HepyC.19455.a

Zinc metalloprotease (EC 3.4.24.-)

CENTER ID: HepyC.19455.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.19455.a.B21.GE41728 soluble domain 115 278
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.265
UniProt: B5ZA24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIIAVLMLA FLIFVHELGH FTIARICGVK VEVFSIGFGK KLCFFKLFGT QFALSLIPLG GYVKLKGMDK EENEENKTHQ ANDSYAQKSP FQKLWILFGG AFFNFLFAIL VYFFLALSGE KVLLPVIGGL EKNALEAGLL KGDKILSINH KKIASFGEIR SVVARARGEL VLEIERNNQI LEKRLTPKIV AVISESNDPN EMIRYKAIGI KPDMQKMGVV SYSLIQAFKQ ALSRFKEGVV LIVDSLRRLI MGSASVKELS GVIGIVGALS HANSLSMLLL FGAFLSINLG ILNLLPIPAL DGAQMLGVVF KNIFHITLPT PIQNALWLAG VGFLVFVMFL GLFNDITRLL
NT Sequence
atgttcatta tagcggtttt aatgctggcg tttttaatct ttgtccatga attagggcat ttcacgatcg ctaggatttg tggggtgaaa gtggaagtgt ttagcattgg ttttggtaaa aaactctgtt tttttaagct ttttggcacg caattcgctc tgtctttgat cccgcttggg ggctatgtga aattaaaggg catggataaa gaagaaaatg aagaaaataa aactcatcaa gcaaatgata gctacgcgca aaaaagccct tttcaaaagc tatggatact atttgggggg gcgtttttca attttctttt tgcgatttta gtgtattttt ttctggcatt gagcggggaa aaagtcttac tgcccgtcat tggtggttta gaaaaaaacg cgctagaggc cgggctgtta aagggggata aaatcctttc tatcaaccac aaaaaaatag cgagttttgg agagattaga agcgtagtgg cgcgtgctag aggcgagttg gttttagaaa tagagcgcaa taatcagatt ttagaaaaac gactgacccc taaaatcgtg gcggtaataa gcgaatctaa tgatcctaat gaaatgatcc ggtataaagc gataggcatc aaaccggaca tgcaaaaaat gggcgttgtc tcttattcct taattcaggc gttcaagcag gccttgagtc ggtttaaaga gggcgttgtt ttgatcgtgg attctttaag gcgtttgatt atggggagcg cttcagttaa ggaattgagt ggggtaatag gcattgtggg agcgttaagc catgccaata gtttgagcat gcttttgttg tttggggcgt ttttgtccat caatttaggg attttaaact tattgcccat ccccgcctta gatggggcgc aaatgctagg ggttgttttt aaaaatattt ttcatatcac tttgccaaca cccatacaaa atgcgttgtg gctagcgggt gtggggtttt tggtttttgt catgttttta gggcttttca atgatattac tcgtttgcta taa
Details for HepyC.19455.a.B21.GE41728
HARVESTED ON: 6/19/2017
SEQUENCED ON: 6/22/2017
EXPECTED MW: 19kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML ALSGEKVLLP VIGGLEKNAL EAGLLKGDKI LSINHKKIAS FGEIRSVVAR ARGELVLEIE RNNQILEKRL TPKIVAVISE SNDPNEMIRY KAIGIKPDMQ KMGVVSYSLI QAFKQALSRF KEGVVLIVDS LRRLIMGSAS VKELSGVIGI VGALSHANSL SML
Validated NT Sequence
atcttactca cttaaagcat gctcaaacta ttggcatggc ttaacgctcc cacaatgcct attaccccac tcaattcctt aactgaagcg ctccccataa tcaaacgcct taaagaatcc acgatcaaaa caacgccctc tttaaaccga ctcaaggcct gcttgaacgc ctgaattaag gaataagaga caacgcccat tttttgcatg tccggtttga tgcctatcgc tttataccgg atcatttcat taggatcatt agattcgctt attaccgcca cgattttagg ggtcagtcgt ttttctaaaa tctgattatt gcgctctatt tctaaaacca actcgcctct agcacgcgcc actacgcttc taatctctcc aaaactcgct atttttttgt ggttgataga aaggatttta tcccccttta acagcccggc ctctagcgcg tttttttcta aaccaccaat gacgggcagt aagacttttt ccccgctcaa tgccagcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHLA LSGEKVLLPV IGGLEKNALE AGLLKGDKIL SINHKKIASF GEIRSVVARA RGELVLEIER NNQILEKRLT PKIVAVISES NDPNEMIRYK AIGIKPDMQK MGVVSYSLIQ AFKQALSRFK EGVVLIVDSL RRLIMGSASV KELSGVIGIV GALSHANSLS ML
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctggcat tgagcgggga aaaagtctta ctgcccgtca ttggtggttt agaaaaaaac gcgctagagg ccgggctgtt aaagggggat aaaatccttt ctatcaacca caaaaaaata gcgagttttg gagagattag aagcgtagtg gcgcgtgcta gaggcgagtt ggttttagaa atagagcgca ataatcagat tttagaaaaa cgactgaccc ctaaaatcgt ggcggtaata agcgaatcta atgatcctaa tgaaatgatc cggtataaag cgataggcat caaaccggac atgcaaaaaa tgggcgttgt ctcttattcc ttaattcagg cgttcaagca ggccttgagt cggtttaaag agggcgttgt tttgatcgtg gattctttaa ggcgtttgat tatggggagc gcttcagtta aggaattgag tggggtaata ggcattgtgg gagcgttaag ccatgccaat agtttgagca tgctttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat