAKNQSTG IGSRGNIWEG TKSALTFSLA LNASDLPKDL PMQANALYLG FLFKEVLKEL GSQTWLKWPN DLYLGDQKIG GVLVNVYKGM RVCGIGVNRV SKKWACLDIG ASDDLIIEGF LKKIEENLFW GEVLSKYALE FHRSNSFSFH NDWGELVSLK DAELLEDGRI CIKGKIYDRM
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAC AATGTGAAAA AAGAGTTTTT GATAGCCTGC CTTCCACGCA AACCTATCTT TTAGAAAAAC TCAAAAATAA CGAACTCAAA GCTCCTATTT TGATCGTGGC TAAAAACCAA AGCACTGGGA TAGGCAGTAG GGGGAATATT TGGGAGGGCA CAAAAAGCGC TTTGACTTTT TCGCTCGCTT TAAACGCAAG CGATTTGCCT AAAGATTTGC CCATGCAAGC GAACGCTTTG TATTTAGGGT TTTTATTCAA AGAAGTTTTA AAAGAATTAG GCTCTCAAAC CTGGCTTAAA TGGCCTAACG ATCTGTATTT GGGGGATCAA AAAATAGGGG GCGTGTTGGT TAATGTTTAT AAAGGCATGC GGGTGTGCGG CATTGGCGTG AATAGGGTTT CAAAGAAATG GGCATGTTTA GATATTGGCG CGAGCGATGA TTTGATTATA GAGGGCTTTT TAAAAAAAAT AGAAGAAAAT CTTTTTTGGG GGGAAGTTTT AAGTAAGTAT GCGTTAGAAT TTCATAGAAG CAACTCTTTT AGTTTCCATA ATGATTGGGG CGAATTGGTG AGTTTGAAAG ATGCGGAATT GTTAGAAGAC GGCCGCATTT GTATTAAAGG TAAGATTTAT GATAGGATGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT