HepyC.18178.a

Lon protease (EC 3.4.21.53) (ATP-dependent protease La)

CENTER ID: HepyC.18178.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.18178.a.B1.GE41204 full length 1 825
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.18178.a.B1.PS38298 full length 1 825

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1434
UniProt: B5Z923

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEDFPKILP LLVEEDTFLY PFMIAPIFLQ NNASIKAVTY AKNNKSLVFI ACQKDKLNDN EAPYYDVGVI GSIMREANMP NGRVKLLFNG IAKGRILEPA KENEQGFLEA QISPIEYLEY DKENIQAIVE VLKEKVITLA NVSSLFPPDL IKALEDNDDP NRIADLIAAA LHLKRDQAYS LFANNNTEQR LLDLIDIVIE ETKTQKLQKE IKSKVHQKME QTNKEYFLKE QLKQIQKELG TDKQRDEDLN QYYQKLESIK PFLKEEAFKE IKKQIDRLSR THADSSDSAT LQNYIETMLD VPFGQYGKKA LDIKHVREQL DKDHYSLKRP KERIVEYFAT MQLLEMRRKK KPEKKDKTKG TILCFYGPPG VGKTSLANSI AKAIERPLVR IALGGLEDVN ELRGHRRTYI GSMPGRIVQG LIEAKKMNPV MVLDEIDKVD RSVRGDPASA LLEILDPEQN IAFRDHYANF SIDLSQVIFI ATANNIDRIP APLRDRMEFI SVSSYTPSEK EEIAKNYLIP QELEKHALKP SEVDISHECL KLIIEKYTRE AGVRDLRRQI ATIMRKAALK YLEDNPHKKG RTKKGEDKKS ENQRDENKDF CVSITPDNLK EYLERMVFEI DPIDEENKIG IINGLAWTPV GGDVLKIEAV KIRGKGELKL TGSLGDVMKE SAIIAFSVVK VLLDNETLKA PKIPSETDAE GKKKKKALKV YNAYDLHLHV PEGATPKDGP SAGIAMASVM ASILCDRATR SEVAMTGELT LSGEVLPIGG LKEKLIAAFK AGIKTALIPV KNYERDLDEI PAEVRENLNI VAVKNIAEVL EKTLL
NT Sequence
atgactgaag atttccctaa aattctgcct ttattggtag aagaagacac ttttttatac ccctttatga tagcccctat ttttttgcaa aataacgcga gcattaaggc ggtaacttac gctaaaaaca ataaatcgtt agtctttatt gcatgccaaa aagacaaatt gaacgataat gaagcccctt attatgatgt gggggtgatt ggatctatta tgcgtgaagc caacatgcct aatgggcgcg tgaaattgct ctttaatggc atcgctaagg ggcgtatttt agagcctgct aaagaaaacg agcaaggctt tttagaagct caaataagcc ctattgaata tttagaatac gataaagaaa acattcaagc gatcgtggaa gtcttaaaag aaaaagtgat cactctagcc aatgtcagct cactctttcc tccggatttg atcaaggctt tagaagacaa tgacgatcct aaccgcatcg ctgatttaat cgcagcggcc ttgcatttga aaagagatca agcgtattct ctttttgcga acaacaacac cgaacagcgc ttgttggatt tgattgatat tgtgatagaa gagactaaaa cccaaaaact ccaaaaagaa atcaaatcca aagtccatca aaaaatggag caaaccaata aggaatattt cttaaaagag cagctcaaac aaatccaaaa agagcttggc acagacaaac agcgagatga agatttaaac caatactacc aaaaactaga aagcatcaag cctttcttaa aagaagaagc gtttaaggag attaaaaagc aaattgaccg gctgagccga acccatgcgg atagttcaga tagcgcgact ttacaaaatt atattgaaac catgttagat gtgccttttg ggcaatacgg gaaaaaagcg cttgacatta agcatgtgag agaacaacta gacaaagatc attattcctt aaaaaggcct aaagagcgta ttgtagaata ctttgccacc atgcagcttt tagaaatgcg ccgcaagaaa aagccagaaa aaaaagacaa aactaaaggc acgattttat gcttttatgg gcctcctggc gtgggtaaaa cgagcttggc taattccatc gctaaagcga tagagcgccc tttagttcgg atcgctttag ggggcttaga agatgtgaat gaattaagag ggcacagacg cacttatata ggctccatgc ccgggcgcat tgtccaaggg ctcattgaag ccaaaaagat gaatccggtc atggttttag atgaaattga taaggtggat agaagcgtta gaggcgatcc agcgagcgct ttattagaga tcttagaccc tgagcaaaat atcgctttta gggatcatta tgcgaatttc agcattgatt tatcgcaagt gatttttatc gctaccgcta ataatattga caggatccca gcccctttaa gagacagaat ggaatttatc agcgtgtcca gctacacgcc tagcgaaaaa gaagagatcg ctaaaaacta cctcatcccc caagaattag aaaagcacgc cttaaagcct agcgaagtgg atattagcca tgagtgtttg aaactcatta ttgaaaaata caccagagaa gcgggcgtta gggatttacg aagacagatc gcaacgatta tgcgtaaagc ggctttaaaa tacctagaag ataacccgca caaaaaaggg cgaactaaaa aaggcgagga taaaaaaagc gaaaatcaaa gagacgagaa caaagatttc tgcgtctcta tcacgcctga taaccttaaa gagtatttag agcgcatggt gtttgaaatt gaccccatag atgaagaaaa taaaatcggt attatcaatg gtttggcatg gactccagtg ggcggtgatg tgcttaaaat tgaagcggtt aagatcaggg gtaaggggga attgaaactc accgggagtt taggcgatgt gatgaaagaa tccgccatta ttgccttttc tgttgtcaaa gtcttattgg ataacgaaac cttaaaagcg cctaaaatcc ctagcgagac cgatgcagag ggcaagaaaa agaaaaaagc gctgaaagtt tataacgctt atgatttgca cttgcatgtc cctgaggggg ctacgcctaa agacggcccg agcgctggga tcgctatggc gagcgtgatg gcgagcattt tgtgcgacag ggctacaaga agcgaagtgg caatgacggg cgaattgact ttgagcgggg aagttttacc cataggaggg ttgaaagaaa aattgattgc cgcttttaaa gccggcatta aaaccgctct cattcctgtc aaaaattacg aaagggattt agacgagatc cctgctgaag tgcgagagaa tttaaacatc gttgcggtga aaaacatcgc tgaagtgtta gaaaaaacct tactttag
Details for HepyC.18178.a.B1.GE41204
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 94kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT EDFPKILPLL VEEDTFLYPF MIAPIFLQNN ASIKAVTYAK NNKSLVFIAC QKDKLNDNEA PYYDVGVIGS IMREANMPNG RVKLLFNGIA KGRILEPAKE NEQGFLEAQI SPIEYLEYDK ENIQAIVEVL KEKVITLANV SSLFPPDLIK ALEDNDDPNR IADLIAAALH LKRDQAYSLF ANNNTEQRLL DLIDIVIEET KTQKLQKEIK SKVHQKMEQT NKEYFLKEQL KQIQKELGTD KQRDEDLNQY YQKLESIKPF LKEEAFKEIK KQIDRLSRTH ADSSDSATLQ NYIETMLDVP FGQYGKKALD IKHVREQLDK DHYSLKRPKE RIVEYFATMQ LLEMRRKKKP EKKDKTKGTI LCFYGPPGVG KTSLANSIAK AIERPLVRIA LGGLEDVNEL RGHRRTYIGS MPGRIVQGLI EAKKMNPVMV LDEIDKVDRS VRGDPASALL EILDPEQNIA FRDHYANFSI DLSQVIFIAT ANNIDRIPAP LRDRMEFISV SSYTPSEKEE IAKNYLIPQE LEKHALKPSE VDISHECLKL IIEKYTREAG VRDLRRQIAT IMRKAALKYL EDNPHKKGRT KKGEDKKSEN QRDENKDFCV SITPDNLKEY LERMVFEIDP IDEENKIGII NGLAWTPVGG DVLKIEAVKI RGKGELKLTG SLGDVMKESA IIAFSVVKVL LDNETLKAPK IPSETDAEGK KKKKALKVYN AYDLHLHVPE GATPKDGPSA GIAMASVMAS ILCDRATRSE VAMTGELTLS GEVLPIGGLK EKLIAAFKAG IKTALIPVKN YERDLDEIPA EVRENLNIVA VKNIAEVLEK TLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aagatttccc taaaattctg cctttattgg tagaagaaga cactttttta taccccttta tgatagcccc tatttttttg caaaataacg cgagcattaa ggcggtaact tacgctaaaa acaataaatc gttagtcttt attgcatgcc aaaaagacaa attgaacgat aatgaagccc cttattatga tgtgggggtg attggatcta ttatgcgtga agccaacatg cctaatgggc gcgtgaaatt gctctttaat ggcatcgcta aggggcgtat tttagagcct gctaaagaaa acgagcaagg ctttttagaa gctcaaataa gccctattga atatttagaa tacgataaag aaaacattca agcgatcgtg gaagtcttaa aagaaaaagt gatcactcta gccaatgtca gctcactctt tcctccggat ttgatcaagg ctttagaaga caatgacgat cctaaccgca tcgctgattt aatcgcagcg gccttgcatt tgaaaagaga tcaagcgtat tctctttttg cgaacaacaa caccgaacag cgcttgttgg atttgattga tattgtgata gaagagacta aaacccaaaa actccaaaaa gaaatcaaat ccaaagtcca tcaaaaaatg gagcaaacca ataaggaata tttcttaaaa gagcagctca aacaaatcca aaaagagctt ggcacagaca aacagcgaga tgaagattta aaccaatact accaaaaact agaaagcatc aagcctttct taaaagaaga agcgtttaag gagattaaaa agcaaattga ccggctgagc cgaacccatg cggatagttc agatagcgcg actttacaaa attatattga aaccatgtta gatgtgcctt ttgggcaata cgggaaaaaa gcgcttgaca ttaagcatgt gagagaacaa ctagacaaag atcattattc cttaaaaagg cctaaagagc gtattgtaga atactttgcc accatgcagc ttttagaaat gcgccgcaag aaaaagccag aaaaaaaaga caaaactaaa ggcacgattt tatgctttta tgggcctcct ggcgtgggta aaacgagctt ggctaattcc atcgctaaag cgatagagcg ccctttagtt cggatcgctt tagggggctt agaagatgtg aatgaattaa gagggcacag acgcacttat ataggctcca tgcccgggcg cattgtccaa gggctcattg aagccaaaaa gatgaatccg gtcatggttt tagatgaaat tgataaggtg gatagaagcg ttagaggcga tccagcgagc gctttattag agatcttaga ccctgagcaa aatatcgctt ttagggatca ttatgcgaat ttcagcattg atttatcgca agtgattttt atcgctaccg ctaataatat tgacaggatc ccagcccctt taagagacag aatggaattt atcagcgtgt ccagctacac gcctagcgaa aaagaagaga tcgctaaaaa ctacctcatc ccccaagaat tagaaaagca cgccttaaag cctagcgaag tggatattag ccatgagtgt ttgaaactca ttattgaaaa atacaccaga gaagcgggcg ttagggattt acgaagacag atcgcaacga ttatgcgtaa agcggcttta aaatacctag aagataaccc gcacaaaaaa gggcgaacta aaaaaggcga ggataaaaaa agcgaaaatc aaagagacga gaacaaagat ttctgcgtct ctatcacgcc tgataacctt aaagagtatt tagagcgcat ggtgtttgaa attgacccca tagatgaaga aaataaaatc ggtattatca atggtttggc atggactcca gtgggcggtg atgtgcttaa aattgaagcg gttaagatca ggggtaaggg ggaattgaaa ctcaccggga gtttaggcga tgtgatgaaa gaatccgcca ttattgcctt ttctgttgtc aaagtcttat tggataacga aaccttaaaa gcgcctaaaa tccctagcga gaccgatgca gagggcaaga aaaagaaaaa agcgctgaaa gtttataacg cttatgattt gcacttgcat gtccctgagg gggctacgcc taaagacggc ccgagcgctg ggatcgctat ggcgagcgtg atggcgagca ttttgtgcga cagggctaca agaagcgaag tggcaatgac gggcgaattg actttgagcg gggaagtttt acccatagga gggttgaaag aaaaattgat tgccgctttt aaagccggca ttaaaaccgc tctcattcct gtcaaaaatt acgaaaggga tttagacgag atccctgctg aagtgcgaga gaatttaaac atcgttgcgg tgaaaaacat cgctgaagtg ttagaaaaaa ccttactttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for HepyC.18178.a.B1.PS38298
PURIFICATION DATe: 6/6/2017
CONCENTRATION: 22.6mg/ml
OBSERVED MW: 80kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer: 20mM HEPES, 300mM NaCl, 5% Glycerol, 1mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMT EDFPKILPLL VEEDTFLYPF MIAPIFLQNN ASIKAVTYAK NNKSLVFIAC QKDKLNDNEA PYYDVGVIGS IMREANMPNG RVKLLFNGIA KGRILEPAKE NEQGFLEAQI SPIEYLEYDK ENIQAIVEVL KEKVITLANV SSLFPPDLIK ALEDNDDPNR IADLIAAALH LKRDQAYSLF ANNNTEQRLL DLIDIVIEET KTQKLQKEIK SKVHQKMEQT NKEYFLKEQL KQIQKELGTD KQRDEDLNQY YQKLESIKPF LKEEAFKEIK KQIDRLSRTH ADSSDSATLQ NYIETMLDVP FGQYGKKALD IKHVREQLDK DHYSLKRPKE RIVEYFATMQ LLEMRRKKKP EKKDKTKGTI LCFYGPPGVG KTSLANSIAK AIERPLVRIA LGGLEDVNEL RGHRRTYIGS MPGRIVQGLI EAKKMNPVMV LDEIDKVDRS VRGDPASALL EILDPEQNIA FRDHYANFSI DLSQVIFIAT ANNIDRIPAP LRDRMEFISV SSYTPSEKEE IAKNYLIPQE LEKHALKPSE VDISHECLKL IIEKYTREAG VRDLRRQIAT IMRKAALKYL EDNPHKKGRT KKGEDKKSEN QRDENKDFCV SITPDNLKEY LERMVFEIDP IDEENKIGII NGLAWTPVGG DVLKIEAVKI RGKGELKLTG SLGDVMKESA IIAFSVVKVL LDNETLKAPK IPSETDAEGK KKKKALKVYN AYDLHLHVPE GATPKDGPSA GIAMASVMAS ILCDRATRSE VAMTGELTLS GEVLPIGGLK EKLIAAFKAG IKTALIPVKN YERDLDEIPA EVRENLNIVA VKNIAEVLEK TLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG AAGATTTCCC TAAAATTCTG CCTTTATTGG TAGAAGAAGA CACTTTTTTA TACCCCTTTA TGATAGCCCC TATTTTTTTG CAAAATAACG CGAGCATTAA GGCGGTAACT TACGCTAAAA ACAATAAATC GTTAGTCTTT ATTGCATGCC AAAAAGACAA ATTGAACGAT AATGAAGCCC CTTATTATGA TGTGGGGGTG ATTGGATCTA TTATGCGTGA AGCCAACATG CCTAATGGGC GCGTGAAATT GCTCTTTAAT GGCATCGCTA AGGGGCGTAT TTTAGAGCCT GCTAAAGAAA ACGAGCAAGG CTTTTTAGAA GCTCAAATAA GCCCTATTGA ATATTTAGAA TACGATAAAG AAAACATTCA AGCGATCGTG GAAGTCTTAA AAGAAAAAGT GATCACTCTA GCCAATGTCA GCTCACTCTT TCCTCCGGAT TTGATCAAGG CTTTAGAAGA CAATGACGAT CCTAACCGCA TCGCTGATTT AATCGCAGCG GCCTTGCATT TGAAAAGAGA TCAAGCGTAT TCTCTTTTTG CGAACAACAA CACCGAACAG CGCTTGTTGG ATTTGATTGA TATTGTGATA GAAGAGACTA AAACCCAAAA ACTCCAAAAA GAAATCAAAT CCAAAGTCCA TCAAAAAATG GAGCAAACCA ATAAGGAATA TTTCTTAAAA GAGCAGCTCA AACAAATCCA AAAAGAGCTT GGCACAGACA AACAGCGAGA TGAAGATTTA AACCAATACT ACCAAAAACT AGAAAGCATC AAGCCTTTCT TAAAAGAAGA AGCGTTTAAG GAGATTAAAA AGCAAATTGA CCGGCTGAGC CGAACCCATG CGGATAGTTC AGATAGCGCG ACTTTACAAA ATTATATTGA AACCATGTTA GATGTGCCTT TTGGGCAATA CGGGAAAAAA GCGCTTGACA TTAAGCATGT GAGAGAACAA CTAGACAAAG ATCATTATTC CTTAAAAAGG CCTAAAGAGC GTATTGTAGA ATACTTTGCC ACCATGCAGC TTTTAGAAAT GCGCCGCAAG AAAAAGCCAG AAAAAAAAGA CAAAACTAAA GGCACGATTT TATGCTTTTA TGGGCCTCCT GGCGTGGGTA AAACGAGCTT GGCTAATTCC ATCGCTAAAG CGATAGAGCG CCCTTTAGTT CGGATCGCTT TAGGGGGCTT AGAAGATGTG AATGAATTAA GAGGGCACAG ACGCACTTAT ATAGGCTCCA TGCCCGGGCG CATTGTCCAA GGGCTCATTG AAGCCAAAAA GATGAATCCG GTCATGGTTT TAGATGAAAT TGATAAGGTG GATAGAAGCG TTAGAGGCGA TCCAGCGAGC GCTTTATTAG AGATCTTAGA CCCTGAGCAA AATATCGCTT TTAGGGATCA TTATGCGAAT TTCAGCATTG ATTTATCGCA AGTGATTTTT ATCGCTACCG CTAATAATAT TGACAGGATC CCAGCCCCTT TAAGAGACAG AATGGAATTT ATCAGCGTGT CCAGCTACAC GCCTAGCGAA AAAGAAGAGA TCGCTAAAAA CTACCTCATC CCCCAAGAAT TAGAAAAGCA CGCCTTAAAG CCTAGCGAAG TGGATATTAG CCATGAGTGT TTGAAACTCA TTATTGAAAA ATACACCAGA GAAGCGGGCG TTAGGGATTT ACGAAGACAG ATCGCAACGA TTATGCGTAA AGCGGCTTTA AAATACCTAG AAGATAACCC GCACAAAAAA GGGCGAACTA AAAAAGGCGA GGATAAAAAA AGCGAAAATC AAAGAGACGA GAACAAAGAT TTCTGCGTCT CTATCACGCC TGATAACCTT AAAGAGTATT TAGAGCGCAT GGTGTTTGAA ATTGACCCCA TAGATGAAGA AAATAAAATC GGTATTATCA ATGGTTTGGC ATGGACTCCA GTGGGCGGTG ATGTGCTTAA AATTGAAGCG GTTAAGATCA GGGGTAAGGG GGAATTGAAA CTCACCGGGA GTTTAGGCGA TGTGATGAAA GAATCCGCCA TTATTGCCTT TTCTGTTGTC AAAGTCTTAT TGGATAACGA AACCTTAAAA GCGCCTAAAA TCCCTAGCGA GACCGATGCA GAGGGCAAGA AAAAGAAAAA AGCGCTGAAA GTTTATAACG CTTATGATTT GCACTTGCAT GTCCCTGAGG GGGCTACGCC TAAAGACGGC CCGAGCGCTG GGATCGCTAT GGCGAGCGTG ATGGCGAGCA TTTTGTGCGA CAGGGCTACA AGAAGCGAAG TGGCAATGAC GGGCGAATTG ACTTTGAGCG GGGAAGTTTT ACCCATAGGA GGGTTGAAAG AAAAATTGAT TGCCGCTTTT AAAGCCGGCA TTAAAACCGC TCTCATTCCT GTCAAAAATT ACGAAAGGGA TTTAGACGAG ATCCCTGCTG AAGTGCGAGA GAATTTAAAC ATCGTTGCGG TGAAAAACAT CGCTGAAGTG TTAGAAAAAA CCTTACTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT