HepyC.19463.a

Ubiquinol cytochrome c oxidoreductase, cytochrome c1

CENTER ID: HepyC.19463.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19463.a.B2.GE41278 soluble domain 25 285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1602
UniProt: B5Z9G9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEFKILIIL IVVVGVIYYG VEPYAHSVMH PKVAPADFAF KDLEPIDLKN GDANKGKQLV AENCTACHGI KSQNIPAPMD SLSASNSFGV VPPDLSHVAG VLNANFLARF IKDPVKTAKL SHKFNDERPY PMPAFSQFSD QDLSDIVAYL TSILPKSLSD KEVFAQSCQR CHSLDYAKDK AFSDPKDLAN YLGSHAPDLS MMIRAKGEHG LNVFINDPQK LLPGTAMPRV GLSEKAQKQV IAYLEKAGDR KKHERNTLGI KIMIFFAVLS FLAYAWKRKV WSEVH
NT Sequence
atgaaagaat ttaagattct aatcatcctt attgtggtgg taggcgtgat ttattatggg gttgagcctt atgcgcattc ggtgatgcac cctaaagtcg ctccggcaga ttttgctttc aaggatttag agccgattga tttaaaaaat ggcgacgcca ataagggcaa acaacttgta gccgaaaact gcaccgcttg ccatggcatt aaatcccaaa acattccagc ccctatggac agccttagcg cgagcaactc ttttggggtc gtgccaccgg atttaagcca tgtggcgggg gttttgaacg cgaatttctt agcccgcttc atcaaagacc ccgtgaaaac ggcgaaattg agccataaat tcaacgatga aaggccctat cctatgccgg cgttttctca atttagcgat caagatttga gcgatattgt ggcgtatctc acttctattt tgcctaaaag tttgagcgat aaggaagtgt ttgcgcaaag ctgtcaaagg tgccatagcc tggattatgc taaagataag gcctttagcg atcctaaaga tttagccaat tatttaggct ctcatgcacc tgatttgtcc atgatgatta gagctaaggg cgaacatggc ttgaatgttt tcatcaacga tccgcaaaag cttttgcctg gcacagctat gcctagagtg gggttgagtg aaaaagctca aaaacaagtc atcgcttatt tggaaaaagc gggcgatagg aaaaaacatg aaaggaatac tttagggatt aagatcatga ttttctttgc agtgctgtcg ttcttggctt atgcgtggaa aagaaaagtt tggagcgaag tgcattga
Details for HepyC.19463.a.B2.GE41278
HARVESTED ON: 3/10/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA HSVMHPKVAP ADFAFKDLEP IDLKNGDANK GKQLVAENCT ACHGIKSQNI PAPMDSLSAS NSFGVVPPDL SHVAGVLNAN FLARFIKDPV KTAKLSHKFN DERPYPMPAF SQFSDQDLSD IVAYLTSILP KSLSDKEVFA QSCQRCHSLD YAKDKAFSDP KDLANYLGSH APDLSMMIRA KGEHGLNVFI NDPQKLLPGT AMPRVGLSEK AQKQVIAYLE KAGDRKKHER NTLGIKIMIF FAVLSFLAYA WKXKVWSEVH
Validated NT Sequence
atggctcacc accaccacca ccatatggcg cattcggtga tgcaccctaa agtcgctccg gcagattttg ctttcaagga tttagagccg attgatttaa aaaatggcga cgccaataag ggcaaacaac ttgtagccga aaactgcacc gcttgccatg gcattaaatc ccaaaacatt ccagccccta tggacagcct tagcgcgagc aactcttttg gggtcgtgcc accggattta agccatgtgg cgggggtttt gaacgcgaat ttcttagccc gcttcatcaa agaccccgtg aaaacggcga aattgagcca taaattcaac gatgaaaggc cctatcctat gccggcgttt tctcaattta gcgatcaaga tttgagcgat attgtggcgt atctcacttc tattttgcct aaaagtttga gcgataagga agtgtttgcg caaagctgtc aaaggtgcca tagcctggat tatgctaaag ataaggcctt tagcgatcct aaagatttag ccaattattt aggctctcat gcacctgatt tgtccatgat gattagagct aagggcgaac atggcttgaa tgttttcatc aacgatccgc aaaagctttt gcctggcaca gctatgccta gagtggggtt gagtgaaaaa gctcaaaaac aagtcatcgc ttatttggaa aaagcgggcg ataggaaaaa acatgaaagg aatactttag ggattaagat catgattttc tttgcagtgc tgtcgttctt ggcttatgcg tggaaannna aagtttggag cgaagtgcat taagtgagta agat
Expected Protein Sequence
MAHHHHHHAH SVMHPKVAPA DFAFKDLEPI DLKNGDANKG KQLVAENCTA CHGIKSQNIP APMDSLSASN SFGVVPPDLS HVAGVLNANF LARFIKDPVK TAKLSHKFND ERPYPMPAFS QFSDQDLSDI VAYLTSILPK SLSDKEVFAQ SCQRCHSLDY AKDKAFSDPK DLANYLGSHA PDLSMMIRAK GEHGLNVFIN DPQKLLPGTA MPRVGLSEKA QKQVIAYLEK AGDRKKHERN TLGIKIMIFF AVLSFLAYAW KRKVWSEVH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcatt cggtgatgca ccctaaagtc gctccggcag attttgcttt caaggattta gagccgattg atttaaaaaa tggcgacgcc aataagggca aacaacttgt agccgaaaac tgcaccgctt gccatggcat taaatcccaa aacattccag cccctatgga cagccttagc gcgagcaact cttttggggt cgtgccaccg gatttaagcc atgtggcggg ggttttgaac gcgaatttct tagcccgctt catcaaagac cccgtgaaaa cggcgaaatt gagccataaa ttcaacgatg aaaggcccta tcctatgccg gcgttttctc aatttagcga tcaagatttg agcgatattg tggcgtatct cacttctatt ttgcctaaaa gtttgagcga taaggaagtg tttgcgcaaa gctgtcaaag gtgccatagc ctggattatg ctaaagataa ggcctttagc gatcctaaag atttagccaa ttatttaggc tctcatgcac ctgatttgtc catgatgatt agagctaagg gcgaacatgg cttgaatgtt ttcatcaacg atccgcaaaa gcttttgcct ggcacagcta tgcctagagt ggggttgagt gaaaaagctc aaaaacaagt catcgcttat ttggaaaaag cgggcgatag gaaaaaacat gaaaggaata ctttagggat taagatcatg attttctttg cagtgctgtc gttcttggct tatgcgtgga aaagaaaagt ttggagcgaa gtgcattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t