SSGCID: Target Page

HepyC.18873.a

Diaminopimelate epimerase (DAP epimerase) (EC 5.1.1.7)

CENTER ID:	HepyC.18873.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.18873.a.B1.GE41271	full length		1	272

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.566
UniProt:	B5Z6T7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVFYKYSGSG NDFLITQSFK KKDFSNLAKQ VCHRHEGFGA DGLVVVLPSK DYDYEWDFYN SDGSKAGMCG NASRCVGLFA YQHAIAPKEH VFLAGKREIS IRIEEPNIIE SNLGNYKILD TIPALRCKKF FTNDSVLENI LTFYLIDTGV PHLVGFVKNK KLLNSLNTLE LRALRHEFNA NINIAFIENK ETIFLQTYER GVEDFTLACG TGMAAVFIAS HIFYNTPKKA ILIPKSNESL ELSLKNDGIF YKGAVRYIGM SVLGSVFENG CF

NT Sequence

atggtgtttt acaaatattc agggagcggg aatgattttt taatcacgca aagttttaaa aaaaaagatt tttccaattt agccaaacag gtgtgccata ggcatgaggg ttttggggct gatgggcttg tggtcgtctt accgagtaag gattatgact acgaatggga tttttacaat tcagacggct ctaaagctgg catgtgcggg aatgcgagcc gttgcgtggg gttatttgcc taccaacatg ctatagcccc taaagagcat gtttttttag ccgggaaaag agagatttct atccgtatag aagaacccaa tatcatagag agcaatctcg gtaactacaa aatcctagac accatacccg ccttaagatg caaaaaattt tttactaacg acagcgtttt agaaaatatc cttactttct atctcataga tacaggagtg ccgcatttag tgggatttgt gaaaaataaa aagttattaa attcccttaa cacgctagaa ttaagggctt taaggcatga atttaacgct aatattaaca tcgcttttat agaaaataaa gagacgattt ttttacaaac ttatgaaagg ggggttgaag atttcacgct agcttgcggg acaggcatgg cggcggtttt tatcgcctcg cacatttttt ataacacacc taaaaaagcc attctcatcc ctaaaagcaa cgaatcttta gagctttctt taaaaaatga tggaattttt tataagggtg cggtgcgtta tattggcatg agcgttttgg gcagtgtttt tgaaaatggg tgtttttga

Details for HepyC.18873.a.B1.GE41271

HARVESTED ON:	3/8/2017
SEQUENCED ON:	3/9/2017
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV FYKYSGSGND FLITQSFKKK DFSNLAKQVC HRHEGFGADG LVVVLPSKDY DYEWDFYNSD GSKAGMCGNA SRCVGLFAYQ HAIAPKEHVF LAGKREISIR IEEPNIIESN LGNYKILDTI PALRCKKFFT NDSVLENILT FYLIDTGVPH LVGFVKNKKL LNSLNTLELR ALRHEFNANI NIAFIENKET IFLQTYERGV EDFTLACGTG MAAVFIASHI FYNTPKKAIL IPKSNESLEL SLKNDGIFYK GAVRYIGMSV LGXXFENGCF

Validated NT Sequence

gatataccat ggctcaccac caccaccacc atatggtgtt ttacaaatat tcagggagcg ggaatgattt tttaatcacg caaagtttta aaaaaaaaga tttttccaat ttagccaaac aggtgtgcca taggcatgag ggttttgggg ctgatgggct tgtggtcgtc ttaccgagta aggattatga ctacgaatgg gatttttaca attcagacgg ctctaaagct ggcatgtgcg ggaatgcgag ccgttgcgtg gggttatttg cctaccaaca tgctatagcc cctaaagagc atgttttttt agccgggaaa agagagattt ctatccgtat agaagaaccc aatatcatag agagcaatct cggtaactac aaaatcctag acaccatacc cgccttaaga tgcaaaaaat tttttactaa cgacagcgtt ttagaaaata tccttacttt ctatctcata gatacaggag tgccgcattt agtgggattt gtgaaaaata aaaagttatt aaattccctt aacacgctag aattaagggc tttaaggcat gaatttaacg ctaatattaa catcgctttt atagaaaata aagagacgat ttttttacaa acttatgaaa ggggggttga agatttcacg ctagcttgcg ggacaggcat ggcggcggtt tttatcgcct cgcacatttt ttataacaca cctaaaaaag ccattctcat ccctaaaagc aacgaatctt tagagctttc tttaaaaaat gatggaattt tttataaggg tgcggtgcgt tatattggca tgagcgtttt gggcagnnnn tttgaaaatg ggtgttttta agtgagtaag at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgt tttacaaata ttcagggagc gggaatgatt ttttaatcac gcaaagtttt aaaaaaaaag atttttccaa tttagccaaa caggtgtgcc ataggcatga gggttttggg gctgatgggc ttgtggtcgt cttaccgagt aaggattatg actacgaatg ggatttttac aattcagacg gctctaaagc tggcatgtgc gggaatgcga gccgttgcgt ggggttattt gcctaccaac atgctatagc ccctaaagag catgtttttt tagccgggaa aagagagatt tctatccgta tagaagaacc caatatcata gagagcaatc tcggtaacta caaaatccta gacaccatac ccgccttaag atgcaaaaaa ttttttacta acgacagcgt tttagaaaat atccttactt tctatctcat agatacagga gtgccgcatt tagtgggatt tgtgaaaaat aaaaagttat taaattccct taacacgcta gaattaaggg ctttaaggca tgaatttaac gctaatatta acatcgcttt tatagaaaat aaagagacga tttttttaca aacttatgaa aggggggttg aagatttcac gctagcttgc gggacaggca tggcggcggt ttttatcgcc tcgcacattt tttataacac acctaaaaaa gccattctca tccctaaaag caacgaatct ttagagcttt ctttaaaaaa tgatggaatt ttttataagg gtgcggtgcg ttatattggc atgagcgttt tgggcagtgt ttttgaaaat gggtgttttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat