HepyC.18708.a

Isoleucine--tRNA ligase (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IleRS)

CENTER ID: HepyC.18708.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.18708.a.B1.GE41210 full length 1 920
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.18708.a.B1.PW38313 full length 1 920

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1460
UniProt: B5Z943

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQAFKR MQNRKDNHGD FTLHDGPPYA NGHLHLGHAL NKILKDIVIK REYFKGNKIY YTPGWDCHGL PIEQQILERL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD FEDPYKTMDF KFEASIYRAL VEVAKKGLLK ERHKPIYWSY ACESALAEAE VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW TLYANVAIAL KKDALYALTQ KGYLVAKVLH EKLAALGVVD SEIAHEFNAN DLEYLKATNP LNQRDSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLKY NLEVLMSVDE KGCYNEGIIH KKLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALN AIEKVEFVPS SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHVA NLFEKKGCDV WWESSVKDLL PPSYQEDSKH YEKVMHILDV WFDSGSTFKA VLEDYYGEKG QSPSDVILEG SDQHRGWFQS SLLIGCVLNN QAPFKKVITH GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ TFFIQTEQHY KKFRNTLKFL LANFSDMDLK DLERSHNFSP LDHFILETLE TTSTGVNSAF EEHDFVKGLN ILMAFVTNEL SGIYLDACKD SLYCDSKNNK KRQAIQMVLL AVASQLCYFL APILTHTIEE VLEHSQVLCA FLQAKDVFDL KGINILEKLR LKEFKKPENF EAVLALRSAF NEELDRLKKE GVIKNSLECA IEVKEKALRE NLVEELLMVS FVGVAKEKIS ETPAFTLFKA PFYKCPRCWR FKSELENTPC KRCEEVLKER
NT Sequence
gtgaaagaat acaaagacac cctaaactta aacacaacca ccttttctat gaaagggaat ttgagcgtta atgagcctaa aacttacgcc aaatggcaag agcaacaagc gttcaaacgc atgcaaaata gaaaagataa ccatggggat ttcactttgc atgacgggcc gccttatgcg aacgggcatt tgcatttagg gcatgcctta aacaaaattt taaaagacat tgttattaaa agagaatatt ttaaagggaa taaaatttat tacacgcccg gttgggattg ccatggcttg cccattgagc agcaaatttt agagcgatta gaaaaagaaa aaacgagcct ggaaaacccc acgctgttta gagaaaagtg ccgagatcat gcgaaaaaat ttttagaaat ccaaaagaat gaatttttgc aattaggcgt tttgggggat tttgaagatc cttataaaac catggatttt aaatttgaag cgagcattta tagggcttta gtggaagtgg ctaaaaaagg gcttttgaaa gaacgccata agcctattta ttggagttat gcatgcgaga gcgctttagc ggaagctgaa gtggaataca aaatgaaaaa atcgccctcc attttcgtgg cgtttggttt gaaaaaggag agtttagaaa agttaaaagt caaaaaagcg agcttggtga tttggacgac cacgccttgg actttgtatg cgaatgtggc gatcgctttg aaaaaagacg ccctttatgc gctcacccaa aaaggctatt tagtcgctaa agtcttgcat gaaaaattag ccgctttagg ggtggtggat agtgagattg cacatgaatt caatgccaat gatttagaat atttgaaggc caccaatcct ttaaaccaaa gagattccct aatcactcta ggagagcatg tcggtttaga agatggcaca ggagccgtgc ataccgcacc cgggcatggt gaagaggact actatttagg cttaaaatac aatttagaag tgttaatgtc tgtagatgaa aagggttgct ataatgaggg cattatccat aagaaattat tagatgaaag ctatctgggc gagcatgttt ttaaggctca aaaacgcatt atagagcaat tgggcgattc tttattgcta gagcaagaga ttgagcattc ctacccgcat tgttggagaa cgcacaagcc tgtgatttac agagcgacca cgcaatggtt tattttaatg gatgagcctt ttattcaaaa tgatggttct caaaaaacct taagagaagt ggctttgaat gcgattgaaa aggtggaatt tgtgccaagc agcgggaaaa accgcctaaa aaccatgata gaaaaccgcc ctgattggtg cttgagccgg caaagaaaat ggggcgtgcc actggccttt ttcatagaca aacgcacgaa taagccttgt tttgaaagcg aagttttaga gcatgtggcc aatctttttg aaaaaaaagg ctgtgatgtg tggtgggagt ctagcgtaaa agatttatta ccccctagct atcaagagga ctccaaacat tatgaaaaag tcatgcacat tttagacgtg tggtttgata gcggtagcac ctttaaggcg gttttagaag actattatgg agaaaagggg caaagcccta gcgatgtgat cttagaaggg agcgatcagc atagggggtg gtttcaaagc tcgcttttaa tcggttgtgt tttaaacaac caagcccctt ttaaaaaggt cattacgcat ggatttatcg tagatgaaaa gggcgagaaa atgagtaaat ctaagggcaa tgtggtgtct ttggacaact tactcaaaaa gcatgggagc gatgtggtgc gtttgtgggt agcgtttaat gactatcaaa acgatttgag ggtctctcaa accttcttca ttcaaacaga acagcattat aaaaaattcc gcaacacctt gaaattctta ctcgccaatt ttagcgatat ggatctcaag gatttagaac gctcccataa cttcagccct ttagatcatt ttatattaga gactttagaa acaacaagca ccggagtcaa tagcgcgttt gaagagcatg attttgtgaa aggcttgaat attttaatgg cgtttgttac caatgaattg agcgggattt atttagacgc ttgtaaggat agtttgtatt gcgatagcaa aaataataaa aaacgccaag ccattcaaat ggtcttactc gctgtggcca gtcaattgtg ctacttttta gccccgattt taacgcacac gattgaagaa gttttagagc atagccaggt gctttgcgcg tttttacaag ccaaagatgt gtttgatcta aaaggcatta atattttaga aaaactccgc cttaaagagt ttaaaaaacc agaaaatttt gaagccgttt tagccttgcg ttctgccttt aatgaagagt tagaccgatt gaaaaaagaa ggcgtcatta aaaattcgtt ggaatgcgct attgaagtga aagaaaaagc gttgcgtgaa aatttagtag aagaattgct gatggtgagc tttgtagggg ttgcaaaaga aaaaataagc gaaacgccag cattcacgct ctttaaagcc cccttttata aatgccctag gtgttggcgt ttcaaaagcg agctagaaaa caccccttgc aagcgctgcg aagaggtttt aaaagagcga tga
Details for HepyC.18708.a.B1.GE41210
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 107kDa
OBSERVED MW: 105kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK EYKDTLNLNT TTFSMKGNLS VNEPKTYAKW QEQQAFKRMQ NRKDNHGDFT LHDGPPYANG HLHLGHALNK ILKDIVIKRE YFKGNKIYYT PGWDCHGLPI EQQILERLEK EKTSLENPTL FREKCRDHAK KFLEIQKNEF LQLGVLGDFE DPYKTMDFKF EASIYRALVE VAKKGLLKER HKPIYWSYAC ESALAEAEVE YKMKKSPSIF VAFGLKKESL EKLKVKKASL VIWTTTPWTL YANVAIALKK DALYALTQKG YLVAKVLHEK LAALGVVDSE IAHEFNANDL EYLKATNPLN QRDSLITLGE HVGLEDGTGA VHTAPGHGEE DYYLGLKYNL EVLMSVDEKG CYNEGIIHKK LLDESYLGEH VFKAQKRIIE QLGDSLLLEQ EIEHSYPHCW RTHKPVIYRA TTQWFILMDE PFIQNDGSQK TLREVALNAI EKVEFVPSSG KNRLKTMIEN RPDWCLSRQR KWGVPLAFFI DKRTNKPCFE SEVLEHVANL FEKKGCDVWW ESSVKDLLPP SYQEDSKHYE KVMHILDVWF DSGSTFKAVL EDYYGEKGQS PSDVILEGSD QHRGWFQSSL LIGCVLNNQA PFKKVITHGF IVDEKGEKMS KSKGNVVSLD NLLKKHGSDV VRLWVAFNDY QNDLRVSQTF FIQTEQHYKK FRNTLKFLLA NFSDMDLKDL ERSHNFSPLD HFILETLETT STGVNSAFEE HDFVKGLNIL MAFVTNELSG IYLDACKDSL YCDSKNNKKR QAIQMVLLAV ASQLCYFLAP ILTHTIEEVL EHSQVLCAFL QAKDVFDLKG INILEKLRLK EFKKPENFEA VLALRSAFNE ELDRLKKEGV IKNSLECAIE VKEKALRENL VEELLMVSFV GVAKEKISET PAFTLFKAPF YKCPRCWRFK SELENTPCKR CEEVLKER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaag aatacaaaga caccctaaac ttaaacacaa ccaccttttc tatgaaaggg aatttgagcg ttaatgagcc taaaacttac gccaaatggc aagagcaaca agcgttcaaa cgcatgcaaa atagaaaaga taaccatggg gatttcactt tgcatgacgg gccgccttat gcgaacgggc atttgcattt agggcatgcc ttaaacaaaa ttttaaaaga cattgttatt aaaagagaat attttaaagg gaataaaatt tattacacgc ccggttggga ttgccatggc ttgcccattg agcagcaaat tttagagcga ttagaaaaag aaaaaacgag cctggaaaac cccacgctgt ttagagaaaa gtgccgagat catgcgaaaa aatttttaga aatccaaaag aatgaatttt tgcaattagg cgttttgggg gattttgaag atccttataa aaccatggat tttaaatttg aagcgagcat ttatagggct ttagtggaag tggctaaaaa agggcttttg aaagaacgcc ataagcctat ttattggagt tatgcatgcg agagcgcttt agcggaagct gaagtggaat acaaaatgaa aaaatcgccc tccattttcg tggcgtttgg tttgaaaaag gagagtttag aaaagttaaa agtcaaaaaa gcgagcttgg tgatttggac gaccacgcct tggactttgt atgcgaatgt ggcgatcgct ttgaaaaaag acgcccttta tgcgctcacc caaaaaggct atttagtcgc taaagtcttg catgaaaaat tagccgcttt aggggtggtg gatagtgaga ttgcacatga attcaatgcc aatgatttag aatatttgaa ggccaccaat cctttaaacc aaagagattc cctaatcact ctaggagagc atgtcggttt agaagatggc acaggagccg tgcataccgc acccgggcat ggtgaagagg actactattt aggcttaaaa tacaatttag aagtgttaat gtctgtagat gaaaagggtt gctataatga gggcattatc cataagaaat tattagatga aagctatctg ggcgagcatg tttttaaggc tcaaaaacgc attatagagc aattgggcga ttctttattg ctagagcaag agattgagca ttcctacccg cattgttgga gaacgcacaa gcctgtgatt tacagagcga ccacgcaatg gtttatttta atggatgagc cttttattca aaatgatggt tctcaaaaaa ccttaagaga agtggctttg aatgcgattg aaaaggtgga atttgtgcca agcagcggga aaaaccgcct aaaaaccatg atagaaaacc gccctgattg gtgcttgagc cggcaaagaa aatggggcgt gccactggcc tttttcatag acaaacgcac gaataagcct tgttttgaaa gcgaagtttt agagcatgtg gccaatcttt ttgaaaaaaa aggctgtgat gtgtggtggg agtctagcgt aaaagattta ttacccccta gctatcaaga ggactccaaa cattatgaaa aagtcatgca cattttagac gtgtggtttg atagcggtag cacctttaag gcggttttag aagactatta tggagaaaag gggcaaagcc ctagcgatgt gatcttagaa gggagcgatc agcatagggg gtggtttcaa agctcgcttt taatcggttg tgttttaaac aaccaagccc cttttaaaaa ggtcattacg catggattta tcgtagatga aaagggcgag aaaatgagta aatctaaggg caatgtggtg tctttggaca acttactcaa aaagcatggg agcgatgtgg tgcgtttgtg ggtagcgttt aatgactatc aaaacgattt gagggtctct caaaccttct tcattcaaac agaacagcat tataaaaaat tccgcaacac cttgaaattc ttactcgcca attttagcga tatggatctc aaggatttag aacgctccca taacttcagc cctttagatc attttatatt agagacttta gaaacaacaa gcaccggagt caatagcgcg tttgaagagc atgattttgt gaaaggcttg aatattttaa tggcgtttgt taccaatgaa ttgagcggga tttatttaga cgcttgtaag gatagtttgt attgcgatag caaaaataat aaaaaacgcc aagccattca aatggtctta ctcgctgtgg ccagtcaatt gtgctacttt ttagccccga ttttaacgca cacgattgaa gaagttttag agcatagcca ggtgctttgc gcgtttttac aagccaaaga tgtgtttgat ctaaaaggca ttaatatttt agaaaaactc cgccttaaag agtttaaaaa accagaaaat tttgaagccg ttttagcctt gcgttctgcc tttaatgaag agttagaccg attgaaaaaa gaaggcgtca ttaaaaattc gttggaatgc gctattgaag tgaaagaaaa agcgttgcgt gaaaatttag tagaagaatt gctgatggtg agctttgtag gggttgcaaa agaaaaaata agcgaaacgc cagcattcac gctctttaaa gccccctttt ataaatgccc taggtgttgg cgtttcaaaa gcgagctaga aaacacccct tgcaagcgct gcgaagaggt tttaaaagag cgatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for HepyC.18708.a.B1.PW38313
PURIFICATION DATe: 7/17/2017
CONCENTRATION: 11.32mg/ml
OBSERVED MW: 110kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK EYKDTLNLNT TTFSMKGNLS VNEPKTYAKW QEQQAFKRMQ NRKDNHGDFT LHDGPPYANG HLHLGHALNK ILKDIVIKRE YFKGNKIYYT PGWDCHGLPI EQQILERLEK EKTSLENPTL FREKCRDHAK KFLEIQKNEF LQLGVLGDFE DPYKTMDFKF EASIYRALVE VAKKGLLKER HKPIYWSYAC ESALAEAEVE YKMKKSPSIF VAFGLKKESL EKLKVKKASL VIWTTTPWTL YANVAIALKK DALYALTQKG YLVAKVLHEK LAALGVVDSE IAHEFNANDL EYLKATNPLN QRDSLITLGE HVGLEDGTGA VHTAPGHGEE DYYLGLKYNL EVLMSVDEKG CYNEGIIHKK LLDESYLGEH VFKAQKRIIE QLGDSLLLEQ EIEHSYPHCW RTHKPVIYRA TTQWFILMDE PFIQNDGSQK TLREVALNAI EKVEFVPSSG KNRLKTMIEN RPDWCLSRQR KWGVPLAFFI DKRTNKPCFE SEVLEHVANL FEKKGCDVWW ESSVKDLLPP SYQEDSKHYE KVMHILDVWF DSGSTFKAVL EDYYGEKGQS PSDVILEGSD QHRGWFQSSL LIGCVLNNQA PFKKVITHGF IVDEKGEKMS KSKGNVVSLD NLLKKHGSDV VRLWVAFNDY QNDLRVSQTF FIQTEQHYKK FRNTLKFLLA NFSDMDLKDL ERSHNFSPLD HFILETLETT STGVNSAFEE HDFVKGLNIL MAFVTNELSG IYLDACKDSL YCDSKNNKKR QAIQMVLLAV ASQLCYFLAP ILTHTIEEVL EHSQVLCAFL QAKDVFDLKG INILEKLRLK EFKKPENFEA VLALRSAFNE ELDRLKKEGV IKNSLECAIE VKEKALRENL VEELLMVSFV GVAKEKISET PAFTLFKAPF YKCPRCWRFK SELENTPCKR CEEVLKER
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAAAG AATACAAAGA CACCCTAAAC TTAAACACAA CCACCTTTTC TATGAAAGGG AATTTGAGCG TTAATGAGCC TAAAACTTAC GCCAAATGGC AAGAGCAACA AGCGTTCAAA CGCATGCAAA ATAGAAAAGA TAACCATGGG GATTTCACTT TGCATGACGG GCCGCCTTAT GCGAACGGGC ATTTGCATTT AGGGCATGCC TTAAACAAAA TTTTAAAAGA CATTGTTATT AAAAGAGAAT ATTTTAAAGG GAATAAAATT TATTACACGC CCGGTTGGGA TTGCCATGGC TTGCCCATTG AGCAGCAAAT TTTAGAGCGA TTAGAAAAAG AAAAAACGAG CCTGGAAAAC CCCACGCTGT TTAGAGAAAA GTGCCGAGAT CATGCGAAAA AATTTTTAGA AATCCAAAAG AATGAATTTT TGCAATTAGG CGTTTTGGGG GATTTTGAAG ATCCTTATAA AACCATGGAT TTTAAATTTG AAGCGAGCAT TTATAGGGCT TTAGTGGAAG TGGCTAAAAA AGGGCTTTTG AAAGAACGCC ATAAGCCTAT TTATTGGAGT TATGCATGCG AGAGCGCTTT AGCGGAAGCT GAAGTGGAAT ACAAAATGAA AAAATCGCCC TCCATTTTCG TGGCGTTTGG TTTGAAAAAG GAGAGTTTAG AAAAGTTAAA AGTCAAAAAA GCGAGCTTGG TGATTTGGAC GACCACGCCT TGGACTTTGT ATGCGAATGT GGCGATCGCT TTGAAAAAAG ACGCCCTTTA TGCGCTCACC CAAAAAGGCT ATTTAGTCGC TAAAGTCTTG CATGAAAAAT TAGCCGCTTT AGGGGTGGTG GATAGTGAGA TTGCACATGA ATTCAATGCC AATGATTTAG AATATTTGAA GGCCACCAAT CCTTTAAACC AAAGAGATTC CCTAATCACT CTAGGAGAGC ATGTCGGTTT AGAAGATGGC ACAGGAGCCG TGCATACCGC ACCCGGGCAT GGTGAAGAGG ACTACTATTT AGGCTTAAAA TACAATTTAG AAGTGTTAAT GTCTGTAGAT GAAAAGGGTT GCTATAATGA GGGCATTATC CATAAGAAAT TATTAGATGA AAGCTATCTG GGCGAGCATG TTTTTAAGGC TCAAAAACGC ATTATAGAGC AATTGGGCGA TTCTTTATTG CTAGAGCAAG AGATTGAGCA TTCCTACCCG CATTGTTGGA GAACGCACAA GCCTGTGATT TACAGAGCGA CCACGCAATG GTTTATTTTA ATGGATGAGC CTTTTATTCA AAATGATGGT TCTCAAAAAA CCTTAAGAGA AGTGGCTTTG AATGCGATTG AAAAGGTGGA ATTTGTGCCA AGCAGCGGGA AAAACCGCCT AAAAACCATG ATAGAAAACC GCCCTGATTG GTGCTTGAGC CGGCAAAGAA AATGGGGCGT GCCACTGGCC TTTTTCATAG ACAAACGCAC GAATAAGCCT TGTTTTGAAA GCGAAGTTTT AGAGCATGTG GCCAATCTTT TTGAAAAAAA AGGCTGTGAT GTGTGGTGGG AGTCTAGCGT AAAAGATTTA TTACCCCCTA GCTATCAAGA GGACTCCAAA CATTATGAAA AAGTCATGCA CATTTTAGAC GTGTGGTTTG ATAGCGGTAG CACCTTTAAG GCGGTTTTAG AAGACTATTA TGGAGAAAAG GGGCAAAGCC CTAGCGATGT GATCTTAGAA GGGAGCGATC AGCATAGGGG GTGGTTTCAA AGCTCGCTTT TAATCGGTTG TGTTTTAAAC AACCAAGCCC CTTTTAAAAA GGTCATTACG CATGGATTTA TCGTAGATGA AAAGGGCGAG AAAATGAGTA AATCTAAGGG CAATGTGGTG TCTTTGGACA ACTTACTCAA AAAGCATGGG AGCGATGTGG TGCGTTTGTG GGTAGCGTTT AATGACTATC AAAACGATTT GAGGGTCTCT CAAACCTTCT TCATTCAAAC AGAACAGCAT TATAAAAAAT TCCGCAACAC CTTGAAATTC TTACTCGCCA ATTTTAGCGA TATGGATCTC AAGGATTTAG AACGCTCCCA TAACTTCAGC CCTTTAGATC ATTTTATATT AGAGACTTTA GAAACAACAA GCACCGGAGT CAATAGCGCG TTTGAAGAGC ATGATTTTGT GAAAGGCTTG AATATTTTAA TGGCGTTTGT TACCAATGAA TTGAGCGGGA TTTATTTAGA CGCTTGTAAG GATAGTTTGT ATTGCGATAG CAAAAATAAT AAAAAACGCC AAGCCATTCA AATGGTCTTA CTCGCTGTGG CCAGTCAATT GTGCTACTTT TTAGCCCCGA TTTTAACGCA CACGATTGAA GAAGTTTTAG AGCATAGCCA GGTGCTTTGC GCGTTTTTAC AAGCCAAAGA TGTGTTTGAT CTAAAAGGCA TTAATATTTT AGAAAAACTC CGCCTTAAAG AGTTTAAAAA ACCAGAAAAT TTTGAAGCCG TTTTAGCCTT GCGTTCTGCC TTTAATGAAG AGTTAGACCG ATTGAAAAAA GAAGGCGTCA TTAAAAATTC GTTGGAATGC GCTATTGAAG TGAAAGAAAA AGCGTTGCGT GAAAATTTAG TAGAAGAATT GCTGATGGTG AGCTTTGTAG GGGTTGCAAA AGAAAAAATA AGCGAAACGC CAGCATTCAC GCTCTTTAAA GCCCCCTTTT ATAAATGCCC TAGGTGTTGG CGTTTCAAAA GCGAGCTAGA AAACACCCCT TGCAAGCGCT GCGAAGAGGT TTTAAAAGAG CGATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT